(Linear) (Six-base) MAPSORT of: KIEE1994.seq Check: 6953 from: 1 to: 1722 KIEE1994.orf With 182 enzymes: SgfI * July 15, 2010 18:52 .. AccI GT'mk_AC Cuts at: 0 155 1722 Size: 155 1567 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1276 1722 Size: 1276 446 AflIII A'CryG_T Cuts at: 0 644 1722 Size: 644 1078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 753 1722 Size: 753 969 AlwNI CAG_nnn'CTG Cuts at: 0 822 1401 1642 1722 Size: 822 579 241 80 Fragments arranged by size: 822 579 241 80 ApaI G_GGCC'C Cuts at: 0 1082 1722 Size: 1082 640 ApoI r'AATT_y Cuts at: 0 85 1722 Size: 85 1637 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1453 1486 1722 Size: 1453 33 236 Fragments arranged by size: 1453 236 33 BamHI G'GATC_C Cuts at: 0 31 1722 Size: 31 1691 BanI G'GyrC_C Cuts at: 0 139 595 1722 Size: 139 456 1127 Fragments arranged by size: 1127 456 139 BanII G_rGCy'C Cuts at: 0 272 506 945 1082 1636 1722 Size: 272 234 439 137 554 86 Fragments arranged by size: 554 439 272 234 137 86 BbsI GAAGACnn'nnnn_ Cuts at: 0 476 664 1722 Size: 476 188 1058 Fragments arranged by size: 1058 476 188 BbvCI CC'TCA_GC Cuts at: 0 865 1722 Size: 865 857 BfrBI ATG'CAT Cuts at: 0 1217 1722 Size: 1217 505 BglI GCCn_nnn'nGGC Cuts at: 0 1138 1722 Size: 1138 584 Bme1580I G_kGCm'C Cuts at: 0 142 639 1082 1722 Size: 142 497 443 640 Fragments arranged by size: 640 497 443 142 BmgBI CAC'GTC Cuts at: 0 119 843 1722 Size: 119 724 879 Fragments arranged by size: 879 724 119 BmrI ACTGGGnnnn_n' Cuts at: 0 201 1256 1722 Size: 201 1055 466 Fragments arranged by size: 1055 466 201 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 78 1368 1722 Size: 78 1290 354 Fragments arranged by size: 1290 354 78 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 490 988 1618 1678 1722 Size: 58 432 498 630 60 44 Fragments arranged by size: 630 498 432 60 58 44 Bpu10I CC'TnA_GC Cuts at: 0 745 865 1722 Size: 745 120 857 Fragments arranged by size: 857 745 120 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 346 1160 1722 Size: 346 814 562 Fragments arranged by size: 814 562 346 BsaI GGTCTCn'nnnn_ Cuts at: 0 1639 1722 Size: 1639 83 BsaAI yAC'GTr Cuts at: 0 81 1722 Size: 81 1641 BsaWI w'CCGG_w Cuts at: 0 671 694 1185 1722 Size: 671 23 491 537 Fragments arranged by size: 671 537 491 23 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 676 706 1722 Size: 676 30 1016 Fragments arranged by size: 1016 676 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1483 1488 1722 Size: 1483 5 234 Fragments arranged by size: 1483 234 5 BseYI C'CCAG_C Cuts at: 0 874 887 1635 1722 Size: 874 13 748 87 Fragments arranged by size: 874 748 87 13 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 461 758 1722 Size: 461 297 964 Fragments arranged by size: 964 461 297 BsiHKAI G_wGCw'C Cuts at: 0 272 506 842 945 1722 Size: 272 234 336 103 777 Fragments arranged by size: 777 336 272 234 103 BsiWI C'GTAC_G Cuts at: 0 176 1722 Size: 176 1546 BsmBI CGTCTCn'nnnn_ Cuts at: 0 244 1722 Size: 244 1478 Bsp1286I G_dGCh'C Cuts at: 0 142 272 506 639 842 945 1082 1636 Size: 142 130 234 133 203 103 137 554 Cuts at: 1636 1722 Size: 86 Fragments arranged by size: 554 234 203 142 137 133 130 103 86 BspEI T'CCGG_A Cuts at: 0 694 1185 1722 Size: 694 491 537 Fragments arranged by size: 694 537 491 BspHI T'CATG_A Cuts at: 0 602 1722 Size: 602 1120 BsrDI GCAATG_nn' Cuts at: 0 1387 1722 Size: 1387 335 BsrFI r'CCGG_y Cuts at: 0 308 455 1722 Size: 308 147 1267 Fragments arranged by size: 1267 308 147 BstEII G'GTnAC_C Cuts at: 0 285 1101 1347 1722 Size: 285 816 246 375 Fragments arranged by size: 816 375 285 246 BstXI CCAn_nnnn'nTGG Cuts at: 0 882 1586 1674 1722 Size: 882 704 88 48 Fragments arranged by size: 882 704 88 48 BstYI r'GATC_y Cuts at: 0 31 66 1031 1722 Size: 31 35 965 691 Fragments arranged by size: 965 691 35 31 Bsu36I CC'TnA_GG Cuts at: 0 53 1070 1097 1273 1722 Size: 53 1017 27 176 449 Fragments arranged by size: 1017 449 176 53 27 BtgI C'CryG_G Cuts at: 0 713 1722 Size: 713 1009 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 461 1722 Size: 461 1261 BtsI GCAGTG_nn' Cuts at: 0 661 1717 1722 Size: 661 1056 5 Fragments arranged by size: 1056 661 5 ClaI AT'CG_AT Cuts at: 0 400 1722 Size: 400 1322 EaeI y'GGCC_r Cuts at: 0 450 1722 Size: 450 1272 EcoICRI GAG'CTC Cuts at: 0 270 504 943 1722 Size: 270 234 439 779 Fragments arranged by size: 779 439 270 234 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 58 490 988 1276 1618 1678 1722 Size: 58 432 498 288 342 60 44 Fragments arranged by size: 498 432 342 288 60 58 44 EcoO109I rG'GnC_Cy Cuts at: 0 57 1079 1590 1722 Size: 57 1022 511 132 Fragments arranged by size: 1022 511 132 57 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1306 1722 Size: 1306 416 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 402 434 1408 1440 1722 Size: 402 32 974 32 282 Fragments arranged by size: 974 402 282 32 32 HincII GTy'rAC Cuts at: 0 513 1722 Size: 513 1209 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1037 1722 Size: 1037 685 MscI TGG'CCA Cuts at: 0 452 1722 Size: 452 1270 MslI CAynn'nnrTG Cuts at: 0 322 601 1722 Size: 322 279 1121 Fragments arranged by size: 1121 322 279 MspA1I CmG'CkG Cuts at: 0 128 216 292 822 869 951 1193 1309 Size: 128 88 76 530 47 82 242 116 Cuts at: 1309 1722 Size: 413 Fragments arranged by size: 530 413 242 128 116 88 82 76 47 NcoI C'CATG_G Cuts at: 0 713 1722 Size: 713 1009 NsiI A_TGCA'T Cuts at: 0 1219 1722 Size: 1219 503 NspI r_CATG'y Cuts at: 0 648 1323 1722 Size: 648 675 399 Fragments arranged by size: 675 648 399 PasI CC'CwG_GG Cuts at: 0 926 1401 1586 1722 Size: 926 475 185 136 Fragments arranged by size: 926 475 185 136 PciI A'CATG_T Cuts at: 0 644 1722 Size: 644 1078 PflMI CCAn_nnn'nTGG Cuts at: 0 1309 1401 1722 Size: 1309 92 321 Fragments arranged by size: 1309 321 92 PfoI T'CCnGG_A Cuts at: 0 833 1722 Size: 833 889 PmlI CAC'GTG Cuts at: 0 81 1722 Size: 81 1641 PpuMI rG'GwC_Cy Cuts at: 0 57 1722 Size: 57 1665 PshAI GACnn'nnGTC Cuts at: 0 1574 1722 Size: 1574 148 PspOMI G'GGCC_C Cuts at: 0 1078 1722 Size: 1078 644 PstI C_TGCA'G Cuts at: 0 350 939 1343 1722 Size: 350 589 404 379 Fragments arranged by size: 589 404 379 350 PvuII CAG'CTG Cuts at: 0 1193 1309 1722 Size: 1193 116 413 Fragments arranged by size: 1193 413 116 SacI G_AGCT'C Cuts at: 0 272 506 945 1722 Size: 272 234 439 777 Fragments arranged by size: 777 439 272 234 SbfI CC_TGCA'GG Cuts at: 0 939 1722 Size: 939 783 ScaI AGT'ACT Cuts at: 0 734 901 1722 Size: 734 167 821 Fragments arranged by size: 821 734 167 SfcI C'TryA_G Cuts at: 0 156 346 750 935 1339 1722 Size: 156 190 404 185 404 383 Fragments arranged by size: 404 404 383 190 185 156 SmlI C'TyrA_G Cuts at: 0 361 1175 1722 Size: 361 814 547 Fragments arranged by size: 814 547 361 StuI AGG'CCT Cuts at: 0 1278 1694 1722 Size: 1278 416 28 Fragments arranged by size: 1278 416 28 StyI C'CwwG_G Cuts at: 0 534 591 626 713 1355 1572 1616 1722 Size: 534 57 35 87 642 217 44 106 Fragments arranged by size: 642 534 217 106 87 57 44 35 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 183 1722 Size: 183 1539 TatI w'GTAC_w Cuts at: 0 732 899 1722 Size: 732 167 823 Fragments arranged by size: 823 732 167 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1510 1542 1722 Size: 1510 32 180 Fragments arranged by size: 1510 180 32 Tth111I GACn'n_nGTC Cuts at: 0 151 1722 Size: 151 1571 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 24 532 674 1722 Size: 24 508 142 1048 Fragments arranged by size: 1048 508 142 24 Enzymes that do cut: AccI AcuI AflIII AlfI AlwNI ApaI ApoI BaeI BamHI BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI EaeI EcoICRI Eco57MI EcoO109I FalI Hin4I HincII MmeI MscI MslI MspA1I NcoI NsiI NspI PasI PciI PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SacI SbfI ScaI SfcI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BbeI BcgI BciVI BclI BglII BlpI BmtI BsaBI BsaHI BsiEI BsmI BspMI BsrBI BsrGI BssHII BssSI BstAPI BstBI BstZ17I CspCI DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI PacI PmeI PpiI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI