################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FbaI FseI FspI Hin1I HindIII HinfI HpaI HphI KasI KpnI KspI MaeI MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacI SacII SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccI 97 GTCGAC GT^[AC][GT]AC AluI 204 AGCT AG^CT AlwI 81 GGATC GGATC(4/5) Asp700I 229 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 179 GAGCAC G[AT]GC[AT]^C AvaI 65 CCCGAG C^[CT]CG[GA]G AvaII 53 GGACC G^G[AT]CC BanII 111 GGGCTC G[GA]GC[CT]^C BbvI 202 GCAGC GCAGC(8/12) BglI 127 GCCGGTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsiEI 99 CGACCG CG[GA][CT]^CG BsiHKAI 179 GAGCAC G[AT]GC[AT]^C BsiYI 102 CCGGGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 102 CCGGGGCGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 65 CCCGAG C^[CT]CG[GA]G BsrFI 127 GCCGGT [GA]^CCGG[CT] BsrI 198 ACTGG ACTGG(1/-1) BstF5I 117 CATCC GGATG(2/0) Cfr10I 127 GCCGGT [GA]^CCGG[CT] DraII 133 GGGGCCT [GA]G^G[ACGT]CC[CT] EarI 160 GAAGAG CTCTTC(1/4) EcoO109I 133 GGGGCCT [GA]G^G[ACGT]CC[CT] FauI 109 GCGGG CCCGC(4/6) FokI 117 CATCC GGATG(9/13) HaeII 278 AGCGCC [GA]GCGC^[CT] HaeIII 135 GGCC GG^CC HgaI 258 GCGTC GACGC(5/10) HincII 97 GTCGAC GT[CT]^[GA]AC HindII 97 GTCGAC GT[CT]^[GA]AC Ksp632I 160 GAAGAG CTCTTC(1/4) MaeII 28 ACGT A^CGT MaeIII 7 GTGAC ^GT[ACGT]AC PstI 262 CTGCAG CTGCA^G SalI 97 GTCGAC G^TCGAC SfcI 262 CTGCAG C^T[GA][CT]AG TaqI 98 TCGA T^CGA TseI 202 GCAGC G^C[AT]GC Tsp45I 7 GTGAC ^GT[GC]AC Tsp509I 273 AATT ^AATT TspRI 5 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 98 TCGA T^CGA XmnI 229 GAAATCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccII 23 CGCG CG^CG 108 CGCG BcnI 102 CCGGG CC^[GC]GG 120 CCGGG BmyI 111 GGGCTC G[AGT]GC[ACT]^C 179 GAGCAC BsaJI 66 CCGAGG C^C[ACGT][ACGT]GG 102 CCGGGG Bsp1286I 111 GGGCTC G[AGT]GC[ACT]^C 179 GAGCAC BssKI 102 CCGGG ^CC[ACGT]GG 120 CCGGG BstUI 23 CGCG CG^CG 108 CGCG Cfr13I 53 GGACC G^G[ACGT]CC 134 GGGCC DpnII 82 GATC ^GATC 294 GATC Fnu4HI 19 GCGGC GC^[ACGT]GC 202 GCAGC ItaI 19 GCGGC GC^[ACGT]GC 202 GCAGC MboI 82 GATC ^GATC 294 GATC MvnI 23 CGCG CG^CG 108 CGCG NciI 102 CCGGG CC^[GC]GG 120 CCGGG NdeII 82 GATC ^GATC 294 GATC NlaIV 112 GGCTCC GG[ACGT]^[ACGT]CC 133 GGGGCC Sau3AI 82 GATC ^GATC 294 GATC Sau96I 53 GGACC G^G[ACGT]CC 134 GGGCC ScrFI 102 CCGGG CC^[ACGT]GG 120 CCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 12 enzymes) # ######################################### AciI Cac8I CfoI HapII HhaI HinP1I HpaII MboII MnlI MspI MwoI NlaIII