(Circular) (Six-base) MAPSORT of: pFN21AE2493.seq Check: 6969 from: 1 to: 5494 pFN21AE2493 5494 bp With 182 enzymes: SgfI * January 16, 2014 19:09 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3304 278 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2837 1937 Size: 900 4594 Acc65I G'GTAC_C Cuts at: 2815 2815 Size: 5494 AclI AA'CG_TT Cuts at: 1563 3621 3994 1563 Size: 2058 373 3063 Fragments arranged by size: 3063 2058 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2169 2305 3557 4941 5209 1632 Size: 537 136 1252 1384 268 1917 Fragments arranged by size: 1917 1384 1252 537 268 136 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5297 AflIII A'CryG_T Cuts at: 1203 4393 5370 1203 Size: 3190 977 1327 Fragments arranged by size: 3190 1327 977 AgeI A'CCGG_T Cuts at: 4325 4325 Size: 5494 AhdI GACnn_n'nnGTC Cuts at: 4223 4223 Size: 5494 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5494 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5494 AlwNI CAG_nnn'CTG Cuts at: 2789 3237 4809 2789 Size: 448 1572 3474 Fragments arranged by size: 3474 1572 448 ApaI G_GGCC'C Cuts at: 2478 2478 Size: 5494 ApaLI G'TGCA_C Cuts at: 2599 3551 4707 2599 Size: 952 1156 3386 Fragments arranged by size: 3386 1156 952 ApoI r'AATT_y Cuts at: 1493 2803 3029 3181 5290 1493 Size: 1310 226 152 2109 1697 Fragments arranged by size: 2109 1697 1310 226 152 AseI AT'TA_AT Cuts at: 160 1032 4048 160 Size: 872 3016 1606 Fragments arranged by size: 3016 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 AvaI C'yCGr_G Cuts at: 1870 1943 2393 2819 1870 Size: 73 450 426 4545 Fragments arranged by size: 4545 450 426 73 BamHI G'GATC_C Cuts at: 2824 3191 2824 Size: 367 5127 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2417 2815 4270 618 Size: 325 217 627 630 398 1455 1842 Fragments arranged by size: 1842 1455 630 627 398 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2402 2478 2519 2813 1391 Size: 751 260 76 41 294 4072 Fragments arranged by size: 4072 751 294 260 76 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2731 928 Size: 971 832 3691 Fragments arranged by size: 3691 971 832 BbvCI CC'TCA_GC Cuts at: 2404 2404 Size: 5494 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3685 3719 1903 Size: 34 50 34 1664 34 3678 Fragments arranged by size: 3678 1664 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2243 3386 4596 2243 Size: 1143 1210 3141 Fragments arranged by size: 3141 1210 1143 BclI T'GATC_A Cuts at: 1548 1548 Size: 5494 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2220 4105 136 Size: 107 122 71 631 605 548 1885 1525 Fragments arranged by size: 1885 1525 631 605 548 122 107 71 BglII A'GATC_T Cuts at: 5489 5489 Size: 5494 BlpI GC'TnA_GC Cuts at: 2911 2911 Size: 5494 Bme1580I G_kGCm'C Cuts at: 1790 2478 2603 3555 4711 1790 Size: 688 125 952 1156 2573 Fragments arranged by size: 2573 1156 952 688 125 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5494 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3225 4183 442 Size: 416 53 83 399 24 1808 958 1753 Fragments arranged by size: 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5494 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2161 2419 2161 Size: 258 5236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 4154 1239 Size: 977 1938 2579 Fragments arranged by size: 2579 1938 977 Bpu10I CC'TnA_GC Cuts at: 2404 2404 Size: 5494 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2150 3619 4484 4782 2150 Size: 1469 865 298 2862 Fragments arranged by size: 2862 1469 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2469 2582 4157 882 Size: 617 970 113 1575 2219 Fragments arranged by size: 2219 1575 970 617 113 BsaAI yAC'GTr Cuts at: 493 1204 3285 493 Size: 711 2081 2702 Fragments arranged by size: 2702 2081 711 BsaBI GATnn'nnATC Cuts at: 5488 5488 Size: 5494 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 2259 3301 3683 Size: 53 83 186 936 690 36 1042 382 Cuts at: 3683 4318 275 Size: 635 1451 Fragments arranged by size: 1451 1042 936 690 635 382 186 83 53 36 BsaWI w'CCGG_w Cuts at: 2508 3927 4325 4599 4746 5157 2508 Size: 1419 398 274 147 411 2845 Fragments arranged by size: 2845 1419 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2342 2372 1178 Size: 30 165 30 939 30 4300 Fragments arranged by size: 4300 939 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2392 2537 2623 2653 1186 Size: 1206 145 86 30 4027 Fragments arranged by size: 4027 1206 145 86 30 BseYI C'CCAG_C Cuts at: 4697 5193 4697 Size: 496 4998 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2320 2348 2449 1163 Size: 573 584 28 101 4208 Fragments arranged by size: 4208 584 573 101 28 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2944 3705 4733 1540 Size: 275 186 943 761 1028 2301 Fragments arranged by size: 2301 1028 943 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2142 2174 2307 2603 3555 3640 4711 2142 Size: 32 133 296 952 85 1071 2925 Fragments arranged by size: 2925 1071 952 296 133 85 32 BsmI GAATG_Cn' Cuts at: 2674 3005 3098 2674 Size: 331 93 5070 Fragments arranged by size: 5070 331 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2174 2307 2402 2478 2519 2603 Size: 399 352 32 133 95 76 41 84 Cuts at: 2603 2813 3555 3640 4711 1391 Size: 210 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 352 210 133 95 85 84 76 41 32 BspEI T'CCGG_A Cuts at: 2508 5157 2508 Size: 2649 2845 BspHI T'CATG_A Cuts at: 3383 5113 3383 Size: 1730 3764 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2370 2849 844 Size: 1526 479 3489 Fragments arranged by size: 3489 1526 479 BsrBI CCG'CTC Cuts at: 1394 1823 2527 3381 1394 Size: 429 704 854 3507 Fragments arranged by size: 3507 854 704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2450 3989 4163 65 Size: 1183 1202 1539 174 1396 Fragments arranged by size: 1539 1396 1202 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2658 4138 4325 1700 Size: 110 848 1480 187 2869 Fragments arranged by size: 2869 1480 848 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5494 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5494 BssSI C'ACGA_G Cuts at: 3554 4566 3554 Size: 1012 4482 BstAPI GCAn_nnn'nTGC Cuts at: 794 2292 2789 794 Size: 1498 497 3499 Fragments arranged by size: 3499 1498 497 BstBI TT'CG_AA Cuts at: 4298 4298 Size: 5494 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5494 BstYI r'GATC_y Cuts at: 1972 2824 3191 3576 3593 5045 5131 5143 5489 Size: 852 367 385 17 1452 86 12 346 Cuts at: 5489 1972 Size: 1977 Fragments arranged by size: 1977 1452 852 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2625 2625 Size: 5494 BtgI C'CryG_G Cuts at: 513 1065 2421 2468 513 Size: 552 1356 47 3539 Fragments arranged by size: 3539 1356 552 47 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4755 Fragments arranged by size: 4755 683 56 BtsI GCAGTG_nn' Cuts at: 3010 3804 3824 3010 Size: 794 20 4680 Fragments arranged by size: 4680 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5494 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5459 DraI TTT'AAA Cuts at: 2798 2798 Size: 5494 DraIII CAC_nnn'GTG Cuts at: 2343 2343 Size: 5494 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4501 809 Size: 507 3185 1802 Fragments arranged by size: 3185 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 2941 3830 Size: 54 1425 50 275 18 855 256 889 Cuts at: 3830 5459 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 855 275 256 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2941 1537 Size: 275 1129 4090 Fragments arranged by size: 4090 1129 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2630 3424 1353 Size: 1277 794 3423 Fragments arranged by size: 3423 1277 794 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 2201 4081 4455 4601 387 Size: 1064 138 521 91 1880 374 146 1280 Fragments arranged by size: 1880 1280 1064 521 374 146 138 91 EcoICRI GAG'CTC Cuts at: 2140 2140 Size: 5494 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2169 2216 2305 3557 4154 4941 5209 Size: 393 537 47 89 1252 597 787 268 Cuts at: 5209 1239 Size: 1524 Fragments arranged by size: 1524 1252 787 597 537 393 268 89 47 EcoNI CCTnn'n_nnAGG Cuts at: 2366 2366 Size: 5494 EcoO109I rG'GnC_Cy Cuts at: 2474 2475 2786 2474 Size: 1 311 5182 Fragments arranged by size: 5182 311 1 EcoRI G'AATT_C Cuts at: 2803 3181 2803 Size: 378 5116 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2371 2371 Size: 5494 FspI TGC'GCA Cuts at: 2442 4000 2442 Size: 1558 3936 HaeII r_GCGC'y Cuts at: 1678 4641 1678 Size: 2963 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4135 4167 4209 4241 1373 Size: 32 135 32 2563 32 42 32 2626 Fragments arranged by size: 2626 2563 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2155 2838 3084 669 Size: 988 281 217 683 246 3079 Fragments arranged by size: 3079 988 683 281 246 217 HindIII A'AGCT_T Cuts at: 748 748 Size: 5494 HpaI GTT'AAC Cuts at: 3084 3084 Size: 5494 KpnI G_GTAC'C Cuts at: 2819 2819 Size: 5494 MfeI C'AATT_G Cuts at: 3093 3093 Size: 5494 MluI A'CGCG_T Cuts at: 5370 5370 Size: 5494 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4608 4792 1305 Size: 3303 184 2007 Fragments arranged by size: 3303 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 10 Size: 54 1425 343 855 2817 Fragments arranged by size: 2817 1425 855 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3452 3811 3970 518 Size: 648 2286 359 159 2042 Fragments arranged by size: 2286 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2376 2408 2910 3587 4735 4980 1606 Size: 770 32 502 677 1148 245 2120 Fragments arranged by size: 2120 1148 770 677 502 245 32 NaeI GCC'GGC Cuts at: 2660 2660 Size: 5494 NcoI C'CATG_G Cuts at: 513 1065 2421 513 Size: 552 1356 3586 Fragments arranged by size: 3586 1356 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5494 NgoMIV G'CCGG_C Cuts at: 2658 2658 Size: 5494 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5494 NotI GC'GGCC_GC Cuts at: 2941 2941 Size: 5494 NspI r_CATG'y Cuts at: 1132 1229 2549 2852 4397 1132 Size: 97 1320 303 1545 2229 Fragments arranged by size: 2229 1545 1320 303 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5494 PciI A'CATG_T Cuts at: 4393 4393 Size: 5494 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5494 PmeI GTTT'AAAC Cuts at: 2798 2798 Size: 5494 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3563 3595 5076 5108 3563 Size: 32 1481 32 3949 Fragments arranged by size: 3949 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5494 PsiI TTA'TAA Cuts at: 3064 3064 Size: 5494 PspOMI G'GGCC_C Cuts at: 2474 2474 Size: 5494 PstI C_TGCA'G Cuts at: 830 2785 2846 830 Size: 1955 61 3478 Fragments arranged by size: 3478 1955 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5494 PvuII CAG'CTG Cuts at: 2408 2408 Size: 5494 SacI G_AGCT'C Cuts at: 2142 2142 Size: 5494 SalI G'TCGA_C Cuts at: 1936 2836 1936 Size: 900 4594 SbfI CC_TGCA'GG Cuts at: 2846 2846 Size: 5494 ScaI AGT'ACT Cuts at: 3742 5454 3742 Size: 1712 3782 SfcI C'TryA_G Cuts at: 826 1045 2781 2842 3977 4658 4849 826 Size: 219 1736 61 1135 681 191 1471 Fragments arranged by size: 1736 1471 1135 681 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5494 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5494 SmaI CCC'GGG Cuts at: 1872 2821 1872 Size: 949 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2165 3598 4499 4761 820 Size: 197 926 222 1433 901 262 1553 Fragments arranged by size: 1553 1433 926 901 262 222 197 SnaBI TAC'GTA Cuts at: 493 3285 493 Size: 2792 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5494 SphI G_CATG'C Cuts at: 1132 2852 1132 Size: 1720 3774 SspI AAT'ATT Cuts at: 5 52 3418 5 Size: 47 3366 2081 Fragments arranged by size: 3366 2081 47 StuI AGG'CCT Cuts at: 1517 2120 2315 1517 Size: 603 195 4696 Fragments arranged by size: 4696 603 195 StyI C'CwwG_G Cuts at: 513 1065 2421 2583 2933 513 Size: 552 1356 162 350 3074 Fragments arranged by size: 3074 1356 552 350 162 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2191 3521 3538 3691 3876 4321 325 Size: 1255 611 1330 17 153 185 445 1498 Fragments arranged by size: 1498 1330 1255 611 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3740 5452 96 Size: 274 80 33 51 1443 1763 1712 138 Fragments arranged by size: 1763 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5454 5486 5454 Size: 32 5462 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5494 XbaI T'CTAG_A Cuts at: 2830 2830 Size: 5494 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2419 2680 2419 Size: 261 5233 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5494 XmaI C'CCGG_G Cuts at: 1870 2819 1870 Size: 949 4545 XmnI GAAnn'nnTTC Cuts at: 3623 3623 Size: 5494 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3302 276 Size: 53 83 186 936 1768 2468 Fragments arranged by size: 2468 1768 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI