##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     AspEI       AspI        
AviII       AvrII       BalI        BamHI       BbeI        
BbvCI       BciVI       BclI        BfaI        BfrI        
BlnI        BlpI        Bpu1102I    BsaBI       BsaI        
BsiWI       BsmI        Bsp1407I    BspDI       BspHI       
BspLU11I    BspMI       BsrDI       BsrFI       BsrGI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DraI        DraIII      DrdI        
EaeI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        FspI        HindIII     
HpaI        KasI        KspI        MaeI        MamI        
MfeI        MluI        MluNI       MscI        MseI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmeI        PshBI       Psp1406I    PstI        PvuI        
RcaI        RsrII       SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmlI        SnaBI       SpeI        Sse8387I    
SspBI       SspI        StuI        SwaI        Tru9I       
Tth111I     Van91I      XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

Acc65I        176  GGTACC               G^GTACC
AccIII        317  TCCGGA               T^CCGGA
Aor51HI      1165  AGCGCT               AGC^GCT
Asp718I       176  GGTACC               G^GTACC
BbrPI        1128  CACGTG               CAC^GTG
BbsI         1108  GTCTTC               GAAGAC(2/6)
BcgI          986  CGATGCCCATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          347  GCCATGTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         212  AGATCT               A^GATCT
BpuAI        1108  GTCTTC               GAAGAC(2/6)
BsaWI         317  TCCGGA               [AT]^CCGG[AT]
BseAI         317  TCCGGA               T^CCGGA
BspEI         317  TCCGGA               T^CCGGA
BsrBI        1198  GAGCGG               CCGCTC(-3/-3)
BssHII         64  GCGCGC               G^CGCGC
BstXI         437  CCATTGAAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EarI          952  CTCTTC               CTCTTC(1/4)
Eco47III     1165  AGCGCT               AGC^GCT
HincII         82  GTCAAC               GT[CT]^[GA]AC
HindII         82  GTCAAC               GT[CT]^[GA]AC
KpnI          176  GGTACC               GGTAC^C
Ksp632I       952  CTCTTC               CTCTTC(1/4)
MroI          317  TCCGGA               T^CCGGA
NspI          158  GCATGC               [GA]CATG^[CT]
PleI         1324  GACTC                GAGTC(4/5)
PmaCI        1128  CACGTG               CAC^GTG
PmlI         1128  CACGTG               CAC^GTG
PshAI         328  GACAATGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SexAI         281  ACCTGGT              A^CC[AT]GGT
SfcI          616  CTACAG               C^T[GA][CT]AG
SmaI         1058  CCCGGG               CCC^GGG
SphI          158  GCATGC               GCATG^C
Tsp509I      1035  AATT                 ^AATT
XmaCI        1058  CCCGGG               C^CCGGG
XmaI         1058  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

BsaAI         902  TACGTG               [CT]AC^GT[GA]
             1128  CACGTG              
BseRI         425  CTCCTC               GAGGAG(10/8)
              769  CTCCTC              
BsgI          646  CTGCAC               GTGCAG(16/14)
             1236  CTGCAC              
BsiEI         363  CGACCG               CG[GA][CT]^CG
              803  CGGTCG              
BsmAI         116  GAGAC                GTCTC(1/5)
              799  GAGAC               
BsmBI         116  GAGACG               CGTCTC(1/5)
              799  GAGACG              
DdeI          324  CTCAG                C^T[ACGT]AG
             1169  CTGAG               
NcoI          536  CCATGG               C^CATGG
             1089  CCATGG              
PpuMI         278  AGGACCT              [GA]G^G[AT]CC[CT]
             1026  AGGACCC             
PvuII         596  CAGCTG               CAG^CTG
              957  CAGCTG              
SacI          603  GAGCTC               GAGCT^C
             1213  GAGCTC              
TfiI         1080  GATTC                G^A[AT]TC
             1386  GATTC               
XcmI          374  CCAGGGAGAAGGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              437  CCATTGAAGTGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BsrI        BssKI       
BstF5I      BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       DraII       DsaI        
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HinfI       HpaII       
HphI        ItaI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       StyI        TaqI        
TseI        Tsp45I      TspRI       TthHB8I     XhoII