(Circular) (Six-base) MAPSORT of: pFN21AE4445.seq Check: 1101 from: 1 to: 6088 pFN21AE4445 6088 bp With 182 enzymes: SgfI * March 28, 2012 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3152 3431 1937 Size: 1215 279 4594 Fragments arranged by size: 4594 1215 279 Acc65I G'GTAC_C Cuts at: 3409 3409 Size: 6088 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2275 3299 4151 5535 5803 1632 Size: 643 1024 852 1384 268 1917 Fragments arranged by size: 1917 1384 1024 852 643 268 AfeI AGC'GCT Cuts at: 2261 2261 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 4987 5964 1203 Size: 3784 977 1327 Fragments arranged by size: 3784 1327 977 AgeI A'CCGG_T Cuts at: 4919 4919 Size: 6088 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2337 1184 Size: 1153 4935 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2435 2467 3015 3047 2435 Size: 32 548 32 5476 Fragments arranged by size: 5476 548 32 32 AlwNI CAG_nnn'CTG Cuts at: 3831 5403 3831 Size: 1572 4516 ApaI G_GGCC'C Cuts at: 2083 2083 Size: 6088 ApaLI G'TGCA_C Cuts at: 2875 4145 5301 2875 Size: 1270 1156 3662 Fragments arranged by size: 3662 1270 1156 ApoI r'AATT_y Cuts at: 1493 2719 2834 3264 3397 3623 3775 5884 1493 Size: 1226 115 430 133 226 152 2109 1697 Fragments arranged by size: 2109 1697 1226 430 226 152 133 115 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 3413 1870 Size: 73 1470 4545 Fragments arranged by size: 4545 1470 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2194 2372 3409 4864 618 Size: 325 217 627 407 178 1037 1455 1842 Fragments arranged by size: 1842 1455 1037 627 407 325 217 178 BanII G_rGCy'C Cuts at: 1391 2083 3407 1391 Size: 692 1324 4072 Fragments arranged by size: 4072 1324 692 BbeI G_GCGC'C Cuts at: 2376 2376 Size: 6088 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5117 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4279 4313 1903 Size: 34 2342 34 3678 Fragments arranged by size: 3678 2342 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2807 3980 5190 2807 Size: 1173 1210 3705 Fragments arranged by size: 3705 1210 1173 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4699 136 Size: 107 122 71 631 605 3027 1525 Fragments arranged by size: 3027 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3274 6083 3274 Size: 2809 3279 BlpI GC'TnA_GC Cuts at: 3074 3505 3074 Size: 431 5657 Bme1580I G_kGCm'C Cuts at: 1790 2083 2199 2879 4149 5305 1790 Size: 293 116 680 1270 1156 2573 Fragments arranged by size: 2573 1270 1156 680 293 116 BmgBI CAC'GTC Cuts at: 1318 2461 1318 Size: 1143 4945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2188 3319 3819 Size: 416 53 83 399 24 771 1131 500 Cuts at: 3819 4777 442 Size: 958 1753 Fragments arranged by size: 1753 1131 958 771 500 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4748 1239 Size: 3509 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3311 4751 882 Size: 617 1812 1440 2219 Fragments arranged by size: 2219 1812 1440 617 BsaAI yAC'GTr Cuts at: 493 1204 3879 493 Size: 711 2675 2702 Fragments arranged by size: 2702 2675 711 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 3895 4277 4912 Size: 53 83 186 936 840 1522 382 635 Cuts at: 4912 275 Size: 1451 Fragments arranged by size: 1522 1451 936 840 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2505 4521 4919 5193 5340 5751 2505 Size: 2016 398 274 147 411 2842 Fragments arranged by size: 2842 2016 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2435 2465 2696 2726 3372 Size: 30 165 30 1032 30 231 30 646 Cuts at: 3372 3402 1178 Size: 30 3864 Fragments arranged by size: 3864 1032 646 231 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2357 1186 Size: 1171 4917 BseYI C'CCAG_C Cuts at: 2082 3259 5291 5787 2082 Size: 1177 2032 496 2383 Fragments arranged by size: 2383 2032 1177 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3173 1163 Size: 573 1437 4078 Fragments arranged by size: 4078 1437 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2018 2064 2132 2249 3538 4299 Size: 275 186 17 46 68 117 1289 761 Cuts at: 4299 5327 1540 Size: 1028 2301 Fragments arranged by size: 2301 1289 1028 761 275 186 117 68 46 17 BsiHKAI G_wGCw'C Cuts at: 2879 4149 4234 5305 2879 Size: 1270 85 1071 3662 Fragments arranged by size: 3662 1270 1071 85 BsmI GAATG_Cn' Cuts at: 3599 3692 3599 Size: 93 5995 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2199 2879 3407 4149 4234 5305 Size: 399 293 116 680 528 742 85 1071 Cuts at: 5305 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 680 528 399 293 116 85 BspEI T'CCGG_A Cuts at: 2505 5751 2505 Size: 3246 2842 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2060 3975 1394 Size: 429 237 1915 3507 Fragments arranged by size: 3507 1915 429 237 BsrDI GCAATG_nn' Cuts at: 65 1248 3128 4583 4757 65 Size: 1183 1880 1455 174 1396 Fragments arranged by size: 1880 1455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2017 2137 4732 4919 1700 Size: 110 207 120 2595 187 2869 Fragments arranged by size: 2869 2595 207 187 120 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6088 BssHII G'CGCG_C Cuts at: 1925 2324 1925 Size: 399 5689 BssSI C'ACGA_G Cuts at: 4148 5160 4148 Size: 1012 5076 BstAPI GCAn_nnn'nTGC Cuts at: 794 3356 794 Size: 2562 3526 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6088 BstXI CCAn_nnnn'nTGG Cuts at: 2461 2461 Size: 6088 BstYI r'GATC_y Cuts at: 1972 3274 3292 3418 3785 4170 4187 5639 5725 Size: 1302 18 126 367 385 17 1452 86 Cuts at: 5725 5737 6083 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1302 385 367 346 126 86 18 17 12 BstZ17I GTA'TAC Cuts at: 3153 3153 Size: 6088 BtgI C'CryG_G Cuts at: 513 1065 2367 2806 3110 513 Size: 552 1302 439 304 3491 Fragments arranged by size: 3491 1302 552 439 304 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 2319 3604 4398 4418 2319 Size: 1285 794 20 3989 Fragments arranged by size: 3989 1285 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2832 2867 529 Size: 35 2268 35 3750 Fragments arranged by size: 3750 2268 35 35 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2750 5095 809 Size: 507 1434 2345 1802 Fragments arranged by size: 2345 1802 1434 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2015 2061 2111 Size: 54 1425 50 275 18 185 46 50 Cuts at: 2111 2129 2246 2423 3194 3535 4424 6053 8 Size: 18 117 177 771 341 889 1629 43 Fragments arranged by size: 1629 1425 889 771 341 275 185 177 117 54 50 50 46 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2015 2061 2129 2246 3535 1537 Size: 275 203 46 68 117 1289 4090 Fragments arranged by size: 4090 1289 275 203 117 68 46 EarI CTCTTCn'nnn_ Cuts at: 1353 2054 2363 2504 3314 4018 1353 Size: 701 309 141 810 704 3423 Fragments arranged by size: 3423 810 704 701 309 141 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2275 3299 4151 4748 5535 5803 1239 Size: 393 643 1024 852 597 787 268 1524 Fragments arranged by size: 1524 1024 852 787 643 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2360 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2024 2465 2024 Size: 441 5647 EcoRI G'AATT_C Cuts at: 3264 3397 3775 3264 Size: 133 378 5577 Fragments arranged by size: 5577 378 133 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3068 3068 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2263 2376 5235 1678 Size: 585 113 2859 2531 Fragments arranged by size: 2859 2531 585 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2386 2418 4729 4761 4803 Size: 32 135 32 814 32 2311 32 42 Cuts at: 4803 4835 1373 Size: 32 2626 Fragments arranged by size: 2626 2311 814 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2647 3432 3678 669 Size: 988 281 709 785 246 3079 Fragments arranged by size: 3079 988 785 709 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 2647 3678 2647 Size: 1031 5057 KasI G'GCGC_C Cuts at: 2372 2372 Size: 6088 KpnI G_GTAC'C Cuts at: 3413 3413 Size: 6088 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5202 5386 1305 Size: 3897 184 2007 Fragments arranged by size: 3897 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3196 10 Size: 54 1425 343 1364 2902 Fragments arranged by size: 2902 1425 1364 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3145 4046 4405 4564 518 Size: 648 1979 901 359 159 2042 Fragments arranged by size: 2042 1979 901 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2134 2369 3504 4181 5329 5574 1606 Size: 528 235 1135 677 1148 245 2120 Fragments arranged by size: 2120 1148 1135 677 528 245 235 NaeI GCC'GGC Cuts at: 2019 2139 2019 Size: 120 5968 NarI GG'CG_CC Cuts at: 2373 2373 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 2806 3110 513 Size: 552 1741 304 3491 Fragments arranged by size: 3491 1741 552 304 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 2017 2137 2017 Size: 120 5968 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 2246 3535 2246 Size: 1289 4799 NspI r_CATG'y Cuts at: 1132 1229 3446 4991 1132 Size: 97 2217 1545 2229 Fragments arranged by size: 2229 2217 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6088 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 3111 3203 1108 Size: 2003 92 3993 Fragments arranged by size: 3993 2003 92 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2435 2467 4157 4189 5670 5702 2435 Size: 32 1690 32 1481 32 2821 Fragments arranged by size: 2821 1690 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2024 2024 Size: 6088 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 2079 2079 Size: 6088 PstI C_TGCA'G Cuts at: 830 3440 830 Size: 2610 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 RsrII CG'GwC_CG Cuts at: 2309 2309 Size: 6088 SacII CC_GC'GG Cuts at: 2370 2370 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SanDI GG'GwC_CC Cuts at: 2024 2024 Size: 6088 SapI GCTCTTCn'nnn_ Cuts at: 2054 2054 Size: 6088 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 3436 4571 5252 5443 826 Size: 219 2391 1135 681 191 1471 Fragments arranged by size: 2391 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2374 2374 Size: 6088 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6088 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6088 StyI C'CwwG_G Cuts at: 513 1065 2806 3110 3527 513 Size: 552 1741 304 417 3074 Fragments arranged by size: 3074 1741 552 417 304 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2793 4115 4132 4285 4470 4915 325 Size: 1255 1213 1322 17 153 185 445 1498 Fragments arranged by size: 1498 1322 1255 1213 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4334 6046 96 Size: 274 80 33 51 1443 2357 1712 138 Fragments arranged by size: 2357 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6088 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2862 2862 Size: 6088 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 2964 3051 4217 2964 Size: 87 1166 4835 Fragments arranged by size: 4835 1166 87 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII SacI SexAI SfiI SrfI SwaI