(Circular) (Six-base) MAPSORT of: pFN21AE1247.seq Check: 2645 from: 1 to: 6151 pFN21AE1247 6151 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3407 3407 Size: 6151 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3961 278 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3494 1937 Size: 1557 4594 Acc65I G'GTAC_C Cuts at: 3472 3472 Size: 6151 AclI AA'CG_TT Cuts at: 1563 4278 4651 1563 Size: 2715 373 3063 Fragments arranged by size: 3063 2715 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2133 3363 4214 5598 5866 1632 Size: 501 1230 851 1384 268 1917 Fragments arranged by size: 1917 1384 1230 851 501 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5954 AflIII A'CryG_T Cuts at: 1203 5050 6027 1203 Size: 3847 977 1327 Fragments arranged by size: 3847 1327 977 AgeI A'CCGG_T Cuts at: 4982 4982 Size: 6151 AhdI GACnn_n'nnGTC Cuts at: 4880 4880 Size: 6151 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6151 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6151 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2087 2119 2087 Size: 32 6119 AlwNI CAG_nnn'CTG Cuts at: 3019 3046 3894 5466 3019 Size: 27 848 1572 3704 Fragments arranged by size: 3704 1572 848 27 ApaI G_GGCC'C Cuts at: 2376 2376 Size: 6151 ApaLI G'TGCA_C Cuts at: 4208 5364 4208 Size: 1156 4995 ApoI r'AATT_y Cuts at: 1493 3460 3686 3838 5947 1493 Size: 1967 226 152 2109 1697 Fragments arranged by size: 2109 1967 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4705 160 Size: 872 3673 1606 Fragments arranged by size: 3673 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 AvaI C'yCGr_G Cuts at: 1870 1943 3476 1870 Size: 73 1533 4545 Fragments arranged by size: 4545 1533 73 BamHI G'GATC_C Cuts at: 2102 3481 3848 2102 Size: 1379 367 4405 Fragments arranged by size: 4405 1379 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2039 3472 4927 618 Size: 325 217 627 252 1433 1455 1842 Fragments arranged by size: 1842 1455 1433 627 325 252 217 BanII G_rGCy'C Cuts at: 1391 2022 2376 2486 3351 3470 1391 Size: 631 354 110 865 119 4072 Fragments arranged by size: 4072 865 631 354 119 110 BbeI G_GCGC'C Cuts at: 2043 2043 Size: 6151 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 928 Size: 971 279 4901 Fragments arranged by size: 4901 971 279 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4342 4376 1903 Size: 34 2405 34 3678 Fragments arranged by size: 3678 2405 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2349 4043 5253 2349 Size: 1694 1210 3247 Fragments arranged by size: 3247 1694 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6151 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 4762 136 Size: 107 122 71 631 605 376 2714 1525 Fragments arranged by size: 2714 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 6146 6146 Size: 6151 BlpI GC'TnA_GC Cuts at: 3568 3568 Size: 6151 Bme1580I G_kGCm'C Cuts at: 1790 2376 2952 4212 5368 1790 Size: 586 576 1260 1156 2573 Fragments arranged by size: 2573 1260 1156 586 576 BmgBI CAC'GTC Cuts at: 1318 2803 1318 Size: 1485 4666 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2977 3882 4840 Size: 416 53 83 399 24 1560 905 958 Cuts at: 4840 442 Size: 1753 Fragments arranged by size: 1753 1560 958 905 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6151 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2714 2804 2942 3041 3200 3266 3278 Size: 968 507 90 138 99 159 66 12 Cuts at: 3278 4811 1239 Size: 1533 2579 Fragments arranged by size: 2579 1533 968 507 159 138 99 90 66 12 Bpu10I CC'TnA_GC Cuts at: 2504 3326 2504 Size: 822 5329 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3423 4276 5141 5439 3423 Size: 853 865 298 4135 Fragments arranged by size: 4135 865 853 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3124 4814 882 Size: 617 1625 1690 2219 Fragments arranged by size: 2219 1690 1625 617 BsaAI yAC'GTr Cuts at: 493 1204 3942 493 Size: 711 2738 2702 Fragments arranged by size: 2738 2702 711 BsaBI GATnn'nnATC Cuts at: 2886 6145 2886 Size: 3259 2892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2040 3958 4340 4975 Size: 53 83 186 936 507 1918 382 635 Cuts at: 4975 275 Size: 1451 Fragments arranged by size: 1918 1451 936 635 507 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4584 4982 5256 5403 5814 4584 Size: 398 274 147 411 4921 Fragments arranged by size: 4921 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2237 2267 2309 2339 2415 Size: 30 165 30 834 30 42 30 76 Cuts at: 2415 2445 3014 3044 3419 3449 1178 Size: 30 569 30 375 30 3880 Fragments arranged by size: 3880 834 569 375 165 76 42 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2073 2258 2276 2312 2330 2474 2510 2591 Size: 887 185 18 36 18 144 36 81 Cuts at: 2591 2729 3038 3041 1186 Size: 138 309 3 4296 Fragments arranged by size: 4296 887 309 185 144 138 81 36 36 18 18 3 BseYI C'CCAG_C Cuts at: 2486 2565 2850 2871 3071 5354 5850 2486 Size: 79 285 21 200 2283 496 2787 Fragments arranged by size: 2787 2283 496 285 200 79 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3245 1163 Size: 573 1509 4069 Fragments arranged by size: 4069 1509 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3601 4362 5390 1540 Size: 275 186 1600 761 1028 2301 Fragments arranged by size: 2301 1600 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2409 3351 4212 4297 5368 2409 Size: 942 861 85 1071 3192 Fragments arranged by size: 3192 1071 942 861 85 BsmI GAATG_Cn' Cuts at: 3662 3755 3662 Size: 93 6058 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2376 2409 2486 2952 3351 3470 Size: 399 232 354 33 77 466 399 119 Cuts at: 3470 4212 4297 5368 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 466 399 399 354 232 119 85 77 33 BspEI T'CCGG_A Cuts at: 5814 5814 Size: 6151 BspHI T'CATG_A Cuts at: 4040 5770 4040 Size: 1730 4421 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3407 3506 844 Size: 2563 99 3489 Fragments arranged by size: 3489 2563 99 BsrBI CCG'CTC Cuts at: 1394 1823 4038 1394 Size: 429 2215 3507 Fragments arranged by size: 3507 2215 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4646 4820 65 Size: 1183 3398 174 1396 Fragments arranged by size: 3398 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2967 4795 4982 1700 Size: 110 1157 1828 187 2869 Fragments arranged by size: 2869 1828 1157 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6151 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6151 BssSI C'ACGA_G Cuts at: 4211 5223 4211 Size: 1012 5139 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6151 BstBI TT'CG_AA Cuts at: 4955 4955 Size: 6151 BstEII G'GTnAC_C Cuts at: 1184 2198 1184 Size: 1014 5137 BstXI CCAn_nnnn'nTGG Cuts at: 2945 3385 2945 Size: 440 5711 BstYI r'GATC_y Cuts at: 1972 2102 2718 3105 3481 3848 4233 4250 5702 Size: 130 616 387 376 367 385 17 1452 Cuts at: 5702 5788 5800 6146 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 616 387 385 376 367 346 130 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2457 2708 2457 Size: 251 5900 BtgI C'CryG_G Cuts at: 513 1065 2004 2340 2376 513 Size: 552 939 336 36 4288 Fragments arranged by size: 4288 939 552 336 36 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5412 Fragments arranged by size: 5412 683 56 BtsI GCAGTG_nn' Cuts at: 2552 3667 4461 4481 2552 Size: 1115 794 20 4222 Fragments arranged by size: 4222 1115 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6151 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6116 DraI TTT'AAA Cuts at: 3455 3455 Size: 6151 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5158 809 Size: 507 3842 1802 Fragments arranged by size: 3842 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3598 4487 Size: 54 1425 50 275 18 177 1591 889 Cuts at: 4487 6116 8 Size: 1629 43 Fragments arranged by size: 1629 1591 1425 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3598 1537 Size: 275 1786 4090 Fragments arranged by size: 4090 1786 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2091 2568 3213 3339 4081 1353 Size: 738 477 645 126 742 3423 Fragments arranged by size: 3423 742 738 645 477 126 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2456 3149 4738 5112 5258 387 Size: 1064 138 867 693 1589 374 146 1280 Fragments arranged by size: 1589 1280 1064 867 693 374 146 138 EcoICRI GAG'CTC Cuts at: 3349 3349 Size: 6151 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2133 2207 2714 2804 2942 3041 3200 Size: 393 501 74 507 90 138 99 159 Cuts at: 3200 3266 3278 3363 4214 4811 5598 5866 1239 Size: 66 12 85 851 597 787 268 1524 Fragments arranged by size: 1524 851 787 597 507 501 393 268 159 138 99 90 85 74 66 12 EcoNI CCTnn'n_nnAGG Cuts at: 2111 2630 2711 3023 3389 2111 Size: 519 81 312 366 4873 Fragments arranged by size: 4873 519 366 312 81 EcoO109I rG'GnC_Cy Cuts at: 2157 2248 2372 2515 3016 2157 Size: 91 124 143 501 5292 Fragments arranged by size: 5292 501 143 124 91 EcoRI G'AATT_C Cuts at: 3460 3838 3460 Size: 378 5773 FspI TGC'GCA Cuts at: 4657 4657 Size: 6151 HaeII r_GCGC'y Cuts at: 1678 2043 5298 1678 Size: 365 3255 2531 Fragments arranged by size: 3255 2531 365 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2415 2447 3067 3099 3419 Size: 32 135 32 843 32 620 32 320 Cuts at: 3419 3421 3451 3453 4792 4824 4866 4898 1373 Size: 2 30 2 1339 32 42 32 2626 Fragments arranged by size: 2626 1339 843 620 320 135 42 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 3495 3741 669 Size: 988 281 1557 246 3079 Fragments arranged by size: 3079 1557 988 281 246 HindIII A'AGCT_T Cuts at: 748 2738 748 Size: 1990 4161 HpaI GTT'AAC Cuts at: 3741 3741 Size: 6151 KasI G'GCGC_C Cuts at: 2039 2039 Size: 6151 KpnI G_GTAC'C Cuts at: 3476 3476 Size: 6151 MfeI C'AATT_G Cuts at: 3750 3750 Size: 6151 MluI A'CGCG_T Cuts at: 6027 6027 Size: 6151 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5265 5449 1305 Size: 3960 184 2007 Fragments arranged by size: 3960 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 4152 Fragments arranged by size: 4152 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 4109 4468 4627 518 Size: 648 2943 359 159 2042 Fragments arranged by size: 2943 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2569 2782 2908 3244 3567 4244 5392 5637 Size: 963 213 126 336 323 677 1148 245 Cuts at: 5637 1606 Size: 2120 Fragments arranged by size: 2120 1148 963 677 336 323 245 213 126 NaeI GCC'GGC Cuts at: 2969 2969 Size: 6151 NarI GG'CG_CC Cuts at: 2040 2040 Size: 6151 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4660 Fragments arranged by size: 4660 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6151 NgoMIV G'CCGG_C Cuts at: 2967 2967 Size: 6151 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6151 NotI GC'GGCC_GC Cuts at: 3598 3598 Size: 6151 NspI r_CATG'y Cuts at: 1132 1229 2294 3509 5054 1132 Size: 97 1065 1215 1545 2229 Fragments arranged by size: 2229 1545 1215 1065 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6151 PciI A'CATG_T Cuts at: 5050 5050 Size: 6151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2560 3019 1108 Size: 1452 459 4240 Fragments arranged by size: 4240 1452 459 PfoI T'CCnGG_A Cuts at: 2613 2823 2877 2958 3108 2613 Size: 210 54 81 150 5656 Fragments arranged by size: 5656 210 150 81 54 PmeI GTTT'AAAC Cuts at: 3455 3455 Size: 6151 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3026 3058 4220 4252 5733 5765 3026 Size: 32 1162 32 1481 32 3412 Fragments arranged by size: 3412 1481 1162 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2157 2515 3016 2157 Size: 358 501 5292 Fragments arranged by size: 5292 501 358 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6151 PsiI TTA'TAA Cuts at: 3360 3721 3360 Size: 361 5790 PspOMI G'GGCC_C Cuts at: 2372 2372 Size: 6151 PstI C_TGCA'G Cuts at: 830 2631 3090 3503 830 Size: 1801 459 413 3478 Fragments arranged by size: 3478 1801 459 413 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6151 PvuII CAG'CTG Cuts at: 2569 2782 2908 3244 2569 Size: 213 126 336 5476 Fragments arranged by size: 5476 336 213 126 SacI G_AGCT'C Cuts at: 3351 3351 Size: 6151 SalI G'TCGA_C Cuts at: 1936 3493 1936 Size: 1557 4594 SapI GCTCTTCn'nnn_ Cuts at: 3339 3339 Size: 6151 SbfI CC_TGCA'GG Cuts at: 2631 3503 2631 Size: 872 5279 ScaI AGT'ACT Cuts at: 4399 6111 4399 Size: 1712 4439 SfcI C'TryA_G Cuts at: 826 1045 2627 3086 3499 4634 5315 5506 826 Size: 219 1582 459 413 1135 681 191 1471 Fragments arranged by size: 1582 1471 1135 681 459 413 219 191 SfoI GGC'GCC Cuts at: 2041 2041 Size: 6151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6151 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6151 SmaI CCC'GGG Cuts at: 1872 3478 1872 Size: 1606 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3438 4255 5156 5418 820 Size: 197 926 1495 817 901 262 1553 Fragments arranged by size: 1553 1495 926 901 817 262 197 SnaBI TAC'GTA Cuts at: 493 3942 493 Size: 3449 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6151 SphI G_CATG'C Cuts at: 1132 3509 1132 Size: 2377 3774 SspI AAT'ATT Cuts at: 5 52 4075 5 Size: 47 4023 2081 Fragments arranged by size: 4023 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6151 StyI C'CwwG_G Cuts at: 513 1065 2004 3590 513 Size: 552 939 1586 3074 Fragments arranged by size: 3074 1586 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4178 4195 4348 4533 4978 325 Size: 1255 2598 17 153 185 445 1498 Fragments arranged by size: 2598 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3231 4397 6109 Size: 274 80 33 51 1443 1254 1166 1712 Cuts at: 6109 96 Size: 138 Fragments arranged by size: 1712 1443 1254 1166 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 6111 6143 2309 Size: 32 3770 32 2317 Fragments arranged by size: 3770 2317 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3433 1316 Size: 2117 4034 XbaI T'CTAG_A Cuts at: 3487 3487 Size: 6151 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6151 XmaI C'CCGG_G Cuts at: 1870 3476 1870 Size: 1606 4545 XmnI GAAnn'nnTTC Cuts at: 2175 2741 4280 2175 Size: 566 1539 4046 Fragments arranged by size: 4046 1539 566 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3959 276 Size: 53 83 186 936 2425 2468 Fragments arranged by size: 2468 2425 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI