(Circular) (Six-base) MAPSORT of: pFN21AE1994.seq Check: 4822 from: 1 to: 6427 pFN21AE1994 6427 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4237 278 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2160 3770 1937 Size: 223 1610 4594 Fragments arranged by size: 4594 1610 223 Acc65I G'GTAC_C Cuts at: 3748 3748 Size: 6427 AclI AA'CG_TT Cuts at: 1563 4554 4927 1563 Size: 2991 373 3063 Fragments arranged by size: 3063 2991 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3281 4490 5874 6142 1632 Size: 1649 1209 1384 268 1917 Fragments arranged by size: 1917 1649 1384 1209 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6230 AflIII A'CryG_T Cuts at: 1203 2649 5326 6303 1203 Size: 1446 2677 977 1327 Fragments arranged by size: 2677 1446 1327 977 AgeI A'CCGG_T Cuts at: 5258 5258 Size: 6427 AhdI GACnn_n'nnGTC Cuts at: 5156 5156 Size: 6427 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6427 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2758 1184 Size: 1574 4853 AlwNI CAG_nnn'CTG Cuts at: 2827 3406 3647 4170 5742 2827 Size: 579 241 523 1572 3512 Fragments arranged by size: 3512 1572 579 523 241 ApaI G_GGCC'C Cuts at: 3087 3087 Size: 6427 ApaLI G'TGCA_C Cuts at: 4484 5640 4484 Size: 1156 5271 ApoI r'AATT_y Cuts at: 1493 2090 3736 3962 4114 6223 1493 Size: 597 1646 226 152 2109 1697 Fragments arranged by size: 2109 1697 1646 597 226 152 AseI AT'TA_AT Cuts at: 160 1032 4981 160 Size: 872 3949 1606 Fragments arranged by size: 3949 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 AvaI C'yCGr_G Cuts at: 1870 1943 3752 1870 Size: 73 1809 4545 Fragments arranged by size: 4545 1809 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3458 3491 3458 Size: 33 6394 BamHI G'GATC_C Cuts at: 2036 3757 4124 2036 Size: 1721 367 4339 Fragments arranged by size: 4339 1721 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2144 2600 3748 5203 618 Size: 325 217 627 357 456 1148 1455 1842 Fragments arranged by size: 1842 1455 1148 627 456 357 325 217 BanII G_rGCy'C Cuts at: 1391 2277 2511 2950 3087 3641 3746 1391 Size: 886 234 439 137 554 105 4072 Fragments arranged by size: 4072 886 554 439 234 137 105 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2481 2669 928 Size: 971 582 188 4686 Fragments arranged by size: 4686 971 582 188 BbvCI CC'TCA_GC Cuts at: 2870 2870 Size: 6427 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4618 4652 1903 Size: 34 2681 34 3678 Fragments arranged by size: 3678 2681 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4319 5529 4319 Size: 1210 5217 BclI T'GATC_A Cuts at: 1548 1548 Size: 6427 BfrBI ATG'CAT Cuts at: 3222 3222 Size: 6427 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3143 5038 136 Size: 107 122 71 631 605 1471 1895 1525 Fragments arranged by size: 1895 1525 1471 631 605 122 107 71 BglII A'GATC_T Cuts at: 6422 6422 Size: 6427 BlpI GC'TnA_GC Cuts at: 3844 3844 Size: 6427 Bme1580I G_kGCm'C Cuts at: 1790 2147 2644 3087 4488 5644 1790 Size: 357 497 443 1401 1156 2573 Fragments arranged by size: 2573 1401 1156 497 443 357 BmgBI CAC'GTC Cuts at: 1318 2124 2848 1318 Size: 806 724 4897 Fragments arranged by size: 4897 806 724 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2206 3261 4158 Size: 416 53 83 399 24 789 1055 897 Cuts at: 4158 5116 442 Size: 958 1753 Fragments arranged by size: 1753 1055 958 897 789 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6427 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2083 3373 2083 Size: 1290 5137 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2063 2495 2993 3623 3683 5087 1239 Size: 824 432 498 630 60 1404 2579 Fragments arranged by size: 2579 1404 824 630 498 432 60 Bpu10I CC'TnA_GC Cuts at: 2750 2870 2750 Size: 120 6307 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2351 3165 4552 5417 5715 2351 Size: 814 1387 865 298 3063 Fragments arranged by size: 3063 1387 865 814 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3644 5090 882 Size: 617 2145 1446 2219 Fragments arranged by size: 2219 2145 1446 617 BsaAI yAC'GTr Cuts at: 493 1204 2086 4218 493 Size: 711 882 2132 2702 Fragments arranged by size: 2702 2132 882 711 BsaBI GATnn'nnATC Cuts at: 6421 6421 Size: 6427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4234 4616 5251 275 Size: 53 83 186 936 2701 382 635 1451 Fragments arranged by size: 2701 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2676 2699 3190 4860 5258 5532 5679 6090 2676 Size: 23 491 1670 398 274 147 411 3013 Fragments arranged by size: 3013 1670 491 411 398 274 147 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2681 2711 1178 Size: 30 165 30 1278 30 4894 Fragments arranged by size: 4894 1278 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3488 3493 1186 Size: 2302 5 4120 Fragments arranged by size: 4120 2302 5 BseYI C'CCAG_C Cuts at: 2879 2892 3640 5630 6126 2879 Size: 13 748 1990 496 3180 Fragments arranged by size: 3180 1990 748 496 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2466 2763 3734 1163 Size: 573 730 297 971 3856 Fragments arranged by size: 3856 971 730 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3877 4638 5666 1540 Size: 275 186 1876 761 1028 2301 Fragments arranged by size: 2301 1876 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2277 2511 2847 2950 4488 4573 5644 2277 Size: 234 336 103 1538 85 1071 3060 Fragments arranged by size: 3060 1538 1071 336 234 103 85 BsiWI C'GTAC_G Cuts at: 2181 2181 Size: 6427 BsmI GAATG_Cn' Cuts at: 3938 4031 3938 Size: 93 6334 BsmBI CGTCTCn'nnnn_ Cuts at: 2249 2249 Size: 6427 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2277 2511 2644 2847 2950 3087 Size: 399 357 130 234 133 203 103 137 Cuts at: 3087 3641 3746 4488 4573 5644 1391 Size: 554 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 357 234 203 137 133 130 105 103 85 BspEI T'CCGG_A Cuts at: 2699 3190 6090 2699 Size: 491 2900 3036 Fragments arranged by size: 3036 2900 491 BspHI T'CATG_A Cuts at: 2607 4316 6046 2607 Size: 1709 1730 2988 Fragments arranged by size: 2988 1730 1709 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3782 844 Size: 2938 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4314 1394 Size: 429 2491 3507 Fragments arranged by size: 3507 2491 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3392 4922 5096 65 Size: 1183 2144 1530 174 1396 Fragments arranged by size: 2144 1530 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2313 2460 5071 5258 1700 Size: 110 503 147 2611 187 2869 Fragments arranged by size: 2869 2611 503 187 147 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6427 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6427 BssSI C'ACGA_G Cuts at: 4487 5499 4487 Size: 1012 5415 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6427 BstBI TT'CG_AA Cuts at: 5231 5231 Size: 6427 BstEII G'GTnAC_C Cuts at: 1184 2290 3106 3352 1184 Size: 1106 816 246 4259 Fragments arranged by size: 4259 1106 816 246 BstXI CCAn_nnnn'nTGG Cuts at: 2887 3591 3679 2887 Size: 704 88 5635 Fragments arranged by size: 5635 704 88 BstYI r'GATC_y Cuts at: 1972 2036 2071 3036 3757 4124 4509 4526 5978 Size: 64 35 965 721 367 385 17 1452 Cuts at: 5978 6064 6076 6422 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 965 721 385 367 346 86 64 35 17 12 Bsu36I CC'TnA_GG Cuts at: 2058 3075 3102 3278 2058 Size: 1017 27 176 5207 Fragments arranged by size: 5207 1017 176 27 BtgI C'CryG_G Cuts at: 513 1065 2718 513 Size: 552 1653 4222 Fragments arranged by size: 4222 1653 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2466 487 Size: 683 56 1240 4448 Fragments arranged by size: 4448 1240 683 56 BtsI GCAGTG_nn' Cuts at: 2666 3722 3943 4737 4757 2666 Size: 1056 221 794 20 4336 Fragments arranged by size: 4336 1056 794 221 20 ClaI AT'CG_AT Cuts at: 1554 2405 1554 Size: 851 5576 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6392 DraI TTT'AAA Cuts at: 3731 3731 Size: 6427 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5434 809 Size: 507 4118 1802 Fragments arranged by size: 4118 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2455 3874 4763 Size: 54 1425 50 275 18 625 1419 889 Cuts at: 4763 6392 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1419 889 625 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3874 1537 Size: 275 2062 4090 Fragments arranged by size: 4090 2062 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4357 1353 Size: 3004 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5014 5388 5534 387 Size: 1064 138 3425 374 146 1280 Fragments arranged by size: 3425 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2275 2509 2948 2275 Size: 234 439 5754 Fragments arranged by size: 5754 439 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2063 2495 2993 3281 3623 3683 4490 Size: 393 431 432 498 288 342 60 807 Cuts at: 4490 5087 5874 6142 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 807 787 597 498 432 431 393 342 288 268 60 EcoO109I rG'GnC_Cy Cuts at: 2062 3084 3595 2062 Size: 1022 511 4894 Fragments arranged by size: 4894 1022 511 EcoRI G'AATT_C Cuts at: 3736 4114 3736 Size: 378 6049 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3311 3311 Size: 6427 FspI TGC'GCA Cuts at: 4933 4933 Size: 6427 HaeII r_GCGC'y Cuts at: 1678 5574 1678 Size: 3896 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2407 2439 3413 3445 5068 Size: 32 135 32 835 32 974 32 1623 Cuts at: 5068 5100 5142 5174 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1623 974 835 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2518 3771 4017 669 Size: 988 281 580 1253 246 3079 Fragments arranged by size: 3079 1253 988 580 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6427 HpaI GTT'AAC Cuts at: 4017 4017 Size: 6427 KpnI G_GTAC'C Cuts at: 3752 3752 Size: 6427 MfeI C'AATT_G Cuts at: 4026 4026 Size: 6427 MluI A'CGCG_T Cuts at: 6303 6303 Size: 6427 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3042 5541 5725 1305 Size: 1737 2499 184 2007 Fragments arranged by size: 2499 2007 1737 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2457 10 Size: 54 1425 343 625 3980 Fragments arranged by size: 3980 1425 625 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2327 2606 4385 4744 4903 518 Size: 648 1161 279 1779 359 159 2042 Fragments arranged by size: 2042 1779 1161 648 359 279 159 MspA1I CmG'CkG Cuts at: 1606 2133 2221 2297 2827 2874 2956 3198 3314 Size: 527 88 76 530 47 82 242 116 Cuts at: 3314 3843 4520 5668 5913 1606 Size: 529 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 530 529 527 245 242 116 88 82 76 47 NcoI C'CATG_G Cuts at: 513 1065 2718 513 Size: 552 1653 4222 Fragments arranged by size: 4222 1653 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6427 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6427 NotI GC'GGCC_GC Cuts at: 3874 3874 Size: 6427 NsiI A_TGCA'T Cuts at: 3224 3224 Size: 6427 NspI r_CATG'y Cuts at: 1132 1229 2653 3328 3785 5330 1132 Size: 97 1424 675 457 1545 2229 Fragments arranged by size: 2229 1545 1424 675 457 97 PasI CC'CwG_GG Cuts at: 1348 2931 3406 3591 1348 Size: 1583 475 185 4184 Fragments arranged by size: 4184 1583 475 185 PciI A'CATG_T Cuts at: 2649 5326 2649 Size: 2677 3750 PflMI CCAn_nnn'nTGG Cuts at: 1108 3314 3406 1108 Size: 2206 92 4129 Fragments arranged by size: 4129 2206 92 PfoI T'CCnGG_A Cuts at: 2838 2838 Size: 6427 PmeI GTTT'AAAC Cuts at: 3731 3731 Size: 6427 PmlI CAC'GTG Cuts at: 2086 2086 Size: 6427 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4496 4528 6009 6041 4496 Size: 32 1481 32 4882 Fragments arranged by size: 4882 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2062 2062 Size: 6427 PshAI GACnn'nnGTC Cuts at: 1613 3579 1613 Size: 1966 4461 PsiI TTA'TAA Cuts at: 3997 3997 Size: 6427 PspOMI G'GGCC_C Cuts at: 3083 3083 Size: 6427 PstI C_TGCA'G Cuts at: 830 2355 2944 3348 3779 830 Size: 1525 589 404 431 3478 Fragments arranged by size: 3478 1525 589 431 404 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6427 PvuII CAG'CTG Cuts at: 3198 3314 3198 Size: 116 6311 SacI G_AGCT'C Cuts at: 2277 2511 2950 2277 Size: 234 439 5754 Fragments arranged by size: 5754 439 234 SalI G'TCGA_C Cuts at: 1936 3769 1936 Size: 1833 4594 SbfI CC_TGCA'GG Cuts at: 2944 3779 2944 Size: 835 5592 ScaI AGT'ACT Cuts at: 2739 2906 4675 6387 2739 Size: 167 1769 1712 2779 Fragments arranged by size: 2779 1769 1712 167 SfcI C'TryA_G Cuts at: 826 1045 2161 2351 2755 2940 3344 3775 4910 Size: 219 1116 190 404 185 404 431 1135 Cuts at: 4910 5591 5782 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1116 681 431 404 404 219 191 190 185 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6427 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6427 SmaI CCC'GGG Cuts at: 1872 3754 1872 Size: 1882 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2366 3180 4531 5432 5694 820 Size: 197 926 423 814 1351 901 262 1553 Fragments arranged by size: 1553 1351 926 901 814 423 262 197 SnaBI TAC'GTA Cuts at: 493 4218 493 Size: 3725 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6427 SphI G_CATG'C Cuts at: 1132 3785 1132 Size: 2653 3774 SspI AAT'ATT Cuts at: 5 52 4351 5 Size: 47 4299 2081 Fragments arranged by size: 4299 2081 47 StuI AGG'CCT Cuts at: 1517 3283 3699 1517 Size: 1766 416 4245 Fragments arranged by size: 4245 1766 416 StyI C'CwwG_G Cuts at: 513 1065 2539 2596 2631 2718 3360 3577 3621 Size: 552 1474 57 35 87 642 217 44 Cuts at: 3621 3866 513 Size: 245 3074 Fragments arranged by size: 3074 1474 642 552 245 217 87 57 44 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2188 4454 4471 4624 4809 5254 325 Size: 1255 608 2266 17 153 185 445 1498 Fragments arranged by size: 2266 1498 1255 608 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2737 2904 4673 Size: 274 80 33 51 1443 760 167 1769 Cuts at: 4673 6385 96 Size: 1712 138 Fragments arranged by size: 1769 1712 1443 760 274 167 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3515 3547 6387 6419 3515 Size: 32 2840 32 3523 Fragments arranged by size: 3523 2840 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2156 1316 Size: 840 5587 XbaI T'CTAG_A Cuts at: 3763 3763 Size: 6427 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2029 2537 2679 2029 Size: 508 142 5777 Fragments arranged by size: 5777 508 142 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6427 XmaI C'CCGG_G Cuts at: 1870 3752 1870 Size: 1882 4545 XmnI GAAnn'nnTTC Cuts at: 4556 4556 Size: 6427 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4235 276 Size: 53 83 186 936 2701 2468 Fragments arranged by size: 2701 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI