################################## # NO CUT ENZYMES ( 116 enzymes) # ################################## AatII AccI AclI AcsI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AvaI AviII AvrII BamHI BbrPI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiWI BsmI BsoBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI Ksp632I KspI MaeI MaeII MfeI MluI MseI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI SwaI Tru9I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 21 GGTACC G^GTACC AccIII 333 TCCGGA T^CCGGA Alw44I 961 GTGCAC G^TGCAC AlwI 396 GATCC GGATC(4/5) AocI 1144 CCTCAGG CC^T[ACGT]AGG ApaLI 961 GTGCAC G^TGCAC Asp718I 21 GGTACC G^GTACC BalI 99 TGGCCA TGG^CCA BbeI 1101 GGCGCC GGCGC^C BbsI 130 GTCTTC GAAGAC(2/6) BbvCI 970 CCTCAGC CCTCAGC(-5/-2) BcgI 26 CGAGAATGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglI 1382 GCCAGTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 130 GTCTTC GAAGAC(2/6) BsaBI 1152 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 274 GAGACC GGTCTC(1/5) BsaWI 333 TCCGGA [AT]^CCGG[AT] BseAI 333 TCCGGA T^CCGGA BsgI 775 GTGCAG GTGCAG(16/14) BsmBI 246 CGTCTC CGTCTC(1/5) BspEI 333 TCCGGA T^CCGGA BspHI 1088 TCATGA T^CATGA BspMI 777 GCAGGT ACCTGC(4/8) BstAPI 1015 GCAGCGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 395 AGATCC [GA]^GATC[CT] Bsu36I 1144 CCTCAGG CC^T[ACGT]AGG DpnII 396 GATC ^GATC Eco81I 1144 CCTCAGG CC^T[ACGT]AGG EcoNI 698 CCTTTGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HaeII 1101 GGCGCC [GA]GCGC^[CT] KasI 1101 GGCGCC G^GCGCC KpnI 21 GGTACC GGTAC^C MamI 1152 GATGAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboI 396 GATC ^GATC MflI 395 AGATCC [GA]^GATC[CT] MluNI 99 TGGCCA TGG^CCA MroI 333 TCCGGA T^CCGGA MscI 99 TGGCCA TGG^CCA MslI 876 CACCGGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1101 GGCGCC GG^CGCC NcoI 192 CCATGG C^CATGG NdeII 396 GATC ^GATC PpuMI 706 AGGACCC [GA]G^G[AT]CC[CT] RcaI 1088 TCATGA T^CATGA Sau3AI 396 GATC ^GATC SfiI 1381 GGCCAGTACGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1101 GGCGCC GGC^GCC SphI 881 GCATGC GCATG^C Tsp45I 428 GTGAC ^GT[GC]AC Tsp509I 1523 AATT ^AATT XcmI 1505 CCATTTCACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 395 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcyI 1101 GGCGCC G[GA]^CG[CT]C 1416 GGCGTC AlwNI 973 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG 1016 CAGCGGCTG AspHI 469 GTGCTC G[AT]GC[AT]^C 961 GTGCAC AspI 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 647 GACGCAGTC BanII 597 GGGCTC G[GA]GC[CT]^C 1052 GAGCCC BsaHI 1101 GGCGCC G[GA]^CG[CT]C 1416 GGCGTC BsiEI 37 CGGCCG CG[GA][CT]^CG 668 CGGCCG BsiHKAI 469 GTGCTC G[AT]GC[AT]^C 961 GTGCAC BsrI 1172 CCAGT ACTGG(1/-1) 1383 CCAGT DraII 706 AGGACCC [GA]G^G[ACGT]CC[CT] 1450 AGGGCCT EagI 37 CGGCCG C^GGCCG 668 CGGCCG EclXI 37 CGGCCG C^GGCCG 668 CGGCCG Eco52I 37 CGGCCG C^GGCCG 668 CGGCCG EcoO109I 706 AGGACCC [GA]G^G[ACGT]CC[CT] 1450 AGGGCCT EcoT14I 102 CCAAGG C^C[AT][AT]GG 192 CCATGG Hin1I 1101 GGCGCC G[GA]^CG[CT]C 1416 GGCGTC HphI 1149 GGTGA GGTGA(8/7) 1550 TCACC MaeIII 428 GTGAC ^GT[ACGT]AC 791 GTAAC MboII 489 GAAGA GAAGA(8/7) 131 TCTTC NaeI 39 GCCGGC GCC^GGC 1366 GCCGGC NgoMIV 39 GCCGGC G^CCGGC 1366 GCCGGC NspI 380 GCATGT [GA]CATG^[CT] 881 GCATGC PflFI 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 647 GACGCAGTC StuI 695 AGGCCT AGG^CCT 1313 AGGCCT StyI 102 CCAAGG C^C[AT][AT]GG 192 CCATGG TaqI 457 TCGA T^CGA 557 TCGA Tth111I 276 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC 647 GACGCAGTC TthHB8I 457 TCGA T^CGA 557 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AfaI AluI AvaII BanI BbvI BcnI BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DsaI EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII ItaI MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV PleI PstI PvuII RsaI Sau96I ScrFI SfaNI SfcI TfiI TseI TspRI