Restriction map for
pFN21AE3143
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# NO CUT ENZYMES ( 140 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanII BbeI BbvCI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CfoI Cfr10I ClaI CpoI DraI
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HincII HindII HpaI
KasI KpnI KspI MaeI MfeI
MluI MluNI MroI MscI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AcsI 994 GAATTC [GA]^AATT[CT]
AcyI 949 GACGCC G[GA]^CG[CT]C
AlwNI 793 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 994 GAATTC [GA]^AATT[CT]
AvaII 176 GGACC G^G[AT]CC
BamHI 446 GGATCC G^GATCC
BanI 414 GGTGCC G^G[CT][GA]CC
BbrPI 405 CACGTG CAC^GTG
BclI 245 TGATCA T^GATCA
BcnI 183 CCGGG CC^[GC]GG
BsaAI 405 CACGTG [CT]AC^GT[GA]
BsaBI 28 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 949 GACGCC G[GA]^CG[CT]C
BsgI 550 GTGCAG GTGCAG(16/14)
BsiEI 334 CGGCCG CG[GA][CT]^CG
BsmAI 401 GTCTC GTCTC(1/5)
BsrDI 473 CATTGC GCAATG(2/0)
BssSI 238 CACGAG CACGAG(-5/-1)
BstYI 446 GGATCC [GA]^GATC[CT]
DraII 728 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 38 CCATGG C^C[GA][CT]GG
EaeI 334 CGGCCG [CT]^GGCC[GA]
EagI 334 CGGCCG C^GGCCG
EclXI 334 CGGCCG C^GGCCG
Eco52I 334 CGGCCG C^GGCCG
EcoNI 18 CCTGATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 728 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRI 994 GAATTC G^AATTC
FauI 535 GCGGG CCCGC(4/6)
FbaI 245 TGATCA T^GATCA
HgaI 949 GACGC GACGC(5/10)
Hin1I 949 GACGCC G[GA]^CG[CT]C
MamI 28 GATGGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 446 GGATCC [GA]^GATC[CT]
NciI 183 CCGGG CC^[GC]GG
NcoI 38 CCATGG C^CATGG
PflMI 792 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1027 GAGTC GAGTC(4/5)
PmaCI 405 CACGTG CAC^GTG
PmlI 405 CACGTG CAC^GTG
SapI 619 GAAGAGC GCTCTTC(1/4)
SexAI 608 ACCAGGT A^CC[AT]GGT
StuI 455 AGGCCT AGG^CCT
Tsp509I 995 AATT ^AATT
Van91I 792 CCAGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 446 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AfaI 267 GTAC GT^AC
744 GTAC
AflIII 406 ACGTGT A^C[GA][CT]GT
641 ACACGT
BbsI 888 GAAGAC GAAGAC(2/6)
484 GTCTTC
BlpI 624 GCTGAGC GC^T[ACGT]AGC
714 GCTTAGC
Bpu1102I 624 GCTGAGC GC^T[ACGT]AGC
714 GCTTAGC
BpuAI 888 GAAGAC GAAGAC(2/6)
484 GTCTTC
CelII 624 GCTGAGC GC^T[ACGT]AGC
714 GCTTAGC
DraIII 238 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
259 CACGGAGTG
HapII 132 CCGG C^CGG
183 CCGG
HindIII 712 AAGCTT A^AGCTT
863 AAGCTT
HpaII 132 CCGG C^CGG
183 CCGG
MaeII 406 ACGT A^CGT
643 ACGT
MslI 373 CACGAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
525 CATGCTGGTG
MspI 132 CCGG C^CGG
183 CCGG
RsaI 267 GTAC GT^AC
744 GTAC
TfiI 33 GAATC G^A[AT]TC
577 GATTC
TspRI 98 CAGTG CA[GC]TG[ACGT][ACGT]^
194 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AluI AlwI AspHI BbvI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstXI Cac8I
Cfr13I DdeI DpnII EarI EcoRII
EcoT14I Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PstI PvuII
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TseI Tsp45I TthHB8I XcmI