(Circular) (Six-base) MAPSORT of: pFN21AE2023.seq Check: 8715 from: 1 to: 6502 pFN21AE2023 6502 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4312 278 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3674 3845 1937 Size: 1737 171 4594 Fragments arranged by size: 4594 1737 171 Acc65I G'GTAC_C Cuts at: 3823 3823 Size: 6502 AclI AA'CG_TT Cuts at: 1563 4629 5002 1563 Size: 3066 373 3063 Fragments arranged by size: 3066 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3475 3572 4565 5949 6217 1632 Size: 1843 97 993 1384 268 1917 Fragments arranged by size: 1917 1843 1384 993 268 97 AfeI AGC'GCT Cuts at: 2777 2996 2777 Size: 219 6283 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6305 AflIII A'CryG_T Cuts at: 1203 2647 5401 6378 1203 Size: 1444 2754 977 1327 Fragments arranged by size: 2754 1444 1327 977 AgeI A'CCGG_T Cuts at: 5333 5333 Size: 6502 AhdI GACnn_n'nnGTC Cuts at: 2417 5231 2417 Size: 2814 3688 AleI CACnn'nnGTG Cuts at: 1166 3492 3795 1166 Size: 2326 303 3873 Fragments arranged by size: 3873 2326 303 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3777 1184 Size: 2593 3909 AlwNI CAG_nnn'CTG Cuts at: 2218 3277 3617 3742 3757 4245 5817 2218 Size: 1059 340 125 15 488 1572 2903 Fragments arranged by size: 2903 1572 1059 488 340 125 15 ApaI G_GGCC'C Cuts at: 2862 3154 3255 3392 2862 Size: 292 101 137 5972 Fragments arranged by size: 5972 292 137 101 ApaLI G'TGCA_C Cuts at: 4559 5715 4559 Size: 1156 5346 ApoI r'AATT_y Cuts at: 1493 2066 3811 4037 4189 6298 1493 Size: 573 1745 226 152 2109 1697 Fragments arranged by size: 2109 1745 1697 573 226 152 AseI AT'TA_AT Cuts at: 160 1032 5056 160 Size: 872 4024 1606 Fragments arranged by size: 4024 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 AvaI C'yCGr_G Cuts at: 1870 1943 3316 3396 3537 3827 1870 Size: 73 1373 80 141 290 4545 Fragments arranged by size: 4545 1373 290 141 80 73 BamHI G'GATC_C Cuts at: 3832 4199 3832 Size: 367 6135 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2093 2345 3823 5278 618 Size: 325 217 627 306 252 1478 1455 1842 Fragments arranged by size: 1842 1478 1455 627 325 306 252 217 BanII G_rGCy'C Cuts at: 1391 2862 3015 3049 3154 3255 3392 3403 3438 Size: 1471 153 34 105 101 137 11 35 Cuts at: 3438 3597 3611 3629 3821 1391 Size: 159 14 18 192 4072 Fragments arranged by size: 4072 1471 192 159 153 137 105 101 35 34 18 14 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2458 928 Size: 971 559 4972 Fragments arranged by size: 4972 971 559 BbvCI CC'TCA_GC Cuts at: 2124 2316 2124 Size: 192 6310 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3199 3233 4693 4727 1903 Size: 34 1262 34 1460 34 3678 Fragments arranged by size: 3678 1460 1262 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4394 5604 4394 Size: 1210 5292 BclI T'GATC_A Cuts at: 1548 1548 Size: 6502 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2091 3633 5113 Size: 107 122 71 631 605 419 1542 1480 Cuts at: 5113 136 Size: 1525 Fragments arranged by size: 1542 1525 1480 631 605 419 122 107 71 BglII A'GATC_T Cuts at: 6497 6497 Size: 6502 BlpI GC'TnA_GC Cuts at: 3919 3919 Size: 6502 Bme1580I G_kGCm'C Cuts at: 1790 2096 2580 2862 3154 3255 3392 4563 5719 Size: 306 484 282 292 101 137 1171 1156 Cuts at: 5719 1790 Size: 2573 Fragments arranged by size: 2573 1171 1156 484 306 292 282 137 101 BmgBI CAC'GTC Cuts at: 1318 2575 1318 Size: 1257 5245 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2855 3085 3593 Size: 416 53 83 399 24 1438 230 508 Cuts at: 3593 3789 4233 5191 442 Size: 196 444 958 1753 Fragments arranged by size: 1753 1438 958 508 444 416 399 230 196 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6502 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2149 2485 3790 2149 Size: 336 1305 4861 Fragments arranged by size: 4861 1305 336 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2387 2528 2569 2630 3353 5162 1239 Size: 1148 141 41 61 723 1809 2579 Fragments arranged by size: 2579 1809 1148 723 141 61 41 Bpu10I CC'TnA_GC Cuts at: 2124 2316 2124 Size: 192 6310 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4627 5492 5790 4627 Size: 865 298 5339 Fragments arranged by size: 5339 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2404 5165 882 Size: 617 905 2761 2219 Fragments arranged by size: 2761 2219 905 617 BsaAI yAC'GTr Cuts at: 493 1204 4293 493 Size: 711 3089 2702 Fragments arranged by size: 3089 2702 711 BsaBI GATnn'nnATC Cuts at: 6496 6496 Size: 6502 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2223 4309 4691 5326 Size: 53 83 186 936 690 2086 382 635 Cuts at: 5326 275 Size: 1451 Fragments arranged by size: 2086 1451 936 690 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 2302 4935 5333 5607 5754 6165 2169 Size: 133 2633 398 274 147 411 2506 Fragments arranged by size: 2633 2506 411 398 274 147 133 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6277 Fragments arranged by size: 6277 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2801 3020 3381 3384 1186 Size: 955 660 219 361 3 4304 Fragments arranged by size: 4304 955 660 361 219 3 BseYI C'CCAG_C Cuts at: 2176 2218 2248 2861 2991 3145 3439 3750 3786 Size: 42 30 613 130 154 294 311 36 Cuts at: 3786 5705 6201 2176 Size: 1919 496 2477 Fragments arranged by size: 2477 1919 613 496 311 294 154 130 42 36 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3145 3623 1163 Size: 573 1409 478 4042 Fragments arranged by size: 4042 1409 573 478 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3952 4713 5741 1540 Size: 275 186 1951 761 1028 2301 Fragments arranged by size: 2301 1951 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2907 3015 3438 4563 4648 5719 2907 Size: 108 423 1125 85 1071 3690 Fragments arranged by size: 3690 1125 1071 423 108 85 BsiWI C'GTAC_G Cuts at: 3291 3291 Size: 6502 BsmI GAATG_Cn' Cuts at: 4013 4106 4013 Size: 93 6409 BsmBI CGTCTCn'nnnn_ Cuts at: 3301 3301 Size: 6502 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2096 2580 2862 2907 3015 3049 3154 Size: 399 306 484 282 45 108 34 105 Cuts at: 3154 3255 3392 3403 3438 3597 3611 3629 3821 Size: 101 137 11 35 159 14 18 192 Cuts at: 3821 4563 4648 5719 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 484 399 306 282 192 159 137 108 105 101 85 45 35 34 18 14 11 BspEI T'CCGG_A Cuts at: 2169 6165 2169 Size: 3996 2506 BspHI T'CATG_A Cuts at: 4391 6121 4391 Size: 1730 4772 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3857 844 Size: 3013 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2284 2512 2761 2791 2977 4389 1394 Size: 429 461 228 249 30 186 1412 3507 Fragments arranged by size: 3507 1412 461 429 249 228 186 30 BsrDI GCAATG_nn' Cuts at: 65 1248 4997 5171 65 Size: 1183 3749 174 1396 Fragments arranged by size: 3749 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2850 3069 5146 5333 1700 Size: 110 1040 219 2077 187 2869 Fragments arranged by size: 2869 2077 1040 219 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6502 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6502 BssSI C'ACGA_G Cuts at: 4562 5574 4562 Size: 1012 5490 BstAPI GCAn_nnn'nTGC Cuts at: 794 2599 794 Size: 1805 4697 BstBI TT'CG_AA Cuts at: 5306 5306 Size: 6502 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6502 BstYI r'GATC_y Cuts at: 1972 2196 2622 3428 3768 3832 4199 4584 4601 Size: 224 426 806 340 64 367 385 17 Cuts at: 4601 6053 6139 6151 6497 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 806 426 385 367 346 340 224 86 64 17 12 BstZ17I GTA'TAC Cuts at: 3675 3675 Size: 6502 Bsu36I CC'TnA_GG Cuts at: 2352 2352 Size: 6502 BtgI C'CryG_G Cuts at: 513 1065 2190 2333 2449 3474 3629 513 Size: 552 1125 143 116 1025 155 3386 Fragments arranged by size: 3386 1125 1025 552 155 143 116 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5763 Fragments arranged by size: 5763 683 56 BtsI GCAGTG_nn' Cuts at: 2499 3128 3241 3563 4018 4812 4832 2499 Size: 629 113 322 455 794 20 4169 Fragments arranged by size: 4169 794 629 455 322 113 20 ClaI AT'CG_AT Cuts at: 1554 2424 1554 Size: 870 5632 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2880 2915 529 Size: 35 2316 35 4116 Fragments arranged by size: 4116 2316 35 35 DraI TTT'AAA Cuts at: 3806 3806 Size: 6502 DraIII CAC_nnn'GTG Cuts at: 3709 3709 Size: 6502 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5509 809 Size: 507 4193 1802 Fragments arranged by size: 4193 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2118 2439 2978 Size: 54 1425 50 275 18 288 321 539 Cuts at: 2978 3067 3466 3484 3651 3949 4838 6467 8 Size: 89 399 18 167 298 889 1629 43 Fragments arranged by size: 1629 1425 889 539 399 321 298 288 275 167 89 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3949 1537 Size: 275 2137 4090 Fragments arranged by size: 4090 2137 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2379 4432 1353 Size: 1026 2053 3423 Fragments arranged by size: 3423 2053 1026 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5089 5463 5609 387 Size: 1064 138 3500 374 146 1280 Fragments arranged by size: 3500 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3013 3436 3013 Size: 423 6079 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2387 2528 2569 2630 3353 3475 3572 Size: 393 755 141 41 61 723 122 97 Cuts at: 3572 4565 5162 5949 6217 1239 Size: 993 597 787 268 1524 Fragments arranged by size: 1524 993 787 755 723 597 393 268 141 122 97 61 41 EcoNI CCTnn'n_nnAGG Cuts at: 2929 3653 3710 2929 Size: 724 57 5721 Fragments arranged by size: 5721 724 57 EcoO109I rG'GnC_Cy Cuts at: 3150 3168 3374 3150 Size: 18 206 6278 Fragments arranged by size: 6278 206 18 EcoRI G'AATT_C Cuts at: 3811 4189 3811 Size: 378 6124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2179 2482 2179 Size: 303 6199 FspI TGC'GCA Cuts at: 5008 5008 Size: 6502 HaeII r_GCGC'y Cuts at: 1678 2293 2779 2998 3730 5649 1678 Size: 615 486 219 732 1919 2531 Fragments arranged by size: 2531 1919 732 615 486 219 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2403 2435 3420 3452 3533 Size: 32 135 32 831 32 985 32 81 Cuts at: 3533 3565 5143 5175 5217 5249 1373 Size: 32 1578 32 42 32 2626 Fragments arranged by size: 2626 1578 985 831 135 81 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3846 4092 669 Size: 988 281 1908 246 3079 Fragments arranged by size: 3079 1908 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6502 HpaI GTT'AAC Cuts at: 4092 4092 Size: 6502 KpnI G_GTAC'C Cuts at: 3827 3827 Size: 6502 MfeI C'AATT_G Cuts at: 4101 4101 Size: 6502 MluI A'CGCG_T Cuts at: 2647 6378 2647 Size: 3731 2771 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2754 3327 5616 5800 1305 Size: 1449 573 2289 184 2007 Fragments arranged by size: 2289 2007 1449 573 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 3486 3653 10 Size: 54 1425 343 609 1045 167 2859 Fragments arranged by size: 2859 1425 1045 609 343 167 54 MslI CAynn'nnrTG Cuts at: 518 1166 2531 3492 3795 4460 4819 4978 518 Size: 648 1365 961 303 665 359 159 2042 Fragments arranged by size: 2042 1365 961 665 648 359 303 159 MspA1I CmG'CkG Cuts at: 1606 2123 2335 3757 3918 4595 5743 5988 1606 Size: 517 212 1422 161 677 1148 245 2120 Fragments arranged by size: 2120 1422 1148 677 517 245 212 161 NaeI GCC'GGC Cuts at: 2852 3071 2852 Size: 219 6283 NcoI C'CATG_G Cuts at: 513 1065 2449 3474 513 Size: 552 1384 1025 3541 Fragments arranged by size: 3541 1384 1025 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6502 NgoMIV G'CCGG_C Cuts at: 2850 3069 2850 Size: 219 6283 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6502 NotI GC'GGCC_GC Cuts at: 3949 3949 Size: 6502 NspI r_CATG'y Cuts at: 1132 1229 3860 5405 1132 Size: 97 2631 1545 2229 Fragments arranged by size: 2631 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3164 3611 1348 Size: 1816 447 4239 Fragments arranged by size: 4239 1816 447 PciI A'CATG_T Cuts at: 5401 5401 Size: 6502 PflMI CCAn_nnn'nTGG Cuts at: 1108 3261 3617 3757 1108 Size: 2153 356 140 3853 Fragments arranged by size: 3853 2153 356 140 PfoI T'CCnGG_A Cuts at: 2034 2546 3741 2034 Size: 512 1195 4795 Fragments arranged by size: 4795 1195 512 PmeI GTTT'AAAC Cuts at: 3806 3806 Size: 6502 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4571 4603 6084 6116 4571 Size: 32 1481 32 4957 Fragments arranged by size: 4957 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3168 3168 Size: 6502 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6502 PsiI TTA'TAA Cuts at: 4072 4072 Size: 6502 PspOMI G'GGCC_C Cuts at: 2858 3150 3251 3388 2858 Size: 292 101 137 5972 Fragments arranged by size: 5972 292 137 101 PstI C_TGCA'G Cuts at: 830 2151 2214 3854 830 Size: 1321 63 1640 3478 Fragments arranged by size: 3478 1640 1321 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6502 PvuII CAG'CTG Cuts at: 3757 3757 Size: 6502 SacI G_AGCT'C Cuts at: 3015 3438 3015 Size: 423 6079 SacII CC_GC'GG Cuts at: 2336 2336 Size: 6502 SalI G'TCGA_C Cuts at: 1936 3844 1936 Size: 1908 4594 SanDI GG'GwC_CC Cuts at: 3168 3168 Size: 6502 SbfI CC_TGCA'GG Cuts at: 3854 3854 Size: 6502 ScaI AGT'ACT Cuts at: 3545 4750 6462 3545 Size: 1205 1712 3585 Fragments arranged by size: 3585 1712 1205 SexAI A'CCwGG_T Cuts at: 3705 3705 Size: 6502 SfcI C'TryA_G Cuts at: 826 1045 2147 2210 3415 3850 4985 5666 5857 Size: 219 1102 63 1205 435 1135 681 191 Cuts at: 5857 826 Size: 1471 Fragments arranged by size: 1471 1205 1135 1102 681 435 219 191 63 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6502 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6502 SmaI CCC'GGG Cuts at: 1872 3829 1872 Size: 1957 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3537 4606 5507 5769 820 Size: 197 926 1594 1069 901 262 1553 Fragments arranged by size: 1594 1553 1069 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4293 493 Size: 3800 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6502 SphI G_CATG'C Cuts at: 1132 3860 1132 Size: 2728 3774 SspI AAT'ATT Cuts at: 5 52 4426 5 Size: 47 4374 2081 Fragments arranged by size: 4374 2081 47 StuI AGG'CCT Cuts at: 1517 2132 1517 Size: 615 5887 StyI C'CwwG_G Cuts at: 513 1065 2099 2449 2752 3474 3654 3941 513 Size: 552 1034 350 303 722 180 287 3074 Fragments arranged by size: 3074 1034 722 552 350 303 287 180 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2240 2642 2985 3467 3589 4529 4546 Size: 1255 660 402 343 482 122 940 17 Cuts at: 4546 4699 4884 5329 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 940 660 482 445 402 343 185 153 122 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2802 3543 4748 Size: 274 80 33 51 1443 825 741 1205 Cuts at: 4748 6460 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1205 825 741 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6462 6494 6462 Size: 32 6470 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6502 XbaI T'CTAG_A Cuts at: 3838 3838 Size: 6502 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2245 2245 Size: 6502 XhoI C'TCGA_G Cuts at: 1943 3537 1943 Size: 1594 4908 XmaI C'CCGG_G Cuts at: 1870 3827 1870 Size: 1957 4545 XmnI GAAnn'nnTTC Cuts at: 2777 4631 2777 Size: 1854 4648 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4310 276 Size: 53 83 186 936 2776 2468 Fragments arranged by size: 2776 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BstXI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SfiI SfoI SrfI SwaI