Restriction map for
pFN21AE3693
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaAI BsaHI BsgI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FauI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MslI MunI MvnI NarI NheI
NotI NruI NsiI NspI PacI
PflFI PmaCI PmeI PmlI PshBI
Psp1406I PstI PvuI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I
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# ONE CUT ENZYMES ( 40 enzymes) #
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AgeI 455 ACCGGT A^CCGGT
AlwNI 713 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1934 CCTCAGG CC^T[ACGT]AGG
ApaI 2765 GGGCCC GGGCC^C
BalI 667 TGGCCA TGG^CCA
BclI 1293 TGATCA T^GATCA
BsaBI 50 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 143 GGTCTC GGTCTC(1/5)
BspHI 2076 TCATGA T^CATGA
BsrBI 321 CCGCTC CCGCTC(-3/-3)
BstAPI 1772 GCAAGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 81 TTCGAA TT^CGAA
BstEII 788 GGTCACC G^GT[ACGT]ACC
BstPI 788 GGTCACC G^GT[ACGT]ACC
BstXI 909 CCATGGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1934 CCTCAGG CC^T[ACGT]AGG
Eco81I 1934 CCTCAGG CC^T[ACGT]AGG
EcoNI 358 CCTCGTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 788 GGTCACC G^GT[ACGT]ACC
FbaI 1293 TGATCA T^GATCA
HaeII 464 GGCGCT [GA]GCGC^[CT]
HindIII 1774 AAGCTT A^AGCTT
MaeII 2166 ACGT A^CGT
MamI 50 GATCTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 667 TGGCCA TGG^CCA
MscI 667 TGGCCA TGG^CCA
MspA1I 2258 CAGCTG C[AC]G^C[GT]G
NaeI 560 GCCGGC GCC^GGC
NdeI 980 CATATG CA^TATG
NgoMIV 560 GCCGGC G^CCGGC
NspV 81 TTCGAA TT^CGAA
PinAI 455 ACCGGT A^CCGGT
PshAI 2233 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2258 CAGCTG CAG^CTG
RcaI 2076 TCATGA T^CATGA
SfcI 237 CTATAG C^T[GA][CT]AG
SfuI 81 TTCGAA TT^CGAA
SmaI 2096 CCCGGG CCC^GGG
XmaCI 2096 CCCGGG C^CCGGG
XmaI 2096 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccIII 1712 TCCGGA T^CCGGA
2738 TCCGGA
AcsI 1351 AAATTT [GA]^AATT[CT]
1557 GAATTT
ApoI 1351 AAATTT [GA]^AATT[CT]
1557 GAATTT
Asp700I 1554 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1670 GAAAGTCTTC
BanI 625 GGCACC G^G[CT][GA]CC
868 GGTGCC
BbvCI 707 CCTCAGC CCTCAGC(-5/-2)
2116 GCTGAGG
BseAI 1712 TCCGGA T^CCGGA
2738 TCCGGA
BspEI 1712 TCCGGA T^CCGGA
2738 TCCGGA
EaeI 562 CGGCCA [CT]^GGCC[GA]
667 TGGCCA
MroI 1712 TCCGGA T^CCGGA
2738 TCCGGA
MseI 1913 TTAA T^TAA
2195 TTAA
PaeR7I 1691 CTCGAG C^TCGAG
2489 CTCGAG
PflMI 765 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCATCCACTGG
SacI 654 GAGCTC GAGCT^C
2486 GAGCTC
SapI 1048 GAAGAGC GCTCTTC(1/4)
35 GCTCTTC
Tru9I 1913 TTAA T^TAA
2195 TTAA
Van91I 765 CCAGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
964 CCATCCACTGG
XbaI 427 TCTAGA T^CTAGA
1044 TCTAGA
XcmI 1748 CCAGTGGCTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2447 CCAAAGACTTTGTGG
XhoI 1691 CTCGAG C^TCGAG
2489 CTCGAG
XmnI 1554 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1670 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbsI BbvI
BcnI BfaI BglII BmyI BpmI
BpuAI BsaJI BsaWI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrFI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspI MvaI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII