(Linear) (Six-base) MAPSORT of: KIEE5586.seq Check: 7525 from: 1 to: 1113 KIEE5586.orf With 182 enzymes: SgfI * January 29, 2014 12:06 .. AccI GT'mk_AC Cuts at: 0 784 1113 Size: 784 329 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 420 952 1113 Size: 420 532 161 Fragments arranged by size: 532 420 161 AfeI AGC'GCT Cuts at: 0 290 1113 Size: 290 823 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 979 1113 Size: 979 134 AlwNI CAG_nnn'CTG Cuts at: 0 99 184 741 840 1113 Size: 99 85 557 99 273 Fragments arranged by size: 557 273 99 99 85 AvaI C'yCGr_G Cuts at: 0 275 463 589 1113 Size: 275 188 126 524 Fragments arranged by size: 524 275 188 126 BamHI G'GATC_C Cuts at: 0 207 585 932 1113 Size: 207 378 347 181 Fragments arranged by size: 378 347 207 181 BanI G'GyrC_C Cuts at: 0 340 1113 Size: 340 773 BanII G_rGCy'C Cuts at: 0 577 1068 1113 Size: 577 491 45 Fragments arranged by size: 577 491 45 BbsI GAAGACnn'nnnn_ Cuts at: 0 966 1113 Size: 966 147 BbvCI CC'TCA_GC Cuts at: 0 382 1113 Size: 382 731 BglI GCCn_nnn'nGGC Cuts at: 0 51 86 652 1113 Size: 51 35 566 461 Fragments arranged by size: 566 461 51 35 BlpI GC'TnA_GC Cuts at: 0 571 1113 Size: 571 542 Bme1580I G_kGCm'C Cuts at: 0 47 227 905 1113 Size: 47 180 678 208 Fragments arranged by size: 678 208 180 47 BmgBI CAC'GTC Cuts at: 0 252 1113 Size: 252 861 BmtI G_CTAG'C Cuts at: 0 136 1113 Size: 136 977 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 418 848 1033 1113 Size: 206 212 430 185 80 Fragments arranged by size: 430 212 206 185 80 Bpu10I CC'TnA_GC Cuts at: 0 382 1113 Size: 382 731 BsaAI yAC'GTr Cuts at: 0 42 774 1113 Size: 42 732 339 Fragments arranged by size: 732 339 42 BsaHI Gr'CG_yC Cuts at: 0 260 1113 Size: 260 853 BseYI C'CCAG_C Cuts at: 0 226 291 594 656 1113 Size: 226 65 303 62 457 Fragments arranged by size: 457 303 226 65 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 486 1113 Size: 486 627 BsiHKAI G_wGCw'C Cuts at: 0 1068 1113 Size: 1068 45 Bsp1286I G_dGCh'C Cuts at: 0 47 227 577 905 1068 1113 Size: 47 180 350 328 163 45 Fragments arranged by size: 350 328 180 163 47 45 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 80 1113 Size: 80 1033 BsrFI r'CCGG_y Cuts at: 0 262 373 1113 Size: 262 111 740 Fragments arranged by size: 740 262 111 BssHII G'CGCG_C Cuts at: 0 402 1113 Size: 402 711 BssSI C'ACGA_G Cuts at: 0 904 1113 Size: 904 209 BstYI r'GATC_y Cuts at: 0 207 585 887 932 1113 Size: 207 378 302 45 181 Fragments arranged by size: 378 302 207 181 45 BstZ17I GTA'TAC Cuts at: 0 785 1113 Size: 785 328 BtgI C'CryG_G Cuts at: 0 356 414 1113 Size: 356 58 699 Fragments arranged by size: 699 356 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 15 1113 Size: 15 1098 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1026 1061 1113 Size: 1026 35 52 Fragments arranged by size: 1026 52 35 DraIII CAC_nnn'GTG Cuts at: 0 222 1113 Size: 222 891 EaeI y'GGCC_r Cuts at: 0 2 1113 Size: 2 1111 EarI CTCTTCn'nnn_ Cuts at: 0 88 1113 Size: 88 1025 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 271 1113 Size: 271 842 EcoICRI GAG'CTC Cuts at: 0 1066 1113 Size: 1066 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 206 418 420 848 952 1033 1113 Size: 206 212 2 428 104 81 80 Fragments arranged by size: 428 212 206 104 81 80 2 FspI TGC'GCA Cuts at: 0 142 1113 Size: 142 971 HaeII r_GCGC'y Cuts at: 0 81 233 269 292 1113 Size: 81 152 36 23 821 Fragments arranged by size: 821 152 81 36 23 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 917 949 1113 Size: 917 32 164 Fragments arranged by size: 917 164 32 MspA1I CmG'CkG Cuts at: 0 146 195 416 570 1113 Size: 146 49 221 154 543 Fragments arranged by size: 543 221 154 146 49 NaeI GCC'GGC Cuts at: 0 264 375 1113 Size: 264 111 738 Fragments arranged by size: 738 264 111 NgoMIV G'CCGG_C Cuts at: 0 262 373 1113 Size: 262 111 740 Fragments arranged by size: 740 262 111 NheI G'CTAG_C Cuts at: 0 132 1113 Size: 132 981 PasI CC'CwG_GG Cuts at: 0 850 1113 Size: 850 263 PflMI CCAn_nnn'nTGG Cuts at: 0 99 840 1113 Size: 99 741 273 Fragments arranged by size: 741 273 99 PfoI T'CCnGG_A Cuts at: 0 1010 1113 Size: 1010 103 PmlI CAC'GTG Cuts at: 0 42 774 1113 Size: 42 732 339 Fragments arranged by size: 732 339 42 PstI C_TGCA'G Cuts at: 0 77 301 653 770 900 1113 Size: 77 224 352 117 130 213 Fragments arranged by size: 352 224 213 130 117 77 SacI G_AGCT'C Cuts at: 0 1068 1113 Size: 1068 45 SacII CC_GC'GG Cuts at: 0 417 1113 Size: 417 696 SbfI CC_TGCA'GG Cuts at: 0 653 1113 Size: 653 460 SexAI A'CCwGG_T Cuts at: 0 725 1113 Size: 725 388 SfcI C'TryA_G Cuts at: 0 73 297 649 766 896 1113 Size: 73 224 352 117 130 217 Fragments arranged by size: 352 224 217 130 117 73 SgrAI Cr'CCGG_yG Cuts at: 0 262 1113 Size: 262 851 SmaI CCC'GGG Cuts at: 0 277 465 1113 Size: 277 188 648 Fragments arranged by size: 648 277 188 StuI AGG'CCT Cuts at: 0 764 1113 Size: 764 349 StyI C'CwwG_G Cuts at: 0 494 1113 Size: 494 619 Tth111I GACn'n_nGTC Cuts at: 0 154 1113 Size: 154 959 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 354 684 813 1113 Size: 354 330 129 300 Fragments arranged by size: 354 330 300 129 XmaI C'CCGG_G Cuts at: 0 275 463 1113 Size: 275 188 650 Fragments arranged by size: 650 275 188 Enzymes that do cut: AccI AcuI AfeI AlfI AlwNI AvaI BamHI BanI BanII BbsI BbvCI BglI BlpI Bme1580I BmgBI BmtI BpmI Bpu10I BsaAI BsaHI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstYI BstZ17I BtgI BtgZI CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI FspI HaeII Hin4I MspA1I NaeI NgoMIV NheI PasI PflMI PfoI PmlI PstI SacI SacII SbfI SexAI SfcI SgrAI SmaI StuI StyI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglII BmrI BplI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstXI Bsu36I BtsI ClaI DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI NarI NcoI NdeI NotI NruI NsiI NspI PacI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SalI SanDI SapI ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI TstI XbaI XhoI XmnI ZraI