(Linear) (Six-base) MAPSORT of: KIEE5343.seq Check: 8007 from: 1 to: 810 KIEE5343.orf With 182 enzymes: SgfI * January 29, 2014 11:56 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 282 810 Size: 282 528 AflIII A'CryG_T Cuts at: 0 167 169 810 Size: 167 2 641 Fragments arranged by size: 641 167 2 AleI CACnn'nnGTG Cuts at: 0 170 810 Size: 170 640 ApaI G_GGCC'C Cuts at: 0 286 810 Size: 286 524 AvaI C'yCGr_G Cuts at: 0 225 286 761 810 Size: 225 61 475 49 Fragments arranged by size: 475 225 61 49 BanII G_rGCy'C Cuts at: 0 232 286 810 Size: 232 54 524 Fragments arranged by size: 524 232 54 BbsI GAAGACnn'nnnn_ Cuts at: 0 604 810 Size: 604 206 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 351 385 810 Size: 351 34 425 Fragments arranged by size: 425 351 34 Bme1580I G_kGCm'C Cuts at: 0 286 810 Size: 286 524 BmrI ACTGGGnnnn_n' Cuts at: 0 207 810 Size: 207 603 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 676 810 Size: 676 134 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 635 671 810 Size: 549 86 36 139 Fragments arranged by size: 549 139 86 36 BsaI GGTCTCn'nnnn_ Cuts at: 0 787 810 Size: 787 23 BsaAI yAC'GTr Cuts at: 0 170 810 Size: 170 640 BsaBI GATnn'nnATC Cuts at: 0 301 810 Size: 301 509 BsaHI Gr'CG_yC Cuts at: 0 410 810 Size: 410 400 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 398 428 613 643 810 Size: 398 30 185 30 167 Fragments arranged by size: 398 185 167 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 16 805 810 Size: 16 789 5 Fragments arranged by size: 789 16 5 BseYI C'CCAG_C Cuts at: 0 398 402 482 810 Size: 398 4 80 328 Fragments arranged by size: 398 328 80 4 BsiEI CG_ry'CG Cuts at: 0 267 810 Size: 267 543 BsiHKAI G_wGCw'C Cuts at: 0 232 810 Size: 232 578 BsmBI CGTCTCn'nnnn_ Cuts at: 0 401 496 810 Size: 401 95 314 Fragments arranged by size: 401 314 95 Bsp1286I G_dGCh'C Cuts at: 0 232 286 810 Size: 232 54 524 Fragments arranged by size: 524 232 54 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 282 681 810 Size: 117 165 399 129 Fragments arranged by size: 399 165 129 117 BsrBI CCG'CTC Cuts at: 0 344 629 810 Size: 344 285 181 Fragments arranged by size: 344 285 181 BsrDI GCAATG_nn' Cuts at: 0 369 810 Size: 369 441 BsrFI r'CCGG_y Cuts at: 0 211 478 810 Size: 211 267 332 Fragments arranged by size: 332 267 211 BssSI C'ACGA_G Cuts at: 0 345 810 Size: 345 465 BstXI CCAn_nnnn'nTGG Cuts at: 0 172 810 Size: 172 638 Bsu36I CC'TnA_GG Cuts at: 0 45 810 Size: 45 765 BtgI C'CryG_G Cuts at: 0 542 602 696 749 810 Size: 542 60 94 53 61 Fragments arranged by size: 542 94 61 60 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 171 354 810 Size: 171 183 456 Fragments arranged by size: 456 183 171 BtsI GCAGTG_nn' Cuts at: 0 216 558 810 Size: 216 342 252 Fragments arranged by size: 342 252 216 EaeI y'GGCC_r Cuts at: 0 35 213 810 Size: 35 178 597 Fragments arranged by size: 597 178 35 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 759 810 Size: 759 51 EcoICRI GAG'CTC Cuts at: 0 230 810 Size: 230 580 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 549 635 671 810 Size: 549 86 36 139 Fragments arranged by size: 549 139 86 36 EcoO109I rG'GnC_Cy Cuts at: 0 282 283 315 531 655 810 Size: 282 1 32 216 124 155 Fragments arranged by size: 282 216 155 124 32 1 HaeII r_GCGC'y Cuts at: 0 398 810 Size: 398 412 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 398 430 608 613 640 645 810 Size: 398 32 178 5 27 5 165 Fragments arranged by size: 398 178 165 32 27 5 5 HindIII A'AGCT_T Cuts at: 0 156 810 Size: 156 654 MslI CAynn'nnrTG Cuts at: 0 170 810 Size: 170 640 MspA1I CmG'CkG Cuts at: 0 116 402 439 810 Size: 116 286 37 371 Fragments arranged by size: 371 286 116 37 NaeI GCC'GGC Cuts at: 0 213 810 Size: 213 597 NcoI C'CATG_G Cuts at: 0 602 696 810 Size: 602 94 114 Fragments arranged by size: 602 114 94 NgoMIV G'CCGG_C Cuts at: 0 211 810 Size: 211 599 PfoI T'CCnGG_A Cuts at: 0 232 810 Size: 232 578 PmlI CAC'GTG Cuts at: 0 170 810 Size: 170 640 PpuMI rG'GwC_Cy Cuts at: 0 315 655 810 Size: 315 340 155 Fragments arranged by size: 340 315 155 PspOMI G'GGCC_C Cuts at: 0 282 810 Size: 282 528 PspXI vC'TCGA_Gb Cuts at: 0 761 810 Size: 761 49 PstI C_TGCA'G Cuts at: 0 140 527 810 Size: 140 387 283 Fragments arranged by size: 387 283 140 PvuII CAG'CTG Cuts at: 0 116 402 810 Size: 116 286 408 Fragments arranged by size: 408 286 116 SacI G_AGCT'C Cuts at: 0 232 810 Size: 232 578 SexAI A'CCwGG_T Cuts at: 0 242 810 Size: 242 568 SfcI C'TryA_G Cuts at: 0 111 136 523 810 Size: 111 25 387 287 Fragments arranged by size: 387 287 111 25 SmlI C'TyrA_G Cuts at: 0 225 761 810 Size: 225 536 49 Fragments arranged by size: 536 225 49 StyI C'CwwG_G Cuts at: 0 602 696 810 Size: 602 94 114 Fragments arranged by size: 602 114 94 Tth111I GACn'n_nGTC Cuts at: 0 412 810 Size: 412 398 XhoI C'TCGA_G Cuts at: 0 225 761 810 Size: 225 536 49 Fragments arranged by size: 536 225 49 Enzymes that do cut: AarI AflIII AleI ApaI AvaI BanII BbsI BcgI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssSI BstXI Bsu36I BtgI BtgZI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HindIII MslI MspA1I NaeI NcoI NgoMIV PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI SexAI SfcI SmlI StyI Tth111I XhoI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI Bpu10I BpuEI BsaWI BsgI BsiWI BsmI BspEI BspHI BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I ClaI CspCI DraI DraIII DrdI EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI XbaI XcmI XmaI XmnI ZraI