(Linear) (Six-base) MAPSORT of: KIEE6275.seq  Check: 4209  from: 1  to: 615



KIEE6275.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     615 
   Size:        571      44
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      24     615 
   Size:         24     591
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     615 
   Size:         46     569
 
BglII A'GATC_T

Cuts at:      0     539     615 
   Size:        539      76
 
BlpI GC'TnA_GC

Cuts at:      0     344     615 
   Size:        344     271
 
BmtI G_CTAG'C

Cuts at:      0      13     615 
   Size:         13     602
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     579     615 
   Size:        579      36
 
BsaBI GATnn'nnATC

Cuts at:      0     529     615 
   Size:        529      86
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     446     476     615 
   Size:        446      30     139

  Fragments arranged by size: 

                 446     139      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     210     251     615 
   Size:        210      41     364

  Fragments arranged by size: 

                 364     210      41
 
BseYI C'CCAG_C

Cuts at:      0     508     615 
   Size:        508     107
 
BsrFI r'CCGG_y

Cuts at:      0     214     615 
   Size:        214     401
 
BssHII G'CGCG_C

Cuts at:      0      53     615 
   Size:         53     562
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     270     615 
   Size:        270     345
 
BstYI r'GATC_y

Cuts at:      0     539     615 
   Size:        539      76
 
BtgI C'CryG_G

Cuts at:      0      62     615 
   Size:         62     553
 
BtsI GCAGTG_nn'

Cuts at:      0      13     483     615 
   Size:         13     470     132

  Fragments arranged by size: 

                 470     132      13
 
EaeI y'GGCC_r

Cuts at:      0      59     299     378     491     615 
   Size:         59     240      79     113     124

  Fragments arranged by size: 

                 240     124     113      79      59
 
EarI CTCTTCn'nnn_

Cuts at:      0     451     615 
   Size:        451     164
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     571     579     615 
   Size:        571       8      36

  Fragments arranged by size: 

                 571      36       8
 
EcoO109I rG'GnC_Cy

Cuts at:      0     246     615 
   Size:        246     369
 
HaeII r_GCGC'y

Cuts at:      0     554     615 
   Size:        554      61
 
MfeI C'AATT_G

Cuts at:      0     323     615 
   Size:        323     292
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     173     615 
   Size:        173     442
 
MscI TGG'CCA

Cuts at:      0     301     615 
   Size:        301     314
 
MslI CAynn'nnrTG

Cuts at:      0     316     615 
   Size:        316     299
 
NheI G'CTAG_C

Cuts at:      0       9     615 
   Size:          9     606
 
SexAI A'CCwGG_T

Cuts at:      0     269     615 
   Size:        269     346
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      46     615 
   Size:         46     569
 
StuI AGG'CCT

Cuts at:      0     434     615 
   Size:        434     181
 
StyI C'CwwG_G

Cuts at:      0     368     615 
   Size:        368     247
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     598     615 
   Size:        598      17
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     446     478     615 
   Size:        446      32     137

  Fragments arranged by size: 

                 446     137      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      87     603     615 
   Size:         87     516      12

  Fragments arranged by size: 

                 516      87      12
 

 Enzymes that do cut:

     AcuI     BbsI     BglI    BglII     BlpI     BmtI     BpmI    BsaBI
    BsaXI    BseRI    BseYI    BsrFI   BssHII    BstXI    BstYI     BtgI
     BtsI     EaeI     EarI  Eco57MI EcoO109I    HaeII     MfeI     MmeI
     MscI     MslI     NheI    SexAI     SfiI     StuI     StyI    TaqII
     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
 Bme1580I    BmgBI     BmrI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MluI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfcI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI