################################## # NO CUT ENZYMES ( 117 enzymes) # ################################## AatII AccI AccII AclI AcsI AcyI AflII AgeI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI AviII BalI BamHI BbeI BcgI BciVI BclI BfrI BglI BlpI Bpu1102I BsaHI BsaI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI EaeI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HpaI KasI KspI MluI MluNI MscI MspA1I MvnI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI TaqI TthHB8I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 67 enzymes) # ################################### Acc65I 174 GGTACC G^GTACC AccIII 644 TCCGGA T^CCGGA AflIII 567 ACATGT A^C[GA][CT]GT AhdI 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 215 AGCGCT AGC^GCT Asp700I 550 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 174 GGTACC G^GTACC AspEI 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1405 GAGCTC G[AT]GC[AT]^C AspI 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 166 CCCGGG C^[CT]CG[GA]G AvrII 846 CCTAGG C^CTAGG BanI 174 GGTACC G^G[CT][GA]CC BbrPI 112 CACGTG CAC^GTG BbsI 55 GTCTTC GAAGAC(2/6) BbvCI 458 CCTCAGC CCTCAGC(-5/-2) BglII 264 AGATCT A^GATCT BlnI 846 CCTAGG C^CTAGG BpmI 687 CTGGAG CTGGAG(16/14) BpuAI 55 GTCTTC GAAGAC(2/6) BsaAI 112 CACGTG [CT]AC^GT[GA] BsaBI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 644 TCCGGA [AT]^CCGG[AT] BseAI 644 TCCGGA T^CCGGA BsiHKAI 1405 GAGCTC G[AT]GC[AT]^C BsoBI 166 CCCGGG C^[CT]CG[GA]G BspEI 644 TCCGGA T^CCGGA BspHI 1197 TCATGA T^CATGA BspLU11I 567 ACATGT A^CATGT BsrFI 100 GCCGGC [GA]^CCGG[CT] BstAPI 91 GCAGGGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1290 CCATGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 100 GCCGGC [GA]^CCGG[CT] DraII 1343 AGGCCCC [GA]G^G[ACGT]CC[CT] DraIII 1064 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1122 GACCAACAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1063 CCACGG C^C[GA][CT]GG Eam1105I 358 GACCAAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 215 AGCGCT AGC^GCT EcoNI 756 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1343 AGGCCCC [GA]G^G[ACGT]CC[CT] HaeII 215 AGCGCT [GA]GCGC^[CT] HindIII 553 AAGCTT A^AGCTT KpnI 174 GGTACC GGTAC^C MamI 259 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1262 CAATTG C^AATTG MroI 644 TCCGGA T^CCGGA MseI 1052 TTAA T^TAA MunI 1262 CAATTG C^AATTG NaeI 100 GCCGGC GCC^GGC NgoMIV 100 GCCGGC G^CCGGC NspI 567 ACATGT [GA]CATG^[CT] PflFI 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 112 CACGTG CAC^GTG PmlI 112 CACGTG CAC^GTG RcaI 1197 TCATGA T^CATGA SacI 1405 GAGCTC GAGCT^C SbfI 420 CCTGCAGG CCTGCA^GG ScaI 857 AGTACT AGT^ACT SmaI 166 CCCGGG CCC^GGG Sse8387I 420 CCTGCAGG CCTGCA^GG Tru9I 1052 TTAA T^TAA Tth111I 995 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 1152 CCACAATTTCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 166 CCCGGG C^CCGGG XmaI 166 CCCGGG C^CCGGG XmnI 550 GAAAAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### BanII 695 GAGCCC G[GA]GC[CT]^C 1405 GAGCTC BspMI 969 ACCTGC ACCTGC(4/8) 423 GCAGGT BstYI 264 AGATCT [GA]^GATC[CT] 335 AGATCC Cac8I 19 GCTGGC GC[ACGT]^[ACGT]GC 100 GCCGGC CfoI 216 GCGC GCG^C 348 GCGC FauI 210 CCCGC CCCGC(4/6) 1032 CCCGC HhaI 216 GCGC GCG^C 348 GCGC HinP1I 216 GCGC G^CGC 348 GCGC MaeII 113 ACGT A^CGT 344 ACGT MflI 264 AGATCT [GA]^GATC[CT] 335 AGATCC MslI 504 CATCATGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1291 CATGCTAATG SapI 539 GAAGAGC GCTCTTC(1/4) 692 GAAGAGC TfiI 915 GATTC G^A[AT]TC 1258 GAATC Tsp509I 1156 AATT ^AATT 1263 AATT XhoII 264 AGATCT [GA]^GATC[CT] 335 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AfaI AluI AlwI AvaII BbvI BcnI BfaI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TseI Tsp45I TspRI