Restriction map for
pFN21AE5343
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# NO CUT ENZYMES ( 139 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BciVI BclI BcnI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaWI BseAI BsgI
BsiWI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HpaI KasI
KpnI Ksp632I KspI MaeI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NarI NciI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I Van91I XbaI XcmI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccII 82 CGCG CG^CG
AcyI 409 GACGCC G[GA]^CG[CT]C
AfaI 261 GTAC GT^AC
AlwI 297 GATCC GGATC(4/5)
AocI 44 CCTCAGG CC^T[ACGT]AGG
ApaI 282 GGGCCC GGGCC^C
AspHI 228 GAGCTC G[AT]GC[AT]^C
AspI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 168 CACGTG CAC^GTG
BbsI 597 GAAGAC GAAGAC(2/6)
BcgI 362 GCAATGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 597 GAAGAC GAAGAC(2/6)
BsaAI 168 CACGTG [CT]AC^GT[GA]
BsaBI 297 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 409 GACGCC G[GA]^CG[CT]C
BsaI 793 GAGACC GGTCTC(1/5)
BsiEI 264 CGACCG CG[GA][CT]^CG
BsiHKAI 228 GAGCTC G[AT]GC[AT]^C
BsrDI 362 GCAATG GCAATG(2/0)
BssSI 345 CTCGTG CACGAG(-5/-1)
BstUI 82 CGCG CG^CG
BstXI 165 CCACACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 44 CCTCAGG CC^T[ACGT]AGG
CfoI 395 GCGC GCG^C
DpnII 297 GATC ^GATC
Eco81I 44 CCTCAGG CC^T[ACGT]AGG
HaeII 394 AGCGCC [GA]GCGC^[CT]
HgaI 409 GACGC GACGC(5/10)
HhaI 395 GCGC GCG^C
Hin1I 409 GACGCC G[GA]^CG[CT]C
HinP1I 395 GCGC G^CGC
HindIII 156 AAGCTT A^AGCTT
MaeII 169 ACGT A^CGT
MamI 297 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 297 GATC ^GATC
MslI 166 CACACGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 82 CGCG CG^CG
NaeI 211 GCCGGC GCC^GGC
NdeII 297 GATC ^GATC
NgoMIV 211 GCCGGC G^CCGGC
PflFI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 168 CACGTG CAC^GTG
PmlI 168 CACGTG CAC^GTG
RsaI 261 GTAC GT^AC
SacI 228 GAGCTC GAGCT^C
Sau3AI 297 GATC ^GATC
SexAI 242 ACCAGGT A^CC[AT]GGT
Tth111I 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 167 ACACGT A^C[GA][CT]GT
169 ACGTGT
BanII 228 GAGCTC G[GA]GC[CT]^C
282 GGGCCC
BmyI 228 GAGCTC G[AGT]GC[ACT]^C
282 GGGCCC
BseRI 25 CTCCTC GAGGAG(10/8)
790 GAGGAG
BsmBI 490 CGTCTC CGTCTC(1/5)
407 GAGACG
Bsp1286I 228 GAGCTC G[AGT]GC[ACT]^C
282 GGGCCC
BsrBI 342 CCGCTC CCGCTC(-3/-3)
627 CCGCTC
BsrFI 211 GCCGGC [GA]^CCGG[CT]
478 ACCGGC
Cfr10I 211 GCCGGC [GA]^CCGG[CT]
478 ACCGGC
DdeI 16 CTCAG C^T[ACGT]AG
45 CTCAG
EaeI 35 TGGCCG [CT]^GGCC[GA]
213 CGGCCA
EcoT14I 602 CCATGG C^C[AT][AT]GG
696 CCATGG
FauI 436 CCCGC CCCGC(4/6)
737 CCCGC
HinfI 646 GACTC G^A[ACGT]TC
764 GAGTC
MboII 594 GAAGA GAAGA(8/7)
597 GAAGA
NcoI 602 CCATGG C^CATGG
696 CCATGG
NlaIII 603 CATG CATG^
697 CATG
PaeR7I 225 CTCGAG C^TCGAG
761 CTCGAG
PleI 646 GACTC GAGTC(4/5)
764 GAGTC
PpuMI 314 GGGACCT [GA]G^G[AT]CC[CT]
654 AGGACCC
PstI 136 CTGCAG CTGCA^G
523 CTGCAG
PvuII 114 CAGCTG CAG^CTG
400 CAGCTG
SmlI 225 CTCGAG C^T[CT][GA]AG
761 CTCGAG
StyI 602 CCATGG C^C[AT][AT]GG
696 CCATGG
TspRI 217 CACTG CA[GC]TG[ACGT][ACGT]^
559 CACTG
XhoI 225 CTCGAG C^TCGAG
761 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AluI AvaI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI BspMI BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DraII
DsaI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeIII MnlI MspA1I MspI MvaI
MwoI NlaIV Sau96I ScrFI SfaNI
SfcI TaqI TseI Tsp45I TthHB8I