Restriction map for
pFN21AE3693
(Circular) (Six-base) MAPSORT of: pFN21AE3693.seq Check: 5622 from: 1 to: 7570
pFN21AE3693 7570 bp
With 182 enzymes: SgfI *
July 6, 2012 17:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5380 278
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4913 1937
Size: 2976 4594
Acc65I G'GTAC_C
Cuts at: 4891 4891
Size: 7570
AclI AA'CG_TT
Cuts at: 1563 5697 6070 1563
Size: 4134 373 3063
Fragments arranged by size:
4134 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3003 4413 5633 7017 7285 1632
Size: 1371 1410 1220 1384 268 1917
Fragments arranged by size:
1917 1410 1384 1371 1220 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7373
AflIII A'CryG_T
Cuts at: 1203 6469 7446 1203
Size: 5266 977 1327
Fragments arranged by size:
5266 1327 977
AgeI A'CCGG_T
Cuts at: 2460 6401 2460
Size: 3941 3629
AhdI GACnn_n'nnGTC
Cuts at: 6299 6299
Size: 7570
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7570
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7570
AlwNI CAG_nnn'CTG
Cuts at: 2723 5313 6885 2723
Size: 2590 1572 3408
Fragments arranged by size:
3408 2590 1572
ApaI G_GGCC'C
Cuts at: 4774 4774
Size: 7570
ApaLI G'TGCA_C
Cuts at: 5627 6783 5627
Size: 1156 6414
ApoI r'AATT_y
Cuts at: 1493 3356 3562 4879 5105 5257 7366 1493
Size: 1863 206 1317 226 152 2109 1697
Fragments arranged by size:
2109 1863 1697 1317 226 206 152
AseI AT'TA_AT
Cuts at: 160 1032 6124 160
Size: 872 5092 1606
Fragments arranged by size:
5092 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
AvaI C'yCGr_G
Cuts at: 1870 1943 2736 3696 4101 4233 4494 4719 4895
Size: 73 793 960 405 132 261 225 176
Cuts at: 4895 1870
Size: 4545
Fragments arranged by size:
4545 960 793 405 261 225 176 132
73
BamHI G'GATC_C
Cuts at: 4900 5267 4900
Size: 367 7203
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2630 2873 4891 6346 618
Size: 325 217 627 843 243 2018 1455 1842
Fragments arranged by size:
2018 1842 1455 843 627 325 243 217
BanII G_rGCy'C
Cuts at: 1391 2663 2858 2951 4495 4774 4889 1391
Size: 1272 195 93 1544 279 115 4072
Fragments arranged by size:
4072 1544 1272 279 195 115 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2445 3068 3672 4815 928
Size: 971 546 623 604 1143 3683
Fragments arranged by size:
3683 1143 971 623 604 546
BbvCI CC'TCA_GC
Cuts at: 2713 4122 2713
Size: 1409 6161
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5761 5795 1903
Size: 34 3824 34 3678
Fragments arranged by size:
3824 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5462 6672 5462
Size: 1210 6360
BclI T'GATC_A
Cuts at: 1548 3298 1548
Size: 1750 5820
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6181 136
Size: 107 122 71 631 605 4509 1525
Fragments arranged by size:
4509 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2054 2066 3790 4195 7565 2054
Size: 12 1724 405 3370 2059
Fragments arranged by size:
3370 2059 1724 405 12
BlpI GC'TnA_GC
Cuts at: 4987 4987
Size: 7570
Bme1580I G_kGCm'C
Cuts at: 1790 3540 4774 5631 6787 1790
Size: 1750 1234 857 1156 2573
Fragments arranged by size:
2573 1750 1234 1156 857
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7570
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3747 4047 4351
Size: 416 53 83 399 24 2330 300 304
Cuts at: 4351 4454 5301 6259 442
Size: 103 847 958 1753
Fragments arranged by size:
2330 1753 958 847 416 399 304 300
103 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3619 3619
Size: 7570
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2646 2753 3423 3494 4225 6230 1239
Size: 915 492 107 670 71 731 2005 2579
Fragments arranged by size:
2579 2005 915 731 670 492 107 71
Bpu10I CC'TnA_GC
Cuts at: 2713 3318 3768 4122 2713
Size: 605 450 354 6161
Fragments arranged by size:
6161 605 450 354
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2035 2622 4234 5695 6560 6858 2035
Size: 587 1612 1461 865 298 2747
Fragments arranged by size:
2747 1612 1461 865 587 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2154 6233 882
Size: 617 655 4079 2219
Fragments arranged by size:
4079 2219 655 617
BsaAI yAC'GTr
Cuts at: 493 1204 5361 493
Size: 711 4157 2702
Fragments arranged by size:
4157 2702 711
BsaBI GATnn'nnATC
Cuts at: 2059 7564 2059
Size: 5505 2065
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5377 5759 6394 275
Size: 53 83 186 936 3844 382 635 1451
Fragments arranged by size:
3844 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2460 3717 4743 6003 6401 6675 6822 7233 2460
Size: 1257 1026 1260 398 274 147 411 2797
Fragments arranged by size:
2797 1260 1257 1026 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2531 2561 3389 3419 3467
Size: 30 165 30 1128 30 828 30 48
Cuts at: 3467 3497 1178
Size: 30 5251
Fragments arranged by size:
5251 1128 828 165 48 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2322 2464 3020 3039 3302 3410 3413 3482
Size: 1136 142 556 19 263 108 3 69
Cuts at: 3482 3491 3494 4333 4336 4427 4494 4619 4722
Size: 9 3 839 3 91 67 125 103
Cuts at: 4722 1186
Size: 4034
Fragments arranged by size:
4034 1136 839 556 263 142 125 108
103 91 69 67 19 9 3 3
3
BseYI C'CCAG_C
Cuts at: 2749 3646 4530 6773 7269 2749
Size: 897 884 2243 496 3050
Fragments arranged by size:
3050 2243 897 884 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6997
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5020 5781 6809 1540
Size: 275 186 3019 761 1028 2301
Fragments arranged by size:
3019 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2663 4421 4495 5631 5716 6787 2663
Size: 1758 74 1136 85 1071 3446
Fragments arranged by size:
3446 1758 1136 1071 85 74
BsmI GAATG_Cn'
Cuts at: 5081 5174 5081
Size: 93 7477
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2663 2858 2951 3540 4421 4495 4774
Size: 399 873 195 93 589 881 74 279
Cuts at: 4774 4889 5631 5716 6787 1391
Size: 115 742 85 1071 2174
Fragments arranged by size:
2174 1071 881 873 742 589 399 279
195 115 93 85 74
BspEI T'CCGG_A
Cuts at: 3717 4743 7233 3717
Size: 1026 2490 4054
Fragments arranged by size:
4054 2490 1026
BspHI T'CATG_A
Cuts at: 4081 5459 7189 4081
Size: 1378 1730 4462
Fragments arranged by size:
4462 1730 1378
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4925 844
Size: 4081 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2328 5457 1394
Size: 429 505 3129 3507
Fragments arranged by size:
3507 3129 505 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6065 6239 65
Size: 1183 4817 174 1396
Fragments arranged by size:
4817 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2346 2460 2565 4731 6214 6401 1700
Size: 110 536 114 105 2166 1483 187 2869
Fragments arranged by size:
2869 2166 1483 536 187 114 110 105
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7570
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7570
BssSI C'ACGA_G
Cuts at: 5630 6642 5630
Size: 1012 6558
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3783 794
Size: 2989 4581
BstBI TT'CG_AA
Cuts at: 2087 6374 2087
Size: 4287 3283
BstEII G'GTnAC_C
Cuts at: 1184 2793 1184
Size: 1609 5961
BstXI CCAn_nnnn'nTGG
Cuts at: 2921 2921
Size: 7570
BstYI r'GATC_y
Cuts at: 1972 2033 2054 2066 2381 3497 3790 4195 4337
Size: 61 21 12 315 1116 293 405 142
Cuts at: 4337 4900 5267 5652 5669 7121 7207 7219 7565
Size: 563 367 385 17 1452 86 12 346
Cuts at: 7565 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 563 405 385 367 346
315 293 142 86 61 21 17 12
12
Bsu36I CC'TnA_GG
Cuts at: 3940 3940
Size: 7570
BtgI C'CryG_G
Cuts at: 513 1065 2263 2288 2669 2914 4422 513
Size: 552 1198 25 381 245 1508 3661
Fragments arranged by size:
3661 1508 1198 552 381 245 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6831
Fragments arranged by size:
6831 683 56
BtsI GCAGTG_nn'
Cuts at: 4681 5086 5880 5900 4681
Size: 405 794 20 6351
Fragments arranged by size:
6351 794 405 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7570
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4411 4446 4768 4803 529
Size: 35 3847 35 322 35 3296
Fragments arranged by size:
3847 3296 322 35 35 35
DraI TTT'AAA
Cuts at: 4874 4874
Size: 7570
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6577 809
Size: 507 5261 1802
Fragments arranged by size:
5261 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2567 2672 5017
Size: 54 1425 50 275 18 737 105 2345
Cuts at: 5017 5906 7535 8
Size: 889 1629 43
Fragments arranged by size:
2345 1629 1425 889 737 275 105 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5017 1537
Size: 275 3205 4090
Fragments arranged by size:
4090 3205 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2557 3048 4354 4377 4389 4519 4596
Size: 694 510 491 1306 23 12 130 77
Cuts at: 4596 4833 5500 1353
Size: 237 667 3423
Fragments arranged by size:
3423 1306 694 667 510 491 237 130
77 23 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4145 6157 6531 6677 387
Size: 1064 138 2556 2012 374 146 1280
Fragments arranged by size:
2556 2012 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2661 4493 2661
Size: 1832 5738
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2646 2753 3003 3423 3494 4225
Size: 393 522 492 107 250 420 71 731
Cuts at: 4225 4413 5633 6230 7017 7285 1239
Size: 188 1220 597 787 268 1524
Fragments arranged by size:
1524 1220 787 731 597 522 492 420
393 268 250 188 107 71
EcoNI CCTnn'n_nnAGG
Cuts at: 2367 2367
Size: 7570
EcoO109I rG'GnC_Cy
Cuts at: 2250 2403 2925 3421 3739 3936 4612 2250
Size: 153 522 496 318 197 676 5208
Fragments arranged by size:
5208 676 522 496 318 197 153
EcoRI G'AATT_C
Cuts at: 4879 5257 4879
Size: 378 7192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2424 3700 4663 2424
Size: 1276 963 5331
Fragments arranged by size:
5331 1276 963
FspI TGC'GCA
Cuts at: 6076 6076
Size: 7570
HaeII r_GCGC'y
Cuts at: 1678 2473 6717 1678
Size: 795 4244 2531
Fragments arranged by size:
4244 2531 795
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2435 2467 2531 2563 3283
Size: 32 135 32 863 32 64 32 720
Cuts at: 3283 3315 3389 3421 3467 3499 3780 3812 6211
Size: 32 74 32 46 32 281 32 2399
Cuts at: 6211 6243 6285 6317 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2399 863 720 281 135 74 64
46 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4914 5160 669
Size: 988 281 2976 246 3079
Fragments arranged by size:
3079 2976 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3779 748
Size: 3031 4539
HpaI GTT'AAC
Cuts at: 5160 5160
Size: 7570
KpnI G_GTAC'C
Cuts at: 4895 4895
Size: 7570
MfeI C'AATT_G
Cuts at: 5169 5169
Size: 7570
MluI A'CGCG_T
Cuts at: 7446 7446
Size: 7570
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2520 6684 6868 1305
Size: 1215 4164 184 2007
Fragments arranged by size:
4164 2007 1215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2674 10
Size: 54 1425 343 842 4906
Fragments arranged by size:
4906 1425 842 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5528 5887 6046 518
Size: 648 4362 359 159 2042
Fragments arranged by size:
4362 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4265 4986 5663 6811 7056 1606
Size: 2659 721 677 1148 245 2120
Fragments arranged by size:
2659 2120 1148 721 677 245
NaeI GCC'GGC
Cuts at: 2567 2567
Size: 7570
NcoI C'CATG_G
Cuts at: 513 1065 2288 2669 2914 513
Size: 552 1223 381 245 5169
Fragments arranged by size:
5169 1223 552 381 245
NdeI CA'TA_TG
Cuts at: 387 2986 387
Size: 2599 4971
NgoMIV G'CCGG_C
Cuts at: 2565 2565
Size: 7570
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7570
NotI GC'GGCC_GC
Cuts at: 5017 5017
Size: 7570
NspI r_CATG'y
Cuts at: 1132 1229 4928 6473 1132
Size: 97 3699 1545 2229
Fragments arranged by size:
3699 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7570
PciI A'CATG_T
Cuts at: 6469 6469
Size: 7570
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2776 2975 1108
Size: 1668 199 5703
Fragments arranged by size:
5703 1668 199
PfoI T'CCnGG_A
Cuts at: 2420 3591 2420
Size: 1171 6399
PmeI GTTT'AAAC
Cuts at: 4874 4874
Size: 7570
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5639 5671 7152 7184 5639
Size: 32 1481 32 6025
Fragments arranged by size:
6025 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2403 2925 3739 3936 4612 2403
Size: 522 814 197 676 5361
Fragments arranged by size:
5361 814 676 522 197
PshAI GACnn'nnGTC
Cuts at: 1613 4242 1613
Size: 2629 4941
PsiI TTA'TAA
Cuts at: 3582 5140 3582
Size: 1558 6012
PspOMI G'GGCC_C
Cuts at: 4770 4770
Size: 7570
PspXI vC'TCGA_Gb
Cuts at: 4494 4494
Size: 7570
PstI C_TGCA'G
Cuts at: 830 4922 830
Size: 4092 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7570
PvuII CAG'CTG
Cuts at: 4265 4265
Size: 7570
SacI G_AGCT'C
Cuts at: 2663 4495 2663
Size: 1832 5738
SalI G'TCGA_C
Cuts at: 1936 4912 1936
Size: 2976 4594
SanDI GG'GwC_CC
Cuts at: 2925 4612 2925
Size: 1687 5883
SapI GCTCTTCn'nnn_
Cuts at: 2047 3048 2047
Size: 1001 6569
SbfI CC_TGCA'GG
Cuts at: 4922 4922
Size: 7570
ScaI AGT'ACT
Cuts at: 5818 7530 5818
Size: 1712 5858
SfcI C'TryA_G
Cuts at: 826 1045 2242 4918 6053 6734 6925 826
Size: 219 1197 2676 1135 681 191 1471
Fragments arranged by size:
2676 1471 1197 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7570
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7570
SmaI CCC'GGG
Cuts at: 1872 4103 4897 1872
Size: 2231 794 4545
Fragments arranged by size:
4545 2231 794
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2050 2637 3696 4249 4494 5674
Size: 197 926 107 587 1059 553 245 1180
Cuts at: 5674 6575 6837 820
Size: 901 262 1553
Fragments arranged by size:
1553 1180 1059 926 901 587 553 262
245 197 107
SnaBI TAC'GTA
Cuts at: 493 5361 493
Size: 4868 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7570
SphI G_CATG'C
Cuts at: 1132 4928 1132
Size: 3796 3774
SspI AAT'ATT
Cuts at: 5 52 5494 5
Size: 47 5442 2081
Fragments arranged by size:
5442 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7570
StyI C'CwwG_G
Cuts at: 513 1065 2288 2414 2607 2669 2899 2914 5009
Size: 552 1223 126 193 62 230 15 2095
Cuts at: 5009 513
Size: 3074
Fragments arranged by size:
3074 2095 1223 552 230 193 126 62
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2085 2211 3143 4642 4675 5597 5614
Size: 1255 505 126 932 1499 33 922 17
Cuts at: 5614 5767 5952 6397 325
Size: 153 185 445 1498
Fragments arranged by size:
1499 1498 1255 932 922 505 445 185
153 126 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4055 4386 4620
Size: 274 80 33 51 1443 2078 331 234
Cuts at: 4620 5816 7528 96
Size: 1196 1712 138
Fragments arranged by size:
2078 1712 1443 1196 331 274 234 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3920 3952 4592 4624 7530 7562 3920
Size: 32 640 32 2906 32 3928
Fragments arranged by size:
3928 2906 640 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7570
XbaI T'CTAG_A
Cuts at: 2432 3049 4906 2432
Size: 617 1857 5096
Fragments arranged by size:
5096 1857 617
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3760 4459 3760
Size: 699 6871
XhoI C'TCGA_G
Cuts at: 1943 3696 4494 1943
Size: 1753 798 5019
Fragments arranged by size:
5019 1753 798
XmaI C'CCGG_G
Cuts at: 1870 4101 4895 1870
Size: 2231 794 4545
Fragments arranged by size:
4545 2231 794
XmnI GAAnn'nnTTC
Cuts at: 3563 3679 5699 3563
Size: 116 2020 5434
Fragments arranged by size:
5434 2020 116
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5378 276
Size: 53 83 186 936 3844 2468
Fragments arranged by size:
3844 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI
NarI NruI NsiI PacI PmlI PsrI RsrII SacII
SexAI SfiI SfoI SrfI SwaI