(Circular) (Six-base) MAPSORT of: pFN21AE1936.seq Check: 5008 from: 1 to: 6130 pFN21AE1936 6130 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2983 2983 Size: 6130 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 2179 3451 2179 Size: 1272 4858 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 3380 3389 4193 5577 5845 1632 Size: 1049 699 9 804 1384 268 1917 Fragments arranged by size: 1917 1384 1049 804 699 268 9 AfeI AGC'GCT Cuts at: 2222 2222 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 2572 5029 6006 1203 Size: 1369 2457 977 1327 Fragments arranged by size: 2457 1369 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 2368 4859 2368 Size: 2491 3639 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6130 AlwNI CAG_nnn'CTG Cuts at: 3873 5445 3873 Size: 1572 4558 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2171 3455 1870 Size: 73 228 1284 4545 Fragments arranged by size: 4545 1284 228 73 AvrII C'CTAG_G Cuts at: 2851 2851 Size: 6130 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2179 3451 4906 618 Size: 325 217 627 392 1272 1455 1842 Fragments arranged by size: 1842 1455 1272 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2704 3414 3449 1391 Size: 1313 710 35 4072 Fragments arranged by size: 4072 1313 710 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2053 928 Size: 971 154 5005 Fragments arranged by size: 5005 971 154 BbvCI CC'TCA_GC Cuts at: 2464 2464 Size: 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2269 6125 2269 Size: 3856 2274 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2138 4191 5347 1790 Size: 348 2053 1156 2573 Fragments arranged by size: 2573 2053 1156 348 BmgBI CAC'GTC Cuts at: 1318 2350 1318 Size: 1032 5098 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2896 3861 4819 Size: 416 53 83 399 24 1479 965 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1479 965 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2713 4790 1239 Size: 1474 2077 2579 Fragments arranged by size: 2579 2077 1474 Bpu10I CC'TnA_GC Cuts at: 2464 2464 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2378 3302 3398 4255 5120 5418 2378 Size: 924 96 857 865 298 3090 Fragments arranged by size: 3090 924 865 857 298 96 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2119 3921 493 Size: 711 915 1802 2702 Fragments arranged by size: 2702 1802 915 711 BsaBI GATnn'nnATC Cuts at: 2268 6124 2268 Size: 3856 2274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3937 4319 4954 275 Size: 53 83 186 936 2404 382 635 1451 Fragments arranged by size: 2404 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2649 4563 4961 5235 5382 5793 2649 Size: 1914 398 274 147 411 2986 Fragments arranged by size: 2986 1914 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 1178 Size: 30 165 30 777 30 5098 Fragments arranged by size: 5098 777 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6130 BseYI C'CCAG_C Cuts at: 3397 5333 5829 3397 Size: 1936 496 3698 Fragments arranged by size: 3698 1936 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5557 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3414 4191 4276 5347 3414 Size: 777 85 1071 4197 Fragments arranged by size: 4197 1071 777 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2138 2704 3414 3449 4191 4276 5347 Size: 399 348 566 710 35 742 85 1071 Cuts at: 5347 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 710 566 399 348 85 35 BspEI T'CCGG_A Cuts at: 2649 5793 2649 Size: 3144 2986 BspHI T'CATG_A Cuts at: 3202 4019 5749 3202 Size: 817 1730 3583 Fragments arranged by size: 3583 1730 817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2419 2983 3485 844 Size: 1575 564 502 3489 Fragments arranged by size: 3489 1575 564 502 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 4774 4961 1700 Size: 110 295 2669 187 2869 Fragments arranged by size: 2869 2669 295 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 4190 5202 4190 Size: 1012 5118 BstAPI GCAn_nnn'nTGC Cuts at: 794 2102 794 Size: 1308 4822 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3302 2011 Size: 1291 4839 BstYI r'GATC_y Cuts at: 1972 2269 2340 3460 3827 4212 4229 5681 5767 Size: 297 71 1120 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1120 385 367 346 297 86 71 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3068 513 Size: 552 939 1064 3575 Fragments arranged by size: 3575 1064 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2475 2727 3646 4440 4460 2475 Size: 252 919 794 20 4145 Fragments arranged by size: 4145 919 794 252 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 3074 3074 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3133 5137 809 Size: 507 1817 2004 1802 Fragments arranged by size: 2004 1817 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3577 4466 6095 Size: 54 1425 50 275 18 1747 889 1629 Cuts at: 6095 8 Size: 43 Fragments arranged by size: 1747 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2539 2692 3334 4060 1353 Size: 1186 153 642 726 3423 Fragments arranged by size: 3423 1186 726 642 153 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3041 4717 5091 5237 387 Size: 1064 138 1452 1676 374 146 1280 Fragments arranged by size: 1676 1452 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3412 3412 Size: 6130 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2681 2713 3380 3389 4193 4790 5577 Size: 393 1049 32 667 9 804 597 787 Cuts at: 5577 5845 1239 Size: 268 1524 Fragments arranged by size: 1524 1049 804 787 667 597 393 268 32 9 EcoNI CCTnn'n_nnAGG Cuts at: 2765 2765 Size: 6130 EcoO109I rG'GnC_Cy Cuts at: 3349 3349 Size: 6130 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2576 2576 Size: 6130 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2224 5277 1678 Size: 546 3053 2531 Fragments arranged by size: 3053 2531 546 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2354 2370 2386 2402 4771 Size: 32 135 32 782 16 16 16 2369 Cuts at: 4771 4803 4845 4877 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2369 782 135 42 32 32 32 32 16 16 16 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 2558 748 Size: 1810 4320 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 2183 3455 2183 Size: 1272 4858 MfeI C'AATT_G Cuts at: 3267 3729 3267 Size: 462 5668 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2374 5244 5428 1305 Size: 1069 2870 184 2007 Fragments arranged by size: 2870 2007 1069 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2513 3300 4088 4447 4606 518 Size: 648 843 504 787 788 359 159 2042 Fragments arranged by size: 2042 843 788 787 648 504 359 159 MspA1I CmG'CkG Cuts at: 1606 3546 4223 5371 5616 1606 Size: 1940 677 1148 245 2120 Fragments arranged by size: 2120 1940 1148 677 245 NaeI GCC'GGC Cuts at: 2107 2107 Size: 6130 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NgoMIV G'CCGG_C Cuts at: 2105 2105 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2576 3488 5033 1132 Size: 97 1347 912 1545 2229 Fragments arranged by size: 2229 1545 1347 912 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 2572 5029 2572 Size: 2457 3673 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PfoI T'CCnGG_A Cuts at: 2244 3087 2244 Size: 843 5287 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PmlI CAC'GTG Cuts at: 2119 2119 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2340 2430 2680 3482 830 Size: 1510 90 250 802 3478 Fragments arranged by size: 3478 1510 802 250 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 SacI G_AGCT'C Cuts at: 3414 3414 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 2539 2692 2539 Size: 153 5977 SbfI CC_TGCA'GG Cuts at: 2430 3482 2430 Size: 1052 5078 ScaI AGT'ACT Cuts at: 2864 4378 6090 2864 Size: 1514 1712 2904 Fragments arranged by size: 2904 1712 1514 SfcI C'TryA_G Cuts at: 826 1045 2336 2426 2676 3478 4613 5294 5485 Size: 219 1291 90 250 802 1135 681 191 Cuts at: 5485 826 Size: 1471 Fragments arranged by size: 1471 1291 1135 802 681 250 219 191 90 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2173 3457 1872 Size: 301 1284 4545 Fragments arranged by size: 4545 1284 301 SmlI C'TyrA_G Cuts at: 820 1017 1943 2357 3317 3413 4234 5135 5397 Size: 197 926 414 960 96 821 901 262 Cuts at: 5397 820 Size: 1553 Fragments arranged by size: 1553 960 926 901 821 414 262 197 96 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6130 StyI C'CwwG_G Cuts at: 513 1065 2004 2184 2365 2851 3391 3569 513 Size: 552 939 180 181 486 540 178 3074 Fragments arranged by size: 3074 939 552 540 486 181 180 178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3170 4157 4174 4327 4512 4957 325 Size: 1255 1590 987 17 153 185 445 1498 Fragments arranged by size: 1590 1498 1255 987 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2862 4376 6088 Size: 274 80 33 51 1443 885 1514 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 1712 1514 1443 885 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2180 2212 2731 2763 6090 6122 2180 Size: 32 519 32 3327 32 2188 Fragments arranged by size: 3327 2188 519 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3003 1316 Size: 1687 4443 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3164 3164 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 2171 3455 1870 Size: 301 1284 4545 Fragments arranged by size: 4545 1284 301 XmnI GAAnn'nnTTC Cuts at: 2559 4259 2559 Size: 1700 4430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI