(Linear) (Six-base) MAPSORT of: KIEE5153.seq  Check: 478  from: 1  to: 318



KIEE5153.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:48  ..

AccI GT'mk_AC

Cuts at:      0      98     318 
   Size:         98     220
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     318 
   Size:        179     139
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     158     190     318 
   Size:        158      32     128

  Fragments arranged by size: 

                 158     128      32
 
AvaI C'yCGr_G

Cuts at:      0      65     318 
   Size:         65     253
 
BanII G_rGCy'C

Cuts at:      0     115     318 
   Size:        115     203
 
BglI GCCn_nnn'nGGC

Cuts at:      0     133     318 
   Size:        133     185
 
BmgBI CAC'GTC

Cuts at:      0      29     318 
   Size:         29     289
 
BsiEI CG_ry'CG

Cuts at:      0     102     318 
   Size:        102     216
 
BsiHKAI G_wGCw'C

Cuts at:      0     183     318 
   Size:        183     135
 
Bsp1286I G_dGCh'C

Cuts at:      0     115     183     318 
   Size:        115      68     135

  Fragments arranged by size: 

                 135     115      68
 
BsrFI r'CCGG_y

Cuts at:      0     127     318 
   Size:        127     191
 
BtsI GCAGTG_nn'

Cuts at:      0      11     318 
   Size:         11     307
 
EarI CTCTTCn'nnn_

Cuts at:      0     155     318 
   Size:        155     163
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     179     318 
   Size:        179     139
 
EcoO109I rG'GnC_Cy

Cuts at:      0     134     318 
   Size:        134     184
 
HaeII r_GCGC'y

Cuts at:      0     282     318 
   Size:        282      36
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     243     275     318 
   Size:        243      32      43

  Fragments arranged by size: 

                 243      43      32
 
HincII GTy'rAC

Cuts at:      0      99     318 
   Size:         99     219
 
PfoI T'CCnGG_A

Cuts at:      0     119     318 
   Size:        119     199
 
PstI C_TGCA'G

Cuts at:      0     266     318 
   Size:        266      52
 
SalI G'TCGA_C

Cuts at:      0      97     318 
   Size:         97     221
 
SfcI C'TryA_G

Cuts at:      0     262     318 
   Size:        262      56
 
XmnI GAAnn'nnTTC

Cuts at:      0     233     318 
   Size:        233      85
 

 Enzymes that do cut:

     AccI     AcuI     AloI     AvaI    BanII     BglI    BmgBI    BsiEI
  BsiHKAI Bsp1286I    BsrFI     BtsI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I   HincII     PfoI     PstI     SalI     SfcI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI