(Circular) (Six-base) MAPSORT of: pFN21AE1942.seq Check: 9130 from: 1 to: 6193 pFN21AE1942 6193 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2701 2750 2701 Size: 49 6144 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4003 278 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2142 3536 1937 Size: 205 1394 4594 Fragments arranged by size: 4594 1394 205 Acc65I G'GTAC_C Cuts at: 3514 3514 Size: 6193 AclI AA'CG_TT Cuts at: 1563 4320 4693 1563 Size: 2757 373 3063 Fragments arranged by size: 3063 2757 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4256 5640 5908 1632 Size: 2624 1384 268 1917 Fragments arranged by size: 2624 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5996 AflIII A'CryG_T Cuts at: 1203 2493 2599 2922 5092 6069 1203 Size: 1290 106 323 2170 977 1327 Fragments arranged by size: 2170 1327 1290 977 323 106 AgeI A'CCGG_T Cuts at: 5024 5024 Size: 6193 AhdI GACnn_n'nnGTC Cuts at: 4922 4922 Size: 6193 AleI CACnn'nnGTG Cuts at: 1166 2240 1166 Size: 1074 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2063 3359 1184 Size: 879 1296 4018 Fragments arranged by size: 4018 1296 879 AlwNI CAG_nnn'CTG Cuts at: 2065 2344 3281 3433 3936 5508 2065 Size: 279 937 152 503 1572 2750 Fragments arranged by size: 2750 1572 937 503 279 152 ApaI G_GGCC'C Cuts at: 2229 2509 3133 2229 Size: 280 624 5289 Fragments arranged by size: 5289 624 280 ApaLI G'TGCA_C Cuts at: 4250 5406 4250 Size: 1156 5037 ApoI r'AATT_y Cuts at: 1493 2171 3398 3502 3728 3880 5989 1493 Size: 678 1227 104 226 152 2109 1697 Fragments arranged by size: 2109 1697 1227 678 226 152 104 AseI AT'TA_AT Cuts at: 160 1032 3180 4747 160 Size: 872 2148 1567 1606 Fragments arranged by size: 2148 1606 1567 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 AvaI C'yCGr_G Cuts at: 1870 1943 2238 2508 3125 3518 1870 Size: 73 295 270 617 393 4545 Fragments arranged by size: 4545 617 393 295 270 73 BamHI G'GATC_C Cuts at: 2849 3523 3890 2849 Size: 674 367 5152 Fragments arranged by size: 5152 674 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2354 3514 4969 618 Size: 325 217 627 567 1160 1455 1842 Fragments arranged by size: 1842 1455 1160 627 567 325 217 BanII G_rGCy'C Cuts at: 1391 2229 2296 2509 3133 3512 1391 Size: 838 67 213 624 379 4072 Fragments arranged by size: 4072 838 624 379 213 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2827 3142 3375 928 Size: 971 928 315 233 3746 Fragments arranged by size: 3746 971 928 315 233 BbvCI CC'TCA_GC Cuts at: 3260 3260 Size: 6193 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4384 4418 1903 Size: 34 2447 34 3678 Fragments arranged by size: 3678 2447 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2345 2905 4085 5295 2345 Size: 560 1180 1210 3243 Fragments arranged by size: 3243 1210 1180 560 BclI T'GATC_A Cuts at: 1548 1548 Size: 6193 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2757 3197 4804 Size: 107 122 71 631 605 1085 440 1607 Cuts at: 4804 136 Size: 1525 Fragments arranged by size: 1607 1525 1085 631 605 440 122 107 71 BglII A'GATC_T Cuts at: 3354 6188 3354 Size: 2834 3359 BlpI GC'TnA_GC Cuts at: 3610 3610 Size: 6193 Bme1580I G_kGCm'C Cuts at: 1790 2229 2509 3059 3133 4254 5410 1790 Size: 439 280 550 74 1121 1156 2573 Fragments arranged by size: 2573 1156 1121 550 439 280 74 BmgBI CAC'GTC Cuts at: 1318 2221 2494 2602 2923 1318 Size: 903 273 108 321 4588 Fragments arranged by size: 4588 903 321 273 108 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2083 3075 3924 Size: 416 53 83 399 24 666 992 849 Cuts at: 3924 4882 442 Size: 958 1753 Fragments arranged by size: 1753 992 958 849 666 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6193 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2120 2120 Size: 6193 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2042 3497 4853 1239 Size: 803 1455 1356 2579 Fragments arranged by size: 2579 1455 1356 803 Bpu10I CC'TnA_GC Cuts at: 3260 3260 Size: 6193 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2947 3062 4318 5183 5481 2947 Size: 115 1256 865 298 3659 Fragments arranged by size: 3659 1256 865 298 115 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4856 882 Size: 617 3357 2219 Fragments arranged by size: 3357 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2611 3984 493 Size: 711 1407 1373 2702 Fragments arranged by size: 2702 1407 1373 711 BsaBI GATnn'nnATC Cuts at: 6187 6187 Size: 6193 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2520 4000 4382 5017 Size: 53 83 186 936 987 1480 382 635 Cuts at: 5017 275 Size: 1451 Fragments arranged by size: 1480 1451 987 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4626 5024 5298 5445 5856 4626 Size: 398 274 147 411 4963 Fragments arranged by size: 4963 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5968 Fragments arranged by size: 5968 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6193 BseYI C'CCAG_C Cuts at: 5396 5892 5396 Size: 496 5697 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2089 2507 2975 1163 Size: 573 353 418 468 4381 Fragments arranged by size: 4381 573 468 418 353 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3643 4404 5432 1540 Size: 275 186 1642 761 1028 2301 Fragments arranged by size: 2301 1642 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3294 4254 4339 5410 3294 Size: 960 85 1071 4077 Fragments arranged by size: 4077 1071 960 85 BsmI GAATG_Cn' Cuts at: 3704 3797 3704 Size: 93 6100 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2296 2509 3059 3133 3294 3512 Size: 399 439 67 213 550 74 161 218 Cuts at: 3512 4254 4339 5410 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 550 439 399 218 213 161 85 74 67 BspEI T'CCGG_A Cuts at: 5856 5856 Size: 6193 BspHI T'CATG_A Cuts at: 4082 5812 4082 Size: 1730 4463 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2701 2750 2795 3548 844 Size: 1857 49 45 753 3489 Fragments arranged by size: 3489 1857 753 49 45 BsrBI CCG'CTC Cuts at: 1394 1823 4080 1394 Size: 429 2257 3507 Fragments arranged by size: 3507 2257 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2797 3247 4688 4862 65 Size: 1183 1549 450 1441 174 1396 Fragments arranged by size: 1549 1441 1396 1183 450 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2363 4837 5024 1700 Size: 110 553 2474 187 2869 Fragments arranged by size: 2869 2474 553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6193 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6193 BssSI C'ACGA_G Cuts at: 2753 4253 5265 2753 Size: 1500 1012 3681 Fragments arranged by size: 3681 1500 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 2787 2796 794 Size: 1532 461 9 4191 Fragments arranged by size: 4191 1532 461 9 BstBI TT'CG_AA Cuts at: 4997 4997 Size: 6193 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6193 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2242 2542 2830 3445 2128 Size: 114 300 288 615 4876 Fragments arranged by size: 4876 615 300 288 114 BstYI r'GATC_y Cuts at: 1972 2849 3354 3523 3890 4275 4292 5744 5830 Size: 877 505 169 367 385 17 1452 86 Cuts at: 5830 5842 6188 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 877 505 385 367 346 169 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3246 513 Size: 552 2181 3460 Fragments arranged by size: 3460 2181 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2418 487 Size: 683 56 1192 4262 Fragments arranged by size: 4262 1192 683 56 BtsI GCAGTG_nn' Cuts at: 2312 2806 3709 4503 4523 2312 Size: 494 903 794 20 3982 Fragments arranged by size: 3982 903 794 494 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6193 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6158 DraI TTT'AAA Cuts at: 3497 3497 Size: 6193 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2600 5200 809 Size: 507 1284 2600 1802 Fragments arranged by size: 2600 1802 1284 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2370 2820 3160 Size: 54 1425 50 275 18 540 450 340 Cuts at: 3160 3468 3640 4529 6158 8 Size: 308 172 889 1629 43 Fragments arranged by size: 1629 1425 889 540 450 340 308 275 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3640 1537 Size: 275 1828 4090 Fragments arranged by size: 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4123 1353 Size: 2770 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4780 5154 5300 387 Size: 1064 138 3191 374 146 1280 Fragments arranged by size: 3191 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2042 3497 4256 4853 5640 5908 1239 Size: 393 410 1455 759 597 787 268 1524 Fragments arranged by size: 1524 1455 787 759 597 410 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2198 2198 Size: 6193 EcoO109I rG'GnC_Cy Cuts at: 2011 2062 2225 2427 2472 3129 3430 2011 Size: 51 163 202 45 657 301 4774 Fragments arranged by size: 4774 657 301 202 163 51 45 EcoRI G'AATT_C Cuts at: 3502 3880 3502 Size: 378 5815 FspI TGC'GCA Cuts at: 4699 4699 Size: 6193 HaeII r_GCGC'y Cuts at: 1678 5340 1678 Size: 3662 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2624 2656 4834 4866 4908 Size: 32 135 32 1052 32 2178 32 42 Cuts at: 4908 4940 1373 Size: 32 2626 Fragments arranged by size: 2626 2178 1052 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2143 2254 3537 3783 669 Size: 988 281 205 111 1283 246 3079 Fragments arranged by size: 3079 1283 988 281 246 205 111 HindIII A'AGCT_T Cuts at: 748 748 Size: 6193 HpaI GTT'AAC Cuts at: 3783 3783 Size: 6193 KpnI G_GTAC'C Cuts at: 3518 3518 Size: 6193 MfeI C'AATT_G Cuts at: 3792 3792 Size: 6193 MluI A'CGCG_T Cuts at: 6069 6069 Size: 6193 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5307 5491 1305 Size: 4002 184 2007 Fragments arranged by size: 4002 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2372 3470 10 Size: 54 1425 343 540 1098 2733 Fragments arranged by size: 2733 1425 1098 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2240 2456 2540 2703 4151 4510 4669 Size: 648 1074 216 84 163 1448 359 159 Cuts at: 4669 518 Size: 2042 Fragments arranged by size: 2042 1448 1074 648 359 216 163 159 84 MspA1I CmG'CkG Cuts at: 1606 2344 2517 3609 4286 5434 5679 1606 Size: 738 173 1092 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 738 677 245 173 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5641 NdeI CA'TA_TG Cuts at: 387 387 Size: 6193 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6193 NotI GC'GGCC_GC Cuts at: 3640 3640 Size: 6193 NspI r_CATG'y Cuts at: 1132 1229 3551 5096 1132 Size: 97 2322 1545 2229 Fragments arranged by size: 2322 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2989 1348 Size: 1641 4552 PciI A'CATG_T Cuts at: 5092 5092 Size: 6193 PflMI CCAn_nnn'nTGG Cuts at: 1108 3139 1108 Size: 2031 4162 PfoI T'CCnGG_A Cuts at: 2642 2652 3474 2642 Size: 10 822 5361 Fragments arranged by size: 5361 822 10 PmeI GTTT'AAAC Cuts at: 3497 3497 Size: 6193 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4262 4294 5775 5807 4262 Size: 32 1481 32 4648 Fragments arranged by size: 4648 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 2427 2011 Size: 416 5777 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6193 PsiI TTA'TAA Cuts at: 3763 3763 Size: 6193 PspOMI G'GGCC_C Cuts at: 2225 2505 3129 2225 Size: 280 624 5289 Fragments arranged by size: 5289 624 280 PstI C_TGCA'G Cuts at: 830 2042 2322 2801 3429 3545 830 Size: 1212 280 479 628 116 3478 Fragments arranged by size: 3478 1212 628 479 280 116 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6193 SalI G'TCGA_C Cuts at: 1936 2141 3535 1936 Size: 205 1394 4594 Fragments arranged by size: 4594 1394 205 SbfI CC_TGCA'GG Cuts at: 3545 3545 Size: 6193 ScaI AGT'ACT Cuts at: 2960 4441 6153 2960 Size: 1481 1712 3000 Fragments arranged by size: 3000 1712 1481 SexAI A'CCwGG_T Cuts at: 2127 2829 2127 Size: 702 5491 SfcI C'TryA_G Cuts at: 826 1045 2038 2318 2620 2797 3425 3541 4676 Size: 219 993 280 302 177 628 116 1135 Cuts at: 4676 5357 5548 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 993 681 628 302 280 219 191 177 116 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6193 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6193 SmaI CCC'GGG Cuts at: 1872 2240 2510 3127 3520 1872 Size: 368 270 617 393 4545 Fragments arranged by size: 4545 617 393 368 270 SmlI C'TyrA_G Cuts at: 820 1017 1943 2962 3077 4297 5198 5460 820 Size: 197 926 1019 115 1220 901 262 1553 Fragments arranged by size: 1553 1220 1019 926 901 262 197 115 SnaBI TAC'GTA Cuts at: 493 3984 493 Size: 3491 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6193 SphI G_CATG'C Cuts at: 1132 3551 1132 Size: 2419 3774 SrfI GCCC'GGGC Cuts at: 2510 2510 Size: 6193 SspI AAT'ATT Cuts at: 5 52 4117 5 Size: 47 4065 2081 Fragments arranged by size: 4065 2081 47 StuI AGG'CCT Cuts at: 1517 2660 1517 Size: 1143 5050 StyI C'CwwG_G Cuts at: 513 1065 2304 3632 513 Size: 552 1239 1328 3074 Fragments arranged by size: 3074 1328 1239 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3221 4220 4237 4390 4575 5020 325 Size: 1255 1641 999 17 153 185 445 1498 Fragments arranged by size: 1641 1498 1255 999 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2958 4439 6151 Size: 274 80 33 51 1443 981 1481 1712 Cuts at: 6151 96 Size: 138 Fragments arranged by size: 1712 1481 1443 981 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3359 3391 6153 6185 3359 Size: 32 2762 32 3367 Fragments arranged by size: 3367 2762 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2831 1316 Size: 1515 4678 XbaI T'CTAG_A Cuts at: 3529 3529 Size: 6193 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2365 2365 Size: 6193 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6193 XmaI C'CCGG_G Cuts at: 1870 2238 2508 3125 3518 1870 Size: 368 270 617 393 4545 Fragments arranged by size: 4545 617 393 368 270 XmnI GAAnn'nnTTC Cuts at: 2189 2891 4322 2189 Size: 702 1431 4060 Fragments arranged by size: 4060 1431 702 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4001 276 Size: 53 83 186 936 2467 2468 Fragments arranged by size: 2468 2467 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SwaI