################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BsrGI BsrI BssHII BssSI Bst1107I BstBI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI ClaI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsaI SacI SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcsI 346 GAATTT [GA]^AATT[CT] AlwNI 286 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 346 GAATTT [GA]^AATT[CT] AspI 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 161 TGGCCA TGG^CCA BclI 50 TGATCA T^GATCA BcnI 78 CCGGG CC^[GC]GG BglI 68 GCCTCCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 70 CTCCAG CTGGAG(16/14) BspHI 146 TCATGA T^CATGA BspMI 95 ACCTGC ACCTGC(4/8) BsrBI 25 GAGCGG CCGCTC(-3/-3) BsrDI 230 GCAATG GCAATG(2/0) BsrFI 338 GCCGGT [GA]^CCGG[CT] BstAPI 285 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 81 GGTAACC G^GT[ACGT]ACC BstPI 81 GGTAACC G^GT[ACGT]ACC Cfr10I 338 GCCGGT [GA]^CCGG[CT] CpoI 179 CGGACCG CG^G[AT]CCG DdeI 38 CTGAG C^T[ACGT]AG DpnII 51 GATC ^GATC DraII 65 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 117 CCACGG C^C[GA][CT]GG EaeI 161 TGGCCA [CT]^GGCC[GA] EcoNI 57 CCTACTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 65 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoO65I 81 GGTAACC G^GT[ACGT]ACC EcoT14I 333 CCAAGG C^C[AT][AT]GG FauI 99 GCGGG CCCGC(4/6) FbaI 50 TGATCA T^GATCA HgaI 122 GACGC GACGC(5/10) MboI 51 GATC ^GATC MluNI 161 TGGCCA TGG^CCA MscI 161 TGGCCA TGG^CCA MspA1I 289 CAGCTG C[AC]G^C[GT]G NciI 78 CCGGG CC^[GC]GG NdeII 51 GATC ^GATC NlaIII 147 CATG CATG^ PflFI 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 289 CAGCTG CAG^CTG RcaI 146 TCATGA T^CATGA RsrII 179 CGGACCG CG^G[AT]CCG Sau3AI 51 GATC ^GATC SfiI 67 GGCCTCCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC StyI 333 CCAAGG C^C[AT][AT]GG Tsp509I 347 AATT ^AATT Tth111I 130 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AluI 203 AGCT AG^CT 290 AGCT BsiYI 57 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 72 CCAGGGCCGGG BslI 57 CCTACTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 72 CCAGGGCCGGG BstNI 72 CCAGG CC^[AT]GG 159 CCTGG EcoRII 72 CCAGG ^CC[AT]GG 159 CCTGG HapII 78 CCGG C^CGG 339 CCGG HpaII 78 CCGG C^CGG 339 CCGG MaeII 242 ACGT A^CGT 260 ACGT MspI 78 CCGG C^CGG 339 CCGG MvaI 72 CCAGG CC^[AT]GG 159 CCTGG PstI 61 CTGCAG CTGCA^G 292 CTGCAG SfaNI 139 GATGC GCATC(5/9) 192 GCATC SfcI 61 CTGCAG C^T[GA][CT]AG 292 CTGCAG TaqI 137 TCGA T^CGA 274 TCGA Tsp45I 169 GTCAC ^GT[GC]AC 220 GTGAC TspRI 17 CAGTG CA[GC]TG[ACGT][ACGT]^ 87 CACTG TthHB8I 137 TCGA T^CGA 274 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI AvaII BbvI BsaJI BssKI BstF5I Cfr13I Fnu4HI FokI HaeIII HphI ItaI MaeIII MnlI MwoI Sau96I ScrFI TseI