(Linear) (Six-base) MAPSORT of: KIEE1832.seq Check: 1558 from: 1 to: 1053 KIEE1832.orf With 182 enzymes: SgfI * July 15, 2010 18:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 147 343 945 1053 Size: 147 196 602 108 Fragments arranged by size: 602 196 147 108 AatII G_ACGT'C Cuts at: 0 898 1053 Size: 898 155 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 721 1053 Size: 721 332 AflIII A'CryG_T Cuts at: 0 1043 1053 Size: 1043 10 AleI CACnn'nnGTG Cuts at: 0 727 1053 Size: 727 326 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 877 1053 Size: 877 176 ApoI r'AATT_y Cuts at: 0 985 1053 Size: 985 68 AvaI C'yCGr_G Cuts at: 0 317 1053 Size: 317 736 BanI G'GyrC_C Cuts at: 0 208 718 765 1053 Size: 208 510 47 288 Fragments arranged by size: 510 288 208 47 BanII G_rGCy'C Cuts at: 0 552 751 1053 Size: 552 199 302 Fragments arranged by size: 552 302 199 BbsI GAAGACnn'nnnn_ Cuts at: 0 33 42 282 1053 Size: 33 9 240 771 Fragments arranged by size: 771 240 33 9 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 298 332 1053 Size: 298 34 721 Fragments arranged by size: 721 298 34 BglI GCCn_nnn'nGGC Cuts at: 0 217 443 746 1053 Size: 217 226 303 307 Fragments arranged by size: 307 303 226 217 Bme1580I G_kGCm'C Cuts at: 0 213 762 871 1053 Size: 213 549 109 182 Fragments arranged by size: 549 213 182 109 BmgBI CAC'GTC Cuts at: 0 125 656 1053 Size: 125 531 397 Fragments arranged by size: 531 397 125 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 738 1053 Size: 418 320 315 Fragments arranged by size: 418 320 315 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 121 1053 Size: 121 932 BsaAI yAC'GTr Cuts at: 0 1046 1053 Size: 1046 7 BsaHI Gr'CG_yC Cuts at: 0 895 1053 Size: 895 158 BsaWI w'CCGG_w Cuts at: 0 245 1053 Size: 245 808 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 109 139 220 250 1053 Size: 109 30 81 30 803 Fragments arranged by size: 803 109 81 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 25 130 133 241 741 1053 Size: 25 105 3 108 500 312 Fragments arranged by size: 500 312 108 105 25 3 BseYI C'CCAG_C Cuts at: 0 212 864 1053 Size: 212 652 189 Fragments arranged by size: 652 212 189 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 181 402 1053 Size: 181 221 651 Fragments arranged by size: 651 221 181 BsmI GAATG_Cn' Cuts at: 0 619 1053 Size: 619 434 BsmBI CGTCTCn'nnnn_ Cuts at: 0 115 1053 Size: 115 938 Bsp1286I G_dGCh'C Cuts at: 0 213 552 751 762 871 1053 Size: 213 339 199 11 109 182 Fragments arranged by size: 339 213 199 182 109 11 BspEI T'CCGG_A Cuts at: 0 245 1053 Size: 245 808 BspHI T'CATG_A Cuts at: 0 554 1053 Size: 554 499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 147 343 945 1053 Size: 147 196 602 108 Fragments arranged by size: 602 196 147 108 BsrBI CCG'CTC Cuts at: 0 11 1053 Size: 11 1042 BsrFI r'CCGG_y Cuts at: 0 251 577 1013 1053 Size: 251 326 436 40 Fragments arranged by size: 436 326 251 40 BssSI C'ACGA_G Cuts at: 0 12 870 1030 1053 Size: 12 858 160 23 Fragments arranged by size: 858 160 23 12 BstAPI GCAn_nnn'nTGC Cuts at: 0 849 1053 Size: 849 204 BstXI CCAn_nnnn'nTGG Cuts at: 0 729 762 1053 Size: 729 33 291 Fragments arranged by size: 729 291 33 BtgI C'CryG_G Cuts at: 0 204 624 936 1053 Size: 204 420 312 117 Fragments arranged by size: 420 312 204 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 459 1053 Size: 459 594 BtsI GCAGTG_nn' Cuts at: 0 374 393 1024 1053 Size: 374 19 631 29 Fragments arranged by size: 631 374 29 19 DraIII CAC_nnn'GTG Cuts at: 0 630 1053 Size: 630 423 EaeI y'GGCC_r Cuts at: 0 83 218 933 1053 Size: 83 135 715 120 Fragments arranged by size: 715 135 120 83 EarI CTCTTCn'nnn_ Cuts at: 0 351 1053 Size: 351 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 670 1053 Size: 670 383 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 418 721 738 1053 Size: 418 303 17 315 Fragments arranged by size: 418 315 303 17 EcoO109I rG'GnC_Cy Cuts at: 0 141 533 804 1053 Size: 141 392 271 249 Fragments arranged by size: 392 271 249 141 FspI TGC'GCA Cuts at: 0 853 1053 Size: 853 200 HaeII r_GCGC'y Cuts at: 0 258 1053 Size: 258 795 MscI TGG'CCA Cuts at: 0 85 220 1053 Size: 85 135 833 Fragments arranged by size: 833 135 85 MslI CAynn'nnrTG Cuts at: 0 569 607 727 797 1053 Size: 569 38 120 70 256 Fragments arranged by size: 569 256 120 70 38 MspA1I CmG'CkG Cuts at: 0 206 216 857 938 980 1026 1053 Size: 206 10 641 81 42 46 27 Fragments arranged by size: 641 206 81 46 42 27 10 NaeI GCC'GGC Cuts at: 0 579 1015 1053 Size: 579 436 38 Fragments arranged by size: 579 436 38 NgoMIV G'CCGG_C Cuts at: 0 577 1013 1053 Size: 577 436 40 Fragments arranged by size: 577 436 40 NspI r_CATG'y Cuts at: 0 606 852 887 1053 Size: 606 246 35 166 Fragments arranged by size: 606 246 166 35 PmlI CAC'GTG Cuts at: 0 1046 1053 Size: 1046 7 PpuMI rG'GwC_Cy Cuts at: 0 804 1053 Size: 804 249 PstI C_TGCA'G Cuts at: 0 979 1053 Size: 979 74 PvuII CAG'CTG Cuts at: 0 216 980 1053 Size: 216 764 73 Fragments arranged by size: 764 216 73 SacII CC_GC'GG Cuts at: 0 207 939 1053 Size: 207 732 114 Fragments arranged by size: 732 207 114 SexAI A'CCwGG_T Cuts at: 0 806 1053 Size: 806 247 SfcI C'TryA_G Cuts at: 0 975 1053 Size: 975 78 SmlI C'TyrA_G Cuts at: 0 136 1053 Size: 136 917 StyI C'CwwG_G Cuts at: 0 899 1053 Size: 899 154 TatI w'GTAC_w Cuts at: 0 812 1053 Size: 812 241 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 109 141 431 463 604 636 1053 Size: 109 32 290 32 141 32 417 Fragments arranged by size: 417 290 141 109 32 32 32 ZraI GAC'GTC Cuts at: 0 896 1053 Size: 896 157 Enzymes that do cut: AarI AatII AcuI AflIII AleI AlfI ApoI AvaI BanI BanII BbsI BcgI BglI Bme1580I BmgBI BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I FspI HaeII MscI MslI MspA1I NaeI NgoMIV NspI PmlI PpuMI PstI PvuII SacII SexAI SfcI SmlI StyI TatI TstI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglII BlpI BmrI BmtI BplI Bpu10I BsaI BsaBI BsiEI BsiHKAI BsiWI BsrDI BsrGI BssHII BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI XmnI