################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AvaI AviII AvrII BbrPI BbvCI BcgI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HpaI KpnI KspI MfeI MluI MroI MslI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AcyI 34 GGCGCC G[GA]^CG[CT]C ApaI 367 GGGCCC GGGCC^C AspI 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 2 TGGCCA TGG^CCA BamHI 97 GGATCC G^GATCC BanI 34 GGCGCC G^G[CT][GA]CC BbeI 34 GGCGCC GGCGC^C BbsI 166 GAAGAC GAAGAC(2/6) BciVI 350 GGATAC GTATCC(6/5) BglI 37 GCCGCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 166 GAAGAC GAAGAC(2/6) BsaBI 877 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 34 GGCGCC G[GA]^CG[CT]C BsaI 1125 GAGACC GGTCTC(1/5) BsgI 1219 GTGCAG GTGCAG(16/14) BspMI 1411 GCAGGT ACCTGC(4/8) BsrFI 962 GCCGGC [GA]^CCGG[CT] BstEII 193 GGTGACC G^GT[ACGT]ACC BstPI 193 GGTGACC G^GT[ACGT]ACC Cfr10I 962 GCCGGC [GA]^CCGG[CT] EaeI 2 TGGCCA [CT]^GGCC[GA] EcoO65I 193 GGTGACC G^GT[ACGT]ACC FauI 26 GCGGG CCCGC(4/6) HaeII 34 GGCGCC [GA]GCGC^[CT] Hin1I 34 GGCGCC G[GA]^CG[CT]C HindIII 733 AAGCTT A^AGCTT HphI 193 GGTGA GGTGA(8/7) KasI 34 GGCGCC G^GCGCC MamI 877 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA NaeI 962 GCCGGC GCC^GGC NarI 34 GGCGCC GG^CGCC NgoMIV 962 GCCGGC G^CCGGC NspI 285 GCATGT [GA]CATG^[CT] PflFI 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC PleI 968 GACTC GAGTC(4/5) SacI 1342 GAGCTC GAGCT^C SapI 1339 GAAGAGC GCTCTTC(1/4) SbfI 621 CCTGCAGG CCTGCA^GG SfoI 34 GGCGCC GGC^GCC SmlI 1433 CTCAAG C^T[CT][GA]AG Sse8387I 621 CCTGCAGG CCTGCA^GG TaqI 164 TCGA T^CGA TfiI 303 GAATC G^A[AT]TC Tth111I 1425 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 164 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 22 CGCG CG^CG 48 CGCG AfaI 631 GTAC GT^AC 1227 GTAC AlwNI 1009 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG 1036 CAGTTCCTG AocI 451 CCTGAGG CC^T[ACGT]AGG 702 CCTAAGG Asp700I 166 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 732 GAAGCTTTTC AspHI 400 GAGCAC G[AT]GC[AT]^C 1342 GAGCTC BsiHKAI 400 GAGCAC G[AT]GC[AT]^C 1342 GAGCTC BsmFI 29 GGGAC GGGAC(10/14) 1069 GGGAC BsrI 861 ACTGG ACTGG(1/-1) 978 CCAGT BstUI 22 CGCG CG^CG 48 CGCG BstXI 933 CCAGAGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1373 CCAAGAGCCTGG Bsu36I 451 CCTGAGG CC^T[ACGT]AGG 702 CCTAAGG CfoI 21 GCGC GCG^C 35 GCGC DsaI 335 CCACGG C^C[GA][CT]GG 371 CCGTGG Eco81I 451 CCTGAGG CC^T[ACGT]AGG 702 CCTAAGG HhaI 21 GCGC GCG^C 35 GCGC HinP1I 21 GCGC G^CGC 35 GCGC HinfI 303 GAATC G^A[ACGT]TC 968 GACTC MaeII 404 ACGT A^CGT 797 ACGT MseI 157 TTAA T^TAA 1270 TTAA MvnI 22 CGCG CG^CG 48 CGCG PflMI 549 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1008 CCAGGACCTGG PstI 622 CTGCAG CTGCA^G 1081 CTGCAG RsaI 631 GTAC GT^AC 1227 GTAC SfaNI 877 GATGC GCATC(5/9) 1393 GATGC SfcI 622 CTGCAG C^T[GA][CT]AG 1081 CTGCAG Tru9I 157 TTAA T^TAA 1270 TTAA Tsp509I 73 AATT ^AATT 518 AATT TspRI 317 CAGTG CA[GC]TG[ACGT][ACGT]^ 541 CAGTG Van91I 549 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1008 CCAGGACCTGG XmnI 166 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 732 GAAGCTTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 58 enzymes) # ######################################### AciI AluI AlwI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HpaII ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI PvuII Sau3AI Sau96I ScrFI TseI Tsp45I XhoII