Restriction map for
pFN21AE1936
(Linear) (Six-base) MAPSORT of: KIEE1936.seq Check: 7211 from: 1 to: 1425
KIEE1936.orf
With 182 enzymes: SgfI *
July 15, 2010 18:51 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 1425
Size: 978 447
Acc65I G'GTAC_C
Cuts at: 0 174 1425
Size: 174 1251
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1375 1384 1425
Size: 676 699 9 41
Fragments arranged by size:
699 676 41 9
AfeI AGC'GCT
Cuts at: 0 217 1425
Size: 217 1208
AflIII A'CryG_T
Cuts at: 0 567 1425
Size: 567 858
AhdI GACnn_n'nnGTC
Cuts at: 0 363 1425
Size: 363 1062
AvaI C'yCGr_G
Cuts at: 0 166 1425
Size: 166 1259
AvrII C'CTAG_G
Cuts at: 0 846 1425
Size: 846 579
BanI G'GyrC_C
Cuts at: 0 174 1425
Size: 174 1251
BanII G_rGCy'C
Cuts at: 0 699 1409 1425
Size: 699 710 16
Fragments arranged by size:
710 699 16
BbsI GAAGACnn'nnnn_
Cuts at: 0 48 1425
Size: 48 1377
BbvCI CC'TCA_GC
Cuts at: 0 459 1425
Size: 459 966
BglII A'GATC_T
Cuts at: 0 264 1425
Size: 264 1161
Bme1580I G_kGCm'C
Cuts at: 0 133 1425
Size: 133 1292
BmgBI CAC'GTC
Cuts at: 0 345 1425
Size: 345 1080
BmrI ACTGGGnnnn_n'
Cuts at: 0 891 1425
Size: 891 534
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 708 1425
Size: 708 717
Bpu10I CC'TnA_GC
Cuts at: 0 459 1425
Size: 459 966
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 1297 1393 1425
Size: 373 924 96 32
Fragments arranged by size:
924 373 96 32
BsaAI yAC'GTr
Cuts at: 0 114 1425
Size: 114 1311
BsaBI GATnn'nnATC
Cuts at: 0 263 1425
Size: 263 1162
BsaWI w'CCGG_w
Cuts at: 0 644 1425
Size: 644 781
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1425
Size: 175 30 1220
Fragments arranged by size:
1220 175 30
BseYI C'CCAG_C
Cuts at: 0 1392 1425
Size: 1392 33
BsiHKAI G_wGCw'C
Cuts at: 0 1409 1425
Size: 1409 16
Bsp1286I G_dGCh'C
Cuts at: 0 133 699 1409 1425
Size: 133 566 710 16
Fragments arranged by size:
710 566 133 16
BspEI T'CCGG_A
Cuts at: 0 644 1425
Size: 644 781
BspHI T'CATG_A
Cuts at: 0 1197 1425
Size: 1197 228
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 978 1425
Size: 414 564 447
Fragments arranged by size:
564 447 414
BsrFI r'CCGG_y
Cuts at: 0 100 1425
Size: 100 1325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 97 1425
Size: 97 1328
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1297 1425
Size: 1297 128
BstYI r'GATC_y
Cuts at: 0 264 335 1425
Size: 264 71 1090
Fragments arranged by size:
1090 264 71
BtgI C'CryG_G
Cuts at: 0 1063 1425
Size: 1063 362
BtsI GCAGTG_nn'
Cuts at: 0 470 722 1425
Size: 470 252 703
Fragments arranged by size:
703 470 252
DraIII CAC_nnn'GTG
Cuts at: 0 1069 1425
Size: 1069 356
DrdI GACnn_nn'nnGTC
Cuts at: 0 1128 1425
Size: 1128 297
EarI CTCTTCn'nnn_
Cuts at: 0 534 687 1329 1425
Size: 534 153 642 96
Fragments arranged by size:
642 534 153 96
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1036 1425
Size: 1036 389
EcoICRI GAG'CTC
Cuts at: 0 1407 1425
Size: 1407 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 708 1375 1384 1425
Size: 676 32 667 9 41
Fragments arranged by size:
676 667 41 32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 1425
Size: 760 665
EcoO109I rG'GnC_Cy
Cuts at: 0 1344 1425
Size: 1344 81
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 571 1425
Size: 571 854
HaeII r_GCGC'y
Cuts at: 0 219 1425
Size: 219 1206
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 349 365 381 397 1425
Size: 349 16 16 16 1028
Fragments arranged by size:
1028 349 16 16 16
HindIII A'AGCT_T
Cuts at: 0 553 1425
Size: 553 872
KpnI G_GTAC'C
Cuts at: 0 178 1425
Size: 178 1247
MfeI C'AATT_G
Cuts at: 0 1262 1425
Size: 1262 163
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1425
Size: 369 1056
MslI CAynn'nnrTG
Cuts at: 0 508 1295 1425
Size: 508 787 130
Fragments arranged by size:
787 508 130
NaeI GCC'GGC
Cuts at: 0 102 1425
Size: 102 1323
NgoMIV G'CCGG_C
Cuts at: 0 100 1425
Size: 100 1325
NspI r_CATG'y
Cuts at: 0 571 1425
Size: 571 854
PciI A'CATG_T
Cuts at: 0 567 1425
Size: 567 858
PfoI T'CCnGG_A
Cuts at: 0 239 1082 1425
Size: 239 843 343
Fragments arranged by size:
843 343 239
PmlI CAC'GTG
Cuts at: 0 114 1425
Size: 114 1311
PstI C_TGCA'G
Cuts at: 0 335 425 675 1425
Size: 335 90 250 750
Fragments arranged by size:
750 335 250 90
SacI G_AGCT'C
Cuts at: 0 1409 1425
Size: 1409 16
SapI GCTCTTCn'nnn_
Cuts at: 0 534 687 1425
Size: 534 153 738
Fragments arranged by size:
738 534 153
SbfI CC_TGCA'GG
Cuts at: 0 425 1425
Size: 425 1000
ScaI AGT'ACT
Cuts at: 0 859 1425
Size: 859 566
SfcI C'TryA_G
Cuts at: 0 331 421 671 1425
Size: 331 90 250 754
Fragments arranged by size:
754 331 250 90
SmaI CCC'GGG
Cuts at: 0 168 1425
Size: 168 1257
SmlI C'TyrA_G
Cuts at: 0 352 1312 1408 1425
Size: 352 960 96 17
Fragments arranged by size:
960 352 96 17
StyI C'CwwG_G
Cuts at: 0 179 360 846 1386 1425
Size: 179 181 486 540 39
Fragments arranged by size:
540 486 181 179 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1165 1425
Size: 1165 260
TatI w'GTAC_w
Cuts at: 0 857 1425
Size: 857 568
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 175 207 726 758 1425
Size: 175 32 519 32 667
Fragments arranged by size:
667 519 175 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 998 1425
Size: 998 427
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1159 1425
Size: 1159 266
XmaI C'CCGG_G
Cuts at: 0 166 1425
Size: 166 1259
XmnI GAAnn'nnTTC
Cuts at: 0 554 1425
Size: 554 871
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AhdI AvaI AvrII
BanI BanII BbsI BbvCI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BseYI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrFI BstAPI BstXI
BstYI BtgI BtsI DraIII DrdI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII KpnI
MfeI MmeI MslI NaeI NgoMIV NspI PciI PfoI
PmlI PstI SacI SapI SbfI ScaI SfcI SmaI
SmlI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglI BlpI
BmtI BplI BsaI BsaHI BseRI BsgI BsiEI BsiWI
BsmI BsmBI BsrBI BsrDI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI
EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI
KasI MluI MscI MspA1I NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
XbaI XhoI ZraI