(Circular) (Six-base) MAPSORT of: pFN21AE5586.seq Check: 6278 from: 1 to: 5818 pFN21AE5586 5818 bp With 182 enzymes: SgfI * January 30, 2014 17:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3628 278 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2789 3161 1937 Size: 852 372 4594 Fragments arranged by size: 4594 852 372 Acc65I G'GTAC_C Cuts at: 3139 3139 Size: 5818 AclI AA'CG_TT Cuts at: 1563 3945 4318 1563 Size: 2382 373 3063 Fragments arranged by size: 3063 2382 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2425 2957 3881 5265 5533 1632 Size: 793 532 924 1384 268 1917 Fragments arranged by size: 1917 1384 924 793 532 268 AfeI AGC'GCT Cuts at: 2295 2295 Size: 5818 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5621 AflIII A'CryG_T Cuts at: 1203 4717 5694 1203 Size: 3514 977 1327 Fragments arranged by size: 3514 1327 977 AgeI A'CCGG_T Cuts at: 4649 4649 Size: 5818 AhdI GACnn_n'nnGTC Cuts at: 4547 4547 Size: 5818 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5818 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2984 1184 Size: 1800 4018 AlwNI CAG_nnn'CTG Cuts at: 2104 2189 2746 2845 3561 5133 2104 Size: 85 557 99 716 1572 2789 Fragments arranged by size: 2789 1572 716 557 99 85 ApaLI G'TGCA_C Cuts at: 3875 5031 3875 Size: 1156 4662 ApoI r'AATT_y Cuts at: 1493 3127 3353 3505 5614 1493 Size: 1634 226 152 2109 1697 Fragments arranged by size: 2109 1697 1634 226 152 AseI AT'TA_AT Cuts at: 160 1032 4372 160 Size: 872 3340 1606 Fragments arranged by size: 3340 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 AvaI C'yCGr_G Cuts at: 1870 1943 2280 2468 2594 3143 1870 Size: 73 337 188 126 549 4545 Fragments arranged by size: 4545 549 337 188 126 73 BamHI G'GATC_C Cuts at: 2212 2590 2937 3148 3515 2212 Size: 378 347 211 367 4515 Fragments arranged by size: 4515 378 367 347 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2345 3139 4594 618 Size: 325 217 627 558 794 1455 1842 Fragments arranged by size: 1842 1455 794 627 558 325 217 BanII G_rGCy'C Cuts at: 1391 2582 3073 3137 1391 Size: 1191 491 64 4072 Fragments arranged by size: 4072 1191 491 64 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2971 928 Size: 971 1072 3775 Fragments arranged by size: 3775 1072 971 BbvCI CC'TCA_GC Cuts at: 2387 2387 Size: 5818 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4009 4043 1903 Size: 34 2072 34 3678 Fragments arranged by size: 3678 2072 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3710 4920 3710 Size: 1210 4608 BclI T'GATC_A Cuts at: 1548 1548 Size: 5818 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2056 2091 2657 Size: 107 122 71 631 605 384 35 566 Cuts at: 2657 4429 136 Size: 1772 1525 Fragments arranged by size: 1772 1525 631 605 566 384 122 107 71 35 BglII A'GATC_T Cuts at: 5813 5813 Size: 5818 BlpI GC'TnA_GC Cuts at: 2576 3235 2576 Size: 659 5159 Bme1580I G_kGCm'C Cuts at: 1790 2052 2232 2910 3879 5035 1790 Size: 262 180 678 969 1156 2573 Fragments arranged by size: 2573 1156 969 678 262 180 BmgBI CAC'GTC Cuts at: 1318 2257 1318 Size: 939 4879 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3549 4507 442 Size: 416 53 83 399 24 2132 958 1753 Fragments arranged by size: 2132 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2141 1056 Size: 1085 4733 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2211 2423 2853 3038 4478 1239 Size: 972 212 430 185 1440 2579 Fragments arranged by size: 2579 1440 972 430 212 185 Bpu10I CC'TnA_GC Cuts at: 2387 2387 Size: 5818 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3943 4808 5106 3943 Size: 865 298 4655 Fragments arranged by size: 4655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4481 882 Size: 617 2982 2219 Fragments arranged by size: 2982 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2047 2779 3609 493 Size: 711 843 732 830 2702 Fragments arranged by size: 2702 843 830 732 711 BsaBI GATnn'nnATC Cuts at: 5812 5812 Size: 5818 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2265 3625 4007 4642 Size: 53 83 186 936 732 1360 382 635 Cuts at: 4642 275 Size: 1451 Fragments arranged by size: 1451 1360 936 732 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4251 4649 4923 5070 5481 4251 Size: 398 274 147 411 4588 Fragments arranged by size: 4588 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5593 Fragments arranged by size: 5593 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5818 BseYI C'CCAG_C Cuts at: 2231 2296 2599 2661 5021 5517 2231 Size: 65 303 62 2360 496 2532 Fragments arranged by size: 2532 2360 496 303 65 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2491 1163 Size: 573 755 4490 Fragments arranged by size: 4490 755 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3268 4029 5057 1540 Size: 275 186 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3073 3879 3964 5035 3073 Size: 806 85 1071 3856 Fragments arranged by size: 3856 1071 806 85 BsmI GAATG_Cn' Cuts at: 3329 3422 3329 Size: 93 5725 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2052 2232 2582 2910 3073 3137 3879 Size: 399 262 180 350 328 163 64 742 Cuts at: 3879 3964 5035 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 350 328 262 180 163 85 64 BspEI T'CCGG_A Cuts at: 5481 5481 Size: 5818 BspHI T'CATG_A Cuts at: 3707 5437 3707 Size: 1730 4088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 3173 844 Size: 1241 1088 3489 Fragments arranged by size: 3489 1241 1088 BsrBI CCG'CTC Cuts at: 1394 1823 3705 1394 Size: 429 1882 3507 Fragments arranged by size: 3507 1882 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4313 4487 65 Size: 1183 3065 174 1396 Fragments arranged by size: 3065 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2267 2378 4462 4649 1700 Size: 110 457 111 2084 187 2869 Fragments arranged by size: 2869 2084 457 187 111 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5818 BssHII G'CGCG_C Cuts at: 1925 2407 1925 Size: 482 5336 BssSI C'ACGA_G Cuts at: 2909 3878 4890 2909 Size: 969 1012 3837 Fragments arranged by size: 3837 1012 969 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5818 BstBI TT'CG_AA Cuts at: 4622 4622 Size: 5818 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5818 BstYI r'GATC_y Cuts at: 1972 2212 2590 2892 2937 3148 3515 3900 3917 Size: 240 378 302 45 211 367 385 17 Cuts at: 3917 5369 5455 5467 5813 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 378 367 346 302 240 211 86 45 17 12 BstZ17I GTA'TAC Cuts at: 2790 2790 Size: 5818 BtgI C'CryG_G Cuts at: 513 1065 2004 2361 2419 513 Size: 552 939 357 58 3912 Fragments arranged by size: 3912 939 552 357 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2020 487 Size: 683 56 794 4285 Fragments arranged by size: 4285 794 683 56 BtsI GCAGTG_nn' Cuts at: 3334 4128 4148 3334 Size: 794 20 5004 Fragments arranged by size: 5004 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5818 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3031 3066 529 Size: 35 2467 35 3281 Fragments arranged by size: 3281 2467 35 35 DraI TTT'AAA Cuts at: 3122 3122 Size: 5818 DraIII CAC_nnn'GTG Cuts at: 2227 2227 Size: 5818 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4825 809 Size: 507 3509 1802 Fragments arranged by size: 3509 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3265 4154 Size: 54 1425 50 275 18 177 1258 889 Cuts at: 4154 5783 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1258 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3265 1537 Size: 275 1453 4090 Fragments arranged by size: 4090 1453 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2093 3748 1353 Size: 740 1655 3423 Fragments arranged by size: 3423 1655 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2276 4405 4779 4925 387 Size: 1064 138 687 2129 374 146 1280 Fragments arranged by size: 2129 1280 1064 687 374 146 138 EcoICRI GAG'CTC Cuts at: 3071 3071 Size: 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2211 2423 2425 2853 2957 3038 3881 Size: 393 579 212 2 428 104 81 843 Cuts at: 3881 4478 5265 5533 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 843 787 597 579 428 393 268 212 104 81 2 EcoRI G'AATT_C Cuts at: 3127 3505 3127 Size: 378 5440 FspI TGC'GCA Cuts at: 2147 4324 2147 Size: 2177 3641 HaeII r_GCGC'y Cuts at: 1678 2086 2238 2274 2297 4965 1678 Size: 408 152 36 23 2668 2531 Fragments arranged by size: 2668 2531 408 152 36 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2922 2954 4459 4491 4533 Size: 32 135 32 1350 32 1505 32 42 Cuts at: 4533 4565 1373 Size: 32 2626 Fragments arranged by size: 2626 1505 1350 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3162 3408 669 Size: 988 281 1224 246 3079 Fragments arranged by size: 3079 1224 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5818 HpaI GTT'AAC Cuts at: 3408 3408 Size: 5818 KpnI G_GTAC'C Cuts at: 3143 3143 Size: 5818 MfeI C'AATT_G Cuts at: 3417 3417 Size: 5818 MluI A'CGCG_T Cuts at: 5694 5694 Size: 5818 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4932 5116 1305 Size: 3627 184 2007 Fragments arranged by size: 3627 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3996 Fragments arranged by size: 3996 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3776 4135 4294 518 Size: 648 2610 359 159 2042 Fragments arranged by size: 2610 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2151 2200 2421 2575 3234 3911 5059 5304 Size: 545 49 221 154 659 677 1148 245 Cuts at: 5304 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 659 545 245 221 154 49 NaeI GCC'GGC Cuts at: 2269 2380 2269 Size: 111 5707 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4327 Fragments arranged by size: 4327 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5818 NgoMIV G'CCGG_C Cuts at: 2267 2378 2267 Size: 111 5707 NheI G'CTAG_C Cuts at: 1052 2137 1052 Size: 1085 4733 NotI GC'GGCC_GC Cuts at: 3265 3265 Size: 5818 NspI r_CATG'y Cuts at: 1132 1229 3176 4721 1132 Size: 97 1947 1545 2229 Fragments arranged by size: 2229 1947 1545 97 PasI CC'CwG_GG Cuts at: 1348 2855 1348 Size: 1507 4311 PciI A'CATG_T Cuts at: 4717 4717 Size: 5818 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 2845 1108 Size: 996 741 4081 Fragments arranged by size: 4081 996 741 PfoI T'CCnGG_A Cuts at: 3015 3015 Size: 5818 PmeI GTTT'AAAC Cuts at: 3122 3122 Size: 5818 PmlI CAC'GTG Cuts at: 2047 2779 2047 Size: 732 5086 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3887 3919 5400 5432 3887 Size: 32 1481 32 4273 Fragments arranged by size: 4273 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5818 PsiI TTA'TAA Cuts at: 3388 3388 Size: 5818 PstI C_TGCA'G Cuts at: 830 2082 2306 2658 2775 2905 3170 830 Size: 1252 224 352 117 130 265 3478 Fragments arranged by size: 3478 1252 352 265 224 130 117 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5818 SacI G_AGCT'C Cuts at: 3073 3073 Size: 5818 SacII CC_GC'GG Cuts at: 2422 2422 Size: 5818 SalI G'TCGA_C Cuts at: 1936 3160 1936 Size: 1224 4594 SbfI CC_TGCA'GG Cuts at: 2658 3170 2658 Size: 512 5306 ScaI AGT'ACT Cuts at: 4066 5778 4066 Size: 1712 4106 SexAI A'CCwGG_T Cuts at: 2730 2730 Size: 5818 SfcI C'TryA_G Cuts at: 826 1045 2078 2302 2654 2771 2901 3166 4301 Size: 219 1033 224 352 117 130 265 1135 Cuts at: 4301 4982 5173 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1033 681 352 265 224 219 191 130 117 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5818 SgrAI Cr'CCGG_yG Cuts at: 1700 2267 1700 Size: 567 5251 SmaI CCC'GGG Cuts at: 1872 2282 2470 3145 1872 Size: 410 188 675 4545 Fragments arranged by size: 4545 675 410 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 3922 4823 5085 820 Size: 197 926 1979 901 262 1553 Fragments arranged by size: 1979 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3609 493 Size: 3116 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5818 SphI G_CATG'C Cuts at: 1132 3176 1132 Size: 2044 3774 SspI AAT'ATT Cuts at: 5 52 3742 5 Size: 47 3690 2081 Fragments arranged by size: 3690 2081 47 StuI AGG'CCT Cuts at: 1517 2769 1517 Size: 1252 4566 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 3257 513 Size: 552 939 495 758 3074 Fragments arranged by size: 3074 939 758 552 495 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3845 3862 4015 4200 4645 325 Size: 1255 2265 17 153 185 445 1498 Fragments arranged by size: 2265 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4064 5776 96 Size: 274 80 33 51 1443 2087 1712 138 Fragments arranged by size: 2087 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5778 5810 5778 Size: 32 5786 Tth111I GACn'n_nGTC Cuts at: 1316 2159 1316 Size: 843 4975 XbaI T'CTAG_A Cuts at: 3154 3154 Size: 5818 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2359 2689 2818 2359 Size: 330 129 5359 Fragments arranged by size: 5359 330 129 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5818 XmaI C'CCGG_G Cuts at: 1870 2280 2468 3143 1870 Size: 410 188 675 4545 Fragments arranged by size: 4545 675 410 188 XmnI GAAnn'nnTTC Cuts at: 3947 3947 Size: 5818 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3626 276 Size: 53 83 186 936 2092 2468 Fragments arranged by size: 2468 2092 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SanDI SapI SfiI SfoI SrfI SwaI