(Linear) (Six-base) MAPSORT of: KIBB7796.seq Check: 6861 from: 1 to: 1554 KIBB7796.orf With 182 enzymes: SgfI * December 8, 2011 13:20 .. Acc65I G'GTAC_C Cuts at: 0 21 1554 Size: 21 1533 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 652 1554 Size: 433 219 902 Fragments arranged by size: 902 433 219 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 976 1554 Size: 976 578 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 608 640 1554 Size: 608 32 914 Fragments arranged by size: 914 608 32 AlwNI CAG_nnn'CTG Cuts at: 0 978 1021 1554 Size: 978 43 533 Fragments arranged by size: 978 533 43 ApaLI G'TGCA_C Cuts at: 0 961 1554 Size: 961 593 BanI G'GyrC_C Cuts at: 0 21 1101 1179 1407 1554 Size: 21 1080 78 228 147 Fragments arranged by size: 1080 228 147 78 21 BanII G_rGCy'C Cuts at: 0 601 1056 1554 Size: 601 455 498 Fragments arranged by size: 601 498 455 BbeI G_GCGC'C Cuts at: 0 1105 1554 Size: 1105 449 BbsI GAAGACnn'nnnn_ Cuts at: 0 123 1554 Size: 123 1431 BbvCI CC'TCA_GC Cuts at: 0 971 1554 Size: 971 583 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 15 49 1554 Size: 15 34 1505 Fragments arranged by size: 1505 34 15 BglI GCCn_nnn'nGGC Cuts at: 0 1388 1554 Size: 1388 166 Bme1580I G_kGCm'C Cuts at: 0 425 446 965 1184 1412 1554 Size: 425 21 519 219 228 142 Fragments arranged by size: 519 425 228 219 142 21 BmrI ACTGGGnnnn_n' Cuts at: 0 1166 1554 Size: 1166 388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 700 709 1053 1554 Size: 700 9 344 501 Fragments arranged by size: 700 501 344 9 Bpu10I CC'TnA_GC Cuts at: 0 971 1554 Size: 971 583 BsaI GGTCTCn'nnnn_ Cuts at: 0 268 1554 Size: 268 1286 BsaBI GATnn'nnATC Cuts at: 0 1156 1554 Size: 1156 398 BsaHI Gr'CG_yC Cuts at: 0 1102 1417 1554 Size: 1102 315 137 Fragments arranged by size: 1102 315 137 BsaWI w'CCGG_w Cuts at: 0 333 1554 Size: 333 1221 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 265 295 608 638 1051 1081 1554 Size: 265 30 313 30 413 30 473 Fragments arranged by size: 473 413 313 265 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 370 511 514 697 751 1133 1554 Size: 370 141 3 183 54 382 421 Fragments arranged by size: 421 382 370 183 141 54 3 BseYI C'CCAG_C Cuts at: 0 120 389 1554 Size: 120 269 1165 Fragments arranged by size: 1165 269 120 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 796 1554 Size: 796 758 BsiEI CG_ry'CG Cuts at: 0 40 671 1554 Size: 40 631 883 Fragments arranged by size: 883 631 40 BsiHKAI G_wGCw'C Cuts at: 0 473 965 1554 Size: 473 492 589 Fragments arranged by size: 589 492 473 BsmBI CGTCTCn'nnnn_ Cuts at: 0 252 1554 Size: 252 1302 Bsp1286I G_dGCh'C Cuts at: 0 425 446 473 601 965 1056 1184 1412 Size: 425 21 27 128 364 91 128 228 Cuts at: 1412 1554 Size: 142 Fragments arranged by size: 425 364 228 142 128 128 91 27 21 BspEI T'CCGG_A Cuts at: 0 333 1554 Size: 333 1221 BspHI T'CATG_A Cuts at: 0 1088 1554 Size: 1088 466 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 768 1554 Size: 768 786 BsrFI r'CCGG_y Cuts at: 0 39 584 877 965 1342 1366 1554 Size: 39 545 293 88 377 24 188 Fragments arranged by size: 545 377 293 188 88 39 24 BstAPI GCAn_nnn'nTGC Cuts at: 0 1021 1554 Size: 1021 533 BstXI CCAn_nnnn'nTGG Cuts at: 0 324 834 1539 1554 Size: 324 510 705 15 Fragments arranged by size: 705 510 324 15 BstYI r'GATC_y Cuts at: 0 395 1554 Size: 395 1159 Bsu36I CC'TnA_GG Cuts at: 0 1145 1554 Size: 1145 409 BtgI C'CryG_G Cuts at: 0 192 237 1361 1554 Size: 192 45 1124 193 Fragments arranged by size: 1124 193 192 45 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 78 113 1554 Size: 78 35 1441 Fragments arranged by size: 1441 78 35 EaeI y'GGCC_r Cuts at: 0 37 99 668 1238 1364 1380 1389 1554 Size: 37 62 569 570 126 16 9 165 Fragments arranged by size: 570 569 165 126 62 37 16 9 EagI C'GGCC_G Cuts at: 0 37 668 1554 Size: 37 631 886 Fragments arranged by size: 886 631 37 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 154 1554 Size: 154 1400 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 433 652 700 709 1053 1554 Size: 433 219 48 9 344 501 Fragments arranged by size: 501 433 344 219 48 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 702 1554 Size: 702 852 EcoO109I rG'GnC_Cy Cuts at: 0 707 1451 1554 Size: 707 744 103 Fragments arranged by size: 744 707 103 HaeII r_GCGC'y Cuts at: 0 1105 1554 Size: 1105 449 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 265 297 583 595 615 627 1024 1056 Size: 265 32 286 12 20 12 397 32 Cuts at: 1056 1554 Size: 498 Fragments arranged by size: 498 397 286 265 32 32 20 12 12 KasI G'GCGC_C Cuts at: 0 1101 1554 Size: 1101 453 KpnI G_GTAC'C Cuts at: 0 25 1554 Size: 25 1529 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 593 634 1133 1554 Size: 593 41 499 421 Fragments arranged by size: 593 499 421 41 MscI TGG'CCA Cuts at: 0 101 1554 Size: 101 1453 MslI CAynn'nnrTG Cuts at: 0 880 1554 Size: 880 674 MspA1I CmG'CkG Cuts at: 0 120 225 389 678 834 906 1018 1218 Size: 120 105 164 289 156 72 112 200 Cuts at: 1218 1259 1269 1554 Size: 41 10 285 Fragments arranged by size: 289 285 200 164 156 120 112 105 72 41 10 NaeI GCC'GGC Cuts at: 0 41 1368 1554 Size: 41 1327 186 Fragments arranged by size: 1327 186 41 NarI GG'CG_CC Cuts at: 0 1102 1554 Size: 1102 452 NcoI C'CATG_G Cuts at: 0 192 1554 Size: 192 1362 NgoMIV G'CCGG_C Cuts at: 0 39 1366 1554 Size: 39 1327 188 Fragments arranged by size: 1327 188 39 NspI r_CATG'y Cuts at: 0 384 885 1554 Size: 384 501 669 Fragments arranged by size: 669 501 384 PasI CC'CwG_GG Cuts at: 0 1412 1448 1554 Size: 1412 36 106 Fragments arranged by size: 1412 106 36 PfoI T'CCnGG_A Cuts at: 0 398 1554 Size: 398 1156 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 608 640 1554 Size: 608 32 914 Fragments arranged by size: 914 608 32 PpuMI rG'GwC_Cy Cuts at: 0 707 1554 Size: 707 847 PstI C_TGCA'G Cuts at: 0 1017 1026 1264 1554 Size: 1017 9 238 290 Fragments arranged by size: 1017 290 238 9 PvuII CAG'CTG Cuts at: 0 120 225 389 678 834 906 1259 1554 Size: 120 105 164 289 156 72 353 295 Fragments arranged by size: 353 295 289 164 156 120 105 72 SfcI C'TryA_G Cuts at: 0 1013 1022 1260 1332 1347 1554 Size: 1013 9 238 72 15 207 Fragments arranged by size: 1013 238 207 72 15 9 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1388 1554 Size: 1388 166 SfoI GGC'GCC Cuts at: 0 1103 1554 Size: 1103 451 SphI G_CATG'C Cuts at: 0 885 1554 Size: 885 669 StuI AGG'CCT Cuts at: 0 697 1315 1554 Size: 697 618 239 Fragments arranged by size: 697 618 239 StyI C'CwwG_G Cuts at: 0 102 192 1554 Size: 102 90 1362 Fragments arranged by size: 1362 102 90 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 446 888 1341 1554 Size: 446 442 453 213 Fragments arranged by size: 453 446 442 213 TatI w'GTAC_w Cuts at: 0 359 1554 Size: 359 1195 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1051 1083 1554 Size: 1051 32 471 Fragments arranged by size: 1051 471 32 Tth111I GACn'n_nGTC Cuts at: 0 279 650 1554 Size: 279 371 904 Fragments arranged by size: 904 371 279 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1512 1554 Size: 1512 42 Enzymes that do cut: Acc65I AcuI AlfI AloI AlwNI ApaLI BanI BanII BbeI BbsI BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrFI BstAPI BstXI BstYI Bsu36I BtgI CspCI EaeI EagI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PfoI PpiI PpuMI PstI PvuII SfcI SfiI SfoI SphI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI BpuEI BsaAI BsiWI BsmI BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraI DraIII DrdI EarI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI