(Circular) (Six-base) MAPSORT of: pFN21AE4306.seq Check: 6972 from: 1 to: 5623 pFN21AE4306 5623 bp With 182 enzymes: SgfI * March 28, 2012 14:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3433 278 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2966 1937 Size: 1029 4594 Acc65I G'GTAC_C Cuts at: 2944 2944 Size: 5623 AclI AA'CG_TT Cuts at: 1563 3750 4123 1563 Size: 2187 373 3063 Fragments arranged by size: 3063 2187 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2690 2858 2904 3686 5070 5338 1632 Size: 1058 168 46 782 1384 268 1917 Fragments arranged by size: 1917 1384 1058 782 268 168 46 AflII C'TTAA_G Cuts at: 820 1017 2149 820 Size: 197 1132 4294 Fragments arranged by size: 4294 1132 197 AflIII A'CryG_T Cuts at: 1203 4522 5499 1203 Size: 3319 977 1327 Fragments arranged by size: 3319 1327 977 AgeI A'CCGG_T Cuts at: 4454 4454 Size: 5623 AhdI GACnn_n'nnGTC Cuts at: 4352 4352 Size: 5623 AleI CACnn'nnGTG Cuts at: 1166 2481 1166 Size: 1315 4308 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5623 AlwNI CAG_nnn'CTG Cuts at: 2083 3366 4938 2083 Size: 1283 1572 2768 Fragments arranged by size: 2768 1572 1283 ApaLI G'TGCA_C Cuts at: 3680 4836 3680 Size: 1156 4467 ApoI r'AATT_y Cuts at: 1493 2196 2690 2932 3158 3310 5419 1493 Size: 703 494 242 226 152 2109 1697 Fragments arranged by size: 2109 1697 703 494 242 226 152 AseI AT'TA_AT Cuts at: 160 1032 4177 160 Size: 872 3145 1606 Fragments arranged by size: 3145 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 AvaI C'yCGr_G Cuts at: 1870 1943 2067 2948 1870 Size: 73 124 881 4545 Fragments arranged by size: 4545 881 124 73 BamHI G'GATC_C Cuts at: 2308 2953 3320 2308 Size: 645 367 4611 Fragments arranged by size: 4611 645 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2095 2944 4399 618 Size: 325 217 627 308 849 1455 1842 Fragments arranged by size: 1842 1455 849 627 325 308 217 BanII G_rGCy'C Cuts at: 1391 2474 2942 1391 Size: 1083 468 4072 Fragments arranged by size: 4072 1083 468 BbeI G_GCGC'C Cuts at: 2099 2099 Size: 5623 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2231 928 Size: 971 332 4320 Fragments arranged by size: 4320 971 332 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3814 3848 1903 Size: 34 1877 34 3678 Fragments arranged by size: 3678 1877 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3515 4725 3515 Size: 1210 4413 BclI T'GATC_A Cuts at: 1548 2403 1548 Size: 855 4768 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4234 136 Size: 107 122 71 631 605 2562 1525 Fragments arranged by size: 2562 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5618 5618 Size: 5623 BlpI GC'TnA_GC Cuts at: 3040 3040 Size: 5623 Bme1580I G_kGCm'C Cuts at: 1790 3684 4840 1790 Size: 1894 1156 2573 Fragments arranged by size: 2573 1894 1156 BmgBI CAC'GTC Cuts at: 1318 2242 1318 Size: 924 4699 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3354 4312 442 Size: 416 53 83 399 24 1937 958 1753 Fragments arranged by size: 1937 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5623 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4283 1239 Size: 3044 2579 Bpu10I CC'TnA_GC Cuts at: 2304 2304 Size: 5623 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3748 4613 4911 3748 Size: 865 298 4460 Fragments arranged by size: 4460 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2777 4286 882 Size: 617 1278 1509 2219 Fragments arranged by size: 2219 1509 1278 617 BsaAI yAC'GTr Cuts at: 493 1204 3414 493 Size: 711 2210 2702 Fragments arranged by size: 2702 2210 711 BsaBI GATnn'nnATC Cuts at: 2586 2619 5617 2586 Size: 33 2998 2592 Fragments arranged by size: 2998 2592 33 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2096 3430 3812 Size: 53 83 186 936 476 87 1334 382 Cuts at: 3812 4447 275 Size: 635 1451 Fragments arranged by size: 1451 1334 936 635 476 382 186 87 83 53 BsaWI w'CCGG_w Cuts at: 4056 4454 4728 4875 5286 4056 Size: 398 274 147 411 4393 Fragments arranged by size: 4393 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2570 2600 1178 Size: 30 165 30 1167 30 4201 Fragments arranged by size: 4201 1167 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5623 BseYI C'CCAG_C Cuts at: 2510 4826 5322 2510 Size: 2316 496 2811 Fragments arranged by size: 2811 2316 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5050 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3073 3834 4862 1540 Size: 275 186 1072 761 1028 2301 Fragments arranged by size: 2301 1072 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2474 3684 3769 4840 2474 Size: 1210 85 1071 3257 Fragments arranged by size: 3257 1210 1071 85 BsmI GAATG_Cn' Cuts at: 2512 3134 3227 2512 Size: 622 93 4908 Fragments arranged by size: 4908 622 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2474 2942 3684 3769 4840 1391 Size: 399 684 468 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 684 468 399 85 BspEI T'CCGG_A Cuts at: 5286 5286 Size: 5623 BspHI T'CATG_A Cuts at: 3512 5242 3512 Size: 1730 3893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2066 2978 844 Size: 1222 912 3489 Fragments arranged by size: 3489 1222 912 BsrBI CCG'CTC Cuts at: 1394 1823 2629 3510 1394 Size: 429 806 881 3507 Fragments arranged by size: 3507 881 806 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4118 4292 65 Size: 1183 2870 174 1396 Fragments arranged by size: 2870 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 4267 4454 1700 Size: 110 282 2175 187 2869 Fragments arranged by size: 2869 2175 282 187 110 BsrGI T'GTAC_A Cuts at: 96 2793 96 Size: 2697 2926 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5623 BssSI C'ACGA_G Cuts at: 2543 3683 4695 2543 Size: 1140 1012 3471 Fragments arranged by size: 3471 1140 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5623 BstBI TT'CG_AA Cuts at: 4427 4427 Size: 5623 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5623 BstYI r'GATC_y Cuts at: 1972 2308 2953 3320 3705 3722 5174 5260 5272 Size: 336 645 367 385 17 1452 86 12 Cuts at: 5272 5618 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 645 385 367 346 336 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2051 513 Size: 552 939 47 4085 Fragments arranged by size: 4085 939 552 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4884 Fragments arranged by size: 4884 683 56 BtsI GCAGTG_nn' Cuts at: 2379 3139 3933 3953 2379 Size: 760 794 20 4049 Fragments arranged by size: 4049 794 760 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5588 DraI TTT'AAA Cuts at: 2927 2927 Size: 5623 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4630 809 Size: 507 3314 1802 Fragments arranged by size: 3314 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2752 3070 3959 Size: 54 1425 50 275 18 922 318 889 Cuts at: 3959 5588 8 Size: 1629 43 Fragments arranged by size: 1629 1425 922 889 318 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3070 1537 Size: 275 1258 4090 Fragments arranged by size: 4090 1258 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3553 1353 Size: 2200 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4210 4584 4730 387 Size: 1064 138 2621 374 146 1280 Fragments arranged by size: 2621 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2472 2472 Size: 5623 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2690 2858 2904 3686 4283 5070 5338 Size: 393 1058 168 46 782 597 787 268 Cuts at: 5338 1239 Size: 1524 Fragments arranged by size: 1524 1058 787 782 597 393 268 168 46 EcoNI CCTnn'n_nnAGG Cuts at: 2234 2234 Size: 5623 EcoRI G'AATT_C Cuts at: 2932 3310 2932 Size: 378 5245 FspI TGC'GCA Cuts at: 4129 4129 Size: 5623 HaeII r_GCGC'y Cuts at: 1678 2075 2099 4770 1678 Size: 397 24 2671 2531 Fragments arranged by size: 2671 2531 397 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4264 4296 4338 4370 1373 Size: 32 135 32 2692 32 42 32 2626 Fragments arranged by size: 2692 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2967 3213 669 Size: 988 281 1029 246 3079 Fragments arranged by size: 3079 1029 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5623 HpaI GTT'AAC Cuts at: 3213 3213 Size: 5623 KasI G'GCGC_C Cuts at: 2095 2095 Size: 5623 KpnI G_GTAC'C Cuts at: 2948 2948 Size: 5623 MfeI C'AATT_G Cuts at: 3222 3222 Size: 5623 MluI A'CGCG_T Cuts at: 5499 5499 Size: 5623 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4737 4921 1305 Size: 3432 184 2007 Fragments arranged by size: 3432 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2754 10 Size: 54 1425 343 922 2879 Fragments arranged by size: 2879 1425 922 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2436 2481 3581 3940 4099 518 Size: 648 1270 45 1100 359 159 2042 Fragments arranged by size: 2042 1270 1100 648 359 159 45 MspA1I CmG'CkG Cuts at: 1606 2101 2136 3039 3716 4864 5109 1606 Size: 495 35 903 677 1148 245 2120 Fragments arranged by size: 2120 1148 903 677 495 245 35 NaeI GCC'GGC Cuts at: 2094 2094 Size: 5623 NarI GG'CG_CC Cuts at: 2096 2096 Size: 5623 NcoI C'CATG_G Cuts at: 513 1065 2004 2051 513 Size: 552 939 47 4085 Fragments arranged by size: 4085 939 552 47 NdeI CA'TA_TG Cuts at: 387 387 Size: 5623 NgoMIV G'CCGG_C Cuts at: 2092 2092 Size: 5623 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5623 NotI GC'GGCC_GC Cuts at: 3070 3070 Size: 5623 NspI r_CATG'y Cuts at: 1132 1229 2981 4526 1132 Size: 97 1752 1545 2229 Fragments arranged by size: 2229 1752 1545 97 PasI CC'CwG_GG Cuts at: 1348 2350 1348 Size: 1002 4621 PciI A'CATG_T Cuts at: 4522 4522 Size: 5623 PflMI CCAn_nnn'nTGG Cuts at: 1108 2918 1108 Size: 1810 3813 PmeI GTTT'AAAC Cuts at: 2927 2927 Size: 5623 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3692 3724 5205 5237 3692 Size: 32 1481 32 4078 Fragments arranged by size: 4078 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5623 PsiI TTA'TAA Cuts at: 3193 3193 Size: 5623 PstI C_TGCA'G Cuts at: 830 2079 2975 830 Size: 1249 896 3478 Fragments arranged by size: 3478 1249 896 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5623 PvuII CAG'CTG Cuts at: 2136 2136 Size: 5623 SacI G_AGCT'C Cuts at: 2474 2474 Size: 5623 SalI G'TCGA_C Cuts at: 1936 2965 1936 Size: 1029 4594 SbfI CC_TGCA'GG Cuts at: 2975 2975 Size: 5623 ScaI AGT'ACT Cuts at: 3871 5583 3871 Size: 1712 3911 SfcI C'TryA_G Cuts at: 826 1045 2075 2718 2856 2971 4106 4787 4978 Size: 219 1030 643 138 115 1135 681 191 Cuts at: 4978 826 Size: 1471 Fragments arranged by size: 1471 1135 1030 681 643 219 191 138 115 SfoI GGC'GCC Cuts at: 2097 2097 Size: 5623 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5623 SgrAI Cr'CCGG_yG Cuts at: 1700 2092 1700 Size: 392 5231 SmaI CCC'GGG Cuts at: 1872 2950 1872 Size: 1078 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2149 3727 4628 4890 820 Size: 197 926 206 1578 901 262 1553 Fragments arranged by size: 1578 1553 926 901 262 206 197 SnaBI TAC'GTA Cuts at: 493 3414 493 Size: 2921 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5623 SphI G_CATG'C Cuts at: 1132 2981 1132 Size: 1849 3774 SspI AAT'ATT Cuts at: 5 52 3547 5 Size: 47 3495 2081 Fragments arranged by size: 3495 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5623 StyI C'CwwG_G Cuts at: 513 1065 2004 2051 2760 2917 3062 513 Size: 552 939 47 709 157 145 3074 Fragments arranged by size: 3074 939 709 552 157 145 47 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2147 3650 3667 3820 4005 4450 325 Size: 1255 567 1503 17 153 185 445 1498 Fragments arranged by size: 1503 1498 1255 567 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2793 3869 5581 Size: 274 80 33 51 1443 816 1076 1712 Cuts at: 5581 96 Size: 138 Fragments arranged by size: 1712 1443 1076 816 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2829 2861 5583 5615 2829 Size: 32 2722 32 2837 Fragments arranged by size: 2837 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5623 XbaI T'CTAG_A Cuts at: 2959 2959 Size: 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5623 XmaI C'CCGG_G Cuts at: 1870 2948 1870 Size: 1078 4545 XmnI GAAnn'nnTTC Cuts at: 2252 2769 3752 2252 Size: 517 983 4123 Fragments arranged by size: 4123 983 517 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3431 276 Size: 53 83 186 936 1897 2468 Fragments arranged by size: 2468 1897 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI