################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanII BbrPI BbsI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII Bst1107I BstAPI BstEII BstF5I BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DpnII DraI DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI KpnI KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MspA1I MunI MvnI NcoI NdeI NdeII NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfaNI SfiI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 178 GTCTAC GT^[AC][GT]AC AflIII 386 ACGTGT A^C[GA][CT]GT AlwNI 153 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG BbeI 263 GGCGCC GGCGC^C BbvCI 438 GCTGAGG CCTCAGC(-5/-2) BcnI 353 CCGGG CC^[GC]GG BfaI 37 CTAG C^TAG BsaI 3 GGTCTC GGTCTC(1/5) BsmAI 4 GTCTC GTCTC(1/5) BsrBI 267 CCGCTC CCGCTC(-3/-3) BsrFI 260 GCCGGC [GA]^CCGG[CT] BssSI 46 CTCGTG CACGAG(-5/-1) BstBI 332 TTCGAA TT^CGAA Cfr10I 260 GCCGGC [GA]^CCGG[CT] DdeI 439 CTGAG C^T[ACGT]AG DraIII 18 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 528 GAAGAG CTCTTC(1/4) HphI 207 GGTGA GGTGA(8/7) KasI 263 GGCGCC G^GCGCC Ksp632I 528 GAAGAG CTCTTC(1/4) MaeI 37 CTAG C^TAG MaeII 386 ACGT A^CGT MboII 528 GAAGA GAAGA(8/7) MslI 201 CATCTGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 260 GCCGGC GCC^GGC NarI 263 GGCGCC GG^CGCC NciI 353 CCGGG CC^[GC]GG NgoMIV 260 GCCGGC G^CCGGC NheI 36 GCTAGC G^CTAGC NspV 332 TTCGAA TT^CGAA PpuMI 305 GGGACCC [GA]G^G[AT]CC[CT] SexAI 19 ACCTGGT A^CC[AT]GGT SfoI 263 GGCGCC GGC^GCC SfuI 332 TTCGAA TT^CGAA SgrAI 259 CGCCGGCG C[GA]^CCGG[CT]G ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 257 GACGCC G[GA]^CG[CT]C 263 GGCGCC AspHI 24 GTGCTC G[AT]GC[AT]^C 291 GTGCTC AvaII 306 GGACC G^G[AT]CC 506 GGTCC BanI 121 GGCACC G^G[CT][GA]CC 263 GGCGCC BmyI 24 GTGCTC G[AGT]GC[ACT]^C 291 GTGCTC BpmI 294 CTCCAG CTGGAG(16/14) 88 CTGGAG BsaHI 257 GACGCC G[GA]^CG[CT]C 263 GGCGCC BsiHKAI 24 GTGCTC G[AT]GC[AT]^C 291 GTGCTC Bsp1286I 24 GTGCTC G[AGT]GC[ACT]^C 291 GTGCTC BsrI 397 ACTGG ACTGG(1/-1) 296 CCAGT DraII 305 GGGACCC [GA]G^G[ACGT]CC[CT] 337 AGGCCCT EcoO109I 305 GGGACCC [GA]G^G[ACGT]CC[CT] 337 AGGCCCT EcoT14I 117 CCAAGG C^C[AT][AT]GG 277 CCAAGG HaeII 263 GGCGCC [GA]GCGC^[CT] 281 GGCGCT Hin1I 257 GACGCC G[GA]^CG[CT]C 263 GGCGCC PstI 150 CTGCAG CTGCA^G 380 CTGCAG StyI 117 CCAAGG C^C[AT][AT]GG 277 CCAAGG TaqI 164 TCGA T^CGA 333 TCGA TspRI 289 CAGTG CA[GC]TG[ACGT][ACGT]^ 297 CAGTG TthHB8I 164 TCGA T^CGA 333 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmFI BssKI BstNI Cac8I CfoI Cfr13I EcoRII FauI Fnu4HI HaeIII HapII HgaI HhaI HinP1I HpaII ItaI MnlI MspI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfcI TseI