(Linear) (Six-base) MAPSORT of: KIEE2023.seq Check: 2924 from: 1 to: 1797 KIEE2023.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AccI GT'mk_AC Cuts at: 0 1669 1797 Size: 1669 128 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1470 1567 1797 Size: 1470 97 230 Fragments arranged by size: 1470 230 97 AfeI AGC'GCT Cuts at: 0 772 991 1797 Size: 772 219 806 Fragments arranged by size: 806 772 219 AflIII A'CryG_T Cuts at: 0 642 1797 Size: 642 1155 AhdI GACnn_n'nnGTC Cuts at: 0 412 1797 Size: 412 1385 AleI CACnn'nnGTG Cuts at: 0 1487 1790 1797 Size: 1487 303 7 Fragments arranged by size: 1487 303 7 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1772 1797 Size: 1772 25 AlwNI CAG_nnn'CTG Cuts at: 0 213 1272 1612 1737 1752 1797 Size: 213 1059 340 125 15 45 Fragments arranged by size: 1059 340 213 125 45 15 ApaI G_GGCC'C Cuts at: 0 857 1149 1250 1387 1797 Size: 857 292 101 137 410 Fragments arranged by size: 857 410 292 137 101 ApoI r'AATT_y Cuts at: 0 61 1797 Size: 61 1736 AvaI C'yCGr_G Cuts at: 0 1311 1391 1532 1797 Size: 1311 80 141 265 Fragments arranged by size: 1311 265 141 80 BanI G'GyrC_C Cuts at: 0 88 340 1797 Size: 88 252 1457 Fragments arranged by size: 1457 252 88 BanII G_rGCy'C Cuts at: 0 857 1010 1044 1149 1250 1387 1398 1433 Size: 857 153 34 105 101 137 11 35 Cuts at: 1433 1592 1606 1624 1797 Size: 159 14 18 173 Fragments arranged by size: 857 173 159 153 137 105 101 35 34 18 14 11 BbsI GAAGACnn'nnnn_ Cuts at: 0 453 1797 Size: 453 1344 BbvCI CC'TCA_GC Cuts at: 0 119 311 1797 Size: 119 192 1486 Fragments arranged by size: 1486 192 119 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1194 1228 1797 Size: 1194 34 569 Fragments arranged by size: 1194 569 34 BglI GCCn_nnn'nGGC Cuts at: 0 86 1628 1797 Size: 86 1542 169 Fragments arranged by size: 1542 169 86 Bme1580I G_kGCm'C Cuts at: 0 91 575 857 1149 1250 1387 1797 Size: 91 484 282 292 101 137 410 Fragments arranged by size: 484 410 292 282 137 101 91 BmgBI CAC'GTC Cuts at: 0 570 1797 Size: 570 1227 BmrI ACTGGGnnnn_n' Cuts at: 0 850 1080 1588 1784 1797 Size: 850 230 508 196 13 Fragments arranged by size: 850 508 230 196 13 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 144 480 1785 1797 Size: 144 336 1305 12 Fragments arranged by size: 1305 336 144 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 523 564 625 1348 1797 Size: 382 141 41 61 723 449 Fragments arranged by size: 723 449 382 141 61 41 Bpu10I CC'TnA_GC Cuts at: 0 119 311 1797 Size: 119 192 1486 Fragments arranged by size: 1486 192 119 BsaI GGTCTCn'nnnn_ Cuts at: 0 399 1797 Size: 399 1398 BsaHI Gr'CG_yC Cuts at: 0 218 1797 Size: 218 1579 BsaWI w'CCGG_w Cuts at: 0 164 297 1797 Size: 164 133 1500 Fragments arranged by size: 1500 164 133 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 136 796 1015 1376 1379 1797 Size: 136 660 219 361 3 418 Fragments arranged by size: 660 418 361 219 136 3 BseYI C'CCAG_C Cuts at: 0 171 213 243 856 986 1140 1434 1745 Size: 171 42 30 613 130 154 294 311 Cuts at: 1745 1781 1797 Size: 36 16 Fragments arranged by size: 613 311 294 171 154 130 42 36 30 16 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1140 1618 1797 Size: 1140 478 179 Fragments arranged by size: 1140 478 179 BsiHKAI G_wGCw'C Cuts at: 0 902 1010 1433 1797 Size: 902 108 423 364 Fragments arranged by size: 902 423 364 108 BsiWI C'GTAC_G Cuts at: 0 1286 1797 Size: 1286 511 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1296 1797 Size: 1296 501 Bsp1286I G_dGCh'C Cuts at: 0 91 575 857 902 1010 1044 1149 1250 Size: 91 484 282 45 108 34 105 101 Cuts at: 1250 1387 1398 1433 1592 1606 1624 1797 Size: 137 11 35 159 14 18 173 Fragments arranged by size: 484 282 173 159 137 108 105 101 91 45 35 34 18 14 11 BspEI T'CCGG_A Cuts at: 0 164 1797 Size: 164 1633 BsrBI CCG'CTC Cuts at: 0 279 507 756 786 972 1797 Size: 279 228 249 30 186 825 Fragments arranged by size: 825 279 249 228 186 30 BsrFI r'CCGG_y Cuts at: 0 845 1064 1797 Size: 845 219 733 Fragments arranged by size: 845 733 219 BstAPI GCAn_nnn'nTGC Cuts at: 0 594 1797 Size: 594 1203 BstYI r'GATC_y Cuts at: 0 191 617 1423 1763 1797 Size: 191 426 806 340 34 Fragments arranged by size: 806 426 340 191 34 BstZ17I GTA'TAC Cuts at: 0 1670 1797 Size: 1670 127 Bsu36I CC'TnA_GG Cuts at: 0 347 1797 Size: 347 1450 BtgI C'CryG_G Cuts at: 0 185 328 444 1469 1624 1797 Size: 185 143 116 1025 155 173 Fragments arranged by size: 1025 185 173 155 143 116 BtsI GCAGTG_nn' Cuts at: 0 494 1123 1236 1558 1797 Size: 494 629 113 322 239 Fragments arranged by size: 629 494 322 239 113 ClaI AT'CG_AT Cuts at: 0 419 1797 Size: 419 1378 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 875 910 1797 Size: 875 35 887 Fragments arranged by size: 887 875 35 DraIII CAC_nnn'GTG Cuts at: 0 1704 1797 Size: 1704 93 EaeI y'GGCC_r Cuts at: 0 113 434 973 1062 1461 1479 1646 1797 Size: 113 321 539 89 399 18 167 151 Fragments arranged by size: 539 399 321 167 151 113 89 18 EarI CTCTTCn'nnn_ Cuts at: 0 374 1797 Size: 374 1423 EcoICRI GAG'CTC Cuts at: 0 1008 1431 1797 Size: 1008 423 366 Fragments arranged by size: 1008 423 366 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 382 523 564 625 1348 1470 1567 1797 Size: 382 141 41 61 723 122 97 230 Fragments arranged by size: 723 382 230 141 122 97 61 41 EcoNI CCTnn'n_nnAGG Cuts at: 0 924 1648 1705 1797 Size: 924 724 57 92 Fragments arranged by size: 924 724 92 57 EcoO109I rG'GnC_Cy Cuts at: 0 1145 1163 1369 1797 Size: 1145 18 206 428 Fragments arranged by size: 1145 428 206 18 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 174 477 1797 Size: 174 303 1320 Fragments arranged by size: 1320 303 174 HaeII r_GCGC'y Cuts at: 0 288 774 993 1725 1797 Size: 288 486 219 732 72 Fragments arranged by size: 732 486 288 219 72 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 398 430 1415 1447 1528 1560 1797 Size: 398 32 985 32 81 32 237 Fragments arranged by size: 985 398 237 81 32 32 32 MluI A'CGCG_T Cuts at: 0 642 1797 Size: 642 1155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 749 1322 1797 Size: 749 573 475 Fragments arranged by size: 749 573 475 MscI TGG'CCA Cuts at: 0 436 1481 1648 1797 Size: 436 1045 167 149 Fragments arranged by size: 1045 436 167 149 MslI CAynn'nnrTG Cuts at: 0 526 1487 1790 1797 Size: 526 961 303 7 Fragments arranged by size: 961 526 303 7 MspA1I CmG'CkG Cuts at: 0 118 330 1752 1797 Size: 118 212 1422 45 Fragments arranged by size: 1422 212 118 45 NaeI GCC'GGC Cuts at: 0 847 1066 1797 Size: 847 219 731 Fragments arranged by size: 847 731 219 NcoI C'CATG_G Cuts at: 0 444 1469 1797 Size: 444 1025 328 Fragments arranged by size: 1025 444 328 NgoMIV G'CCGG_C Cuts at: 0 845 1064 1797 Size: 845 219 733 Fragments arranged by size: 845 733 219 PasI CC'CwG_GG Cuts at: 0 1159 1606 1797 Size: 1159 447 191 Fragments arranged by size: 1159 447 191 PflMI CCAn_nnn'nTGG Cuts at: 0 1256 1612 1752 1797 Size: 1256 356 140 45 Fragments arranged by size: 1256 356 140 45 PfoI T'CCnGG_A Cuts at: 0 29 541 1736 1797 Size: 29 512 1195 61 Fragments arranged by size: 1195 512 61 29 PpuMI rG'GwC_Cy Cuts at: 0 1163 1797 Size: 1163 634 PspOMI G'GGCC_C Cuts at: 0 853 1145 1246 1383 1797 Size: 853 292 101 137 414 Fragments arranged by size: 853 414 292 137 101 PstI C_TGCA'G Cuts at: 0 146 209 1797 Size: 146 63 1588 Fragments arranged by size: 1588 146 63 PvuII CAG'CTG Cuts at: 0 1752 1797 Size: 1752 45 SacI G_AGCT'C Cuts at: 0 1010 1433 1797 Size: 1010 423 364 Fragments arranged by size: 1010 423 364 SacII CC_GC'GG Cuts at: 0 331 1797 Size: 331 1466 SanDI GG'GwC_CC Cuts at: 0 1163 1797 Size: 1163 634 ScaI AGT'ACT Cuts at: 0 1540 1797 Size: 1540 257 SexAI A'CCwGG_T Cuts at: 0 1700 1797 Size: 1700 97 SfcI C'TryA_G Cuts at: 0 142 205 1410 1797 Size: 142 63 1205 387 Fragments arranged by size: 1205 387 142 63 SmlI C'TyrA_G Cuts at: 0 1532 1797 Size: 1532 265 StuI AGG'CCT Cuts at: 0 127 1797 Size: 127 1670 StyI C'CwwG_G Cuts at: 0 94 444 747 1469 1649 1797 Size: 94 350 303 722 180 148 Fragments arranged by size: 722 350 303 180 148 94 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 235 637 980 1462 1584 1797 Size: 235 402 343 482 122 213 Fragments arranged by size: 482 402 343 235 213 122 TatI w'GTAC_w Cuts at: 0 797 1538 1797 Size: 797 741 259 Fragments arranged by size: 797 741 259 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 240 1797 Size: 240 1557 XhoI C'TCGA_G Cuts at: 0 1532 1797 Size: 1532 265 XmnI GAAnn'nnTTC Cuts at: 0 772 1797 Size: 772 1025 Enzymes that do cut: AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BsrBI BsrFI BstAPI BstYI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I MluI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SanDI ScaI SexAI SfcI SmlI StuI StyI TaqII TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AgeI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaXI BsiEI BsmI BspHI BspMI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BtgZI DraI DrdI EagI EciI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XmaI ZraI