(Circular) (Six-base) MAPSORT of: pFN21AE3143.seq Check: 4063 from: 1 to: 5764 pFN21AE3143 5764 bp With 182 enzymes: SgfI * December 28, 2011 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2243 2435 2243 Size: 192 5572 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3107 1937 Size: 1170 4594 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3827 5211 5479 1632 Size: 2195 1384 268 1917 Fragments arranged by size: 2195 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 2411 2646 4663 5640 1203 Size: 1208 235 2017 977 1327 Fragments arranged by size: 2017 1327 1208 977 235 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5764 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AlwNI CAG_nnn'CTG Cuts at: 2803 3507 5079 2803 Size: 704 1572 3488 Fragments arranged by size: 3488 1572 704 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 2999 3073 3299 3451 5560 1493 Size: 1506 74 226 152 2109 1697 Fragments arranged by size: 2109 1697 1506 226 152 74 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3089 1870 Size: 73 1146 4545 Fragments arranged by size: 4545 1146 73 BamHI G'GATC_C Cuts at: 2451 3094 3461 2451 Size: 643 367 4754 Fragments arranged by size: 4754 643 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2419 3085 4540 618 Size: 325 217 627 632 666 1455 1842 Fragments arranged by size: 1842 1455 666 632 627 325 217 BanII G_rGCy'C Cuts at: 1391 3083 1391 Size: 1692 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2482 2900 928 Size: 971 583 418 3792 Fragments arranged by size: 3792 971 583 418 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3656 4866 3656 Size: 1210 4554 BclI T'GATC_A Cuts at: 1548 2250 1548 Size: 702 5062 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 2630 2720 3181 2630 Size: 90 461 5213 Fragments arranged by size: 5213 461 90 Bme1580I G_kGCm'C Cuts at: 1790 2424 3825 4981 1790 Size: 634 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 634 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3495 4453 442 Size: 416 53 83 399 24 2078 958 1753 Fragments arranged by size: 2078 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2232 2317 2597 4424 1239 Size: 866 127 85 280 1827 2579 Fragments arranged by size: 2579 1827 866 280 127 85 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3889 4754 5052 3889 Size: 865 298 4601 Fragments arranged by size: 4601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4427 882 Size: 617 2928 2219 Fragments arranged by size: 2928 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2412 3555 493 Size: 711 1208 1143 2702 Fragments arranged by size: 2702 1208 1143 711 BsaBI GATnn'nnATC Cuts at: 2037 5758 2037 Size: 3721 2043 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2955 3571 3953 4588 Size: 53 83 186 936 1422 616 382 635 Cuts at: 4588 275 Size: 1451 Fragments arranged by size: 1451 1422 936 635 616 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2510 2540 1178 Size: 30 165 30 1107 30 4402 Fragments arranged by size: 4402 1107 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5764 BseYI C'CCAG_C Cuts at: 2887 4967 5463 2887 Size: 2080 496 3188 Fragments arranged by size: 3188 2080 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2576 1163 Size: 573 840 4351 Fragments arranged by size: 4351 840 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2342 3214 3975 5003 1540 Size: 275 186 341 872 761 1028 2301 Fragments arranged by size: 2301 1028 872 761 341 275 186 BsiHKAI G_wGCw'C Cuts at: 2333 2379 2946 3825 3910 4981 2333 Size: 46 567 879 85 1071 3116 Fragments arranged by size: 3116 1071 879 567 85 46 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2379 2424 2946 3083 3825 3910 Size: 399 543 46 45 522 137 742 85 Cuts at: 3910 4981 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 543 522 399 137 85 46 45 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2243 2298 2435 3119 844 Size: 1399 55 137 684 3489 Fragments arranged by size: 3489 1399 684 137 55 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2477 4259 4433 65 Size: 1183 1229 1782 174 1396 Fragments arranged by size: 1782 1396 1229 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2243 3824 4836 2243 Size: 1581 1012 3171 Fragments arranged by size: 3171 1581 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstXI CCAn_nnnn'nTGG Cuts at: 2104 2536 2779 2104 Size: 432 243 5089 Fragments arranged by size: 5089 432 243 BstYI r'GATC_y Cuts at: 1972 2451 3094 3461 3846 3863 5315 5401 5413 Size: 479 643 367 385 17 1452 86 12 Cuts at: 5413 5759 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 643 479 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2043 513 Size: 552 939 39 4234 Fragments arranged by size: 4234 939 552 39 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 3280 4074 4094 3280 Size: 794 20 4950 Fragments arranged by size: 4950 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2763 2798 529 Size: 35 2199 35 3495 Fragments arranged by size: 3495 2199 35 35 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DraIII CAC_nnn'GTG Cuts at: 2248 2269 2248 Size: 21 5743 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4771 809 Size: 507 3455 1802 Fragments arranged by size: 3455 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2339 3211 4100 Size: 54 1425 50 275 18 509 872 889 Cuts at: 4100 5729 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 872 509 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2339 3211 1537 Size: 275 527 872 4090 Fragments arranged by size: 4090 872 527 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2619 2985 3018 3024 3694 1353 Size: 1266 366 33 6 670 3423 Fragments arranged by size: 3423 1266 670 366 33 6 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2232 2317 2597 3827 4424 5211 Size: 393 473 127 85 280 1230 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 1230 787 597 473 393 280 268 127 85 EcoNI CCTnn'n_nnAGG Cuts at: 2027 2027 Size: 5764 EcoO109I rG'GnC_Cy Cuts at: 2734 2734 Size: 5764 EcoRI G'AATT_C Cuts at: 2999 3073 3451 2999 Size: 74 378 5312 Fragments arranged by size: 5312 378 74 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2740 2740 Size: 5764 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 4911 1678 Size: 3233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2000 2032 2242 2274 3017 Size: 32 135 32 428 32 210 32 743 Cuts at: 3017 3049 4405 4437 4479 4511 1373 Size: 32 1356 32 42 32 2626 Fragments arranged by size: 2626 1356 743 428 210 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 2717 2868 748 Size: 1969 151 3644 Fragments arranged by size: 3644 1969 151 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4878 5062 1305 Size: 3573 184 2007 Fragments arranged by size: 3573 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2382 2534 3722 4081 4240 518 Size: 648 1216 152 1188 359 159 2042 Fragments arranged by size: 2042 1216 1188 648 359 159 152 MspA1I CmG'CkG Cuts at: 1606 2065 2226 2344 2575 2692 2761 2887 3180 Size: 459 161 118 231 117 69 126 293 Cuts at: 3180 3857 5005 5250 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 459 293 245 231 161 126 118 117 69 NcoI C'CATG_G Cuts at: 513 1065 2004 2043 513 Size: 552 939 39 4234 Fragments arranged by size: 4234 939 552 39 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 2803 1108 Size: 1695 4069 PfoI T'CCnGG_A Cuts at: 2028 2661 2028 Size: 633 5131 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PmlI CAC'GTG Cuts at: 2412 2412 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3833 3865 5346 5378 3833 Size: 32 1481 32 4219 Fragments arranged by size: 4219 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 2013 3334 2013 Size: 1321 4443 PstI C_TGCA'G Cuts at: 830 2283 2349 2691 3116 830 Size: 1453 66 342 425 3478 Fragments arranged by size: 3478 1453 425 342 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2065 2575 2761 2887 2065 Size: 510 186 126 4942 Fragments arranged by size: 4942 510 186 126 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SapI GCTCTTCn'nnn_ Cuts at: 2619 2619 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SexAI A'CCwGG_T Cuts at: 2613 3060 2613 Size: 447 5317 SfcI C'TryA_G Cuts at: 826 1045 2279 2345 2687 3112 4247 4928 5119 Size: 219 1234 66 342 425 1135 681 191 Cuts at: 5119 826 Size: 1471 Fragments arranged by size: 1471 1234 1135 681 425 342 219 191 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3868 4769 5031 820 Size: 197 926 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 2462 1517 Size: 945 4819 StyI C'CwwG_G Cuts at: 513 1065 2004 2043 2598 2874 3203 513 Size: 552 939 39 555 276 329 3074 Fragments arranged by size: 3074 939 555 552 329 276 39 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3791 3808 3961 4146 4591 325 Size: 1255 2211 17 153 185 445 1498 Fragments arranged by size: 2211 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2536 2568 5724 5756 2536 Size: 32 3156 32 2544 Fragments arranged by size: 3156 2544 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2050 2779 2800 2050 Size: 729 21 5014 Fragments arranged by size: 5014 729 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI