(Circular) (Six-base) MAPSORT of: pFN21AE1832.seq Check: 6481 from: 1 to: 5758 pFN21AE1832 5758 bp With 182 enzymes: SgfI * August 16, 2010 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2152 2348 2950 2152 Size: 196 602 4960 Fragments arranged by size: 4960 602 196 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2903 3568 278 Size: 53 83 186 936 1367 665 2468 Fragments arranged by size: 2468 1367 936 665 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2726 3821 5205 5473 1632 Size: 1094 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1095 1094 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5561 AflIII A'CryG_T Cuts at: 1203 3048 4657 5634 1203 Size: 1845 1609 977 1327 Fragments arranged by size: 1845 1609 1327 977 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 2732 1166 Size: 1566 4192 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2882 1184 Size: 1698 4060 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2990 3067 3293 3445 5554 1493 Size: 1497 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 1497 226 152 77 AseI AT'TA_AT Cuts at: 160 1032 4312 160 Size: 872 3280 1606 Fragments arranged by size: 3280 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2322 3083 1870 Size: 73 379 761 4545 Fragments arranged by size: 4545 761 379 73 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2213 2723 2770 3079 4534 Size: 325 217 627 426 510 47 309 1455 Cuts at: 4534 618 Size: 1842 Fragments arranged by size: 1842 1455 627 510 426 325 309 217 47 BanII G_rGCy'C Cuts at: 1391 2557 2756 3077 1391 Size: 1166 199 321 4072 Fragments arranged by size: 4072 1166 321 199 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2038 2047 2287 928 Size: 971 139 9 240 4399 Fragments arranged by size: 4399 971 240 139 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2303 2337 3949 3983 1903 Size: 34 366 34 1612 34 3678 Fragments arranged by size: 3678 1612 366 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2222 2448 2751 Size: 107 122 71 631 605 550 226 303 Cuts at: 2751 4369 136 Size: 1618 1525 Fragments arranged by size: 1618 1525 631 605 550 303 226 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2218 2767 2876 3819 4975 1790 Size: 428 549 109 943 1156 2573 Fragments arranged by size: 2573 1156 943 549 428 109 BmgBI CAC'GTC Cuts at: 1318 2130 2661 1318 Size: 812 531 4415 Fragments arranged by size: 4415 812 531 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2423 2743 4418 1239 Size: 1184 320 1675 2579 Fragments arranged by size: 2579 1675 1184 320 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2126 3883 4748 5046 2126 Size: 1757 865 298 2838 Fragments arranged by size: 2838 1757 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3051 3549 493 Size: 711 1847 498 2702 Fragments arranged by size: 2702 1847 711 498 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2900 3565 3947 4582 Size: 53 83 186 936 1367 665 382 635 Cuts at: 4582 275 Size: 1451 Fragments arranged by size: 1451 1367 936 665 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2250 4191 4589 4863 5010 5421 2250 Size: 1941 398 274 147 411 2587 Fragments arranged by size: 2587 1941 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 2225 2255 1178 Size: 30 165 30 711 30 81 30 4681 Fragments arranged by size: 4681 711 165 81 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2135 2138 2246 2746 1186 Size: 844 105 3 108 500 4198 Fragments arranged by size: 4198 844 500 108 105 3 BseYI C'CCAG_C Cuts at: 2217 2869 4961 5457 2217 Size: 652 2092 496 2518 Fragments arranged by size: 2518 2092 652 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2186 2407 1163 Size: 573 450 221 4514 Fragments arranged by size: 4514 573 450 221 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsmI GAATG_Cn' Cuts at: 2624 3269 3362 2624 Size: 645 93 5020 Fragments arranged by size: 5020 645 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2120 2120 Size: 5758 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2218 2557 2756 2767 2876 3077 3819 Size: 399 428 339 199 11 109 201 742 Cuts at: 3819 3904 4975 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 428 399 339 201 199 109 85 11 BspEI T'CCGG_A Cuts at: 2250 5421 2250 Size: 3171 2587 BspHI T'CATG_A Cuts at: 2559 3647 5377 2559 Size: 1088 1730 2940 Fragments arranged by size: 2940 1730 1088 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2152 2348 2950 3113 844 Size: 1308 196 602 163 3489 Fragments arranged by size: 3489 1308 602 196 163 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3645 1394 Size: 429 193 1629 3507 Fragments arranged by size: 3507 1629 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 4253 4427 65 Size: 1183 3005 174 1396 Fragments arranged by size: 3005 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2256 2582 3018 4402 4589 1700 Size: 110 446 326 436 1384 187 2869 Fragments arranged by size: 2869 1384 446 436 326 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5758 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 2017 2875 3035 3818 4830 2017 Size: 858 160 783 1012 2945 Fragments arranged by size: 2945 1012 858 783 160 BstAPI GCAn_nnn'nTGC Cuts at: 794 2854 794 Size: 2060 3698 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2767 2734 Size: 33 5725 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2209 2629 2941 513 Size: 552 1144 420 312 3330 Fragments arranged by size: 3330 1144 552 420 312 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2464 487 Size: 683 56 1238 3781 Fragments arranged by size: 3781 1238 683 56 BtsI GCAGTG_nn' Cuts at: 2379 2398 3029 3274 4068 4088 2379 Size: 19 631 245 794 20 4049 Fragments arranged by size: 4049 794 631 245 20 19 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DraIII CAC_nnn'GTG Cuts at: 2635 2635 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2088 2223 2938 Size: 54 1425 50 275 18 258 135 715 Cuts at: 2938 3205 4094 5723 8 Size: 267 889 1629 43 Fragments arranged by size: 1629 1425 889 715 275 267 258 135 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2356 3688 1353 Size: 1003 1332 3423 Fragments arranged by size: 3423 1332 1003 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2675 4345 4719 4865 387 Size: 1064 138 1086 1670 374 146 1280 Fragments arranged by size: 1670 1280 1086 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2423 2726 2743 3821 4418 5205 5473 Size: 393 791 303 17 1078 597 787 268 Cuts at: 5473 1239 Size: 1524 Fragments arranged by size: 1524 1078 791 787 597 393 303 268 17 EcoO109I rG'GnC_Cy Cuts at: 2146 2538 2809 2146 Size: 392 271 5095 Fragments arranged by size: 5095 392 271 EcoRI G'AATT_C Cuts at: 3067 3445 3067 Size: 378 5380 FspI TGC'GCA Cuts at: 2858 4264 2858 Size: 1406 4352 HaeII r_GCGC'y Cuts at: 1678 2263 4905 1678 Size: 585 2642 2531 Fragments arranged by size: 2642 2531 585 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373 Size: 32 135 32 2827 32 42 32 2626 Fragments arranged by size: 2827 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4872 5056 1305 Size: 3567 184 2007 Fragments arranged by size: 3567 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2090 2225 10 Size: 54 1425 343 258 135 3543 Fragments arranged by size: 3543 1425 343 258 135 54 MslI CAynn'nnrTG Cuts at: 518 1166 2574 2612 2732 2802 3716 4075 4234 Size: 648 1408 38 120 70 914 359 159 Cuts at: 4234 518 Size: 2042 Fragments arranged by size: 2042 1408 914 648 359 159 120 70 38 MspA1I CmG'CkG Cuts at: 1606 2211 2221 2862 2943 2985 3031 3174 3851 Size: 605 10 641 81 42 46 143 677 Cuts at: 3851 4999 5244 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 641 605 245 143 81 46 42 10 NaeI GCC'GGC Cuts at: 2584 3020 2584 Size: 436 5322 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5206 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NgoMIV G'CCGG_C Cuts at: 2582 3018 2582 Size: 436 5322 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2611 2857 2892 3116 4661 1132 Size: 97 1382 246 35 224 1545 2229 Fragments arranged by size: 2229 1545 1382 246 224 97 35 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 4657 4657 Size: 5758 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PmlI CAC'GTG Cuts at: 3051 3051 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2809 2809 Size: 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 3328 3328 Size: 5758 PstI C_TGCA'G Cuts at: 830 2984 3110 830 Size: 2154 126 3478 Fragments arranged by size: 3478 2154 126 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 PvuII CAG'CTG Cuts at: 2221 2985 2221 Size: 764 4994 SacII CC_GC'GG Cuts at: 2212 2944 2212 Size: 732 5026 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 5758 SfcI C'TryA_G Cuts at: 826 1045 2980 3106 4241 4922 5113 826 Size: 219 1935 126 1135 681 191 1471 Fragments arranged by size: 1935 1471 1135 681 219 191 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 3085 1872 Size: 1213 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 3862 4763 5025 820 Size: 197 926 198 1721 901 262 1553 Fragments arranged by size: 1721 1553 926 901 262 198 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 3682 5 Size: 47 3630 2081 Fragments arranged by size: 3630 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5758 StyI C'CwwG_G Cuts at: 513 1065 2904 3197 513 Size: 552 1839 293 3074 Fragments arranged by size: 3074 1839 552 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 4004 5716 Size: 274 80 33 51 1443 840 1187 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1443 1187 840 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2114 2146 2436 2468 2609 2641 5718 5750 2114 Size: 32 290 32 141 32 3077 32 2122 Fragments arranged by size: 3077 2122 290 141 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 3094 3094 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 3083 1870 Size: 1213 4545 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2901 3566 276 Size: 53 83 186 936 1367 665 2468 Fragments arranged by size: 2468 1367 936 665 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SfoI SrfI SwaI XcmI