Restriction map for
pFN21AE4445
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AhdI AlwNI AocI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BpmI BpuAI
BsaAI BsaBI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrGI BssSI BstBI BstEII
BstPI Bsu36I ClaI DraI DraIII
Eam1105I Eco81I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HindIII KpnI
MaeI MamI MfeI MluI MunI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
SacI SalI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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AccI 1146 GTATAC GT^[AC][GT]AC
AccIII 500 TCCGGA T^CCGGA
AcyI 367 GGCGCC G[GA]^CG[CT]C
Alw44I 870 GTGCAC G^TGCAC
Aor51HI 254 AGCGCT AGC^GCT
ApaI 74 GGGCCC GGGCC^C
ApaLI 870 GTGCAC G^TGCAC
AspHI 870 GTGCAC G[AT]GC[AT]^C
BalI 1189 TGGCCA TGG^CCA
BanII 74 GGGCCC G[GA]GC[CT]^C
BbeI 367 GGCGCC GGCGC^C
BciVI 808 GGATAC GTATCC(6/5)
BglII 1269 AGATCT A^GATCT
BlpI 1068 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1068 GCTGAGC GC^T[ACGT]AGC
BsaHI 367 GGCGCC G[GA]^CG[CT]C
BsaI 1300 GGTCTC GGTCTC(1/5)
BsaWI 500 TCCGGA [AT]^CCGG[AT]
BseAI 500 TCCGGA T^CCGGA
BseRI 337 GAGGAG GAGGAG(10/8)
BsgI 1183 CTGCAC GTGCAG(16/14)
BsiHKAI 870 GTGCAC G[AT]GC[AT]^C
BsmAI 1301 GTCTC GTCTC(1/5)
BspEI 500 TCCGGA T^CCGGA
BsrBI 53 GAGCGG CCGCTC(-3/-3)
BsrDI 1116 GCAATG GCAATG(2/0)
BssHII 319 GCGCGC G^CGCGC
Bst1107I 1146 GTATAC GTA^TAC
BstAPI 1345 GCAGAGTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 449 CCAAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1146 GTATAC GTA^TAC
CelII 1068 GCTGAGC GC^T[ACGT]AGC
CpoI 303 CGGACCG CG^G[AT]CCG
DrdI 739 GACAGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 254 AGCGCT AGC^GCT
EcoNI 351 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1259 GAATTC G^AATTC
HgaI 69 GCGTC GACGC(5/10)
Hin1I 367 GGCGCC G[GA]^CG[CT]C
HincII 640 GTTAAC GT[CT]^[GA]AC
HindII 640 GTTAAC GT[CT]^[GA]AC
HpaI 640 GTTAAC GTT^AAC
KasI 367 GGCGCC G^GCGCC
KspI 362 CCGCGG CCGC^GG
MaeII 455 ACGT A^CGT
MluNI 1189 TGGCCA TGG^CCA
MroI 500 TCCGGA T^CCGGA
MscI 1189 TGGCCA TGG^CCA
MslI 1136 CATCTGTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 367 GGCGCC GG^CGCC
NotI 240 GCGGCCGC GC^GGCCGC
PpuMI 18 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 303 CGGACCG CG^G[AT]CCG
SacII 362 CCGCGG CCGC^GG
SapI 42 GCTCTTC GCTCTTC(1/4)
SfoI 367 GGCGCC GGC^GCC
TaqI 143 TCGA T^CGA
TthHB8I 143 TCGA T^CGA
XcmI 850 CCAGATCACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AfaI 249 GTAC GT^AC
630 GTAC
AlwI 1288 GATCC GGATC(4/5)
1159 GGATC
Asp700I 955 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1042 GAAAGGCTTC
BanI 189 GGTGCC G^G[CT][GA]CC
367 GGCGCC
BcnI 280 CCCGG CC^[GC]GG
333 CCCGG
BsmFI 20 GTCCC GGGAC(10/14)
1339 GTCCC
BsrFI 12 GCCGGC [GA]^CCGG[CT]
132 GCCGGC
BstYI 1269 AGATCT [GA]^GATC[CT]
1287 AGATCC
Cfr10I 12 GCCGGC [GA]^CCGG[CT]
132 GCCGGC
DdeI 540 CTTAG C^T[ACGT]AG
1069 CTGAG
DraII 18 GGGTCCC [GA]G^G[ACGT]CC[CT]
459 GGGGCCT
EcoO109I 18 GGGTCCC [GA]G^G[ACGT]CC[CT]
459 GGGGCCT
EcoT14I 801 CCATGG C^C[AT][AT]GG
1105 CCATGG
HaeII 254 AGCGCT [GA]GCGC^[CT]
367 GGCGCC
HphI 481 GGTGA GGTGA(8/7)
798 TCACC
MflI 1269 AGATCT [GA]^GATC[CT]
1287 AGATCC
MseI 641 TTAA T^TAA
1207 TTAA
MspA1I 127 CCGCTG C[AC]G^C[GT]G
362 CCGCGG
NaeI 12 GCCGGC GCC^GGC
132 GCCGGC
NciI 280 CCCGG CC^[GC]GG
333 CCCGG
NcoI 801 CCATGG C^CATGG
1105 CCATGG
NgoMIV 12 GCCGGC G^CCGGC
132 GCCGGC
PflMI 1100 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAGAAAATGG
RsaI 249 GTAC GT^AC
630 GTAC
SfaNI 396 GCATC GCATC(5/9)
1073 GCATC
StyI 801 CCATGG C^C[AT][AT]GG
1105 CCATGG
Tru9I 641 TTAA T^TAA
1207 TTAA
Tsp45I 797 GTCAC ^GT[GC]AC
908 GTGAC
Van91I 1100 CCAAACCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1192 CCAGAAAATGG
XhoII 1269 AGATCT [GA]^GATC[CT]
1287 AGATCC
XmnI 955 GAACTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1042 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
AvaII BbvI BmyI BsaJI BsiEI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII DsaI EaeI EagI
EarI EclXI Eco52I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI Ksp632I
MaeIII MboI MboII MnlI MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
TfiI TseI Tsp509I TspRI