################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccI AclI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvrII BamHI BbeI BbvCI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiHKAI BsiWI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MflI MluI MseI MunI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 894 GACGTC GACGT^C AccIII 245 TCCGGA T^CCGGA AcsI 985 AAATTC [GA]^AATT[CT] AcyI 894 GACGTC G[GA]^CG[CT]C AflIII 1043 ACACGT A^C[GA][CT]GT AlwI 147 GATCC GGATC(4/5) ApoI 985 AAATTC [GA]^AATT[CT] AvaI 317 CTCGGG C^[CT]CG[GA]G AvaII 804 GGACC G^G[AT]CC AviII 851 TGCGCA TGC^GCA BbrPI 1044 CACGTG CAC^GTG BcgI 309 GCAAGGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 824 CTAG C^TAG BsaAI 1044 CACGTG [CT]AC^GT[GA] BsaHI 894 GACGTC G[GA]^CG[CT]C BsaWI 245 TCCGGA [AT]^CCGG[AT] BseAI 245 TCCGGA T^CCGGA BsmAI 121 GAGAC GTCTC(1/5) BsmBI 121 GAGACG CGTCTC(1/5) BsmI 613 GAATGC GAATGC(1/-1) BsoBI 317 CTCGGG C^[CT]CG[GA]G BspEI 245 TCCGGA T^CCGGA BspHI 554 TCATGA T^CATGA BsrBI 9 CCGCTC CCGCTC(-3/-3) BstAPI 843 GCAGGACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 147 GATC ^GATC DraIII 625 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 345 CTCTTC CTCTTC(1/4) EcoT14I 899 CCAAGG C^C[AT][AT]GG FspI 851 TGCGCA TGC^GCA HaeII 254 GGCGCT [GA]GCGC^[CT] Hin1I 894 GACGTC G[GA]^CG[CT]C HphI 303 GGTGA GGTGA(8/7) Ksp632I 345 CTCTTC CTCTTC(1/4) MaeI 824 CTAG C^TAG MboI 147 GATC ^GATC MroI 245 TCCGGA T^CCGGA NdeII 147 GATC ^GATC PmaCI 1044 CACGTG CAC^GTG PmlI 1044 CACGTG CAC^GTG PpuMI 803 AGGACCT [GA]G^G[AT]CC[CT] PstI 975 CTGCAG CTGCA^G RcaI 554 TCATGA T^CATGA Sau3AI 147 GATC ^GATC SexAI 806 ACCTGGT A^CC[AT]GGT SfcI 975 CTGCAG C^T[GA][CT]AG SmlI 136 CTCAAG C^T[CT][GA]AG StyI 899 CCAAGG C^C[AT][AT]GG Tsp509I 986 AATT ^AATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### BalI 83 TGGCCA TGG^CCA 218 TGGCCA BanII 548 GAGCCC G[GA]GC[CT]^C 747 GGGCTC BpmI 753 CTCCAG CTGGAG(16/14) 397 CTGGAG BsgI 160 GTGCAG GTGCAG(16/14) 381 GTGCAG BsmFI 313 GGGAC GGGAC(10/14) 5 GTCCC BstXI 722 CCACTGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 755 CCAGGGCACTGG HgaI 306 GACGC GACGC(5/10) 372 GACGC KspI 204 CCGCGG CCGC^GG 936 CCGCGG MluNI 83 TGGCCA TGG^CCA 218 TGGCCA MscI 83 TGGCCA TGG^CCA 218 TGGCCA NaeI 577 GCCGGC GCC^GGC 1013 GCCGGC NgoMIV 577 GCCGGC G^CCGGC 1013 GCCGGC PvuII 214 CAGCTG CAG^CTG 978 CAGCTG SacII 204 CCGCGG CCGC^GG 936 CCGCGG SfaNI 461 GATGC GCATC(5/9) 107 GCATC TaqI 275 TCGA T^CGA 908 TCGA TthHB8I 275 TCGA T^CGA 908 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AccII AciI AfaI AluI BanI BbsI BbvI BglI BmyI BpuAI BsaJI BseRI BsiYI BslI Bsp1286I BspMI BsrFI BsrI BssKI BssSI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MaeIII MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NlaIII NlaIV NspI PleI RsaI Sau96I ScrFI TseI Tsp45I TspRI