Restriction map for
pFN21AE3041
(Linear) (Six-base) MAPSORT of: KIEE3041.seq Check: 8429 from: 1 to: 534
KIEE3041.orf
With 182 enzymes: SgfI *
March 22, 2012 16:05 ..
AccI GT'mk_AC
Cuts at: 0 179 534
Size: 179 355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 534
Size: 368 166
AflIII A'CryG_T
Cuts at: 0 386 534
Size: 386 148
AlwNI CAG_nnn'CTG
Cuts at: 0 158 534
Size: 158 376
BanI G'GyrC_C
Cuts at: 0 121 263 534
Size: 121 142 271
Fragments arranged by size:
271 142 121
BbeI G_GCGC'C
Cuts at: 0 267 534
Size: 267 267
BbvCI CC'TCA_GC
Cuts at: 0 439 534
Size: 439 95
BmgBI CAC'GTC
Cuts at: 0 387 534
Size: 387 147
BmtI G_CTAG'C
Cuts at: 0 40 534
Size: 40 494
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 445 534
Size: 445 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 279 534
Size: 109 170 255
Fragments arranged by size:
255 170 109
Bpu10I CC'TnA_GC
Cuts at: 0 439 534
Size: 439 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 534
Size: 9 525
BsaHI Gr'CG_yC
Cuts at: 0 258 264 534
Size: 258 6 270
Fragments arranged by size:
270 258 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 490 520 534
Size: 490 30 14
Fragments arranged by size:
490 30 14
BsiHKAI G_wGCw'C
Cuts at: 0 28 295 534
Size: 28 267 239
Fragments arranged by size:
267 239 28
Bsp1286I G_dGCh'C
Cuts at: 0 28 295 534
Size: 28 267 239
Fragments arranged by size:
267 239 28
BsrBI CCG'CTC
Cuts at: 0 269 534
Size: 269 265
BsrFI r'CCGG_y
Cuts at: 0 260 534
Size: 260 274
BssSI C'ACGA_G
Cuts at: 0 46 534
Size: 46 488
BstBI TT'CG_AA
Cuts at: 0 333 534
Size: 333 201
DraIII CAC_nnn'GTG
Cuts at: 0 23 534
Size: 23 511
EarI CTCTTCn'nnn_
Cuts at: 0 523 534
Size: 523 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 279 368 534
Size: 109 170 89 166
Fragments arranged by size:
170 166 109 89
EcoO109I rG'GnC_Cy
Cuts at: 0 306 338 534
Size: 306 32 196
Fragments arranged by size:
306 196 32
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 534
Size: 483 51
HaeII r_GCGC'y
Cuts at: 0 267 285 534
Size: 267 18 249
Fragments arranged by size:
267 249 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 490 522 534
Size: 490 32 12
Fragments arranged by size:
490 32 12
KasI G'GCGC_C
Cuts at: 0 263 534
Size: 263 271
MslI CAynn'nnrTG
Cuts at: 0 205 534
Size: 205 329
NaeI GCC'GGC
Cuts at: 0 262 534
Size: 262 272
NarI GG'CG_CC
Cuts at: 0 264 534
Size: 264 270
NgoMIV G'CCGG_C
Cuts at: 0 260 534
Size: 260 274
NheI G'CTAG_C
Cuts at: 0 36 534
Size: 36 498
PpuMI rG'GwC_Cy
Cuts at: 0 306 534
Size: 306 228
PstI C_TGCA'G
Cuts at: 0 154 384 534
Size: 154 230 150
Fragments arranged by size:
230 154 150
SanDI GG'GwC_CC
Cuts at: 0 306 534
Size: 306 228
SexAI A'CCwGG_T
Cuts at: 0 19 534
Size: 19 515
SfcI C'TryA_G
Cuts at: 0 150 380 416 534
Size: 150 230 36 118
Fragments arranged by size:
230 150 118 36
SfoI GGC'GCC
Cuts at: 0 265 534
Size: 265 269
SgrAI Cr'CCGG_yG
Cuts at: 0 260 534
Size: 260 274
StyI C'CwwG_G
Cuts at: 0 117 277 534
Size: 117 160 257
Fragments arranged by size:
257 160 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 139 195 494 534
Size: 139 56 299 40
Fragments arranged by size:
299 139 56 40
Enzymes that do cut:
AccI AcuI AflIII AlwNI BanI BbeI BbvCI BmgBI
BmtI BplI BpmI Bpu10I BsaI BsaHI BsaXI BsiHKAI
Bsp1286I BsrBI BsrFI BssSI BstBI DraIII EarI Eco57MI
EcoO109I FalI HaeII Hin4I KasI MslI NaeI NarI
NgoMIV NheI PpuMI PstI SanDI SexAI SfcI SfoI
SgrAI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BpuEI BsaAI BsaBI BsaWI BseRI BseYI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI
BssHII BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MspA1I NcoI NdeI NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfiI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI