Restriction map for
pFN21AE1294
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# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AflII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AvrII
BamHI BbrPI BbvCI BciVI BclI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspMI BsrDI BsrGI Bst1107I
BstBI BstZ17I Bsu36I CelII ClaI
DraI DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HincII HindII HpaI
KpnI MamI MfeI MluI MroI
MunI NcoI NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI SacI SalI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI SspBI SspI
SwaI TfiI Tsp509I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AclI 1390 AACGTT AA^CGTT
AflIII 1301 ACATGT A^C[GA][CT]GT
AviII 665 TGCGCA TGC^GCA
BcgI 342 CGACGAGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 589 CTAG C^TAG
BglI 1118 GCCGCAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 598 CTGGAG CTGGAG(16/14)
BseRI 592 GAGGAG GAGGAG(10/8)
BsiEI 717 CGACCG CG[GA][CT]^CG
BspLU11I 1301 ACATGT A^CATGT
BssSI 358 CACGAG CACGAG(-5/-1)
BstEII 372 GGTCACC G^GT[ACGT]ACC
BstPI 372 GGTCACC G^GT[ACGT]ACC
CpoI 314 CGGACCG CG^G[AT]CCG
DdeI 43 CTGAG C^T[ACGT]AG
DsaI 1149 CCGCGG C^C[GA][CT]GG
EarI 301 CTCTTC CTCTTC(1/4)
EcoNI 855 CCTGTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 372 GGTCACC G^GT[ACGT]ACC
EcoT14I 1269 CCAAGG C^C[AT][AT]GG
FspI 665 TGCGCA TGC^GCA
HindIII 1234 AAGCTT A^AGCTT
HinfI 985 GACTC G^A[ACGT]TC
Ksp632I 301 CTCTTC CTCTTC(1/4)
KspI 1149 CCGCGG CCGC^GG
MaeI 589 CTAG C^TAG
MaeIII 373 GTCAC ^GT[ACGT]AC
MseI 1220 TTAA T^TAA
NaeI 158 GCCGGC GCC^GGC
NdeI 794 CATATG CA^TATG
NgoMIV 158 GCCGGC G^CCGGC
NspI 1301 ACATGT [GA]CATG^[CT]
PleI 985 GACTC GAGTC(4/5)
PpuMI 56 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 1390 AACGTT AA^CGTT
RsrII 314 CGGACCG CG^G[AT]CCG
SacII 1149 CCGCGG CCGC^GG
SapI 300 GCTCTTC GCTCTTC(1/4)
SbfI 693 CCTGCAGG CCTGCA^GG
SmaI 900 CCCGGG CCC^GGG
Sse8387I 693 CCTGCAGG CCTGCA^GG
StuI 539 AGGCCT AGG^CCT
StyI 1269 CCAAGG C^C[AT][AT]GG
Tru9I 1220 TTAA T^TAA
Tsp45I 373 GTCAC ^GT[GC]AC
XcmI 191 CCAGCCTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 900 CCCGGG C^CCGGG
XmaI 900 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AspHI 30 GTGCTC G[AT]GC[AT]^C
109 GAGCAC
AspI 289 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
498 GACCAAGTC
AvaI 151 CCCGAG C^[CT]CG[GA]G
900 CCCGGG
BalI 203 TGGCCA TGG^CCA
1365 TGGCCA
BanI 136 GGCGCC G^G[CT][GA]CC
186 GGCGCC
BbeI 136 GGCGCC GGCGC^C
186 GGCGCC
BsaI 496 GAGACC GGTCTC(1/5)
781 GAGACC
BsiHKAI 30 GTGCTC G[AT]GC[AT]^C
109 GAGCAC
BsmAI 496 GAGAC GTCTC(1/5)
781 GAGAC
BsmFI 1229 GGGAC GGGAC(10/14)
852 GTCCC
BsoBI 151 CCCGAG C^[CT]CG[GA]G
900 CCCGGG
BssHII 256 GCGCGC G^CGCGC
320 GCGCGC
BstXI 206 CCAACATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1374 CCACGCCAATGG
BstYI 689 AGATCC [GA]^GATC[CT]
1136 AGATCC
DraIII 112 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG
871 CACCCAGTG
EaeI 203 TGGCCA [CT]^GGCC[GA]
1365 TGGCCA
KasI 136 GGCGCC G^GCGCC
186 GGCGCC
MflI 689 AGATCC [GA]^GATC[CT]
1136 AGATCC
MluNI 203 TGGCCA TGG^CCA
1365 TGGCCA
MscI 203 TGGCCA TGG^CCA
1365 TGGCCA
NarI 136 GGCGCC GG^CGCC
186 GGCGCC
PflFI 289 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
498 GACCAAGTC
PvuII 472 CAGCTG CAG^CTG
661 CAGCTG
SfoI 136 GGCGCC GGC^GCC
186 GGCGCC
Tth111I 289 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
498 GACCAAGTC
XhoII 689 AGATCC [GA]^GATC[CT]
1136 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI Aor51HI AvaII BanII
BbsI BbvI BcnI BmyI BpuAI
BsaHI BsaJI BsgI BsiYI BslI
Bsp1286I BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
Eco47III EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII HphI
ItaI MaeII MboI MboII MnlI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
TspRI TthHB8I