(Linear) (Six-base) MAPSORT of: KIEE4445.seq Check: 7606 from: 1 to: 1383 KIEE4445.orf With 182 enzymes: SgfI * March 22, 2012 18:03 .. AccI GT'mk_AC Cuts at: 0 1147 1383 Size: 1147 236 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 270 1294 1383 Size: 270 1024 89 Fragments arranged by size: 1024 270 89 AfeI AGC'GCT Cuts at: 0 256 1383 Size: 256 1127 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 332 1383 Size: 332 1051 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 430 462 1010 1042 1383 Size: 430 32 548 32 341 Fragments arranged by size: 548 430 341 32 32 ApaI G_GGCC'C Cuts at: 0 78 1383 Size: 78 1305 ApaLI G'TGCA_C Cuts at: 0 870 1383 Size: 870 513 ApoI r'AATT_y Cuts at: 0 714 829 1259 1383 Size: 714 115 430 124 Fragments arranged by size: 714 430 124 115 BanI G'GyrC_C Cuts at: 0 189 367 1383 Size: 189 178 1016 Fragments arranged by size: 1016 189 178 BanII G_rGCy'C Cuts at: 0 78 1383 Size: 78 1305 BbeI G_GCGC'C Cuts at: 0 371 1383 Size: 371 1012 BciVI GTATCCnnnnn_n' Cuts at: 0 802 1383 Size: 802 581 BglII A'GATC_T Cuts at: 0 1269 1383 Size: 1269 114 BlpI GC'TnA_GC Cuts at: 0 1069 1383 Size: 1069 314 Bme1580I G_kGCm'C Cuts at: 0 78 194 874 1383 Size: 78 116 680 509 Fragments arranged by size: 680 509 116 78 BmgBI CAC'GTC Cuts at: 0 456 1383 Size: 456 927 BmrI ACTGGGnnnn_n' Cuts at: 0 183 1314 1383 Size: 183 1131 69 Fragments arranged by size: 1131 183 69 BsaI GGTCTCn'nnnn_ Cuts at: 0 1306 1383 Size: 1306 77 BsaHI Gr'CG_yC Cuts at: 0 368 1383 Size: 368 1015 BsaWI w'CCGG_w Cuts at: 0 500 1383 Size: 500 883 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 430 460 691 721 1383 Size: 430 30 231 30 662 Fragments arranged by size: 662 430 231 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 352 1383 Size: 352 1031 BseYI C'CCAG_C Cuts at: 0 77 1254 1383 Size: 77 1177 129 Fragments arranged by size: 1177 129 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1168 1383 Size: 1168 215 BsiEI CG_ry'CG Cuts at: 0 13 59 127 244 1383 Size: 13 46 68 117 1139 Fragments arranged by size: 1139 117 68 46 13 BsiHKAI G_wGCw'C Cuts at: 0 874 1383 Size: 874 509 Bsp1286I G_dGCh'C Cuts at: 0 78 194 874 1383 Size: 78 116 680 509 Fragments arranged by size: 680 509 116 78 BspEI T'CCGG_A Cuts at: 0 500 1383 Size: 500 883 BsrBI CCG'CTC Cuts at: 0 55 1383 Size: 55 1328 BsrDI GCAATG_nn' Cuts at: 0 1123 1383 Size: 1123 260 BsrFI r'CCGG_y Cuts at: 0 12 132 1383 Size: 12 120 1251 Fragments arranged by size: 1251 120 12 BssHII G'CGCG_C Cuts at: 0 319 1383 Size: 319 1064 BstAPI GCAn_nnn'nTGC Cuts at: 0 1351 1383 Size: 1351 32 BstXI CCAn_nnnn'nTGG Cuts at: 0 456 1383 Size: 456 927 BstYI r'GATC_y Cuts at: 0 1269 1287 1383 Size: 1269 18 96 Fragments arranged by size: 1269 96 18 BstZ17I GTA'TAC Cuts at: 0 1148 1383 Size: 1148 235 BtgI C'CryG_G Cuts at: 0 362 801 1105 1383 Size: 362 439 304 278 Fragments arranged by size: 439 362 304 278 BtsI GCAGTG_nn' Cuts at: 0 314 1383 Size: 314 1069 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 827 862 1383 Size: 827 35 521 Fragments arranged by size: 827 521 35 DrdI GACnn_nn'nnGTC Cuts at: 0 745 1383 Size: 745 638 EaeI y'GGCC_r Cuts at: 0 10 56 106 124 241 418 1189 1383 Size: 10 46 50 18 117 177 771 194 Fragments arranged by size: 771 194 177 117 50 46 18 10 EagI C'GGCC_G Cuts at: 0 10 56 124 241 1383 Size: 10 46 68 117 1142 Fragments arranged by size: 1142 117 68 46 10 EarI CTCTTCn'nnn_ Cuts at: 0 49 358 499 1309 1383 Size: 49 309 141 810 74 Fragments arranged by size: 810 309 141 74 49 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 270 1294 1383 Size: 270 1024 89 Fragments arranged by size: 1024 270 89 EcoNI CCTnn'n_nnAGG Cuts at: 0 355 1383 Size: 355 1028 EcoO109I rG'GnC_Cy Cuts at: 0 19 460 1383 Size: 19 441 923 Fragments arranged by size: 923 441 19 EcoRI G'AATT_C Cuts at: 0 1259 1383 Size: 1259 124 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1063 1383 Size: 1063 320 HaeII r_GCGC'y Cuts at: 0 258 371 1383 Size: 258 113 1012 Fragments arranged by size: 1012 258 113 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 381 413 1383 Size: 381 32 970 Fragments arranged by size: 970 381 32 HincII GTy'rAC Cuts at: 0 642 1383 Size: 642 741 HpaI GTT'AAC Cuts at: 0 642 1383 Size: 642 741 KasI G'GCGC_C Cuts at: 0 367 1383 Size: 367 1016 MscI TGG'CCA Cuts at: 0 1191 1383 Size: 1191 192 MslI CAynn'nnrTG Cuts at: 0 1140 1383 Size: 1140 243 MspA1I CmG'CkG Cuts at: 0 129 364 1383 Size: 129 235 1019 Fragments arranged by size: 1019 235 129 NaeI GCC'GGC Cuts at: 0 14 134 1383 Size: 14 120 1249 Fragments arranged by size: 1249 120 14 NarI GG'CG_CC Cuts at: 0 368 1383 Size: 368 1015 NcoI C'CATG_G Cuts at: 0 801 1105 1383 Size: 801 304 278 Fragments arranged by size: 801 304 278 NgoMIV G'CCGG_C Cuts at: 0 12 132 1383 Size: 12 120 1251 Fragments arranged by size: 1251 120 12 NotI GC'GGCC_GC Cuts at: 0 241 1383 Size: 241 1142 PflMI CCAn_nnn'nTGG Cuts at: 0 1106 1198 1383 Size: 1106 92 185 Fragments arranged by size: 1106 185 92 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 430 462 1383 Size: 430 32 921 Fragments arranged by size: 921 430 32 PpuMI rG'GwC_Cy Cuts at: 0 19 1383 Size: 19 1364 PspOMI G'GGCC_C Cuts at: 0 74 1383 Size: 74 1309 RsrII CG'GwC_CG Cuts at: 0 304 1383 Size: 304 1079 SacII CC_GC'GG Cuts at: 0 365 1383 Size: 365 1018 SanDI GG'GwC_CC Cuts at: 0 19 1383 Size: 19 1364 SapI GCTCTTCn'nnn_ Cuts at: 0 49 1383 Size: 49 1334 SfoI GGC'GCC Cuts at: 0 369 1383 Size: 369 1014 StyI C'CwwG_G Cuts at: 0 801 1105 1383 Size: 801 304 278 Fragments arranged by size: 801 304 278 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 788 1383 Size: 788 595 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 857 1383 Size: 857 526 XmnI GAAnn'nnTTC Cuts at: 0 959 1046 1383 Size: 959 87 337 Fragments arranged by size: 959 337 87 Enzymes that do cut: AccI AcuI AfeI AlfI AloI ApaI ApaLI ApoI BanI BanII BbeI BciVI BglII BlpI Bme1580I BmgBI BmrI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrDI BsrFI BssHII BstAPI BstXI BstYI BstZ17I BtgI BtsI CspCI DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII HpaI KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI PflMI PpiI PpuMI PspOMI RsrII SacII SanDI SapI SfoI StyI TaqII XcmI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlwNI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI BclI BfrBI BglI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsiWI BsmI BsmBI BspHI BspMI BsrGI BssSI BstBI BstEII Bsu36I BtgZI ClaI DraI DraIII EciI EcoICRI EcoRV FseI FspI FspAI HindIII KpnI MfeI MluI MmeI NdeI NheI NruI NsiI NspI PacI PasI PciI PfoI PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI