(Linear) (Six-base) MAPSORT of: KIEE1247.seq Check: 241 from: 1 to: 1446 KIEE1247.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1402 1446 Size: 1402 44 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 1358 1446 Size: 128 1230 88 Fragments arranged by size: 1230 128 88 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 82 114 1446 Size: 82 32 1332 Fragments arranged by size: 1332 82 32 AlwNI CAG_nnn'CTG Cuts at: 0 1014 1041 1446 Size: 1014 27 405 Fragments arranged by size: 1014 405 27 ApaI G_GGCC'C Cuts at: 0 371 1446 Size: 371 1075 BamHI G'GATC_C Cuts at: 0 97 1446 Size: 97 1349 BanI G'GyrC_C Cuts at: 0 34 1446 Size: 34 1412 BanII G_rGCy'C Cuts at: 0 17 371 481 1346 1446 Size: 17 354 110 865 100 Fragments arranged by size: 865 354 110 100 17 BbeI G_GCGC'C Cuts at: 0 38 1446 Size: 38 1408 BbsI GAAGACnn'nnnn_ Cuts at: 0 173 1446 Size: 173 1273 BciVI GTATCCnnnnn_n' Cuts at: 0 344 1446 Size: 344 1102 BglI GCCn_nnn'nGGC Cuts at: 0 43 1446 Size: 43 1403 Bme1580I G_kGCm'C Cuts at: 0 371 947 1446 Size: 371 576 499 Fragments arranged by size: 576 499 371 BmgBI CAC'GTC Cuts at: 0 798 1446 Size: 798 648 BmrI ACTGGGnnnn_n' Cuts at: 0 972 1446 Size: 972 474 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 709 799 937 1036 1195 1261 1273 Size: 202 507 90 138 99 159 66 12 Cuts at: 1273 1446 Size: 173 Fragments arranged by size: 507 202 173 159 138 99 90 66 12 Bpu10I CC'TnA_GC Cuts at: 0 499 1321 1446 Size: 499 822 125 Fragments arranged by size: 822 499 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1418 1446 Size: 1418 28 BsaI GGTCTCn'nnnn_ Cuts at: 0 1119 1446 Size: 1119 327 BsaBI GATnn'nnATC Cuts at: 0 881 1446 Size: 881 565 BsaHI Gr'CG_yC Cuts at: 0 35 1446 Size: 35 1411 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 232 262 304 334 410 440 1009 1039 Size: 232 30 42 30 76 30 569 30 Cuts at: 1039 1414 1444 1446 Size: 375 30 2 Fragments arranged by size: 569 375 232 76 42 30 30 30 30 30 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 68 253 271 307 325 469 505 586 Size: 68 185 18 36 18 144 36 81 Cuts at: 586 724 1033 1036 1446 Size: 138 309 3 410 Fragments arranged by size: 410 309 185 144 138 81 68 36 36 18 18 3 BseYI C'CCAG_C Cuts at: 0 481 560 845 866 1066 1446 Size: 481 79 285 21 200 380 Fragments arranged by size: 481 380 285 200 79 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1240 1446 Size: 1240 206 BsiHKAI G_wGCw'C Cuts at: 0 404 1346 1446 Size: 404 942 100 Fragments arranged by size: 942 404 100 Bsp1286I G_dGCh'C Cuts at: 0 17 371 404 481 947 1346 1446 Size: 17 354 33 77 466 399 100 Fragments arranged by size: 466 399 354 100 77 33 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1402 1446 Size: 1402 44 BsrFI r'CCGG_y Cuts at: 0 962 1446 Size: 962 484 BstEII G'GTnAC_C Cuts at: 0 193 1446 Size: 193 1253 BstXI CCAn_nnnn'nTGG Cuts at: 0 940 1380 1446 Size: 940 440 66 Fragments arranged by size: 940 440 66 BstYI r'GATC_y Cuts at: 0 97 713 1100 1446 Size: 97 616 387 346 Fragments arranged by size: 616 387 346 97 Bsu36I CC'TnA_GG Cuts at: 0 452 703 1446 Size: 452 251 743 Fragments arranged by size: 743 452 251 BtgI C'CryG_G Cuts at: 0 335 371 1446 Size: 335 36 1075 Fragments arranged by size: 1075 335 36 BtsI GCAGTG_nn' Cuts at: 0 547 1446 Size: 547 899 EaeI y'GGCC_r Cuts at: 0 2 1446 Size: 2 1444 EarI CTCTTCn'nnn_ Cuts at: 0 86 563 1208 1334 1446 Size: 86 477 645 126 112 Fragments arranged by size: 645 477 126 112 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 1144 1446 Size: 451 693 302 Fragments arranged by size: 693 451 302 EcoICRI GAG'CTC Cuts at: 0 1344 1446 Size: 1344 102 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 128 202 709 799 937 1036 1195 1261 Size: 128 74 507 90 138 99 159 66 Cuts at: 1261 1273 1358 1446 Size: 12 85 88 Fragments arranged by size: 507 159 138 128 99 90 88 85 74 66 12 EcoNI CCTnn'n_nnAGG Cuts at: 0 106 625 706 1018 1384 1446 Size: 106 519 81 312 366 62 Fragments arranged by size: 519 366 312 106 81 62 EcoO109I rG'GnC_Cy Cuts at: 0 152 243 367 510 1011 1446 Size: 152 91 124 143 501 435 Fragments arranged by size: 501 435 152 143 124 91 HaeII r_GCGC'y Cuts at: 0 38 1446 Size: 38 1408 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 410 442 1062 1094 1414 1416 1446 Size: 410 32 620 32 320 2 30 Fragments arranged by size: 620 410 320 32 32 30 2 HindIII A'AGCT_T Cuts at: 0 733 1446 Size: 733 713 KasI G'GCGC_C Cuts at: 0 34 1446 Size: 34 1412 MscI TGG'CCA Cuts at: 0 4 1446 Size: 4 1442 MspA1I CmG'CkG Cuts at: 0 564 777 903 1239 1446 Size: 564 213 126 336 207 Fragments arranged by size: 564 336 213 207 126 NaeI GCC'GGC Cuts at: 0 964 1446 Size: 964 482 NarI GG'CG_CC Cuts at: 0 35 1446 Size: 35 1411 NgoMIV G'CCGG_C Cuts at: 0 962 1446 Size: 962 484 NspI r_CATG'y Cuts at: 0 289 1446 Size: 289 1157 PflMI CCAn_nnn'nTGG Cuts at: 0 555 1014 1446 Size: 555 459 432 Fragments arranged by size: 555 459 432 PfoI T'CCnGG_A Cuts at: 0 608 818 872 953 1103 1446 Size: 608 210 54 81 150 343 Fragments arranged by size: 608 343 210 150 81 54 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1021 1053 1446 Size: 1021 32 393 Fragments arranged by size: 1021 393 32 PpuMI rG'GwC_Cy Cuts at: 0 152 510 1011 1446 Size: 152 358 501 435 Fragments arranged by size: 501 435 358 152 PsiI TTA'TAA Cuts at: 0 1355 1446 Size: 1355 91 PspOMI G'GGCC_C Cuts at: 0 367 1446 Size: 367 1079 PstI C_TGCA'G Cuts at: 0 626 1085 1446 Size: 626 459 361 Fragments arranged by size: 626 459 361 PvuII CAG'CTG Cuts at: 0 564 777 903 1239 1446 Size: 564 213 126 336 207 Fragments arranged by size: 564 336 213 207 126 SacI G_AGCT'C Cuts at: 0 1346 1446 Size: 1346 100 SapI GCTCTTCn'nnn_ Cuts at: 0 1334 1446 Size: 1334 112 SbfI CC_TGCA'GG Cuts at: 0 626 1446 Size: 626 820 SfcI C'TryA_G Cuts at: 0 622 1081 1446 Size: 622 459 365 Fragments arranged by size: 622 459 365 SfoI GGC'GCC Cuts at: 0 36 1446 Size: 36 1410 SmlI C'TyrA_G Cuts at: 0 1433 1446 Size: 1433 13 TatI w'GTAC_w Cuts at: 0 1226 1446 Size: 1226 220 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 304 336 1446 Size: 304 32 1110 Fragments arranged by size: 1110 304 32 Tth111I GACn'n_nGTC Cuts at: 0 1428 1446 Size: 1428 18 XmnI GAAnn'nnTTC Cuts at: 0 170 736 1446 Size: 170 566 710 Fragments arranged by size: 710 566 170 Enzymes that do cut: AarI AcuI AloI AlwNI ApaI BamHI BanI BanII BbeI BbsI BciVI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I HindIII KasI MscI MspA1I NaeI NarI NgoMIV NspI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI PvuII SacI SapI SbfI SfcI SfoI SmlI TatI TstI Tth111I XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbvCI BcgI BclI BfrBI BglII BlpI BmtI BplI BsaAI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KpnI MfeI MluI MmeI MslI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PmeI PmlI PshAI PspXI PsrI PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII XbaI XcmI XhoI XmaI ZraI