################################## # NO CUT ENZYMES ( 127 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflIII AgeI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BalI BamHI BanII BbeI BclI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NarI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tth111I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccII 1328 CGCG CG^CG AcsI 762 GAATTC [GA]^AATT[CT] AflII 424 CTTAAG C^TTAAG AhdI 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1597 GTGCAC G^TGCAC ApaLI 1597 GTGCAC G^TGCAC ApoI 762 GAATTC [GA]^AATT[CT] AspEI 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1597 GTGCAC G[AT]GC[AT]^C BbrPI 1594 CACGTG CAC^GTG BbsI 1416 GAAGAC GAAGAC(2/6) BcgI 1512 GCAGCTATCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1088 GGATAC GTATCC(6/5) BfrI 424 CTTAAG C^TTAAG BmyI 1597 GTGCAC G[AGT]GC[ACT]^C BpuAI 1416 GAAGAC GAAGAC(2/6) BsaAI 1594 CACGTG [CT]AC^GT[GA] BsaI 1676 GAGACC GGTCTC(1/5) BsiHKAI 1597 GTGCAC G[AT]GC[AT]^C BsmI 945 GAATGC GAATGC(1/-1) Bsp1286I 1597 GTGCAC G[AGT]GC[ACT]^C BsrDI 1382 GCAATG GCAATG(2/0) BssSI 1520 CTCGTG CACGAG(-5/-1) BstUI 1328 CGCG CG^CG BstYI 1688 GGATCT [GA]^GATC[CT] EaeI 413 CGGCCA [CT]^GGCC[GA] Eam1105I 439 GACACAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 768 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 762 GAATTC G^AATTC HaeII 1668 AGCGCC [GA]GCGC^[CT] HincII 160 GTTGAC GT[CT]^[GA]AC HindII 160 GTTGAC GT[CT]^[GA]AC MflI 1688 GGATCT [GA]^GATC[CT] MseI 425 TTAA T^TAA MvnI 1328 CGCG CG^CG NaeI 411 GCCGGC GCC^GGC NcoI 782 CCATGG C^CATGG NdeI 715 CATATG CA^TATG NgoMIV 411 GCCGGC G^CCGGC NheI 738 GCTAGC G^CTAGC PaeR7I 753 CTCGAG C^TCGAG PmaCI 1594 CACGTG CAC^GTG PmlI 1594 CACGTG CAC^GTG PpuMI 683 AGGTCCT [GA]G^G[AT]CC[CT] PstI 706 CTGCAG CTGCA^G SfcI 706 CTGCAG C^T[GA][CT]AG Tru9I 425 TTAA T^TAA Tsp45I 1465 GTGAC ^GT[GC]AC XbaI 309 TCTAGA T^CTAGA XhoI 753 CTCGAG C^TCGAG XhoII 1688 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AlwI 609 GGATC GGATC(4/5) 1688 GGATC AvaI 753 CTCGAG C^[CT]CG[GA]G 1490 CTCGGG BsgI 1248 CTGCAC GTGCAG(16/14) 1143 GTGCAG BsoBI 753 CTCGAG C^[CT]CG[GA]G 1490 CTCGGG BspMI 1420 ACCTGC ACCTGC(4/8) 1679 ACCTGC BsrFI 411 GCCGGC [GA]^CCGG[CT] 929 ACCGGC BstXI 50 CCAACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1100 CCACTCTCCTGG Cfr10I 411 GCCGGC [GA]^CCGG[CT] 929 ACCGGC DraII 659 AGGCCCC [GA]G^G[ACGT]CC[CT] 683 AGGTCCT DsaI 89 CCACGG C^C[GA][CT]GG 782 CCATGG EcoO109I 659 AGGCCCC [GA]G^G[ACGT]CC[CT] 683 AGGTCCT EcoT14I 782 CCATGG C^C[AT][AT]GG 1274 CCAAGG MaeIII 1465 GTGAC ^GT[ACGT]AC 1532 GTTAC MslI 340 CATAGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1545 CATCTGCATG PvuII 502 CAGCTG CAG^CTG 1460 CAGCTG SmlI 424 CTTAAG C^T[CT][GA]AG 753 CTCGAG StyI 782 CCATGG C^C[AT][AT]GG 1274 CCAAGG TfiI 336 GAATC G^A[AT]TC 1456 GATTC XcmI 219 CCATCGGCAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1097 CCACCACTCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AfaI AluI AlwNI AvaII BanI BbvCI BbvI BcnI BfaI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI Tsp509I TspRI TthHB8I