Restriction map for
pFN21AB9941
(Circular) (Six-base) MAPSORT of: pFN21AB9941.seq Check: 9576 from: 1 to: 6292
pFN21AB9941 6292 bp
With 182 enzymes: SgfI *
November 18, 2016 17:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4102 278
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3635 1937
Size: 1698 4594
Acc65I G'GTAC_C
Cuts at: 3613 3613
Size: 6292
AclI AA'CG_TT
Cuts at: 1563 4419 4792 1563
Size: 2856 373 3063
Fragments arranged by size:
3063 2856 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2062 2355 2448 2903 4355 5739 6007 1632
Size: 430 293 93 455 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 455 430 293 268 93
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6095
AflIII A'CryG_T
Cuts at: 1203 3324 5191 6168 1203
Size: 2121 1867 977 1327
Fragments arranged by size:
2121 1867 1327 977
AgeI A'CCGG_T
Cuts at: 5123 5123
Size: 6292
AhdI GACnn_n'nnGTC
Cuts at: 3328 3489 5021 3328
Size: 161 1532 4599
Fragments arranged by size:
4599 1532 161
AleI CACnn'nnGTG
Cuts at: 1166 2864 1166
Size: 1698 4594
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6292
AlwNI CAG_nnn'CTG
Cuts at: 2668 2749 3290 4035 5607 2668
Size: 81 541 745 1572 3353
Fragments arranged by size:
3353 1572 745 541 81
ApaLI G'TGCA_C
Cuts at: 4349 5505 4349
Size: 1156 5136
ApoI r'AATT_y
Cuts at: 1493 3601 3827 3979 6088 1493
Size: 2108 226 152 2109 1697
Fragments arranged by size:
2109 2108 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4846 160
Size: 872 3814 1606
Fragments arranged by size:
3814 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
AvaI C'yCGr_G
Cuts at: 1870 1943 3578 3617 1870
Size: 73 1635 39 4545
Fragments arranged by size:
4545 1635 73 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3554 3587 3554
Size: 33 6259
BamHI G'GATC_C
Cuts at: 3339 3622 3989 3339
Size: 283 367 5642
Fragments arranged by size:
5642 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2210 2537 2813 2891 3613
Size: 325 217 627 423 327 276 78 722
Cuts at: 3613 5068 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 722 627 423 327 325 276
217 78
BanII G_rGCy'C
Cuts at: 1391 2991 3357 3611 1391
Size: 1600 366 254 4072
Fragments arranged by size:
4072 1600 366 254
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2882 928
Size: 971 983 4338
Fragments arranged by size:
4338 983 971
BbvCI CC'TCA_GC
Cuts at: 3105 3105
Size: 6292
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4483 4517 1903
Size: 34 2546 34 3678
Fragments arranged by size:
3678 2546 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4184 5394 4184
Size: 1210 5082
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6292
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2811 3447 4903
Size: 107 122 71 631 605 1139 636 1456
Cuts at: 4903 136
Size: 1525
Fragments arranged by size:
1525 1456 1139 636 631 605 122 107
71
BglII A'GATC_T
Cuts at: 6287 6287
Size: 6292
BlpI GC'TnA_GC
Cuts at: 3709 3709
Size: 6292
Bme1580I G_kGCm'C
Cuts at: 1790 2816 2846 2894 3443 4353 5509 1790
Size: 1026 30 48 549 910 1156 2573
Fragments arranged by size:
2573 1156 1026 910 549 48 30
BmgBI CAC'GTC
Cuts at: 1318 3325 1318
Size: 2007 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2572 4023 4981
Size: 416 53 83 399 24 1155 1451 958
Cuts at: 4981 442
Size: 1753
Fragments arranged by size:
1753 1451 1155 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2819 2936 3429 3560 4952 1239
Size: 1580 117 493 131 1392 2579
Fragments arranged by size:
2579 1580 1392 493 131 117
Bpu10I CC'TnA_GC
Cuts at: 3105 3105
Size: 6292
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3026 4417 5282 5580 3026
Size: 1391 865 298 3738
Fragments arranged by size:
3738 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2364 2519 4955 882
Size: 617 865 155 2436 2219
Fragments arranged by size:
2436 2219 865 617 155
BsaAI yAC'GTr
Cuts at: 493 1204 2401 2847 4083 493
Size: 711 1197 446 1236 2702
Fragments arranged by size:
2702 1236 1197 711 446
BsaBI GATnn'nnATC
Cuts at: 6286 6286
Size: 6292
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2766 3490 4099 4481
Size: 53 83 186 936 1233 724 609 382
Cuts at: 4481 5116 275
Size: 635 1451
Fragments arranged by size:
1451 1233 936 724 635 609 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2369 4725 5123 5397 5544 5955 2369
Size: 2356 398 274 147 411 2706
Fragments arranged by size:
2706 2356 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2342 2372 3539 3569 1178
Size: 30 165 30 939 30 1167 30 3901
Fragments arranged by size:
3901 1167 939 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2164 2933 2999 3518 1186
Size: 978 769 66 519 3960
Fragments arranged by size:
3960 978 769 519 66
BseYI C'CCAG_C
Cuts at: 2390 2749 2809 2839 3454 5495 5991 2390
Size: 359 60 30 615 2041 496 2691
Fragments arranged by size:
2691 2041 615 496 359 60 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2681 2693 2710 2981 1163
Size: 573 945 12 17 271 4474
Fragments arranged by size:
4474 945 573 271 17 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3742 4503 5531 1540
Size: 275 186 1741 761 1028 2301
Fragments arranged by size:
2301 1741 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3357 4353 4438 5509 3357
Size: 996 85 1071 4140
Fragments arranged by size:
4140 1071 996 85
BsmI GAATG_Cn'
Cuts at: 2385 3803 3896 2385
Size: 1418 93 4781
Fragments arranged by size:
4781 1418 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3224 3224
Size: 6292
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2816 2846 2894 2991 3357 3443 3611
Size: 399 1026 30 48 97 366 86 168
Cuts at: 3611 4353 4438 5509 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1026 742 399 366 168 97
86 85 48 30
BspEI T'CCGG_A
Cuts at: 2369 5955 2369
Size: 3586 2706
BspHI T'CATG_A
Cuts at: 2733 2931 4181 5911 2733
Size: 198 1250 1730 3114
Fragments arranged by size:
3114 1730 1250 198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2718 2917 3534 3647 844
Size: 1874 199 617 113 3489
Fragments arranged by size:
3489 1874 617 199 113
BsrBI CCG'CTC
Cuts at: 1394 1823 3181 4179 1394
Size: 429 1358 998 3507
Fragments arranged by size:
3507 1358 998 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2250 4787 4961 65
Size: 1183 1002 2537 174 1396
Fragments arranged by size:
2537 1396 1183 1002 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2035 2805 2816 4936 5123 1700
Size: 110 225 770 11 2120 187 2869
Fragments arranged by size:
2869 2120 770 225 187 110 11
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6292
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6292
BssSI C'ACGA_G
Cuts at: 4352 5364 4352
Size: 1012 5280
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2820 794
Size: 2026 4266
BstBI TT'CG_AA
Cuts at: 5096 5096
Size: 6292
BstEII G'GTnAC_C
Cuts at: 1184 2239 1184
Size: 1055 5237
BstXI CCAn_nnnn'nTGG
Cuts at: 2797 3169 2797
Size: 372 5920
BstYI r'GATC_y
Cuts at: 1972 2601 3339 3622 3989 4374 4391 5843 5929
Size: 629 738 283 367 385 17 1452 86
Cuts at: 5929 5941 6287 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 738 629 385 367 346 283
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3152 3152
Size: 6292
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 2973 3474 513
Size: 552 939 39 930 501 3331
Fragments arranged by size:
3331 939 930 552 501 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2701 487
Size: 683 56 1475 4078
Fragments arranged by size:
4078 1475 683 56
BtsI GCAGTG_nn'
Cuts at: 3808 4602 4622 3808
Size: 794 20 5478
Fragments arranged by size:
5478 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6292
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6257
DraI TTT'AAA
Cuts at: 3596 3596
Size: 6292
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5299 809
Size: 507 3983 1802
Fragments arranged by size:
3983 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2040 2046 2757
Size: 54 1425 50 275 18 210 6 711
Cuts at: 2757 3739 4628 6257 8
Size: 982 889 1629 43
Fragments arranged by size:
1629 1425 982 889 711 275 210 54
50 43 18 6
EagI C'GGCC_G
Cuts at: 1537 1812 3739 1537
Size: 275 1927 4090
Fragments arranged by size:
4090 1927 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2424 4222 1353
Size: 1071 1798 3423
Fragments arranged by size:
3423 1798 1071
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4879 5253 5399 387
Size: 1064 138 3290 374 146 1280
Fragments arranged by size:
3290 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3355 3355
Size: 6292
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2062 2355 2448 2819 2903 2936 3429
Size: 393 430 293 93 371 84 33 493
Cuts at: 3429 3560 4355 4952 5739 6007 1239
Size: 131 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 493 430 393 371
293 268 131 93 84 33
EcoNI CCTnn'n_nnAGG
Cuts at: 2759 2918 3467 2759
Size: 159 549 5584
Fragments arranged by size:
5584 549 159
EcoO109I rG'GnC_Cy
Cuts at: 2502 2502
Size: 6292
EcoRI G'AATT_C
Cuts at: 3601 3979 3601
Size: 378 5914
FspI TGC'GCA
Cuts at: 4798 4798
Size: 6292
HaeII r_GCGC'y
Cuts at: 1678 2806 5439 1678
Size: 1128 2633 2531
Fragments arranged by size:
2633 2531 1128
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2342 2374 3297 3314 3329
Size: 32 135 32 770 32 923 17 15
Cuts at: 3329 3346 3475 3507 4933 4965 5007 5039 1373
Size: 17 129 32 1426 32 42 32 2626
Fragments arranged by size:
2626 1426 923 770 135 129 42 32
32 32 32 32 32 17 17 15
HincII GTy'rAC
Cuts at: 669 1657 1938 2413 3636 3882 669
Size: 988 281 475 1223 246 3079
Fragments arranged by size:
3079 1223 988 475 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6292
HpaI GTT'AAC
Cuts at: 3882 3882
Size: 6292
KpnI G_GTAC'C
Cuts at: 3617 3617
Size: 6292
MfeI C'AATT_G
Cuts at: 3077 3891 3077
Size: 814 5478
MluI A'CGCG_T
Cuts at: 6168 6168
Size: 6292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2961 3387 5406 5590 1305
Size: 1656 426 2019 184 2007
Fragments arranged by size:
2019 2007 1656 426 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2042 2759 10
Size: 54 1425 343 210 717 3543
Fragments arranged by size:
3543 1425 717 343 210 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 2732 2864 4250 4609 4768 518
Size: 648 1521 45 132 1386 359 159 2042
Fragments arranged by size:
2042 1521 1386 648 359 159 132 45
MspA1I CmG'CkG
Cuts at: 1606 2476 2965 3560 3708 4385 5533 5778 1606
Size: 870 489 595 148 677 1148 245 2120
Fragments arranged by size:
2120 1148 870 677 595 489 245 148
NaeI GCC'GGC
Cuts at: 2807 2807
Size: 6292
NcoI C'CATG_G
Cuts at: 513 1065 2004 3474 513
Size: 552 939 1470 3331
Fragments arranged by size:
3331 1470 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6292
NgoMIV G'CCGG_C
Cuts at: 2805 2805
Size: 6292
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6292
NotI GC'GGCC_GC
Cuts at: 3739 3739
Size: 6292
NspI r_CATG'y
Cuts at: 1132 1229 3436 3650 5195 1132
Size: 97 2207 214 1545 2229
Fragments arranged by size:
2229 2207 1545 214 97
PasI CC'CwG_GG
Cuts at: 1348 2497 1348
Size: 1149 5143
PciI A'CATG_T
Cuts at: 5191 5191
Size: 6292
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3082 3273 1108
Size: 1974 191 4127
Fragments arranged by size:
4127 1974 191
PfoI T'CCnGG_A
Cuts at: 2556 2913 3537 2556
Size: 357 624 5311
Fragments arranged by size:
5311 624 357
PmeI GTTT'AAAC
Cuts at: 3596 3596
Size: 6292
PmlI CAC'GTG
Cuts at: 2847 2847
Size: 6292
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4361 4393 5874 5906 4361
Size: 32 1481 32 4747
Fragments arranged by size:
4747 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6292
PsiI TTA'TAA
Cuts at: 3862 3862
Size: 6292
PstI C_TGCA'G
Cuts at: 830 3531 3644 830
Size: 2701 113 3478
Fragments arranged by size:
3478 2701 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6292
PvuII CAG'CTG
Cuts at: 2476 2965 2476
Size: 489 5803
SacI G_AGCT'C
Cuts at: 3357 3357
Size: 6292
SalI G'TCGA_C
Cuts at: 1936 3634 1936
Size: 1698 4594
SbfI CC_TGCA'GG
Cuts at: 3644 3644
Size: 6292
ScaI AGT'ACT
Cuts at: 4540 6252 4540
Size: 1712 4580
SfcI C'TryA_G
Cuts at: 826 1045 2161 3527 3640 4775 5456 5647 826
Size: 219 1116 1366 113 1135 681 191 1471
Fragments arranged by size:
1471 1366 1135 1116 681 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6292
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6292
SmaI CCC'GGG
Cuts at: 1872 3580 3619 1872
Size: 1708 39 4545
Fragments arranged by size:
4545 1708 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3041 4396 5297 5559 820
Size: 197 926 1098 1355 901 262 1553
Fragments arranged by size:
1553 1355 1098 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4083 493
Size: 3590 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6292
SphI G_CATG'C
Cuts at: 1132 3650 1132
Size: 2518 3774
SspI AAT'ATT
Cuts at: 5 52 4216 5
Size: 47 4164 2081
Fragments arranged by size:
4164 2081 47
StuI AGG'CCT
Cuts at: 1517 2909 3511 1517
Size: 1392 602 4298
Fragments arranged by size:
4298 1392 602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2049 2301 2760 3250 3363 3474
Size: 552 939 45 252 459 490 113 111
Cuts at: 3474 3731 513
Size: 257 3074
Fragments arranged by size:
3074 939 552 490 459 257 252 113
111 45
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2346 3020 4319 4336 4489 4674 5119
Size: 1255 766 674 1299 17 153 185 445
Cuts at: 5119 325
Size: 1498
Fragments arranged by size:
1498 1299 1255 766 674 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3237 4538 6250
Size: 274 80 33 51 1443 1260 1301 1712
Cuts at: 6250 96
Size: 138
Fragments arranged by size:
1712 1443 1301 1260 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6252 6284 6252
Size: 32 6260
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6292
XbaI T'CTAG_A
Cuts at: 3628 3628
Size: 6292
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2398 2398
Size: 6292
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6292
XmaI C'CCGG_G
Cuts at: 1870 3578 3617 1870
Size: 1708 39 4545
Fragments arranged by size:
4545 1708 39
XmnI GAAnn'nnTTC
Cuts at: 2909 4421 2909
Size: 1512 4780
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4100 276
Size: 53 83 186 936 2566 2468
Fragments arranged by size:
2566 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI
PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI
PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI