(Linear) (Six-base) MAPSORT of: KIEE1294.seq Check: 7487 from: 1 to: 1425 KIEE1294.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AclI AA'CG_TT Cuts at: 0 1391 1425 Size: 1391 34 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 436 1020 1049 1341 1425 Size: 412 24 584 29 292 84 Fragments arranged by size: 584 412 292 84 29 24 AfeI AGC'GCT Cuts at: 0 220 467 649 997 1425 Size: 220 247 182 348 428 Fragments arranged by size: 428 348 247 220 182 AflIII A'CryG_T Cuts at: 0 1301 1425 Size: 1301 124 AleI CACnn'nnGTG Cuts at: 0 946 1425 Size: 946 479 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1186 1425 Size: 1186 239 AlwNI CAG_nnn'CTG Cuts at: 0 663 693 921 978 1196 1401 1425 Size: 663 30 228 57 218 205 24 Fragments arranged by size: 663 228 218 205 57 30 24 AvaI C'yCGr_G Cuts at: 0 151 900 1425 Size: 151 749 525 Fragments arranged by size: 749 525 151 BanI G'GyrC_C Cuts at: 0 136 186 1425 Size: 136 50 1239 Fragments arranged by size: 1239 136 50 BanII G_rGCy'C Cuts at: 0 179 687 1208 1425 Size: 179 508 521 217 Fragments arranged by size: 521 508 217 179 BbeI G_GCGC'C Cuts at: 0 140 190 1425 Size: 140 50 1235 Fragments arranged by size: 1235 140 50 BbsI GAAGACnn'nnnn_ Cuts at: 0 497 806 1084 1245 1425 Size: 497 309 278 161 180 Fragments arranged by size: 497 309 278 180 161 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 331 365 1425 Size: 331 34 1060 Fragments arranged by size: 1060 331 34 BglI GCCn_nnn'nGGC Cuts at: 0 1124 1425 Size: 1124 301 Bme1580I G_kGCm'C Cuts at: 0 907 1425 Size: 907 518 BmrI ACTGGGnnnn_n' Cuts at: 0 123 868 1065 1077 1425 Size: 123 745 197 12 348 Fragments arranged by size: 745 348 197 123 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 619 1425 Size: 619 806 Bpu10I CC'TnA_GC Cuts at: 0 43 1425 Size: 43 1382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 463 748 1425 Size: 181 282 285 677 Fragments arranged by size: 677 285 282 181 BsaI GGTCTCn'nnnn_ Cuts at: 0 490 775 1425 Size: 490 285 650 Fragments arranged by size: 650 490 285 BsaHI Gr'CG_yC Cuts at: 0 137 187 242 389 1046 1425 Size: 137 50 55 147 657 379 Fragments arranged by size: 657 379 147 137 55 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 487 517 1425 Size: 487 30 908 Fragments arranged by size: 908 487 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 607 1425 Size: 607 818 BseYI C'CCAG_C Cuts at: 0 190 736 1020 1144 1425 Size: 190 546 284 124 281 Fragments arranged by size: 546 284 281 190 124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 27 335 340 538 924 1182 1425 Size: 27 308 5 198 386 258 243 Fragments arranged by size: 386 308 258 243 198 27 5 BsiEI CG_ry'CG Cuts at: 0 720 1425 Size: 720 705 BsiHKAI G_wGCw'C Cuts at: 0 34 113 1425 Size: 34 79 1312 Fragments arranged by size: 1312 79 34 Bsp1286I G_dGCh'C Cuts at: 0 34 113 179 687 907 1208 1425 Size: 34 79 66 508 220 301 217 Fragments arranged by size: 508 301 220 217 79 66 34 BsrBI CCG'CTC Cuts at: 0 450 549 702 759 1425 Size: 450 99 153 57 666 Fragments arranged by size: 666 450 153 99 57 BsrFI r'CCGG_y Cuts at: 0 158 376 557 719 1425 Size: 158 218 181 162 706 Fragments arranged by size: 706 218 181 162 158 BssHII G'CGCG_C Cuts at: 0 256 320 1425 Size: 256 64 1105 Fragments arranged by size: 1105 256 64 BssSI C'ACGA_G Cuts at: 0 358 1425 Size: 358 1067 BstAPI GCAn_nnn'nTGC Cuts at: 0 117 561 1346 1425 Size: 117 444 785 79 Fragments arranged by size: 785 444 117 79 BstEII G'GTnAC_C Cuts at: 0 372 1425 Size: 372 1053 BstXI CCAn_nnnn'nTGG Cuts at: 0 213 1381 1425 Size: 213 1168 44 Fragments arranged by size: 1168 213 44 BstYI r'GATC_y Cuts at: 0 689 1136 1425 Size: 689 447 289 Fragments arranged by size: 689 447 289 BtgI C'CryG_G Cuts at: 0 1149 1425 Size: 1149 276 BtsI GCAGTG_nn' Cuts at: 0 352 976 1425 Size: 352 624 449 Fragments arranged by size: 624 449 352 DraIII CAC_nnn'GTG Cuts at: 0 117 876 1425 Size: 117 759 549 Fragments arranged by size: 759 549 117 EaeI y'GGCC_r Cuts at: 0 203 1365 1425 Size: 203 1162 60 Fragments arranged by size: 1162 203 60 EarI CTCTTCn'nnn_ Cuts at: 0 307 1425 Size: 307 1118 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 508 619 1087 1425 Size: 508 111 468 338 Fragments arranged by size: 508 468 338 111 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 412 436 619 1020 1049 1341 1425 Size: 412 24 183 401 29 292 84 Fragments arranged by size: 412 401 292 183 84 29 24 EcoNI CCTnn'n_nnAGG Cuts at: 0 859 1425 Size: 859 566 EcoO109I rG'GnC_Cy Cuts at: 0 57 432 826 1425 Size: 57 375 394 599 Fragments arranged by size: 599 394 375 57 FspI TGC'GCA Cuts at: 0 667 1425 Size: 667 758 HaeII r_GCGC'y Cuts at: 0 140 190 222 469 651 921 999 1196 Size: 140 50 32 247 182 270 78 197 Cuts at: 1196 1425 Size: 229 Fragments arranged by size: 270 247 229 197 182 140 78 50 32 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 487 519 674 706 1288 1320 1425 Size: 487 32 155 32 582 32 105 Fragments arranged by size: 582 487 155 105 32 32 32 HindIII A'AGCT_T Cuts at: 0 1234 1425 Size: 1234 191 KasI G'GCGC_C Cuts at: 0 136 186 1425 Size: 136 50 1239 Fragments arranged by size: 1239 136 50 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 911 1425 Size: 911 514 MscI TGG'CCA Cuts at: 0 205 1367 1425 Size: 205 1162 58 Fragments arranged by size: 1162 205 58 MslI CAynn'nnrTG Cuts at: 0 946 1168 1379 1425 Size: 946 222 211 46 Fragments arranged by size: 946 222 211 46 MspA1I CmG'CkG Cuts at: 0 98 237 474 663 1151 1401 1425 Size: 98 139 237 189 488 250 24 Fragments arranged by size: 488 250 237 189 139 98 24 NaeI GCC'GGC Cuts at: 0 160 1425 Size: 160 1265 NarI GG'CG_CC Cuts at: 0 137 187 1425 Size: 137 50 1238 Fragments arranged by size: 1238 137 50 NdeI CA'TA_TG Cuts at: 0 795 1425 Size: 795 630 NgoMIV G'CCGG_C Cuts at: 0 158 1425 Size: 158 1267 NspI r_CATG'y Cuts at: 0 1305 1425 Size: 1305 120 PciI A'CATG_T Cuts at: 0 1301 1425 Size: 1301 124 PfoI T'CCnGG_A Cuts at: 0 674 860 1226 1256 1425 Size: 674 186 366 30 169 Fragments arranged by size: 674 366 186 169 30 PpuMI rG'GwC_Cy Cuts at: 0 57 1425 Size: 57 1368 PstI C_TGCA'G Cuts at: 0 407 473 698 836 983 1425 Size: 407 66 225 138 147 442 Fragments arranged by size: 442 407 225 147 138 66 PvuII CAG'CTG Cuts at: 0 474 663 1425 Size: 474 189 762 Fragments arranged by size: 762 474 189 RsrII CG'GwC_CG Cuts at: 0 315 1425 Size: 315 1110 SacII CC_GC'GG Cuts at: 0 1152 1425 Size: 1152 273 SapI GCTCTTCn'nnn_ Cuts at: 0 307 1425 Size: 307 1118 SbfI CC_TGCA'GG Cuts at: 0 698 1425 Size: 698 727 SfcI C'TryA_G Cuts at: 0 222 403 469 651 694 832 979 1425 Size: 222 181 66 182 43 138 147 446 Fragments arranged by size: 446 222 182 181 147 138 66 43 SfoI GGC'GCC Cuts at: 0 138 188 1425 Size: 138 50 1237 Fragments arranged by size: 1237 138 50 SmaI CCC'GGG Cuts at: 0 902 1425 Size: 902 523 SmlI C'TyrA_G Cuts at: 0 13 196 478 763 1425 Size: 13 183 282 285 662 Fragments arranged by size: 662 285 282 183 13 StuI AGG'CCT Cuts at: 0 541 1425 Size: 541 884 StyI C'CwwG_G Cuts at: 0 1269 1425 Size: 1269 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 105 640 887 983 1077 1237 1425 Size: 105 535 247 96 94 160 188 Fragments arranged by size: 535 247 188 160 105 96 94 TatI w'GTAC_w Cuts at: 0 818 836 1187 1425 Size: 818 18 351 238 Fragments arranged by size: 818 351 238 18 Tth111I GACn'n_nGTC Cuts at: 0 292 501 1425 Size: 292 209 924 Fragments arranged by size: 924 292 209 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 198 1425 Size: 198 1227 XmaI C'CCGG_G Cuts at: 0 900 1425 Size: 900 525 Enzymes that do cut: AclI AcuI AfeI AflIII AleI AlfI AlwNI AvaI BanI BanII BbeI BbsI BcgI BglI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I HindIII KasI MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI PfoI PpuMI PstI PvuII RsrII SacII SapI SbfI SfcI SfoI SmaI SmlI StuI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AflII AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrGI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII HpaI KpnI MfeI MluI NcoI NheI NotI NruI NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI SacI SalI SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI TstI XbaI XhoI XmnI ZraI