Restriction map for
pFN21AE4024
(Circular) (Six-base) MAPSORT of: pFN21AE4024.seq Check: 5731 from: 1 to: 5386
pFN21AE4024 5386 bp
With 182 enzymes: SgfI *
March 28, 2012 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2255 2255
Size: 5386
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3196 278
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2729 1937
Size: 792 4594
Acc65I G'GTAC_C
Cuts at: 2707 2707
Size: 5386
AclI AA'CG_TT
Cuts at: 1563 3513 3886 1563
Size: 1950 373 3063
Fragments arranged by size:
3063 1950 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2225 3449 4833 5101 1632
Size: 593 1224 1384 268 1917
Fragments arranged by size:
1917 1384 1224 593 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5189
AflIII A'CryG_T
Cuts at: 1203 4285 5262 1203
Size: 3082 977 1327
Fragments arranged by size:
3082 1327 977
AgeI A'CCGG_T
Cuts at: 4217 4217
Size: 5386
AhdI GACnn_n'nnGTC
Cuts at: 4115 4115
Size: 5386
AleI CACnn'nnGTG
Cuts at: 1166 2072 1166
Size: 906 4480
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5386
AlwNI CAG_nnn'CTG
Cuts at: 2371 2622 3129 4701 2371
Size: 251 507 1572 3056
Fragments arranged by size:
3056 1572 507 251
ApaI G_GGCC'C
Cuts at: 2517 2517
Size: 5386
ApaLI G'TGCA_C
Cuts at: 3443 4599 3443
Size: 1156 4230
ApoI r'AATT_y
Cuts at: 1493 2695 2921 3073 5182 1493
Size: 1202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1202 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3940 160
Size: 872 2908 1606
Fragments arranged by size:
2908 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
AvaI C'yCGr_G
Cuts at: 1870 1943 2279 2527 2610 2711 1870
Size: 73 336 248 83 101 4545
Fragments arranged by size:
4545 336 248 101 83 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2334 2367 2334
Size: 33 5353
BamHI G'GATC_C
Cuts at: 2377 2716 3083 2377
Size: 339 367 4680
Fragments arranged by size:
4680 367 339
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2463 2707 4162 618
Size: 325 217 627 676 244 1455 1842
Fragments arranged by size:
1842 1455 676 627 325 244 217
BanII G_rGCy'C
Cuts at: 1391 2142 2517 2705 1391
Size: 751 375 188 4072
Fragments arranged by size:
4072 751 375 188
BbeI G_GCGC'C
Cuts at: 2467 2467
Size: 5386
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4415
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3577 3611 1903
Size: 34 1640 34 3678
Fragments arranged by size:
3678 1640 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3278 4488 3278
Size: 1210 4176
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5386
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3997 136
Size: 107 122 71 631 605 2325 1525
Fragments arranged by size:
2325 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5381 5381
Size: 5386
BlpI GC'TnA_GC
Cuts at: 2803 2803
Size: 5386
Bme1580I G_kGCm'C
Cuts at: 1790 2517 3447 4603 1790
Size: 727 930 1156 2573
Fragments arranged by size:
2573 1156 930 727
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5386
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3117 4075 442
Size: 416 53 83 399 24 1700 958 1753
Fragments arranged by size:
1753 1700 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5386
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2138 2617 4046 1239
Size: 899 479 1429 2579
Fragments arranged by size:
2579 1429 899 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3511 4376 4674 3511
Size: 865 298 4223
Fragments arranged by size:
4223 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4049 882
Size: 617 2550 2219
Fragments arranged by size:
2550 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2452 3177 493
Size: 711 1248 725 2702
Fragments arranged by size:
2702 1248 725 711
BsaBI GATnn'nnATC
Cuts at: 5380 5380
Size: 5386
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2464 3193 3575 4210
Size: 53 83 186 936 931 729 382 635
Cuts at: 4210 275
Size: 1451
Fragments arranged by size:
1451 936 931 729 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3819 4217 4491 4638 5049 3819
Size: 398 274 147 411 4156
Fragments arranged by size:
4156 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5161
Fragments arranged by size:
5161 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2620 1186
Size: 1434 3952
BseYI C'CCAG_C
Cuts at: 2034 2084 2334 2405 2603 4589 5085 2034
Size: 50 250 71 198 1986 496 2335
Fragments arranged by size:
2335 1986 496 250 198 71 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1996 2243 1163
Size: 573 260 247 4306
Fragments arranged by size:
4306 573 260 247
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2836 3597 4625 1540
Size: 275 186 835 761 1028 2301
Fragments arranged by size:
2301 1028 835 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2550 3447 3532 4603 2550
Size: 897 85 1071 3333
Fragments arranged by size:
3333 1071 897 85
BsmI GAATG_Cn'
Cuts at: 2897 2990 2897
Size: 93 5293
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2142 2517 2550 2705 3447 3532 4603
Size: 399 352 375 33 155 742 85 1071
Cuts at: 4603 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 375 352 155 85
33
BspEI T'CCGG_A
Cuts at: 5049 5049
Size: 5386
BspHI T'CATG_A
Cuts at: 3275 5005 3275
Size: 1730 3656
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2255 2741 844
Size: 1411 486 3489
Fragments arranged by size:
3489 1411 486
BsrBI CCG'CTC
Cuts at: 1394 1823 3273 1394
Size: 429 1450 3507
Fragments arranged by size:
3507 1450 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2679 3881 4055 65
Size: 1183 1431 1202 174 1396
Fragments arranged by size:
1431 1396 1202 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4030 4217 1700
Size: 110 2220 187 2869
Fragments arranged by size:
2869 2220 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5386
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5386
BssSI C'ACGA_G
Cuts at: 3446 4458 3446
Size: 1012 4374
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5386
BstBI TT'CG_AA
Cuts at: 4190 4190
Size: 5386
BstEII G'GTnAC_C
Cuts at: 1184 2266 1184
Size: 1082 4304
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2170 2413 2074
Size: 96 243 5047
Fragments arranged by size:
5047 243 96
BstYI r'GATC_y
Cuts at: 1972 2377 2716 3083 3468 3485 4937 5023 5035
Size: 405 339 367 385 17 1452 86 12
Cuts at: 5035 5381 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 405 385 367 346 339 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2364 2364
Size: 5386
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3895
Fragments arranged by size:
3895 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2545 487
Size: 683 56 1319 3328
Fragments arranged by size:
3328 1319 683 56
BtsI GCAGTG_nn'
Cuts at: 2902 3696 3716 2902
Size: 794 20 4572
Fragments arranged by size:
4572 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2433 2468 529
Size: 35 1869 35 3447
Fragments arranged by size:
3447 1869 35 35
DraI TTT'AAA
Cuts at: 2690 2690
Size: 5386
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4393 809
Size: 507 3077 1802
Fragments arranged by size:
3077 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2469 2833 3722
Size: 54 1425 50 275 18 639 364 889
Cuts at: 3722 5351 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 639 364 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2833 1537
Size: 275 1021 4090
Fragments arranged by size:
4090 1021 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2594 2648 2680 3316 1353
Size: 1241 54 32 636 3423
Fragments arranged by size:
3423 1241 636 54 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3973 4347 4493 387
Size: 1064 138 2384 374 146 1280
Fragments arranged by size:
2384 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2138 2225 2617 3449 4046 4833 5101
Size: 393 506 87 392 832 597 787 268
Cuts at: 5101 1239
Size: 1524
Fragments arranged by size:
1524 832 787 597 506 393 392 268
87
EcoNI CCTnn'n_nnAGG
Cuts at: 2632 2632
Size: 5386
EcoO109I rG'GnC_Cy
Cuts at: 2284 2514 2284
Size: 230 5156
EcoRI G'AATT_C
Cuts at: 2695 3073 2695
Size: 378 5008
FspI TGC'GCA
Cuts at: 3892 3892
Size: 5386
HaeII r_GCGC'y
Cuts at: 1678 2467 4533 1678
Size: 789 2066 2531
Fragments arranged by size:
2531 2066 789
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2532 2564 4027 4059 4101
Size: 32 135 32 960 32 1463 32 42
Cuts at: 4101 4133 1373
Size: 32 2626
Fragments arranged by size:
2626 1463 960 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2730 2976 669
Size: 988 281 792 246 3079
Fragments arranged by size:
3079 988 792 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5386
HpaI GTT'AAC
Cuts at: 2976 2976
Size: 5386
KasI G'GCGC_C
Cuts at: 2463 2463
Size: 5386
KpnI G_GTAC'C
Cuts at: 2711 2711
Size: 5386
MfeI C'AATT_G
Cuts at: 2985 2985
Size: 5386
MluI A'CGCG_T
Cuts at: 5262 5262
Size: 5386
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4500 4684 1305
Size: 3195 184 2007
Fragments arranged by size:
3195 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3564
Fragments arranged by size:
3564 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 3344 3703 3862 518
Size: 648 906 1272 359 159 2042
Fragments arranged by size:
2042 1272 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2072 2148 2254 2329 2619 2802 3479 4627
Size: 466 76 106 75 290 183 677 1148
Cuts at: 4627 4872 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 466 290 245 183 106
76 75
NarI GG'CG_CC
Cuts at: 2464 2464
Size: 5386
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3895
Fragments arranged by size:
3895 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5386
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5386
NotI GC'GGCC_GC
Cuts at: 2833 2833
Size: 5386
NspI r_CATG'y
Cuts at: 1132 1229 2186 2744 4289 1132
Size: 97 957 558 1545 2229
Fragments arranged by size:
2229 1545 957 558 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5386
PciI A'CATG_T
Cuts at: 4285 4285
Size: 5386
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5386
PfoI T'CCnGG_A
Cuts at: 2633 2633
Size: 5386
PmeI GTTT'AAAC
Cuts at: 2690 2690
Size: 5386
PmlI CAC'GTG
Cuts at: 2452 2452
Size: 5386
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3455 3487 4968 5000 3455
Size: 32 1481 32 3841
Fragments arranged by size:
3841 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5386
PsiI TTA'TAA
Cuts at: 2956 2956
Size: 5386
PspOMI G'GGCC_C
Cuts at: 2513 2513
Size: 5386
PstI C_TGCA'G
Cuts at: 830 2297 2651 2738 830
Size: 1467 354 87 3478
Fragments arranged by size:
3478 1467 354 87
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5386
PvuII CAG'CTG
Cuts at: 2148 2329 2619 2148
Size: 181 290 4915
Fragments arranged by size:
4915 290 181
SalI G'TCGA_C
Cuts at: 1936 2728 1936
Size: 792 4594
SbfI CC_TGCA'GG
Cuts at: 2738 2738
Size: 5386
ScaI AGT'ACT
Cuts at: 3634 5346 3634
Size: 1712 3674
SexAI A'CCwGG_T
Cuts at: 2234 2234
Size: 5386
SfcI C'TryA_G
Cuts at: 826 1045 2143 2293 2647 2734 3869 4550 4741
Size: 219 1098 150 354 87 1135 681 191
Cuts at: 4741 826
Size: 1471
Fragments arranged by size:
1471 1135 1098 681 354 219 191 150
87
SfoI GGC'GCC
Cuts at: 2465 2465
Size: 5386
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5386
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5386
SmaI CCC'GGG
Cuts at: 1872 2281 2529 2612 2713 1872
Size: 409 248 83 101 4545
Fragments arranged by size:
4545 409 248 101 83
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3490 4391 4653 820
Size: 197 926 1547 901 262 1553
Fragments arranged by size:
1553 1547 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3177 493
Size: 2684 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5386
SphI G_CATG'C
Cuts at: 1132 2186 2744 1132
Size: 1054 558 3774
Fragments arranged by size:
3774 1054 558
SspI AAT'ATT
Cuts at: 5 52 3310 5
Size: 47 3258 2081
Fragments arranged by size:
3258 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5386
StyI C'CwwG_G
Cuts at: 513 1065 2004 2825 513
Size: 552 939 821 3074
Fragments arranged by size:
3074 939 821 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2533 3413 3430 3583 3768 4213 325
Size: 1255 953 880 17 153 185 445 1498
Fragments arranged by size:
1498 1255 953 880 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3632 5344 96
Size: 274 80 33 51 1443 1655 1712 138
Fragments arranged by size:
1712 1655 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2660 2692 5346 5378 2660
Size: 32 2654 32 2668
Fragments arranged by size:
2668 2654 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5386
XbaI T'CTAG_A
Cuts at: 2722 2722
Size: 5386
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2071 2071
Size: 5386
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5386
XmaI C'CCGG_G
Cuts at: 1870 2279 2527 2610 2711 1870
Size: 409 248 83 101 4545
Fragments arranged by size:
4545 409 248 101 83
XmnI GAAnn'nnTTC
Cuts at: 3515 3515
Size: 5386
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3194 276
Size: 53 83 186 936 1660 2468
Fragments arranged by size:
2468 1660 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BbvCI BfrBI BplI Bpu10I
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SrfI
SwaI