Restriction map for
pFN21AE1942
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaLI AscI Asp718I AspEI AviII
AvrII BbeI BbrPI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI PvuII RcaI
RsrII SacI SacII SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccI 136 GTCGAC GT^[AC][GT]AC
AcyI 514 GGCGTC G[GA]^CG[CT]C
AseI 1174 ATTAAT AT^TAAT
AsnI 1174 ATTAAT AT^TAAT
AspHI 1285 GAGCAC G[AT]GC[AT]^C
AspI 823 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 844 GGATCC G^GATCC
BanI 349 GGCACC G^G[CT][GA]CC
BbvCI 1254 GCTGAGG CCTCAGC(-5/-2)
BglII 1349 AGATCT A^GATCT
BsaAI 604 TACGTG [CT]AC^GT[GA]
BsaHI 514 GGCGTC G[GA]^CG[CT]C
BsiHKAI 1285 GAGCAC G[AT]GC[AT]^C
BsmAI 295 GTCTC GTCTC(1/5)
BsrFI 358 ACCGGC [GA]^CCGG[CT]
BssSI 748 CACGAG CACGAG(-5/-1)
Cfr10I 358 ACCGGC [GA]^CCGG[CT]
DrdI 589 GACGAACACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1241 CCGTGG C^C[GA][CT]GG
EcoNI 189 CCTGTCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 299 CCAAGG C^C[AT][AT]GG
Hin1I 514 GGCGTC G[GA]^CG[CT]C
MaeIII 454 GTGAC ^GT[ACGT]AC
PflFI 823 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1128 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 1174 ATTAAT AT^TAAT
SalI 136 GTCGAC G^TCGAC
ScaI 953 AGTACT AGT^ACT
StuI 653 AGGCCT AGG^CCT
StyI 299 CCAAGG C^C[AT][AT]GG
Tsp45I 454 GTGAC ^GT[GC]AC
Tth111I 823 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1128 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 353 CCATGACCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 110 CGCG CG^CG
497 CGCG
AcsI 166 GAATTT [GA]^AATT[CT]
1393 AAATTC
AfaI 954 GTAC GT^AC
1077 GTAC
AlwI 845 GATCC GGATC(4/5)
844 GGATC
ApoI 166 GAATTT [GA]^AATT[CT]
1393 AAATTC
Asp700I 180 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGAAGTTC
BalI 365 TGGCCA TGG^CCA
1463 TGGCCA
BciVI 906 GGATAC GTATCC(6/5)
329 GTATCC
BfaI 20 CTAG C^TAG
1115 CTAG
BglI 746 GCCACGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1186 GCCAAGAAGGC
BpmI 52 CTCCAG CTGGAG(16/14)
1471 CTGGAG
BsrDI 785 GCAATG GCAATG(2/0)
1235 GCAATG
BstUI 110 CGCG CG^CG
497 CGCG
BstYI 844 GGATCC [GA]^GATC[CT]
1349 AGATCT
DdeI 1255 CTGAG C^T[ACGT]AG
1269 CTCAG
FauI 285 GCGGG CCCGC(4/6)
498 GCGGG
HincII 136 GTCGAC GT[CT]^[GA]AC
247 GTCAAC
HindII 136 GTCGAC GT[CT]^[GA]AC
247 GTCAAC
HinfI 205 GAGTC G^A[ACGT]TC
1381 GAGTC
MaeI 20 CTAG C^TAG
1115 CTAG
MflI 844 GGATCC [GA]^GATC[CT]
1349 AGATCT
MluNI 365 TGGCCA TGG^CCA
1463 TGGCCA
MscI 365 TGGCCA TGG^CCA
1463 TGGCCA
MseI 1175 TTAA T^TAA
1211 TTAA
MspA1I 337 CCGCTG C[AC]G^C[GT]G
510 CAGCGG
MvnI 110 CGCG CG^CG
497 CGCG
PleI 205 GAGTC GAGTC(4/5)
1381 GAGTC
PpuMI 5 GGGTCCT [GA]G^G[AT]CC[CT]
421 AGGTCCC
RsaI 954 GTAC GT^AC
1077 GTAC
SexAI 122 ACCTGGT A^CC[AT]GGT
824 ACCTGGT
SmlI 957 CTCAAG C^T[CT][GA]AG
1072 CTCAAG
Tru9I 1175 TTAA T^TAA
1211 TTAA
XhoII 844 GGATCC [GA]^GATC[CT]
1349 AGATCT
XmnI 180 GAAGGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
882 GAAGAAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AflIII AluI AlwNI ApaI
AvaI AvaII BanII BbsI BbvI
BcnI BmyI BpuAI BsaJI BsgI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstAPI BstF5I
BstNI BstXI Cac8I CfoI Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HpaII HphI ItaI
MaeII MboI MboII MnlI MslI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PstI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI TaqI
TseI Tsp509I TspRI TthHB8I XmaCI
XmaI