(Linear) (Six-base) MAPSORT of: KIEE3693.seq Check: 5701 from: 1 to: 2865 KIEE3693.orf With 182 enzymes: SgfI * July 3, 2012 18:14 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 998 2408 2865 Size: 998 1410 457 Fragments arranged by size: 1410 998 457 AgeI A'CCGG_T Cuts at: 0 455 2865 Size: 455 2410 AlwNI CAG_nnn'CTG Cuts at: 0 718 2865 Size: 718 2147 ApaI G_GGCC'C Cuts at: 0 2769 2865 Size: 2769 96 ApoI r'AATT_y Cuts at: 0 1351 1557 2865 Size: 1351 206 1308 Fragments arranged by size: 1351 1308 206 AvaI C'yCGr_G Cuts at: 0 731 1691 2096 2228 2489 2714 2865 Size: 731 960 405 132 261 225 151 Fragments arranged by size: 960 731 405 261 225 151 132 BanI G'GyrC_C Cuts at: 0 625 868 2865 Size: 625 243 1997 Fragments arranged by size: 1997 625 243 BanII G_rGCy'C Cuts at: 0 658 853 946 2490 2769 2865 Size: 658 195 93 1544 279 96 Fragments arranged by size: 1544 658 279 195 96 93 BbsI GAAGACnn'nnnn_ Cuts at: 0 440 1063 1667 2810 2865 Size: 440 623 604 1143 55 Fragments arranged by size: 1143 623 604 440 55 BbvCI CC'TCA_GC Cuts at: 0 708 2117 2865 Size: 708 1409 748 Fragments arranged by size: 1409 748 708 BclI T'GATC_A Cuts at: 0 1293 2865 Size: 1293 1572 BglII A'GATC_T Cuts at: 0 49 61 1785 2190 2865 Size: 49 12 1724 405 675 Fragments arranged by size: 1724 675 405 49 12 Bme1580I G_kGCm'C Cuts at: 0 1535 2769 2865 Size: 1535 1234 96 Fragments arranged by size: 1535 1234 96 BmrI ACTGGGnnnn_n' Cuts at: 0 1742 2042 2346 2449 2865 Size: 1742 300 304 103 416 Fragments arranged by size: 1742 416 304 300 103 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1614 2865 Size: 1614 1251 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 641 748 1418 1489 2220 2865 Size: 149 492 107 670 71 731 645 Fragments arranged by size: 731 670 645 492 149 107 71 Bpu10I CC'TnA_GC Cuts at: 0 708 1313 1763 2117 2865 Size: 708 605 450 354 748 Fragments arranged by size: 748 708 605 450 354 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 30 617 2229 2865 Size: 30 587 1612 636 Fragments arranged by size: 1612 636 587 30 BsaI GGTCTCn'nnnn_ Cuts at: 0 149 2865 Size: 149 2716 BsaBI GATnn'nnATC Cuts at: 0 54 2865 Size: 54 2811 BsaWI w'CCGG_w Cuts at: 0 455 1712 2738 2865 Size: 455 1257 1026 127 Fragments arranged by size: 1257 1026 455 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 526 556 1384 1414 1462 1492 2865 Size: 526 30 828 30 48 30 1373 Fragments arranged by size: 1373 828 526 48 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 317 459 1015 1034 1297 1405 1408 1477 Size: 317 142 556 19 263 108 3 69 Cuts at: 1477 1486 1489 2328 2331 2422 2489 2614 2717 Size: 9 3 839 3 91 67 125 103 Cuts at: 2717 2865 Size: 148 Fragments arranged by size: 839 556 317 263 148 142 125 108 103 91 69 67 19 9 3 3 3 BseYI C'CCAG_C Cuts at: 0 744 1641 2525 2865 Size: 744 897 884 340 Fragments arranged by size: 897 884 744 340 BsiHKAI G_wGCw'C Cuts at: 0 658 2416 2490 2865 Size: 658 1758 74 375 Fragments arranged by size: 1758 658 375 74 Bsp1286I G_dGCh'C Cuts at: 0 658 853 946 1535 2416 2490 2769 2865 Size: 658 195 93 589 881 74 279 96 Fragments arranged by size: 881 658 589 279 195 96 93 74 BspEI T'CCGG_A Cuts at: 0 1712 2738 2865 Size: 1712 1026 127 Fragments arranged by size: 1712 1026 127 BspHI T'CATG_A Cuts at: 0 2076 2865 Size: 2076 789 BsrBI CCG'CTC Cuts at: 0 323 2865 Size: 323 2542 BsrFI r'CCGG_y Cuts at: 0 341 455 560 2726 2865 Size: 341 114 105 2166 139 Fragments arranged by size: 2166 341 139 114 105 BstAPI GCAn_nnn'nTGC Cuts at: 0 1778 2865 Size: 1778 1087 BstBI TT'CG_AA Cuts at: 0 82 2865 Size: 82 2783 BstEII G'GTnAC_C Cuts at: 0 788 2865 Size: 788 2077 BstXI CCAn_nnnn'nTGG Cuts at: 0 916 2865 Size: 916 1949 BstYI r'GATC_y Cuts at: 0 28 49 61 376 1492 1785 2190 2332 Size: 28 21 12 315 1116 293 405 142 Cuts at: 2332 2865 Size: 533 Fragments arranged by size: 1116 533 405 315 293 142 28 21 12 Bsu36I CC'TnA_GG Cuts at: 0 1935 2865 Size: 1935 930 BtgI C'CryG_G Cuts at: 0 258 283 664 909 2417 2865 Size: 258 25 381 245 1508 448 Fragments arranged by size: 1508 448 381 258 245 25 BtsI GCAGTG_nn' Cuts at: 0 2676 2865 Size: 2676 189 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2406 2441 2763 2798 2865 Size: 2406 35 322 35 67 Fragments arranged by size: 2406 322 67 35 35 EaeI y'GGCC_r Cuts at: 0 562 667 2865 Size: 562 105 2198 Fragments arranged by size: 2198 562 105 EarI CTCTTCn'nnn_ Cuts at: 0 42 552 1043 2349 2372 2384 2514 2591 Size: 42 510 491 1306 23 12 130 77 Cuts at: 2591 2828 2865 Size: 237 37 Fragments arranged by size: 1306 510 491 237 130 77 42 37 23 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2140 2865 Size: 2140 725 EcoICRI GAG'CTC Cuts at: 0 656 2488 2865 Size: 656 1832 377 Fragments arranged by size: 1832 656 377 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 149 641 748 998 1418 1489 2220 2408 Size: 149 492 107 250 420 71 731 188 Cuts at: 2408 2865 Size: 457 Fragments arranged by size: 731 492 457 420 250 188 149 107 71 EcoNI CCTnn'n_nnAGG Cuts at: 0 362 2865 Size: 362 2503 EcoO109I rG'GnC_Cy Cuts at: 0 245 398 920 1416 1734 1931 2607 2865 Size: 245 153 522 496 318 197 676 258 Fragments arranged by size: 676 522 496 318 258 245 197 153 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 419 1695 2658 2865 Size: 419 1276 963 207 Fragments arranged by size: 1276 963 419 207 HaeII r_GCGC'y Cuts at: 0 468 2865 Size: 468 2397 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 430 462 526 558 1278 1310 1384 1416 Size: 430 32 64 32 720 32 74 32 Cuts at: 1416 1462 1494 1775 1807 2865 Size: 46 32 281 32 1058 Fragments arranged by size: 1058 720 430 281 74 64 46 32 32 32 32 32 32 HindIII A'AGCT_T Cuts at: 0 1774 2865 Size: 1774 1091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 515 2865 Size: 515 2350 MscI TGG'CCA Cuts at: 0 669 2865 Size: 669 2196 MspA1I CmG'CkG Cuts at: 0 2260 2865 Size: 2260 605 NaeI GCC'GGC Cuts at: 0 562 2865 Size: 562 2303 NcoI C'CATG_G Cuts at: 0 283 664 909 2865 Size: 283 381 245 1956 Fragments arranged by size: 1956 381 283 245 NdeI CA'TA_TG Cuts at: 0 981 2865 Size: 981 1884 NgoMIV G'CCGG_C Cuts at: 0 560 2865 Size: 560 2305 PflMI CCAn_nnn'nTGG Cuts at: 0 771 970 2865 Size: 771 199 1895 Fragments arranged by size: 1895 771 199 PfoI T'CCnGG_A Cuts at: 0 415 1586 2865 Size: 415 1171 1279 Fragments arranged by size: 1279 1171 415 PpuMI rG'GwC_Cy Cuts at: 0 398 920 1734 1931 2607 2865 Size: 398 522 814 197 676 258 Fragments arranged by size: 814 676 522 398 258 197 PshAI GACnn'nnGTC Cuts at: 0 2237 2865 Size: 2237 628 PsiI TTA'TAA Cuts at: 0 1577 2865 Size: 1577 1288 PspOMI G'GGCC_C Cuts at: 0 2765 2865 Size: 2765 100 PspXI vC'TCGA_Gb Cuts at: 0 2489 2865 Size: 2489 376 PvuII CAG'CTG Cuts at: 0 2260 2865 Size: 2260 605 SacI G_AGCT'C Cuts at: 0 658 2490 2865 Size: 658 1832 375 Fragments arranged by size: 1832 658 375 SanDI GG'GwC_CC Cuts at: 0 920 2607 2865 Size: 920 1687 258 Fragments arranged by size: 1687 920 258 SapI GCTCTTCn'nnn_ Cuts at: 0 42 1043 2865 Size: 42 1001 1822 Fragments arranged by size: 1822 1001 42 SfcI C'TryA_G Cuts at: 0 237 2865 Size: 237 2628 SmaI CCC'GGG Cuts at: 0 2098 2865 Size: 2098 767 SmlI C'TyrA_G Cuts at: 0 45 632 1691 2244 2489 2865 Size: 45 587 1059 553 245 376 Fragments arranged by size: 1059 587 553 376 245 45 StyI C'CwwG_G Cuts at: 0 283 409 602 664 894 909 2865 Size: 283 126 193 62 230 15 1956 Fragments arranged by size: 1956 283 230 193 126 62 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 80 206 1138 2637 2670 2865 Size: 80 126 932 1499 33 195 Fragments arranged by size: 1499 932 195 126 80 33 TatI w'GTAC_w Cuts at: 0 2050 2381 2615 2865 Size: 2050 331 234 250 Fragments arranged by size: 2050 331 250 234 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1915 1947 2587 2619 2865 Size: 1915 32 640 32 246 Fragments arranged by size: 1915 640 246 32 32 XbaI T'CTAG_A Cuts at: 0 427 1044 2865 Size: 427 617 1821 Fragments arranged by size: 1821 617 427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1755 2454 2865 Size: 1755 699 411 Fragments arranged by size: 1755 699 411 XhoI C'TCGA_G Cuts at: 0 1691 2489 2865 Size: 1691 798 376 Fragments arranged by size: 1691 798 376 XmaI C'CCGG_G Cuts at: 0 2096 2865 Size: 2096 769 XmnI GAAnn'nnTTC Cuts at: 0 1558 1674 2865 Size: 1558 116 1191 Fragments arranged by size: 1558 1191 116 Enzymes that do cut: AcuI AgeI AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI BclI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV PflMI PfoI PpuMI PshAI PsiI PspOMI PspXI PvuII SacI SanDI SapI SfcI SmaI SmlI StyI TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI BlpI BmgBI BmtI BsaAI BsaHI BsgI BsiEI BsiWI BsmI BsmBI BspMI BsrDI BsrGI BssHII BssSI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MslI NarI NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PsrI PstI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I ZraI