Restriction map for
pFN21AE1929
(Circular) (Six-base) MAPSORT of: pFN21AE1929.seq Check: 9466 from: 1 to: 6106
pFN21AE1929 6106 bp
With 182 enzymes: SgfI *
August 16, 2010 14:53 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 2181 3427 2181
Size: 1246 4860
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2461 2944 4169 5553 5821 1632
Size: 829 483 1225 1384 268 1917
Fragments arranged by size:
1917 1384 1225 829 483 268
AfeI AGC'GCT
Cuts at: 3172 3172
Size: 6106
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 5005 5982 1203
Size: 3802 977 1327
Fragments arranged by size:
3802 1327 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 3371 1166
Size: 2205 3901
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2666 1184
Size: 1482 4624
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3113 3145 3113
Size: 32 6074
AlwNI CAG_nnn'CTG
Cuts at: 3849 5421 3849
Size: 1572 4534
ApaLI G'TGCA_C
Cuts at: 4163 5319 4163
Size: 1156 4950
ApoI r'AATT_y
Cuts at: 1493 3415 3641 3793 5902 1493
Size: 1922 226 152 2109 1697
Fragments arranged by size:
2109 1922 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 2017 2315 2881 3063 3431 1870
Size: 73 74 298 566 182 368 4545
Fragments arranged by size:
4545 566 368 298 182 74 73
BamHI G'GATC_C
Cuts at: 3436 3803 3436
Size: 367 5739
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2181 2201 3356 3427 4882
Size: 325 217 627 394 20 1155 71 1455
Cuts at: 4882 618
Size: 1842
Fragments arranged by size:
1842 1455 1155 627 394 325 217 71
20
BanII G_rGCy'C
Cuts at: 1391 2612 2853 3064 3126 3222 3266 3425 1391
Size: 1221 241 211 62 96 44 159 4072
Fragments arranged by size:
4072 1221 241 211 159 96 62 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3106 928
Size: 971 1207 3928
Fragments arranged by size:
3928 1207 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2980 3014 4297 4331 1903
Size: 34 50 34 959 34 1283 34 3678
Fragments arranged by size:
3678 1283 959 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3998 5208 3998
Size: 1210 4896
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2358 4717 136
Size: 107 122 71 631 605 686 2359 1525
Fragments arranged by size:
2359 1525 686 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2217 6101 2217
Size: 3884 2222
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2586 2914 3361 4167 5323 1790
Size: 796 328 447 806 1156 2573
Fragments arranged by size:
2573 1156 806 796 447 328
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3269 3837 4795
Size: 416 53 83 399 24 1852 568 958
Cuts at: 4795 442
Size: 1753
Fragments arranged by size:
1852 1753 958 568 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2758 2758
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2139 2544 2762 3221 3416 4766 1239
Size: 900 405 218 459 195 1350 2579
Fragments arranged by size:
2579 1350 900 459 405 218 195
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4231 5096 5394 4231
Size: 865 298 4943
Fragments arranged by size:
4943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4769 882
Size: 617 3270 2219
Fragments arranged by size:
3270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2909 3135 3897 493
Size: 711 1705 226 762 2702
Fragments arranged by size:
2702 1705 762 711 226
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 2232 2703 3050
Size: 53 83 186 936 591 108 471 347
Cuts at: 3050 3913 4295 4930 275
Size: 863 382 635 1451
Fragments arranged by size:
1451 936 863 635 591 471 382 347
186 108 83 53
BsaWI w'CCGG_w
Cuts at: 2322 4539 4937 5211 5358 5769 2322
Size: 2217 398 274 147 411 2659
Fragments arranged by size:
2659 2217 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2112 2142 2162 2192 2679
Size: 30 165 30 709 30 20 30 487
Cuts at: 2679 2709 3113 3143 1178
Size: 30 404 30 4141
Fragments arranged by size:
4141 709 487 404 165 30 30 30
30 30 30 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2421 2765 1186
Size: 1235 344 4527
Fragments arranged by size:
4527 1235 344
BseYI C'CCAG_C
Cuts at: 2832 2980 3053 5309 5805 2832
Size: 148 73 2256 496 3133
Fragments arranged by size:
3133 2256 496 148 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2636 3226 1163
Size: 573 900 590 4043
Fragments arranged by size:
4043 900 590 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2371 2811 3556 4317 5345 1540
Size: 275 186 370 440 745 761 1028 2301
Fragments arranged by size:
2301 1028 761 745 440 370 275 186
BsiHKAI G_wGCw'C
Cuts at: 2612 3222 3378 4167 4252 5323 2612
Size: 610 156 789 85 1071 3395
Fragments arranged by size:
3395 1071 789 610 156 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
BsmBI CGTCTCn'nnnn_
Cuts at: 2115 2798 2115
Size: 683 5423
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2586 2612 2853 2914 3064 3126 3222
Size: 399 796 26 241 61 150 62 96
Cuts at: 3222 3266 3361 3378 3425 4167 4252 5323 1391
Size: 44 95 17 47 742 85 1071 2174
Fragments arranged by size:
2174 1071 796 742 399 241 150 96
95 85 62 61 47 44 26 17
BspEI T'CCGG_A
Cuts at: 2322 5769 2322
Size: 3447 2659
BspHI T'CATG_A
Cuts at: 3995 5725 3995
Size: 1730 4376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3461 844
Size: 2617 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3205 3993 1394
Size: 429 1382 788 3507
Fragments arranged by size:
3507 1382 788 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 2069 1925
Size: 144 5962
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 2449
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2217 2325 2761 3436 3803 4188 4205 5657
Size: 245 108 436 675 367 385 17 1452
Cuts at: 5657 5743 5755 6101 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 675 436 385 367 346 245
108 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2541 2746 3094 513
Size: 552 1476 205 348 3525
Fragments arranged by size:
3525 1476 552 348 205
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2586 2893 3622 4416 4436 2586
Size: 307 729 794 20 4256
Fragments arranged by size:
4256 794 729 307 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2425 2460 529
Size: 35 1861 35 4175
Fragments arranged by size:
4175 1861 35 35
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3553 4442 6071
Size: 54 1425 50 275 18 1723 889 1629
Cuts at: 6071 8
Size: 43
Fragments arranged by size:
1723 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2963 4036 1353
Size: 1610 1073 3423
Fragments arranged by size:
3423 1610 1073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2015 4693 5067 5213 387
Size: 1064 138 426 2678 374 146 1280
Fragments arranged by size:
2678 1280 1064 426 374 146 138
EcoICRI GAG'CTC
Cuts at: 2610 3220 2610
Size: 610 5496
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2139 2461 2544 2762 2944 3221 3416
Size: 393 507 322 83 218 182 277 195
Cuts at: 3416 4169 4766 5553 5821 1239
Size: 753 597 787 268 1524
Fragments arranged by size:
1524 787 753 597 507 393 322 277
268 218 195 182 83
EcoO109I rG'GnC_Cy
Cuts at: 2284 2634 2953 3032 2284
Size: 350 319 79 5358
Fragments arranged by size:
5358 350 319 79
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2161 2161
Size: 6106
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2740 2847 3174 5253 1678
Size: 1062 107 327 2079 2531
Fragments arranged by size:
2531 2079 1062 327 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2746 2778 4747 4779 4821
Size: 32 135 32 1174 32 1969 32 42
Cuts at: 4821 4853 1373
Size: 32 2626
Fragments arranged by size:
2626 1969 1174 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2089 3450 3696 669
Size: 988 281 151 1361 246 3079
Fragments arranged by size:
3079 1361 988 281 246 151
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 3696 3696
Size: 6106
KpnI G_GTAC'C
Cuts at: 2185 3431 2185
Size: 1246 4860
MfeI C'AATT_G
Cuts at: 3705 3705
Size: 6106
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5220 5404 1305
Size: 3915 184 2007
Fragments arranged by size:
3915 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4284
Fragments arranged by size:
4284 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2447 2675 3371 4064 4423 4582 518
Size: 648 1281 228 696 693 359 159 2042
Fragments arranged by size:
2042 1281 696 693 648 359 228 159
MspA1I CmG'CkG
Cuts at: 1606 2031 2603 2832 2964 3522 4199 5347 5592
Size: 425 572 229 132 558 677 1148 245
Cuts at: 5592 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 572 558 425 245 229
132
NcoI C'CATG_G
Cuts at: 513 1065 2541 3094 513
Size: 552 1476 553 3525
Fragments arranged by size:
3525 1476 553 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2167 3464 5009 1132
Size: 97 938 1297 1545 2229
Fragments arranged by size:
2229 1545 1297 938 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 5005 5005
Size: 6106
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6106
PfoI T'CCnGG_A
Cuts at: 2058 2697 3117 3159 2058
Size: 639 420 42 5005
Fragments arranged by size:
5005 639 420 42
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PmlI CAC'GTG
Cuts at: 3135 3135
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3113 3145 4175 4207 5688 5720 3113
Size: 32 1030 32 1481 32 3499
Fragments arranged by size:
3499 1481 1030 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2284 3032 2284
Size: 748 5358
PshAI GACnn'nnGTC
Cuts at: 1613 2337 1613
Size: 724 5382
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2603 2964 2603
Size: 361 5745
SacI G_AGCT'C
Cuts at: 2612 3222 2612
Size: 610 5496
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 4354 6066 4354
Size: 1712 4394
SexAI A'CCwGG_T
Cuts at: 2286 2286
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 2621 3454 4589 5270 5461 826
Size: 219 1576 833 1135 681 191 1471
Fragments arranged by size:
1576 1471 1135 833 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3065 3433 1872
Size: 1193 368 4545
Fragments arranged by size:
4545 1193 368
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4210 5111 5373 820
Size: 197 926 2267 901 262 1553
Fragments arranged by size:
2267 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 2167 3464 1132
Size: 1035 1297 3774
Fragments arranged by size:
3774 1297 1035
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6106
StyI C'CwwG_G
Cuts at: 513 1065 2541 3094 3369 3545 513
Size: 552 1476 553 275 176 3074
Fragments arranged by size:
3074 1476 553 552 275 176
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2554 2994 4133 4150 4303 4488 4933
Size: 1255 974 440 1139 17 153 185 445
Cuts at: 4933 325
Size: 1498
Fragments arranged by size:
1498 1255 1139 974 445 440 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4352 6064 96
Size: 274 80 33 51 1443 2375 1712 138
Fragments arranged by size:
2375 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2112 2144 2162 2194 2679 2711 6066 6098 2112
Size: 32 18 32 485 32 3355 32 2120
Fragments arranged by size:
3355 2120 485 32 32 32 32 18
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2386 2449 2386
Size: 63 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3063 3431 1870
Size: 1193 368 4545
Fragments arranged by size:
4545 1193 368
XmnI GAAnn'nnTTC
Cuts at: 4235 4235
Size: 6106
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI