Restriction map for
pFN21AE4024
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BbsI BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KpnI KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfuI SgrAI
SmlI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
TfiI Tru9I Tsp509I TspRI Tth111I
TthHB8I Van91I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AcyI 458 GGCGCC G[GA]^CG[CT]C
AocI 358 CCTCAGG CC^T[ACGT]AGG
ApaI 508 GGGCCC GGGCC^C
AspHI 541 GTGCTC G[AT]GC[AT]^C
AvaII 233 GGTCC G^G[AT]CC
BamHI 372 GGATCC G^GATCC
BanI 458 GGCGCC G^G[CT][GA]CC
BbeI 458 GGCGCC GGCGC^C
BbrPI 445 CACGTG CAC^GTG
BsaAI 445 CACGTG [CT]AC^GT[GA]
BsaHI 458 GGCGCC G[GA]^CG[CT]C
BseRI 624 CTCCTC GAGGAG(10/8)
BsiHKAI 541 GTGCTC G[AT]GC[AT]^C
BsmAI 593 GTCTC GTCTC(1/5)
BspMI 241 ACCTGC ACCTGC(4/8)
BsrDI 675 CATTGC GCAATG(2/0)
BstEII 261 GGTCACC G^GT[ACGT]ACC
BstPI 261 GGTCACC G^GT[ACGT]ACC
BstYI 372 GGATCC [GA]^GATC[CT]
Bsu36I 358 CCTCAGG CC^T[ACGT]AGG
DdeI 359 CTCAG C^T[ACGT]AG
DpnII 373 GATC ^GATC
EaeI 464 TGGCCG [CT]^GGCC[GA]
Eco81I 358 CCTCAGG CC^T[ACGT]AGG
EcoNI 623 CCTCCTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 261 GGTCACC G^GT[ACGT]ACC
FauI 246 CCCGC CCCGC(4/6)
HaeII 458 GGCGCC [GA]GCGC^[CT]
Hin1I 458 GGCGCC G[GA]^CG[CT]C
HinfI 633 GACTC G^A[ACGT]TC
KasI 458 GGCGCC G^GCGCC
MaeII 446 ACGT A^CGT
MboI 373 GATC ^GATC
MflI 372 GGATCC [GA]^GATC[CT]
MslI 63 CACCGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 458 GGCGCC GG^CGCC
NdeII 373 GATC ^GATC
NspI 177 GCATGC [GA]CATG^[CT]
PleI 633 GACTC GAGTC(4/5)
PmaCI 445 CACGTG CAC^GTG
PmlI 445 CACGTG CAC^GTG
Sau3AI 373 GATC ^GATC
SexAI 229 ACCTGGT A^CC[AT]GGT
SfoI 458 GGCGCC GGC^GCC
SphI 177 GCATGC GCATG^C
XcmI 59 CCACCACCGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 372 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
AccII 298 CGCG CG^CG
468 CGCG
AlwI 373 GATCC GGATC(4/5)
372 GGATC
AlwNI 361 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
612 CAGCTGCTG
BanII 133 GAGCCC G[GA]GC[CT]^C
508 GGGCCC
BpmI 627 CTCCAG CTGGAG(16/14)
112 CTGGAG
BsgI 6 CTGCAC GTGCAG(16/14)
217 GTGCAG
BstF5I 417 GGATG GGATG(2/0)
426 GGATG
BstUI 298 CGCG CG^CG
468 CGCG
DraII 278 GGGGCCT [GA]G^G[ACGT]CC[CT]
508 GGGCCCT
EcoO109I 278 GGGGCCT [GA]G^G[ACGT]CC[CT]
508 GGGCCCT
FokI 417 GGATG GGATG(9/13)
426 GGATG
MaeIII 107 GTGAC ^GT[ACGT]AC
262 GTCAC
MvnI 298 CGCG CG^CG
468 CGCG
PstI 288 CTGCAG CTGCA^G
642 CTGCAG
Tsp45I 107 GTGAC ^GT[GC]AC
262 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AciI AluI AvaI BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
BsoBI Bsp1286I BssKI BstNI BstXI
Cac8I CfoI Cfr13I EarI EcoRII
Fnu4HI HaeIII HapII HhaI HinP1I
HpaII HphI ItaI Ksp632I MboII
MnlI MspA1I MspI MvaI MwoI
NciI NlaIII NlaIV PvuII Sau96I
ScrFI SfcI SmaI TseI XmaCI
XmaI