Restriction map for
pFN21AE6275
(Circular) (Six-base) MAPSORT of: pFN21AE6275.seq Check: 6907 from: 1 to: 5320
pFN21AE6275 5320 bp
With 182 enzymes: SgfI *
March 18, 2015 14:50 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3130 278
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2663 1937
Size: 726 4594
Acc65I G'GTAC_C
Cuts at: 2641 2641
Size: 5320
AclI AA'CG_TT
Cuts at: 1563 3447 3820 1563
Size: 1884 373 3063
Fragments arranged by size:
3063 1884 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2576 3383 4767 5035 1632
Size: 944 807 1384 268 1917
Fragments arranged by size:
1917 1384 944 807 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5123
AflIII A'CryG_T
Cuts at: 1203 4219 5196 1203
Size: 3016 977 1327
Fragments arranged by size:
3016 1327 977
AgeI A'CCGG_T
Cuts at: 4151 4151
Size: 5320
AhdI GACnn_n'nnGTC
Cuts at: 4049 4049
Size: 5320
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5320
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5320
AlwNI CAG_nnn'CTG
Cuts at: 3063 4635 3063
Size: 1572 3748
ApaLI G'TGCA_C
Cuts at: 3377 4533 3377
Size: 1156 4164
ApoI r'AATT_y
Cuts at: 1493 2629 2855 3007 5116 1493
Size: 1136 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3874 160
Size: 872 2842 1606
Fragments arranged by size:
2842 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
AvaI C'yCGr_G
Cuts at: 1870 1943 2645 1870
Size: 73 702 4545
Fragments arranged by size:
4545 702 73
BamHI G'GATC_C
Cuts at: 2650 3017 2650
Size: 367 4953
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2641 4096 618
Size: 325 217 627 854 1455 1842
Fragments arranged by size:
1842 1455 854 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2639 1391
Size: 1248 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2029 928
Size: 971 130 4219
Fragments arranged by size:
4219 971 130
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3511 3545 1903
Size: 34 1574 34 3678
Fragments arranged by size:
3678 1574 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3212 4422 3212
Size: 1210 4110
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5320
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2051 3931 136
Size: 107 122 71 631 605 379 1880 1525
Fragments arranged by size:
1880 1525 631 605 379 122 107 71
BglII A'GATC_T
Cuts at: 2544 5315 2544
Size: 2771 2549
BlpI GC'TnA_GC
Cuts at: 2349 2737 2349
Size: 388 4932
Bme1580I G_kGCm'C
Cuts at: 1790 3381 4537 1790
Size: 1591 1156 2573
Fragments arranged by size:
2573 1591 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5320
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3051 4009 442
Size: 416 53 83 399 24 1634 958 1753
Fragments arranged by size:
1753 1634 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2018 1056
Size: 962 4358
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2584 3980 1239
Size: 1345 1396 2579
Fragments arranged by size:
2579 1396 1345
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3445 4310 4608 3445
Size: 865 298 4157
Fragments arranged by size:
4157 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3983 882
Size: 617 2484 2219
Fragments arranged by size:
2484 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3111 493
Size: 711 1907 2702
Fragments arranged by size:
2702 1907 711
BsaBI GATnn'nnATC
Cuts at: 2534 5314 2534
Size: 2780 2540
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3127 3509 4144 275
Size: 53 83 186 936 1594 382 635 1451
Fragments arranged by size:
1594 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3753 4151 4425 4572 4983 3753
Size: 398 274 147 411 4090
Fragments arranged by size:
4090 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2451 2481 1178
Size: 30 165 30 1048 30 4017
Fragments arranged by size:
4017 1048 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2215 2256 1186
Size: 1029 41 4250
Fragments arranged by size:
4250 1029 41
BseYI C'CCAG_C
Cuts at: 2513 4523 5019 2513
Size: 2010 496 2814
Fragments arranged by size:
2814 2010 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4747
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2770 3531 4559 1540
Size: 275 186 769 761 1028 2301
Fragments arranged by size:
2301 1028 769 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3381 3466 4537 3381
Size: 85 1071 4164
Fragments arranged by size:
4164 1071 85
BsmI GAATG_Cn'
Cuts at: 2831 2924 2831
Size: 93 5227
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2639 3381 3466 4537 1391
Size: 399 849 742 85 1071 2174
Fragments arranged by size:
2174 1071 849 742 399 85
BspEI T'CCGG_A
Cuts at: 4983 4983
Size: 5320
BspHI T'CATG_A
Cuts at: 3209 4939 3209
Size: 1730 3590
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2675 844
Size: 1831 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3207 1394
Size: 429 1384 3507
Fragments arranged by size:
3507 1384 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3815 3989 65
Size: 1183 2567 174 1396
Fragments arranged by size:
2567 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 3964 4151 1700
Size: 110 409 1745 187 2869
Fragments arranged by size:
2869 1745 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5320
BssHII G'CGCG_C
Cuts at: 1925 2058 1925
Size: 133 5187
BssSI C'ACGA_G
Cuts at: 3380 4392 3380
Size: 1012 4308
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5320
BstBI TT'CG_AA
Cuts at: 4124 4124
Size: 5320
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5320
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 2275
Size: 5320
BstYI r'GATC_y
Cuts at: 1972 2544 2650 3017 3402 3419 4871 4957 4969
Size: 572 106 367 385 17 1452 86 12
Cuts at: 4969 5315 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 572 385 367 346 106 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 513
Size: 552 1002 3766
Fragments arranged by size:
3766 1002 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4581
Fragments arranged by size:
4581 683 56
BtsI GCAGTG_nn'
Cuts at: 2018 2488 2836 3630 3650 2018
Size: 470 348 794 20 3688
Fragments arranged by size:
3688 794 470 348 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5320
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5285
DraI TTT'AAA
Cuts at: 2624 2624
Size: 5320
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4327 809
Size: 507 3011 1802
Fragments arranged by size:
3011 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2064 2304 2383
Size: 54 1425 50 275 18 234 240 79
Cuts at: 2383 2496 2767 3656 5285 8
Size: 113 271 889 1629 43
Fragments arranged by size:
1629 1425 889 275 271 240 234 113
79 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2767 1537
Size: 275 955 4090
Fragments arranged by size:
4090 955 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2456 3250 1353
Size: 1103 794 3423
Fragments arranged by size:
3423 1103 794
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3907 4281 4427 387
Size: 1064 138 2318 374 146 1280
Fragments arranged by size:
2318 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2584 3383 3980 4767 5035 1239
Size: 393 944 8 799 597 787 268 1524
Fragments arranged by size:
1524 944 799 787 597 393 268 8
EcoO109I rG'GnC_Cy
Cuts at: 2251 2251
Size: 5320
EcoRI G'AATT_C
Cuts at: 2629 3007 2629
Size: 378 4942
FspI TGC'GCA
Cuts at: 3826 3826
Size: 5320
HaeII r_GCGC'y
Cuts at: 1678 2559 4467 1678
Size: 881 1908 2531
Fragments arranged by size:
2531 1908 881
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3961 3993 4035 4067 1373
Size: 32 135 32 2389 32 42 32 2626
Fragments arranged by size:
2626 2389 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2664 2910 669
Size: 988 281 726 246 3079
Fragments arranged by size:
3079 988 726 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5320
HpaI GTT'AAC
Cuts at: 2910 2910
Size: 5320
KpnI G_GTAC'C
Cuts at: 2645 2645
Size: 5320
MfeI C'AATT_G
Cuts at: 2328 2919 2328
Size: 591 4729
MluI A'CGCG_T
Cuts at: 5196 5196
Size: 5320
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 4434 4618 1305
Size: 873 2256 184 2007
Fragments arranged by size:
2256 2007 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2306 10
Size: 54 1425 343 474 3024
Fragments arranged by size:
3024 1425 474 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2321 3278 3637 3796 518
Size: 648 1155 957 359 159 2042
Fragments arranged by size:
2042 1155 957 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2736 3413 4561 4806 1606
Size: 1130 677 1148 245 2120
Fragments arranged by size:
2120 1148 1130 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4768
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5320
NheI G'CTAG_C
Cuts at: 1052 2014 1052
Size: 962 4358
NotI GC'GGCC_GC
Cuts at: 2767 2767
Size: 5320
NspI r_CATG'y
Cuts at: 1132 1229 2678 4223 1132
Size: 97 1449 1545 2229
Fragments arranged by size:
2229 1545 1449 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5320
PciI A'CATG_T
Cuts at: 4219 4219
Size: 5320
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5320
PmeI GTTT'AAAC
Cuts at: 2624 2624
Size: 5320
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3389 3421 4902 4934 3389
Size: 32 1481 32 3775
Fragments arranged by size:
3775 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5320
PsiI TTA'TAA
Cuts at: 2890 2890
Size: 5320
PstI C_TGCA'G
Cuts at: 830 2672 830
Size: 1842 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5320
SalI G'TCGA_C
Cuts at: 1936 2662 1936
Size: 726 4594
SbfI CC_TGCA'GG
Cuts at: 2672 2672
Size: 5320
ScaI AGT'ACT
Cuts at: 3568 5280 3568
Size: 1712 3608
SexAI A'CCwGG_T
Cuts at: 2274 2274
Size: 5320
SfcI C'TryA_G
Cuts at: 826 1045 2668 3803 4484 4675 826
Size: 219 1623 1135 681 191 1471
Fragments arranged by size:
1623 1471 1135 681 219 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2051 2051
Size: 5320
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5320
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5320
SmaI CCC'GGG
Cuts at: 1872 2647 1872
Size: 775 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3424 4325 4587 820
Size: 197 926 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3111 493
Size: 2618 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5320
SphI G_CATG'C
Cuts at: 1132 2678 1132
Size: 1546 3774
SspI AAT'ATT
Cuts at: 5 52 3244 5
Size: 47 3192 2081
Fragments arranged by size:
3192 2081 47
StuI AGG'CCT
Cuts at: 1517 2439 1517
Size: 922 4398
StyI C'CwwG_G
Cuts at: 513 1065 2373 2759 513
Size: 552 1308 386 3074
Fragments arranged by size:
3074 1308 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2603 3347 3364 3517 3702 4147 325
Size: 1255 1023 744 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1023 744 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3566 5278 96
Size: 274 80 33 51 1443 1589 1712 138
Fragments arranged by size:
1712 1589 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2451 2483 5280 5312 2451
Size: 32 2797 32 2459
Fragments arranged by size:
2797 2459 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5320
XbaI T'CTAG_A
Cuts at: 2656 2656
Size: 5320
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2092 2608 2092
Size: 516 4804
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5320
XmaI C'CCGG_G
Cuts at: 1870 2645 1870
Size: 775 4545
XmnI GAAnn'nnTTC
Cuts at: 3449 3449
Size: 5320
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3128 276
Size: 53 83 186 936 1594 2468
Fragments arranged by size:
2468 1594 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI
SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII
SanDI SapI SfoI SrfI SwaI