(Circular) (Six-base) MAPSORT of: pFN21AE3243.seq Check: 9792 from: 1 to: 6403 pFN21AE3243 6403 bp With 182 enzymes: SgfI * March 28, 2012 14:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4213 278 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3746 1937 Size: 1809 4594 Acc65I G'GTAC_C Cuts at: 3724 3724 Size: 6403 AclI AA'CG_TT Cuts at: 1563 4530 4903 1563 Size: 2967 373 3063 Fragments arranged by size: 3063 2967 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2798 3509 4466 5850 6118 1632 Size: 1166 711 957 1384 268 1917 Fragments arranged by size: 1917 1384 1166 957 711 268 AflII C'TTAA_G Cuts at: 820 1017 2429 820 Size: 197 1412 4794 Fragments arranged by size: 4794 1412 197 AflIII A'CryG_T Cuts at: 1203 5302 6279 1203 Size: 4099 977 1327 Fragments arranged by size: 4099 1327 977 AgeI A'CCGG_T Cuts at: 5234 5234 Size: 6403 AhdI GACnn_n'nnGTC Cuts at: 2449 5132 2449 Size: 2683 3720 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6403 AlwNI CAG_nnn'CTG Cuts at: 2692 2734 3239 4146 5718 2692 Size: 42 505 907 1572 3377 Fragments arranged by size: 3377 1572 907 505 42 ApaLI G'TGCA_C Cuts at: 3602 4460 5616 3602 Size: 858 1156 4389 Fragments arranged by size: 4389 1156 858 ApoI r'AATT_y Cuts at: 1493 2767 3712 3938 4090 6199 1493 Size: 1274 945 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 945 226 152 AseI AT'TA_AT Cuts at: 160 1032 4957 160 Size: 872 3925 1606 Fragments arranged by size: 3925 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 AvaI C'yCGr_G Cuts at: 1870 1943 2758 3495 3728 1870 Size: 73 815 737 233 4545 Fragments arranged by size: 4545 815 737 233 73 BamHI G'GATC_C Cuts at: 3733 4100 3733 Size: 367 6036 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2239 2798 3635 3724 5179 Size: 325 217 627 452 559 837 89 1455 Cuts at: 5179 618 Size: 1842 Fragments arranged by size: 1842 1455 837 627 559 452 325 217 89 BanII G_rGCy'C Cuts at: 1391 3722 1391 Size: 2331 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3428 928 Size: 971 1529 3903 Fragments arranged by size: 3903 1529 971 BbvCI CC'TCA_GC Cuts at: 2100 2696 3018 2100 Size: 596 322 5485 Fragments arranged by size: 5485 596 322 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3506 3540 4594 4628 1903 Size: 34 1569 34 1054 34 3678 Fragments arranged by size: 3678 1569 1054 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3087 4295 5505 3087 Size: 1208 1210 3985 Fragments arranged by size: 3985 1210 1208 BclI T'GATC_A Cuts at: 1548 1548 Size: 6403 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5014 136 Size: 107 122 71 631 605 3342 1525 Fragments arranged by size: 3342 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6398 6398 Size: 6403 BlpI GC'TnA_GC Cuts at: 3820 3820 Size: 6403 Bme1580I G_kGCm'C Cuts at: 1790 3606 4464 5620 1790 Size: 1816 858 1156 2573 Fragments arranged by size: 2573 1816 1156 858 BmgBI CAC'GTC Cuts at: 1318 2176 1318 Size: 858 5545 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2559 2697 4134 Size: 416 53 83 399 24 1142 138 1437 Cuts at: 4134 5092 442 Size: 958 1753 Fragments arranged by size: 1753 1437 1142 958 416 399 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2747 1056 Size: 1691 4712 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2756 2778 3230 5063 1239 Size: 1517 22 452 1833 2579 Fragments arranged by size: 2579 1833 1517 452 22 Bpu10I CC'TnA_GC Cuts at: 2100 2696 3018 2100 Size: 596 322 5485 Fragments arranged by size: 5485 596 322 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4528 5393 5691 4528 Size: 865 298 5240 Fragments arranged by size: 5240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3675 5066 882 Size: 617 2176 1391 2219 Fragments arranged by size: 2219 2176 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 3601 4194 493 Size: 711 2397 593 2702 Fragments arranged by size: 2702 2397 711 593 BsaBI GATnn'nnATC Cuts at: 6397 6397 Size: 6403 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4210 4592 5227 275 Size: 53 83 186 936 2677 382 635 1451 Fragments arranged by size: 2677 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4836 5234 5508 5655 6066 4836 Size: 398 274 147 411 5173 Fragments arranged by size: 5173 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2271 2301 2437 2467 3059 Size: 30 165 30 868 30 136 30 592 Cuts at: 3059 3089 1178 Size: 30 4492 Fragments arranged by size: 4492 868 592 165 136 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3080 3278 3626 1186 Size: 1894 198 348 3963 Fragments arranged by size: 3963 1894 348 198 BseYI C'CCAG_C Cuts at: 2071 3326 5606 6102 2071 Size: 1255 2280 496 2372 Fragments arranged by size: 2372 2280 1255 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3169 3238 1163 Size: 573 1433 69 4328 Fragments arranged by size: 4328 1433 573 69 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3853 4614 5642 1540 Size: 275 186 1852 761 1028 2301 Fragments arranged by size: 2301 1852 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3606 4464 4549 5620 3606 Size: 858 85 1071 4389 Fragments arranged by size: 4389 1071 858 85 BsmI GAATG_Cn' Cuts at: 2956 3914 4007 2956 Size: 958 93 5352 Fragments arranged by size: 5352 958 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3606 3722 4464 4549 5620 1391 Size: 399 1816 116 742 85 1071 2174 Fragments arranged by size: 2174 1816 1071 742 399 116 85 BspEI T'CCGG_A Cuts at: 6066 6066 Size: 6403 BspHI T'CATG_A Cuts at: 4292 6022 4292 Size: 1730 4673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3434 3693 3758 844 Size: 2590 259 65 3489 Fragments arranged by size: 3489 2590 259 65 BsrBI CCG'CTC Cuts at: 1394 1823 4290 1394 Size: 429 2467 3507 Fragments arranged by size: 3507 2467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3394 4898 5072 65 Size: 1183 2146 1504 174 1396 Fragments arranged by size: 2146 1504 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2416 2934 5047 5234 1700 Size: 110 606 518 2113 187 2869 Fragments arranged by size: 2869 2113 606 518 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6403 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6403 BssSI C'ACGA_G Cuts at: 3525 4463 5475 3525 Size: 938 1012 4453 Fragments arranged by size: 4453 1012 938 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6403 BstBI TT'CG_AA Cuts at: 5207 5207 Size: 6403 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6403 BstXI CCAn_nnnn'nTGG Cuts at: 2062 3112 2062 Size: 1050 5353 BstYI r'GATC_y Cuts at: 1972 3693 3733 4100 4485 4502 5954 6040 6052 Size: 1721 40 367 385 17 1452 86 12 Cuts at: 6052 6398 1972 Size: 346 1977 Fragments arranged by size: 1977 1721 1452 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2094 2787 513 Size: 552 939 90 693 4129 Fragments arranged by size: 4129 939 693 552 90 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5664 Fragments arranged by size: 5664 683 56 BtsI GCAGTG_nn' Cuts at: 3604 3919 4713 4733 3604 Size: 315 794 20 5274 Fragments arranged by size: 5274 794 315 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6368 DraI TTT'AAA Cuts at: 3707 3707 Size: 6403 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5410 809 Size: 507 4094 1802 Fragments arranged by size: 4094 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2418 3850 4739 Size: 54 1425 50 275 18 588 1432 889 Cuts at: 4739 6368 8 Size: 1629 43 Fragments arranged by size: 1629 1432 1425 889 588 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3850 1537 Size: 275 2038 4090 Fragments arranged by size: 4090 2038 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4333 1353 Size: 2980 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4990 5364 5510 387 Size: 1064 138 3401 374 146 1280 Fragments arranged by size: 3401 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2756 2778 2798 3230 3509 4466 5063 Size: 393 1124 22 20 432 279 957 597 Cuts at: 5063 5850 6118 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1124 957 787 597 432 393 279 268 22 20 EcoNI CCTnn'n_nnAGG Cuts at: 2777 2777 Size: 6403 EcoO109I rG'GnC_Cy Cuts at: 2665 2689 2665 Size: 24 6379 EcoRI G'AATT_C Cuts at: 2767 3712 4090 2767 Size: 945 378 5080 Fragments arranged by size: 5080 945 378 FspI TGC'GCA Cuts at: 4909 4909 Size: 6403 HaeII r_GCGC'y Cuts at: 1678 3677 5550 1678 Size: 1999 1873 2531 Fragments arranged by size: 2531 1999 1873 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2027 2059 2435 2467 3362 Size: 32 135 32 455 32 376 32 895 Cuts at: 3362 3394 3551 3583 5044 5076 5118 5150 1373 Size: 32 157 32 1461 32 42 32 2626 Fragments arranged by size: 2626 1461 895 455 376 157 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2167 3747 3993 669 Size: 988 281 229 1580 246 3079 Fragments arranged by size: 3079 1580 988 281 246 229 HindIII A'AGCT_T Cuts at: 748 748 Size: 6403 HpaI GTT'AAC Cuts at: 3993 3993 Size: 6403 KpnI G_GTAC'C Cuts at: 3728 3728 Size: 6403 MfeI C'AATT_G Cuts at: 4002 4002 Size: 6403 MluI A'CGCG_T Cuts at: 6279 6279 Size: 6403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2876 5517 5701 1305 Size: 1571 2641 184 2007 Fragments arranged by size: 2641 2007 1571 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4581 Fragments arranged by size: 4581 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2349 3554 4361 4720 4879 518 Size: 648 1183 1205 807 359 159 2042 Fragments arranged by size: 2042 1205 1183 807 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2509 2645 3153 3467 3819 4496 5644 5889 Size: 903 136 508 314 352 677 1148 245 Cuts at: 5889 1606 Size: 2120 Fragments arranged by size: 2120 1148 903 677 508 352 314 245 136 NaeI GCC'GGC Cuts at: 2418 2418 Size: 6403 NcoI C'CATG_G Cuts at: 513 1065 2004 2787 513 Size: 552 939 783 4129 Fragments arranged by size: 4129 939 783 552 NdeI CA'TA_TG Cuts at: 387 2721 387 Size: 2334 4069 NgoMIV G'CCGG_C Cuts at: 2416 2416 Size: 6403 NheI G'CTAG_C Cuts at: 1052 2743 1052 Size: 1691 4712 NotI GC'GGCC_GC Cuts at: 3850 3850 Size: 6403 NspI r_CATG'y Cuts at: 1132 1229 3761 5306 1132 Size: 97 2532 1545 2229 Fragments arranged by size: 2532 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3631 1348 Size: 2283 4120 PciI A'CATG_T Cuts at: 5302 5302 Size: 6403 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6403 PfoI T'CCnGG_A Cuts at: 3111 3111 Size: 6403 PmeI GTTT'AAAC Cuts at: 3707 3707 Size: 6403 PmlI CAC'GTG Cuts at: 3601 3601 Size: 6403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4472 4504 5985 6017 4472 Size: 32 1481 32 4858 Fragments arranged by size: 4858 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2689 2689 Size: 6403 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6403 PsiI TTA'TAA Cuts at: 3973 3973 Size: 6403 PspXI vC'TCGA_Gb Cuts at: 2758 2758 Size: 6403 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2030 2062 2030 Size: 32 6371 PstI C_TGCA'G Cuts at: 830 2715 3755 830 Size: 1885 1040 3478 Fragments arranged by size: 3478 1885 1040 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6403 PvuII CAG'CTG Cuts at: 2509 3467 2509 Size: 958 5445 SalI G'TCGA_C Cuts at: 1936 3745 1936 Size: 1809 4594 SbfI CC_TGCA'GG Cuts at: 3755 3755 Size: 6403 ScaI AGT'ACT Cuts at: 4651 6363 4651 Size: 1712 4691 SfcI C'TryA_G Cuts at: 826 1045 2711 3751 4886 5567 5758 826 Size: 219 1666 1040 1135 681 191 1471 Fragments arranged by size: 1666 1471 1135 1040 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6403 SmaI CCC'GGG Cuts at: 1872 3730 1872 Size: 1858 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 2758 4507 5408 5670 820 Size: 197 926 486 329 1749 901 262 1553 Fragments arranged by size: 1749 1553 926 901 486 329 262 197 SnaBI TAC'GTA Cuts at: 493 4194 493 Size: 3701 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6403 SphI G_CATG'C Cuts at: 1132 3761 1132 Size: 2629 3774 SspI AAT'ATT Cuts at: 5 52 4327 5 Size: 47 4275 2081 Fragments arranged by size: 4275 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6403 StyI C'CwwG_G Cuts at: 513 1065 2004 2787 3279 3842 513 Size: 552 939 783 492 563 3074 Fragments arranged by size: 3074 939 783 563 552 492 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 4430 4447 4600 4785 5230 325 Size: 1255 2043 807 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4649 6361 96 Size: 274 80 33 51 1443 2672 1712 138 Fragments arranged by size: 2672 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2067 2099 2437 2469 3059 3091 3619 3651 6363 Size: 32 338 32 590 32 528 32 2712 Cuts at: 6363 6395 2067 Size: 32 2075 Fragments arranged by size: 2712 2075 590 528 338 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6403 XbaI T'CTAG_A Cuts at: 2314 3739 2314 Size: 1425 4978 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2231 3109 2231 Size: 878 5525 XhoI C'TCGA_G Cuts at: 1943 2758 1943 Size: 815 5588 XmaI C'CCGG_G Cuts at: 1870 3728 1870 Size: 1858 4545 XmnI GAAnn'nnTTC Cuts at: 4532 4532 Size: 6403 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4211 276 Size: 53 83 186 936 2677 2468 Fragments arranged by size: 2677 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI