Restriction map for
pFN21AE1743
(Circular) (Six-base) MAPSORT of: pFN21AE1743.seq Check: 5492 from: 1 to: 5440
pFN21AE1743 5440 bp
With 182 enzymes: SgfI *
August 16, 2010 14:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3250 278
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2783 1937
Size: 846 4594
Acc65I G'GTAC_C
Cuts at: 2761 2761
Size: 5440
AclI AA'CG_TT
Cuts at: 1563 3567 3940 1563
Size: 2004 373 3063
Fragments arranged by size:
3063 2004 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2609 2695 3503 4887 5155 1632
Size: 977 86 808 1384 268 1917
Fragments arranged by size:
1917 1384 977 808 268 86
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5243
AflIII A'CryG_T
Cuts at: 1203 4339 5316 1203
Size: 3136 977 1327
Fragments arranged by size:
3136 1327 977
AgeI A'CCGG_T
Cuts at: 4271 4271
Size: 5440
AhdI GACnn_n'nnGTC
Cuts at: 4169 4169
Size: 5440
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5440
AlwNI CAG_nnn'CTG
Cuts at: 2500 3183 4755 2500
Size: 683 1572 3185
Fragments arranged by size:
3185 1572 683
ApaI G_GGCC'C
Cuts at: 2173 2229 2265 2173
Size: 56 36 5348
Fragments arranged by size:
5348 56 36
ApaLI G'TGCA_C
Cuts at: 3497 4653 3497
Size: 1156 4284
ApoI r'AATT_y
Cuts at: 1493 2749 2975 3127 5236 1493
Size: 1256 226 152 2109 1697
Fragments arranged by size:
2109 1697 1256 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3994 160
Size: 872 2962 1606
Fragments arranged by size:
2962 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2293 2765 1870
Size: 73 73 277 472 4545
Fragments arranged by size:
4545 472 277 73 73
BamHI G'GATC_C
Cuts at: 2770 3137 2770
Size: 367 5073
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2306 2761 4216 618
Size: 325 217 627 519 455 1455 1842
Fragments arranged by size:
1842 1455 627 519 455 325 217
BanII G_rGCy'C
Cuts at: 1391 2173 2229 2265 2284 2595 2693 2759 1391
Size: 782 56 36 19 311 98 66 4072
Fragments arranged by size:
4072 782 311 98 66 56 36 19
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2122 2701 928
Size: 971 223 579 3667
Fragments arranged by size:
3667 971 579 223
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2442 2476 3631 3665 1903
Size: 34 505 34 1155 34 3678
Fragments arranged by size:
3678 1155 505 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3332 4542 3332
Size: 1210 4230
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5440
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4051 136
Size: 107 122 71 631 605 2379 1525
Fragments arranged by size:
2379 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5435 5435
Size: 5440
BlpI GC'TnA_GC
Cuts at: 2857 2857
Size: 5440
Bme1580I G_kGCm'C
Cuts at: 1790 2173 2229 2265 3501 4657 1790
Size: 383 56 36 1236 1156 2573
Fragments arranged by size:
2573 1236 1156 383 56 36
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5440
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2500 3171 4129
Size: 416 53 83 399 24 1083 671 958
Cuts at: 4129 442
Size: 1753
Fragments arranged by size:
1753 1083 958 671 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5440
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4100 1239
Size: 2861 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2524 3565 4430 4728 2129
Size: 395 1041 865 298 2841
Fragments arranged by size:
2841 1041 865 395 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2202 4103 882
Size: 617 703 1901 2219
Fragments arranged by size:
2219 1901 703 617
BsaAI yAC'GTr
Cuts at: 493 1204 3231 493
Size: 711 2027 2702
Fragments arranged by size:
2702 2027 711
BsaBI GATnn'nnATC
Cuts at: 5434 5434
Size: 5440
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3247 3629 4264 275
Size: 53 83 186 936 1714 382 635 1451
Fragments arranged by size:
1714 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2181 3873 4271 4545 4692 5103 2181
Size: 1692 398 274 147 411 2518
Fragments arranged by size:
2518 1692 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5215
Fragments arranged by size:
5215 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2524 2527 1186
Size: 1338 3 4099
Fragments arranged by size:
4099 1338 3
BseYI C'CCAG_C
Cuts at: 2264 4643 5139 2264
Size: 2379 496 2565
Fragments arranged by size:
2565 2379 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2446 1163
Size: 573 710 4157
Fragments arranged by size:
4157 710 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2452 2890 3651 4679 1540
Size: 275 186 451 438 761 1028 2301
Fragments arranged by size:
2301 1028 761 451 438 275 186
BsiHKAI G_wGCw'C
Cuts at: 2034 2089 3501 3586 4657 2034
Size: 55 1412 85 1071 2817
Fragments arranged by size:
2817 1412 1071 85 55
BsmI GAATG_Cn'
Cuts at: 2951 3044 2951
Size: 93 5347
BsmBI CGTCTCn'nnnn_
Cuts at: 2536 2536
Size: 5440
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2034 2089 2173 2229 2265 2284 2595
Size: 399 244 55 84 56 36 19 311
Cuts at: 2595 2693 2759 3501 3586 4657 1391
Size: 98 66 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 311 244 98 85
84 66 56 55 36 19
BspEI T'CCGG_A
Cuts at: 2181 5103 2181
Size: 2922 2518
BspHI T'CATG_A
Cuts at: 3329 5059 3329
Size: 1730 3710
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2795 844
Size: 1951 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3327 1394
Size: 429 1504 3507
Fragments arranged by size:
3507 1504 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3935 4109 65
Size: 1183 2687 174 1396
Fragments arranged by size:
2687 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4084 4271 1700
Size: 110 2274 187 2869
Fragments arranged by size:
2869 2274 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5440
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5440
BssSI C'ACGA_G
Cuts at: 3500 4512 3500
Size: 1012 4428
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5440
BstBI TT'CG_AA
Cuts at: 4244 4244
Size: 5440
BstEII G'GTnAC_C
Cuts at: 1184 2065 2639 1184
Size: 881 574 3985
Fragments arranged by size:
3985 881 574
BstXI CCAn_nnnn'nTGG
Cuts at: 2053 2053
Size: 5440
BstYI r'GATC_y
Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435
Size: 798 367 385 17 1452 86 12 346
Cuts at: 5435 1972
Size: 1977
Fragments arranged by size:
1977 1452 798 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2284 513
Size: 552 939 280 3669
Fragments arranged by size:
3669 939 552 280
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4701
Fragments arranged by size:
4701 683 56
BtsI GCAGTG_nn'
Cuts at: 2956 3750 3770 2956
Size: 794 20 4626
Fragments arranged by size:
4626 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5440
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5405
DraI TTT'AAA
Cuts at: 2744 2744
Size: 5440
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4447 809
Size: 507 3131 1802
Fragments arranged by size:
3131 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2135 2449
Size: 54 1425 50 275 18 213 92 314
Cuts at: 2449 2887 3776 5405 8
Size: 438 889 1629 43
Fragments arranged by size:
1629 1425 889 438 314 275 213 92
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2449 2887 1537
Size: 275 637 438 4090
Fragments arranged by size:
4090 637 438 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2600 3370 1353
Size: 1247 770 3423
Fragments arranged by size:
3423 1247 770
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2347 2383 4027 4401 4547 387
Size: 1064 138 758 36 1644 374 146 1280
Fragments arranged by size:
1644 1280 1064 758 374 146 138 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2609 2695 3503 4100 4887 5155 1239
Size: 393 977 86 808 597 787 268 1524
Fragments arranged by size:
1524 977 808 787 597 393 268 86
EcoNI CCTnn'n_nnAGG
Cuts at: 2539 2539
Size: 5440
EcoO109I rG'GnC_Cy
Cuts at: 2012 2169 2170 2226 2261 2012
Size: 157 1 56 35 5191
Fragments arranged by size:
5191 157 56 35 1
EcoRI G'AATT_C
Cuts at: 2749 3127 2749
Size: 378 5062
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2611 2611
Size: 5440
FspI TGC'GCA
Cuts at: 3946 3946
Size: 5440
HaeII r_GCGC'y
Cuts at: 1678 4587 1678
Size: 2909 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4081 4113 4155 4187 1373
Size: 32 135 32 2509 32 42 32 2626
Fragments arranged by size:
2626 2509 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2784 3030 669
Size: 988 281 846 246 3079
Fragments arranged by size:
3079 988 846 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5440
HpaI GTT'AAC
Cuts at: 3030 3030
Size: 5440
KpnI G_GTAC'C
Cuts at: 2765 2765
Size: 5440
MfeI C'AATT_G
Cuts at: 3039 3039
Size: 5440
MluI A'CGCG_T
Cuts at: 5316 5316
Size: 5440
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2578 4554 4738 1305
Size: 1273 1976 184 2007
Fragments arranged by size:
2007 1976 1273 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 10
Size: 54 1425 343 213 3405
Fragments arranged by size:
3405 1425 343 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3398 3757 3916 518
Size: 648 2232 359 159 2042
Fragments arranged by size:
2232 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2268 2629 2715 2856 3533 4681 4926 1606
Size: 662 361 86 141 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 662 361 245 141 86
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3949
Fragments arranged by size:
3949 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5440
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5440
NotI GC'GGCC_GC
Cuts at: 2887 2887
Size: 5440
NspI r_CATG'y
Cuts at: 1132 1229 2798 4343 1132
Size: 97 1569 1545 2229
Fragments arranged by size:
2229 1569 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2258 2563 1348
Size: 910 305 4225
Fragments arranged by size:
4225 910 305
PciI A'CATG_T
Cuts at: 4339 4339
Size: 5440
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5440
PmeI GTTT'AAAC
Cuts at: 2744 2744
Size: 5440
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 5022 5054 3509
Size: 32 1481 32 3895
Fragments arranged by size:
3895 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2012 2012
Size: 5440
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5440
PsiI TTA'TAA
Cuts at: 3010 3010
Size: 5440
PspOMI G'GGCC_C
Cuts at: 2169 2225 2261 2169
Size: 56 36 5348
Fragments arranged by size:
5348 56 36
PstI C_TGCA'G
Cuts at: 830 2634 2792 830
Size: 1804 158 3478
Fragments arranged by size:
3478 1804 158
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5440
PvuII CAG'CTG
Cuts at: 2629 2629
Size: 5440
SalI G'TCGA_C
Cuts at: 1936 2782 1936
Size: 846 4594
SanDI GG'GwC_CC
Cuts at: 2012 2012
Size: 5440
SapI GCTCTTCn'nnn_
Cuts at: 2600 2600
Size: 5440
SbfI CC_TGCA'GG
Cuts at: 2792 2792
Size: 5440
ScaI AGT'ACT
Cuts at: 3688 5400 3688
Size: 1712 3728
SexAI A'CCwGG_T
Cuts at: 2643 2643
Size: 5440
SfcI C'TryA_G
Cuts at: 826 1045 2630 2788 3923 4604 4795 826
Size: 219 1585 158 1135 681 191 1471
Fragments arranged by size:
1585 1471 1135 681 219 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5440
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5440
SmaI CCC'GGG
Cuts at: 1872 2018 2295 2767 1872
Size: 146 277 472 4545
Fragments arranged by size:
4545 472 277 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2144 2539 3544 4445 4707 820
Size: 197 926 201 395 1005 901 262 1553
Fragments arranged by size:
1553 1005 926 901 395 262 201 197
SnaBI TAC'GTA
Cuts at: 493 3231 493
Size: 2738 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5440
SphI G_CATG'C
Cuts at: 1132 2798 1132
Size: 1666 3774
SspI AAT'ATT
Cuts at: 5 52 3364 5
Size: 47 3312 2081
Fragments arranged by size:
3312 2081 47
StuI AGG'CCT
Cuts at: 1517 2142 2199 1517
Size: 625 57 4758
Fragments arranged by size:
4758 625 57
StyI C'CwwG_G
Cuts at: 513 1065 2004 2194 2310 2879 513
Size: 552 939 190 116 569 3074
Fragments arranged by size:
3074 939 569 552 190 116
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2084 3467 3484 3637 3822 4267 325
Size: 1255 504 1383 17 153 185 445 1498
Fragments arranged by size:
1498 1383 1255 504 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3686 5398 96
Size: 274 80 33 51 1443 1709 1712 138
Fragments arranged by size:
1712 1709 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2013 2045 5400 5432 2013
Size: 32 3355 32 2021
Fragments arranged by size:
3355 2021 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2408 2645 2705 1316
Size: 1092 237 60 4051
Fragments arranged by size:
4051 1092 237 60
XbaI T'CTAG_A
Cuts at: 2776 2776
Size: 5440
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2219 2219
Size: 5440
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5440
XmaI C'CCGG_G
Cuts at: 1870 2016 2293 2765 1870
Size: 146 277 472 4545
Fragments arranged by size:
4545 472 277 146
XmnI GAAnn'nnTTC
Cuts at: 3569 3569
Size: 5440
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3248 276
Size: 53 83 186 936 1714 2468
Fragments arranged by size:
2468 1714 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI
SacII SfiI SfoI SrfI SwaI