################################## # NO CUT ENZYMES ( 167 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BlnI BmyI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MluI MroI MseI MspA1I NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI Tru9I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccII 54 CGCG CG^CG AciI 99 CCGC CCGC(-3/-1) AluI 168 AGCT AG^CT AlwI 254 GGATC GGATC(4/5) AvaII 146 GGTCC G^G[AT]CC BalI 299 TGGCCA TGG^CCA BbsI 31 GTCTTC GAAGAC(2/6) BcnI 26 CCGGG CC^[GC]GG BglI 40 GCCTTCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 539 AGATCT A^GATCT BlpI 343 GCTGAGC GC^T[ACGT]AGC BpmI 594 CTCCAG CTGGAG(16/14) Bpu1102I 343 GCTGAGC GC^T[ACGT]AGC BpuAI 31 GTCTTC GAAGAC(2/6) BsaBI 525 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 127 GAGAC GTCTC(1/5) BsrFI 214 ACCGGC [GA]^CCGG[CT] BssHII 53 GCGCGC G^CGCGC BstUI 54 CGCG CG^CG BstXI 263 CCAAGAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 539 AGATCT [GA]^GATC[CT] CelII 343 GCTGAGC GC^T[ACGT]AGC Cfr10I 214 ACCGGC [GA]^CCGG[CT] DraII 245 AGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 62 CCGTGG C^C[GA][CT]GG EarI 445 CTCTTC CTCTTC(1/4) EcoO109I 245 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoT14I 368 CCAAGG C^C[AT][AT]GG HaeII 550 AGCGCC [GA]GCGC^[CT] Ksp632I 445 CTCTTC CTCTTC(1/4) MaeIII 611 GTGAC ^GT[ACGT]AC MamI 525 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 323 CAATTG C^AATTG MflI 539 AGATCT [GA]^GATC[CT] MluNI 299 TGGCCA TGG^CCA MscI 299 TGGCCA TGG^CCA MslI 312 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 323 CAATTG C^AATTG MvnI 54 CGCG CG^CG NciI 26 CCGGG CC^[GC]GG NheI 9 GCTAGC G^CTAGC SexAI 269 ACCTGGT A^CC[AT]GGT SfiI 39 GGCCTTCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StuI 432 AGGCCT AGG^CCT StyI 368 CCAAGG C^C[AT][AT]GG TfiI 525 GATTC G^A[AT]TC Tsp45I 611 GTGAC ^GT[GC]AC XhoII 539 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BfaI 10 CTAG C^TAG 166 CTAG BseRI 219 CTCCTC GAGGAG(10/8) 236 GAGGAG DdeI 344 CTGAG C^T[ACGT]AG 500 CTCAG DpnII 255 GATC ^GATC 540 GATC HapII 26 CCGG C^CGG 215 CCGG HpaII 26 CCGG C^CGG 215 CCGG HphI 610 GGTGA GGTGA(8/7) 425 TCACC MaeI 10 CTAG C^TAG 166 CTAG MboI 255 GATC ^GATC 540 GATC MspI 26 CCGG C^CGG 215 CCGG NdeII 255 GATC ^GATC 540 GATC PleI 68 GAGTC GAGTC(4/5) 455 GAGTC Sau3AI 255 GATC ^GATC 540 GATC Tsp509I 324 AATT ^AATT 582 AATT XcmI 80 CCATTTTTGATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 596 CCAGAGTGAAGATGG ######################################### # 3 OR MORE CUT ENZYMES ( 28 enzymes) # ######################################### BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I CfoI Cfr13I EaeI EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI