(Circular) (Six-base) MAPSORT of: pFN21AE1294.seq Check: 7373 from: 1 to: 6130 pFN21AE1294 6130 bp With 182 enzymes: SgfI * August 16, 2010 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3940 278 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3473 1937 Size: 1536 4594 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 3396 4257 4630 1563 Size: 1833 861 373 3063 Fragments arranged by size: 3063 1833 861 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 2441 3025 3054 3346 4193 5577 5845 Size: 785 24 584 29 292 847 1384 268 Cuts at: 5845 1632 Size: 1917 Fragments arranged by size: 1917 1384 847 785 584 292 268 29 24 AfeI AGC'GCT Cuts at: 2225 2472 2654 3002 2225 Size: 247 182 348 5353 Fragments arranged by size: 5353 348 247 182 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 3306 5029 6006 1203 Size: 2103 1723 977 1327 Fragments arranged by size: 2103 1723 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 4859 4859 Size: 6130 AleI CACnn'nnGTG Cuts at: 1166 2951 1166 Size: 1785 4345 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3191 1184 Size: 2007 4123 AlwNI CAG_nnn'CTG Cuts at: 2668 2698 2926 2983 3201 3406 3873 5445 2668 Size: 30 228 57 218 205 467 1572 3353 Fragments arranged by size: 3353 1572 467 228 218 205 57 30 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 3439 3665 3817 5926 1493 Size: 1946 226 152 2109 1697 Fragments arranged by size: 2109 1946 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2156 2905 3455 1870 Size: 73 213 749 550 4545 Fragments arranged by size: 4545 749 550 213 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2141 2191 3451 4906 618 Size: 325 217 627 354 50 1260 1455 1842 Fragments arranged by size: 1842 1455 1260 627 354 325 217 50 BanII G_rGCy'C Cuts at: 1391 2184 2692 3213 3449 1391 Size: 793 508 521 236 4072 Fragments arranged by size: 4072 793 521 508 236 BbeI G_GCGC'C Cuts at: 2145 2195 2145 Size: 50 6080 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2502 2811 3089 3250 928 Size: 971 603 309 278 161 3808 Fragments arranged by size: 3808 971 603 309 278 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2336 2370 4321 4355 1903 Size: 34 399 34 1951 34 3678 Fragments arranged by size: 3678 1951 399 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4022 5232 4022 Size: 1210 4920 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3129 4741 136 Size: 107 122 71 631 605 1457 1612 1525 Fragments arranged by size: 1612 1525 1457 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 2912 4191 5347 1790 Size: 1122 1279 1156 2573 Fragments arranged by size: 2573 1279 1156 1122 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2128 2873 3070 Size: 416 53 83 399 24 711 745 197 Cuts at: 3070 3082 3861 4819 442 Size: 12 779 958 1753 Fragments arranged by size: 1753 958 779 745 711 416 399 197 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2624 4790 1239 Size: 1385 2166 2579 Fragments arranged by size: 2579 2166 1385 Bpu10I CC'TnA_GC Cuts at: 2048 2048 Size: 6130 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2003 2186 2468 2753 4255 5120 5418 2003 Size: 183 282 285 1502 865 298 2715 Fragments arranged by size: 2715 1502 865 298 285 282 183 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2495 2780 4793 882 Size: 617 996 285 2013 2219 Fragments arranged by size: 2219 2013 996 617 285 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 2192 2247 2394 Size: 53 83 186 936 609 50 55 147 Cuts at: 2394 3051 3937 4319 4954 275 Size: 657 886 382 635 1451 Fragments arranged by size: 1451 936 886 657 635 609 382 186 147 83 55 53 50 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 1178 Size: 30 165 30 1089 30 4786 Fragments arranged by size: 4786 1089 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2612 1186 Size: 1426 4704 BseYI C'CCAG_C Cuts at: 2195 2741 3025 3149 5333 5829 2195 Size: 546 284 124 2184 496 2496 Fragments arranged by size: 2496 2184 546 496 284 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2032 2340 2345 2543 2929 3187 1163 Size: 573 296 308 5 198 386 258 4106 Fragments arranged by size: 4106 573 386 308 296 258 198 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2725 3580 4341 5369 1540 Size: 275 186 724 855 761 1028 2301 Fragments arranged by size: 2301 1028 855 761 724 275 186 BsiHKAI G_wGCw'C Cuts at: 2039 2118 4191 4276 5347 2039 Size: 79 2073 85 1071 2822 Fragments arranged by size: 2822 2073 1071 85 79 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 2118 2184 2692 2912 3213 3449 Size: 399 249 79 66 508 220 301 236 Cuts at: 3449 4191 4276 5347 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 301 249 236 220 85 79 66 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3485 844 Size: 2641 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2455 2554 2707 2764 4017 1394 Size: 429 632 99 153 57 1253 3507 Fragments arranged by size: 3507 1253 632 429 153 99 57 BsrDI GCAATG_nn' Cuts at: 65 1248 4625 4799 65 Size: 1183 3377 174 1396 Fragments arranged by size: 3377 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2163 2381 2562 2724 4774 4961 1700 Size: 110 353 218 181 162 2050 187 2869 Fragments arranged by size: 2869 2050 353 218 187 181 162 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6130 BssHII G'CGCG_C Cuts at: 1925 2261 2325 1925 Size: 336 64 5730 Fragments arranged by size: 5730 336 64 BssSI C'ACGA_G Cuts at: 2363 4190 5202 2363 Size: 1827 1012 3291 Fragments arranged by size: 3291 1827 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2122 2566 3351 794 Size: 1328 444 785 3573 Fragments arranged by size: 3573 1328 785 444 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 2377 1184 Size: 1193 4937 BstXI CCAn_nnnn'nTGG Cuts at: 2218 3386 2218 Size: 1168 4962 BstYI r'GATC_y Cuts at: 1972 2694 3141 3460 3827 4212 4229 5681 5767 Size: 722 447 319 367 385 17 1452 86 Cuts at: 5767 5779 6125 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 722 447 385 367 346 319 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3154 513 Size: 552 939 1150 3489 Fragments arranged by size: 3489 1150 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 2357 2981 3646 4440 4460 2357 Size: 624 665 794 20 4027 Fragments arranged by size: 4027 794 665 624 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6095 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DraIII CAC_nnn'GTG Cuts at: 2122 2881 2122 Size: 759 5371 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5137 809 Size: 507 3821 1802 Fragments arranged by size: 3821 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2208 3370 3577 Size: 54 1425 50 275 18 378 1162 207 Cuts at: 3577 4466 6095 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1162 889 378 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2312 4060 1353 Size: 959 1748 3423 Fragments arranged by size: 3423 1748 959 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2513 2624 3092 4717 5091 5237 Size: 1064 138 924 111 468 1625 374 146 Cuts at: 5237 387 Size: 1280 Fragments arranged by size: 1625 1280 1064 924 468 374 146 138 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2441 2624 3025 3054 3346 4193 Size: 393 785 24 183 401 29 292 847 Cuts at: 4193 4790 5577 5845 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 847 787 785 597 401 393 292 268 183 29 24 EcoNI CCTnn'n_nnAGG Cuts at: 2864 2864 Size: 6130 EcoO109I rG'GnC_Cy Cuts at: 2062 2437 2831 2062 Size: 375 394 5361 Fragments arranged by size: 5361 394 375 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FspI TGC'GCA Cuts at: 2672 4636 2672 Size: 1964 4166 HaeII r_GCGC'y Cuts at: 1678 2145 2195 2227 2474 2656 2926 3004 3201 Size: 467 50 32 247 182 270 78 197 Cuts at: 3201 5277 1678 Size: 2076 2531 Fragments arranged by size: 2531 2076 467 270 247 197 182 78 50 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2492 2524 2679 2711 3293 Size: 32 135 32 920 32 155 32 582 Cuts at: 3293 3325 4771 4803 4845 4877 1373 Size: 32 1446 32 42 32 2626 Fragments arranged by size: 2626 1446 920 582 155 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3474 3720 669 Size: 988 281 1536 246 3079 Fragments arranged by size: 3079 1536 988 281 246 HindIII A'AGCT_T Cuts at: 748 3239 748 Size: 2491 3639 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KasI G'GCGC_C Cuts at: 2141 2191 2141 Size: 50 6080 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2916 5244 5428 1305 Size: 1611 2328 184 2007 Fragments arranged by size: 2328 2007 1611 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 3372 10 Size: 54 1425 343 378 1162 2768 Fragments arranged by size: 2768 1425 1162 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2951 3173 3384 4088 4447 4606 518 Size: 648 1785 222 211 704 359 159 2042 Fragments arranged by size: 2042 1785 704 648 359 222 211 159 MspA1I CmG'CkG Cuts at: 1606 2103 2242 2479 2668 3156 3406 3546 4223 Size: 497 139 237 189 488 250 140 677 Cuts at: 4223 5371 5616 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 497 488 250 245 237 189 140 139 NaeI GCC'GGC Cuts at: 2165 2165 Size: 6130 NarI GG'CG_CC Cuts at: 2142 2192 2142 Size: 50 6080 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4639 Fragments arranged by size: 4639 939 552 NdeI CA'TA_TG Cuts at: 387 2800 387 Size: 2413 3717 NgoMIV G'CCGG_C Cuts at: 2163 2163 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 3310 3488 5033 1132 Size: 97 2081 178 1545 2229 Fragments arranged by size: 2229 2081 1545 178 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 3306 5029 3306 Size: 1723 4407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6130 PfoI T'CCnGG_A Cuts at: 2679 2865 3231 3261 2679 Size: 186 366 30 5548 Fragments arranged by size: 5548 366 186 30 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2062 Size: 6130 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 2412 2478 2703 2841 2988 3482 830 Size: 1582 66 225 138 147 494 3478 Fragments arranged by size: 3478 1582 494 225 147 138 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2479 2668 2479 Size: 189 5941 RsrII CG'GwC_CG Cuts at: 2320 2320 Size: 6130 SacII CC_GC'GG Cuts at: 3157 3157 Size: 6130 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SapI GCTCTTCn'nnn_ Cuts at: 2312 2312 Size: 6130 SbfI CC_TGCA'GG Cuts at: 2703 3482 2703 Size: 779 5351 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2227 2408 2474 2656 2699 2837 2984 Size: 219 1182 181 66 182 43 138 147 Cuts at: 2984 3478 4613 5294 5485 826 Size: 494 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 494 219 191 182 181 147 138 66 43 SfoI GGC'GCC Cuts at: 2143 2193 2143 Size: 50 6080 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 2907 3457 1872 Size: 1035 550 4545 Fragments arranged by size: 4545 1035 550 SmlI C'TyrA_G Cuts at: 820 1017 1943 2018 2201 2483 2768 4234 5135 Size: 197 926 75 183 282 285 1466 901 Cuts at: 5135 5397 820 Size: 262 1553 Fragments arranged by size: 1553 1466 926 901 285 282 262 197 183 75 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 2546 1517 Size: 1029 5101 StyI C'CwwG_G Cuts at: 513 1065 2004 3274 3569 513 Size: 552 939 1270 295 3074 Fragments arranged by size: 3074 1270 939 552 295 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2110 2645 2892 2988 3082 3242 4157 Size: 1255 530 535 247 96 94 160 915 Cuts at: 4157 4174 4327 4512 4957 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1255 915 535 530 445 247 185 160 153 96 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 2841 3192 Size: 274 80 33 51 1443 846 18 351 Cuts at: 3192 4376 6088 96 Size: 1184 1712 138 Fragments arranged by size: 1712 1443 1184 846 351 274 138 80 51 33 18 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6090 6122 6090 Size: 32 6098 Tth111I GACn'n_nGTC Cuts at: 1316 2297 2506 1316 Size: 981 209 4940 Fragments arranged by size: 4940 981 209 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2203 Size: 6130 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 2905 3455 1870 Size: 1035 550 4545 Fragments arranged by size: 4545 1035 550 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3938 276 Size: 53 83 186 936 2404 2468 Fragments arranged by size: 2468 2404 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII RsrII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI SacI SanDI SexAI SfiI SrfI SwaI